BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	873,421	G→A	5.2%	G18S (GGC→AGC)	PP_5457 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	873,421	0	G	A	5.2%	621.9 / 13.3	190	G18S (GGC→AGC)	PP_5457	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (85/95); new base A (6/4); total (91/99)
Fisher's exact test for biased strand distribution p-value = 5.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 211 total aligned reads displayed.

ACCGAGGATTGCCAGGTGTGCTGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATCGATGCGTACAA  >  NC_002947/873272‑873569

aCCGAGGATTGCCAGGTGTGCTGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTggcag                                                                                                                                                      >  1:2599445/1‑150 (MQ=255)

 ccGAGGATTGCCAGGTGTGCTGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTggcagg                                                                                                                                                     >  1:2869350/1‑150 (MQ=255)

  cGAGGATTGCCAGGTGTGCTGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTggcaggc                                                                                                                                                    <  2:4179556/150‑1 (MQ=255)

   gAGGATTGCCAGGTGTGCTGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTggcaggca                                                                                                                                                   >  1:2726734/1‑150 (MQ=255)

       aTTGCCAGGTGTGCTGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCt                                                                                                                                               <  1:327144/150‑1 (MQ=255)

       aTTGCCAGGTGTGCTGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCt                                                                                                                                               <  1:1465863/150‑1 (MQ=255)

        ttGCCAGGTGTGCTGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCtt                                                                                                                                              <  2:1018478/150‑1 (MQ=255)

            cAGGTGTGCTGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAAGCATGCCTGCCa                                                                                                                                          <  1:1377563/150‑1 (MQ=255)

               gtgtGCTGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCg                                                                                                                                       >  2:4126069/1‑150 (MQ=255)

                  tgCTGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAgg                                                                                                                                    >  1:784440/1‑150 (MQ=255)

                     tGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGa                                                                                                                                 >  1:1113778/1‑150 (MQ=255)

                     tGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGa                                                                                                                                 >  1:703529/1‑150 (MQ=255)

                      gTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGAt                                                                                                                                >  2:1290536/1‑150 (MQ=255)

                       tCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATc                                                                                                                               >  2:2720132/1‑150 (MQ=255)

                          gCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGAt                                                                                                                            <  2:3829526/150‑1 (MQ=255)

                          gCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGAt                                                                                                                            >  1:4418145/1‑150 (MQ=255)

                          gCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGAt                                                                                                                            <  2:1497285/150‑1 (MQ=255)

                             tgtgGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTa                                                                                                                         >  1:565574/1‑150 (MQ=255)

                               tggtgTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTaca                                                                                                                       >  1:4309372/1‑150 (MQ=255)

                                ggtgTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTacac                                                                                                                      <  1:1634909/150‑1 (MQ=255)

                                ggtgTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTacac                                                                                                                      <  1:3571933/150‑1 (MQ=255)

                                  tgtgCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGACGCGAGGACGAAACATGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGt                                                                                                                    >  1:1403532/1‑150 (MQ=255)

                                    tgCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTgg                                                                                                                  <  2:2816802/150‑1 (MQ=255)

                                       aTTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCg                                                                                                               >  1:930855/1‑150 (MQ=255)

                                        ttCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCgg                                                                                                              >  2:672491/1‑150 (MQ=255)

                                              aGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGa                                                                                                        >  2:4328420/1‑150 (MQ=255)

                                              aGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGa                                                                                                        <  1:1272537/150‑1 (MQ=255)

                                              aGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTAAACGTGGTCGGCCGTGa                                                                                                        <  1:3165922/150‑1 (MQ=255)

                                               ggTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGAc                                                                                                       <  1:2999573/150‑1 (MQ=255)

                                                gTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGAcc                                                                                                      >  2:747782/1‑150 (MQ=255)

                                                gTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGAcc                                                                                                      >  2:3596181/1‑150 (MQ=255)

                                                  gCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCtt                                                                                                    <  1:167948/150‑1 (MQ=255)

                                                    aTGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGt                                                                                                  <  2:4111915/150‑1 (MQ=255)

                                                        tGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGGGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGt                                                                                              <  1:2376134/150‑1 (MQ=255)

                                                        tGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGt                                                                                              <  1:3778493/150‑1 (MQ=255)

                                                        tGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGt                                                                                              <  1:3680544/150‑1 (MQ=255)

                                                           cGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTgcc                                                                                           >  2:1870799/1‑150 (MQ=255)

                                                            gAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTgccg                                                                                          >  2:3065476/1‑150 (MQ=255)

                                                            gAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTgccg                                                                                          >  1:195098/1‑150 (MQ=255)

                                                             aaTGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGTCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTgccgc                                                                                         >  2:4045025/1‑150 (MQ=255)

                                                               tGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGccgccg                                                                                       <  2:1575385/150‑1 (MQ=255)

                                                                ggATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCgg                                                                                      <  1:2508383/150‑1 (MQ=255)

                                                                 gATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGc                                                                                     <  1:3234046/150‑1 (MQ=255)

                                                                  aTGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCg                                                                                    <  2:1329250/150‑1 (MQ=255)

                                                                     cTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCGGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGc                                                                                 >  1:2311674/1‑150 (MQ=255)

                                                                     cTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGc                                                                                 <  1:1501472/150‑1 (MQ=255)

                                                                     cTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGc                                                                                 <  1:3140503/150‑1 (MQ=255)

                                                                      ttGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCt                                                                                >  1:2193486/1‑150 (MQ=255)

                                                                       tGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTg                                                                               <  2:1727397/150‑1 (MQ=255)

                                                                        gAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGc                                                                              >  1:1394728/1‑150 (MQ=255)

                                                                          aGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGccc                                                                            <  1:1096973/150‑1 (MQ=255)

                                                                          aGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGccc                                                                            <  1:1858534/150‑1 (MQ=255)

                                                                            accgccgCGAGCACGAAACCTGATGCAGCGTATCTACGAACCGGAAAGCCTCCTGGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATCTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCta                                                                          >  2:3356648/2‑149 (MQ=255)

                                                                             ccgccgCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTg                                                                         <  1:102859/150‑1 (MQ=255)

                                                                               gccgcGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCa                                                                       >  1:4468033/1‑150 (MQ=255)

                                                                               gccgcGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCa                                                                       >  2:1834903/1‑150 (MQ=255)

                                                                                ccgcGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAg                                                                      <  1:2006548/150‑1 (MQ=255)

                                                                                 cgcgAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAgg                                                                     <  2:2467790/150‑1 (MQ=255)

                                                                                  gcgAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAggg                                                                    >  2:4473649/1‑150 (MQ=255)

                                                                                   cgAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGc                                                                   <  1:3335138/150‑1 (MQ=255)

                                                                                      ggACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCctg                                                                <  1:1816687/150‑1 (MQ=255)

                                                                                         cgacACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCctgctg                                                             >  2:287931/1‑150 (MQ=255)

                                                                                          gacACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTgg                                                            >  2:2827839/1‑150 (MQ=255)

                                                                                            cacCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTgggg                                                          <  2:2441893/150‑1 (MQ=255)

                                                                                            cacCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTgggg                                                          <  2:726260/150‑1 (MQ=255)

                                                                                              ccTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCt                                                        >  2:612423/1‑150 (MQ=255)

                                                                                               cTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCtc                                                       >  1:3461888/1‑150 (MQ=255)

                                                                                                       gCGTATCTACGAACCGGAAAGCCTTCTTGAGGTGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGc                                               <  1:4518447/150‑1 (MQ=255)

                                                                                                       gCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGc                                               >  2:2407726/1‑150 (MQ=255)

                                                                                                               aCGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGc                                       >  1:2207485/1‑150 (MQ=255)

                                                                                                               aCGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGc                                       >  1:2043586/1‑150 (MQ=255)

                                                                                                                 gAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAg                                     >  2:30240/1‑150 (MQ=255)

                                                                                                                 gAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAg                                     >  1:1724649/1‑150 (MQ=255)

                                                                                                                  aaCCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAgg                                    <  1:97984/150‑1 (MQ=255)

                                                                                                                   aCCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGc                                   <  1:675880/150‑1 (MQ=255)

                                                                                                                          aGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATTAGCAGGCCGAATgc                            >  2:2265671/1‑150 (MQ=255)

                                                                                                                            ccTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATgcgc                          <  1:300081/150‑1 (MQ=255)

                                                                                                                             cTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCa                         <  1:3053/150‑1 (MQ=255)

                                                                                                                              tGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCAc                        <  2:3603565/150‑1 (MQ=255)

                                                                                                                                cTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAg                                                                                  >  2:123651/1‑90 (MQ=255)

                                                                                                                                cTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAg                                                                                  <  1:123651/90‑1 (MQ=255)

                                                                                                                                    aGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGc                  >  2:2138636/1‑150 (MQ=255)

                                                                                                                                       tcccctcgCTGGCAAGCATGCCTGCCAGCAAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGa               >  1:616815/8‑150 (MQ=255)

                                                                                                                                        gcAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGAt              >  1:3974783/1‑150 (MQ=255)

                                                                                                                                        gcAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGAt              >  1:4384033/1‑150 (MQ=255)

                                                                                                                                         ccctcgCTGGCAAGCATGCCTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATc             >  1:58586/6‑150 (MQ=255)

                                                                                                                                         cAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATc             <  1:383227/150‑1 (MQ=255)

                                                                                                                                         cAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATc             <  1:2562222/150‑1 (MQ=255)

                                                                                                                                          aaaTGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATCg            >  1:228173/1‑150 (MQ=255)

                                                                                                                                           ctcgCTGGCAAGCATGCCTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATCGa           >  1:1913295/4‑150 (MQ=255)

                                                                                                                                           aaTGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATCGa           >  2:3270833/1‑150 (MQ=255)

                                                                                                                                            tcgCTGGCAAGCATGCCTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATCGAt          >  2:2918531/3‑150 (MQ=255)

                                                                                                                                            tcgCTGGCAAGCATGCCTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATCGAt          >  1:3384460/3‑150 (MQ=255)

                                                                                                                                               cTGGCAAGCATGCCTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATCGATGCg       >  2:868510/1‑150 (MQ=255)

                                                                                                                                                 ggcaggcaTGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATCGATGCGTa     <  2:2726734/150‑1 (MQ=255)

                                                                                                                                                  gcaggcaTGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATCGATGCGTAc    >  1:404495/1‑150 (MQ=255)

                                                                                                                                                  gcaggcaTGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATCGATGCGTAc    >  1:410898/1‑150 (MQ=255)

                                                                                                                                                    aggcaTGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATCGATGCGTACaa  >  2:365986/1‑150 (MQ=255)

                                                                                                                                                    aggcaTGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATCGATGCGTACaa  >  2:3321492/1‑150 (MQ=255)

                                                                                                                                                    aggcaTGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATCGATGCGTACaa  >  2:1994432/1‑150 (MQ=255)

ACCGAGGATTGCCAGGTGTGCTGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATCGATGCGTACAA  >  NC_002947/873272‑873569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`