Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 170,838 G→T 7.2% intergenic (+19/‑551) PP_0159 → / → PP_16SA family III CoA‑transferase/16S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947170,8380GT 7.2% 434.6 / 19.0 157intergenic (+19/‑551)PP_0159/PP_16SAfamily III CoA‑transferase/16S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (63/82);  new base T (5/7);  total (68/89)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.79e-01

Only 100 of 158 total aligned reads displayed.

TCGGAGACGCCAGTGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGTTAAGTAGTTAAGG  >  NC_002947/170691‑170986
                                                                                                                                                   |                                                                                                                                                    
tCGGAGACGCCAGTGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTc                                                                                                                                                    <  1:3176356/150‑1 (MQ=255)
tCGGAGACGCCAGTGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTc                                                                                                                                                    <  2:3785270/150‑1 (MQ=255)
 cGGAGACGCCAGTGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCa                                                                                                                                                   <  2:646969/150‑1 (MQ=255)
 cGGAGACGCCAGTGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCa                                                                                                                                                   <  2:2958786/150‑1 (MQ=255)
   gagaCGCCAGTGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGGGGCCGTCAgg                                                                                                                                                 >  1:1514474/1‑150 (MQ=255)
   gagaCGCCAGTGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGCGGCCGTCAgg                                                                                                                                                 >  1:1514270/1‑150 (MQ=255)
    agaCGCCAGTGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGc                                                                                                                                                <  2:3620554/150‑1 (MQ=255)
     gaCGCCAGTGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGcc                                                                                                                                               >  2:3920333/1‑150 (MQ=255)
        gCCAGTGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTcc                                                                                                                                            <  2:2388894/150‑1 (MQ=255)
          cAGTGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTcccc                                                                                                                                          >  2:3788898/1‑150 (MQ=255)
            gTGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCTGACGGCCTCCCCGc                                                                                                                                        <  2:3475195/150‑1 (MQ=255)
            gTGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGc                                                                                                                                        <  2:3739668/150‑1 (MQ=255)
            gTGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGc                                                                                                                                        <  2:1786538/150‑1 (MQ=255)
              ggAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGTGGGGAGGCCTGACGGCCTCCCCGCtt                                                                                                                                      <  2:1810195/150‑1 (MQ=255)
              ggAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGTGGGGAGGCCTGACGGCCTCCCCGCtt                                                                                                                                      <  2:1709196/150‑1 (MQ=255)
                  tATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGtt                                                                                                                                  >  2:1085032/1‑150 (MQ=255)
                         gggCGCCACCACTGCTGGGCGAGCATCCCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAgc                                                                                                                           <  2:3238817/150‑1 (MQ=255)
                         gggCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAgc                                                                                                                           <  2:987057/150‑1 (MQ=255)
                             gcCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGGGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTa                                                                                                                       >  1:2663150/1‑150 (MQ=255)
                             gcCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTAAGCGCTa                                                                                                                       >  1:3797605/1‑150 (MQ=255)
                             gcCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGAGGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTa                                                                                                                       <  2:3175455/150‑1 (MQ=255)
                                accacTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCa                                                                                                                    >  2:3185578/1‑150 (MQ=255)
                                accacTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCGTTGTTTTTCCGCGCTATCa                                                                                                                    >  2:4149871/1‑150 (MQ=255)
                                  cacgggtgGGCGCGCATACCTCAGTGTGTCTGGGGGTTGTGTTGGGGCTGGTTGGTGGCGTGGTACCGGGTCGGCGTGATGGTGGGGTGCTCTATTTGAAACGGGTGGTGGCCTTCTGGCCTCCCCGCTTTGTTTTTCAGCGCTATCaaa                                                                                                                  <  1:3638144/144‑1 (MQ=255)
                                  cacTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCaaa                                                                                                                  >  1:850794/1‑150 (MQ=255)
                                    ggggggggCGCGCATACGGTGGGGGTGGGGGGGGAGGTGTGGGGGGGGGTTGCGGGGGTGGTACCGGGTCTGGGTGATGGGGGGGTGCTCTAGATGAAAGGGGGGGCGGCCGTCCGGCCCCCCCGCTTTGTTTTTCAGCGCTATCAAAgg                                                                                                                <  1:2133487/145‑1 (MQ=255)
                                       ctgGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCGCTAGATGAAAAGCGGGGGGGCCGGCCGGCCGCCCCGCGTTGGTTTTCAGCGCTATCAAAGGtca                                                                                                             >  1:2405410/1‑148 (MQ=255)
                                         gggCGAGCATACCGATGTGGTGCTGGGGGATGTGTTGGGGGTGGTTGCTGGCGAGTTACTGCGTCTGCGTGATGGTGGGGTGCTCTAGATTAAAAGGGGGGAGGCGGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAAtt                                                                                                           <  1:2874220/150‑1 (MQ=255)
                                         gggCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAAtt                                                                                                           <  2:728696/150‑1 (MQ=255)
                                         gggCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCGTTGTTTTTCAGCGCTATCAAAGGTAAtt                                                                                                           >  2:1828846/1‑150 (MQ=255)
                                             gAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTGTGTTTTTCAGCGCTATCAAAGGTAATTGaaa                                                                                                       >  2:2426192/1‑150 (MQ=255)
                                             gAGCATACCGAAGTGGTGCGGGGGGATGTGTTGGGGCTGGATGCTGGCGTGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGTGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGaaa                                                                                                       <  2:3800856/150‑1 (MQ=255)
                                              aGCATACCGACGGGGTGCGGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGAGGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAAt                                                                                                      <  2:2466908/150‑1 (MQ=255)
                                                  tACCGATGTGGTGCTGGGGGATGTGTGGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTCGATGAAACGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTaaa                                                                                                  <  2:1618603/150‑1 (MQ=255)
                                                  tACCGAAGTGGTGGTGGGGGATGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTaaa                                                                                                  <  2:62946/150‑1 (MQ=255)
                                                  tACCGAAGTGGTGCTGGGGGATGTGTTGGGGGTGGATGCTGGCGAGTTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGCCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTaaa                                                                                                  <  2:3781231/150‑1 (MQ=255)
                                                  tACCGAAGTGGTGCTGGGGGATGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTaaa                                                                                                  <  2:3216016/150‑1 (MQ=255)
                                                  tACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGGGTGATGCTGGGGTGCTCTTGATGAAAAGCGGGGCGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTaaa                                                                                                  <  1:4425479/150‑1 (MQ=255)
                                                  tACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTaaa                                                                                                  <  2:479444/150‑1 (MQ=255)
                                                   agcGAAGTGGTGCTGGGGGTTGTGTTGGGGGTGGATGCTGGCGAGGTACTGCGTCTGCGTGTTGCTGGGGTGCTCTTGATGAAAAGCGGGGAGGCCGTCAGGGCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAg                                                                                                 <  1:3346747/148‑1 (MQ=255)
                                                   aCCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAg                                                                                                 <  2:2945720/150‑1 (MQ=255)
                                                   aCCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCGCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAg                                                                                                 >  1:2895570/1‑150 (MQ=255)
                                                       aaGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAATGTAATTTAAATTAAAGGttt                                                                                             >  2:894417/1‑149 (MQ=255)
                                                               cTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCctc                                                                                     >  2:3916364/1‑150 (MQ=255)
                                                                 gggggAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCtctc                                                                                   <  2:1428024/150‑1 (MQ=255)
                                                                  ggggAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCg                                                                                  >  2:2039743/1‑150 (MQ=255)
                                                                     gAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAAt                                                                               >  2:832612/1‑150 (MQ=255)
                                                                        gtgtTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATccc                                                                            <  2:2560861/150‑1 (MQ=255)
                                                                         tgtTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATcccc                                                                           <  2:3294293/150‑1 (MQ=255)
                                                                          gtTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCGATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCt                                                                          >  1:2607393/1‑150 (MQ=255)
                                                                           ttGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGGGGCCGTCACGCCTCCCCGCGTTGTTTTTAAGCGCTATCAAATGTAAGTGTAATTAAATGTTGTCGCCCACTCGAATacccgg                                                                         >  1:122850/1‑148 (MQ=255)
                                                                           ttGGGGCTGGATGCCGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCtt                                                                         <  2:2580320/150‑1 (MQ=255)
                                                                                 cTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCCGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCGTATAATg                                                                   >  1:2212300/1‑150 (MQ=255)
                                                                                 cTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCCGCGCTATCAAAGGTAATTGAAATTAAAGGTTGAAGCCCTCTCGAATCCCCGGATAATg                                                                   >  2:4158443/1‑150 (MQ=255)
                                                                                  tGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGGGGCCGTAAGGCCACCCCGCGTTGTTTTTCAGCCCGTAAAAAAGTAAATGAAATTAAATGTTTAAGCCCACACCAAAACCCATATaaata                                                                  >  1:848713/1‑147 (MQ=255)
                                                                                    gATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATgcgc                                                                <  1:4516090/150‑1 (MQ=255)
                                                                                       gCTGGCGTGGTACAGGGTCTGCGTGATGGTGGGGTGCTCTAGATGAAACGCGGGGAGGCCGTCTGGCCCCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGcccc                                                             <  1:2107468/150‑1 (MQ=38)
                                                                                         tGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGCGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATAAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATACCCGTATAATGCGCCCCAc                                                           >  1:3583204/1‑150 (MQ=255)
                                                                                         tGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCAc                                                           >  2:2705079/1‑150 (MQ=255)
                                                                                         tGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAAATGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCAc                                                           >  2:4090515/1‑150 (MQ=255)
                                                                                         gggCGTGGTACCGGGTGTGGGTGTTGGGGGGGTGCTCTAGTTGAACCGGGGGGTGGCGGTCCGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCAc                                                           <  1:2054718/149‑1 (MQ=21)
                                                                                              tggTACTGCGTCTGCGTTTTGCGGGGGTGCTCTCGTTGAAAAGGGGGGCGGCCGTCAGGCGTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCa                                                      <  1:2125244/149‑1 (MQ=25)
                                                                                                gTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGc                                                    <  2:2677657/150‑1 (MQ=255)
                                                                                                gTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGc                                                    >  2:1460743/1‑150 (MQ=255)
                                                                                                   cAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTAGAATCCCCATATAATGCGCCCCAATTACAGCGaa                                                 >  2:3648480/1‑149 (MQ=255)
                                                                                                      cGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATc                                              <  2:2465980/150‑1 (MQ=255)
                                                                                                      cGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATc                                              <  2:208221/150‑1 (MQ=255)
                                                                                                         cTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGa                                           <  2:3897117/150‑1 (MQ=255)
                                                                                                          tGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGaa                                          <  1:3223825/150‑1 (MQ=255)
                                                                                                           gCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTGAGGCCTCCCCCCGTTGTTTTTCATCGCTATCAAAAGTAAATTAAATTAAAGGTTTAAGCCCAAAAGAATACCCGTAAAATGCGCCCCACATACAGCGAAAAAGGAAc                                         >  1:3233691/1‑150 (MQ=255)
                                                                                                                 tGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGGCTCCCCGGTTTGTTTTTGAGGGCTATCAAAGGTAATTGAAATTAAATGTTGTCGCCCTCTCGAATACCCTTATAATGCGCCCCACTTCCAGCGAAATAGGAAAGaaaaa                                   >  1:4519190/1‑150 (MQ=25)
                                                                                                                 tGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGaaaaa                                   <  1:3711342/150‑1 (MQ=255)
                                                                                                                 tGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAACTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGaaaaa                                   <  1:3711330/150‑1 (MQ=255)
                                                                                                                     ggggTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTcc                               <  1:4450522/150‑1 (MQ=255)
                                                                                                                     ggggTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTcc                               <  1:1460743/150‑1 (MQ=255)
                                                                                                                      gggTGCTCTAGTTGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCt                              <  2:1431289/150‑1 (MQ=255)
                                                                                                                       gttGCTATATATTAAATGCGGGGAGGCGGTCTGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCtt                             <  1:1457367/148‑1 (MQ=21)
                                                                                                                           ctctAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTgaga                         <  2:2234415/150‑1 (MQ=255)
                                                                                                                            tctAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAATGTAAATTAAATTAAATGTTGAAGCCCTCTCGAATACCCATATAATTCGCCCCACATCCAGCGACATCGGAACGAAAAACTCCATGAGAt                        >  1:2585134/1‑150 (MQ=25)
                                                                                                                            tctAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGAt                        >  2:4445907/1‑150 (MQ=255)
                                                                                                                            tctAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTAAGCGCTATAAAAGGTAATTGAAATTAAAGGTTGAAGCCCAAACGAATCCCCTTATAATGCGCCCCACTTACAGCGACAACGGAACGAAAAACTCCATGAGAt                        >  1:1861683/1‑150 (MQ=25)
                                                                                                                              ttgATTAAACGGGGGGAGGCCGTCAGGCCGCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTc                      <  1:3788898/148‑1 (MQ=255)
                                                                                                                              tAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATTCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTc                      >  2:4411571/1‑150 (MQ=255)
                                                                                                                                gATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCaa                    <  1:2469026/150‑1 (MQ=255)
                                                                                                                                gATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCaa                    <  2:3518827/150‑1 (MQ=255)
                                                                                                                                gATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCaa                    <  2:1241922/150‑1 (MQ=255)
                                                                                                                                aaaacaaaGCGGGGAGGGCTGACGGCCTCCCCGCTTTGTTTTTCAGGGCTATGAAATGTAATTTTAATTAAAGGTTTACGCCCTCTCGAATACCCTTATAATGGGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCaa                    >  2:864066/6‑150 (MQ=17)
                                                                                                                                   gAAAAGCGGGGCGGCCGTCAGGCCGCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGa                 <  1:4233259/150‑1 (MQ=255)
                                                                                                                                   gAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTACAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGa                 <  2:4220179/150‑1 (MQ=255)
                                                                                                                                     aaaGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGt               <  1:1085032/150‑1 (MQ=255)
                                                                                                                                         cGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGTTAag           <  2:2732999/150‑1 (MQ=255)
                                                                                                                                         cGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCAATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGTTAag           >  1:571441/1‑150 (MQ=255)
                                                                                                                                          ggggAGGCCTGACGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATCATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGTTAagt          >  2:4505319/1‑150 (MQ=18)
                                                                                                                                          ggggAGGCCTGACGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGTTAagt          >  2:3887767/1‑150 (MQ=255)
                                                                                                                                            ggAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGTTAagtag        >  1:2485066/1‑150 (MQ=255)
                                                                                                                                              aGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGTTAAGTAGtt      <  2:95115/150‑1 (MQ=255)
                                                                                                                                                gCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGTTAAGTAGTTaa    <  1:3185578/150‑1 (MQ=255)
                                                                                                                                                  cGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGTTAAGTAGTTAAgg  >  1:2390138/1‑150 (MQ=255)
                                                                                                                                                  cGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGTTAAGTAGTTAAgg  <  2:1514270/150‑1 (MQ=255)
                                                                                                                                                  cGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGTTAAGTAGTTAAgg  <  2:1514474/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TCGGAGACGCCAGTGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGTTAAGTAGTTAAGG  >  NC_002947/170691‑170986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑