Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 968,881 A→C 11.0% intergenic (‑311/+20) PP_0831 ← / ← PP_t68 hypothetical protein/tRNA‑Leu

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947968,8810AC11.0% 349.7 / 24.5 120intergenic (‑311/+20)PP_0831/PP_t68hypothetical protein/tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base A (73/33);  new base C (6/8);  total (79/41)
Fisher's exact test for biased strand distribution p-value = 7.24e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

AATACTTTCGGAATATATCTGACGAATGACAGCTTAAGTGCTAGCGCAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGCTGGTCGGTCAATCGGC  >  NC_002947/968733‑969028
                                                                                                                                                    |                                                                                                                                                   
aaTACTTTCGGAATATATCTGACGAATGACAGCTTAAGTGCTAGCGCAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAg                                                                                                                                                    >  1:824104/1‑150 (MQ=255)
aaTACTTTCGGAATATATCTGACGAATGACAGCTTAAGTGCTAGCGCAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAg                                                                                                                                                    >  1:3398970/1‑150 (MQ=255)
         gtaaTATATCCGACGAATGACAGCTTAAGCGCTAGCGCCAGGGGCGGCCAATAGACTGCAGGTCTGGGGGGCGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGtttt                                                                                                                                           <  2:1854388/148‑1 (MQ=255)
         ggAATATATCCGACGAATGACAGCTTAAGCGCGAGCGCCAGGGGCGGCCAATAGACTGCAGGCCGGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCTTACGGTCGGtttt                                                                                                                                           <  2:17911/150‑1 (MQ=255)
         ggAATATAACTGACGAATGACAGCTCAAGCGCCAGCGCAAGGGGCGGCCAAGAGAAGGCAGGCAGGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCTTACGGTCGGtttt                                                                                                                                           <  2:18013/150‑1 (MQ=255)
          gAATATATCTGACGAATGACAGCTTAAGTGCTAGCGCAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGttttt                                                                                                                                          >  2:2521881/1‑150 (MQ=255)
          gAATATATCTGACGAATGACAGCTTAAGTGCTAGCGCAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGttttt                                                                                                                                          >  2:263939/1‑150 (MQ=255)
          gAATATATCTGACGAATGACAGCTTAAGTGCTAGCGCAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGttttt                                                                                                                                          >  1:3890485/1‑150 (MQ=255)
            atatatCTGACGAATGACAGCTTAAGTGCTAGCGCACGGGGCGGCCAAGAGACTGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCTTACGGTCGGttttttt                                                                                                                                        <  1:1001316/150‑2 (MQ=255)
             tacagcTGCCGACTGCCCGCTTACGTGCTCGCGCACGGGGCGGCCCATCGACGGCAGGTCGGGGGGGCGGCGCGGGCGACCGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTcc                                                                                                                                       <  1:377303/145‑1 (MQ=255)
                   tGACGAATGACAGCTTAAGTGCTAGCGCAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGAttt                                                                                                                                 >  2:13422/1‑150 (MQ=255)
                   tGACGAATGACAGCTTAAGTGCTAGCGCAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGAttt                                                                                                                                 >  2:3732895/1‑150 (MQ=255)
                   tGACGAATGACAGCTTAAGTGCTAGCGCAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGAttt                                                                                                                                 >  1:4142977/1‑150 (MQ=255)
                   cgACGAATGACAGCTCAAGTGCTAGCGCCAGGGGCGGCCAAGAGACGGCAGGTCGGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGAttt                                                                                                                                 <  2:3269764/149‑1 (MQ=255)
                         aTGACCGCTTAAGTGCTAGCGCACGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCCGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGcc                                                                                                                           <  2:3338774/150‑1 (MQ=255)
                          tGACAGCTTAAGTGCTAGCGCAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGccc                                                                                                                          >  1:4408867/1‑150 (MQ=255)
                          tGACAGCTTAAGTGCTAGCGCAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGccc                                                                                                                          >  2:1517577/1‑150 (MQ=255)
                          tGACAGCTTAAGTGCTAGCGCAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCAGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGCCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGccc                                                                                                                          >  1:1977621/1‑150 (MQ=255)
                           gACAGCTTAAGTGCTAGCGCAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGcccc                                                                                                                         >  2:3861081/1‑150 (MQ=255)
                            aCAGCTTAAGTGCTAGCGCAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCg                                                                                                                        >  2:2261338/1‑150 (MQ=255)
                            aCAGCTTAAGTGCTAGCGCAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGCAAGGTCGGTTTTTTCCGGATTTGGTGCCCCg                                                                                                                        >  1:4379517/1‑150 (MQ=255)
                              aGCTTAAGTGCTAGCGCAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAg                                                                                                                      >  1:3637508/1‑150 (MQ=255)
                              aGCTGAAGTGCTAGCGCAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTACGGTCGGTTTTTTCCGGAGTTGGTGCCCCGAg                                                                                                                      >  1:1980793/1‑150 (MQ=255)
                                cTTAAGTGCTAGCGCAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAggg                                                                                                                    >  2:3479469/1‑150 (MQ=255)
                                 ttAAGTGCTAGCGCAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTCGGTGCCCCGAGGga                                                                                                                   >  2:2307452/1‑150 (MQ=255)
                                 ttAAGTGCCAGCGCACGGGGCGGCCAATCGACACCAGGTCTGGGGGGAGGCGCGGGAGCCAGGGGGGCGGCGACACCGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGga                                                                                                                   <  2:1170358/150‑1 (MQ=255)
                                         tAGCGCAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGaa                                                                                                           >  2:2776472/1‑150 (MQ=255)
                                           gcgcAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGGGACTCGAACt                                                                                                         >  1:565319/1‑150 (MQ=255)
                                             gcAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTcc                                                                                                       >  1:1750552/1‑150 (MQ=255)
                                                   ggcggcCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTcc                                                                                                 >  1:3366685/1‑150 (MQ=255)
                                                      gcccAATAGCCTGCCGGTCGGGGGGGACGCGCGGGAGCCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCttt                                                                                              <  2:76632/148‑1 (MQ=255)
                                                        ccAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCg                                                                                            >  2:244572/1‑150 (MQ=255)
                                                        ccAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCg                                                                                            >  2:3891535/1‑150 (MQ=255)
                                                             aGAATGCAGGTAGGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCAAAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAAc                                                                                       >  1:548290/1‑150 (MQ=255)
                                                              gAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACg                                                                                      >  2:340033/1‑150 (MQ=255)
                                                               actGCAGGTATGGGGGGCGGCGCGGGAGCCCGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACgg                                                                                     <  2:2933160/148‑1 (MQ=255)
                                                                 tGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGAt                                                                                   >  1:522290/1‑150 (MQ=255)
                                                                    cgggcgggggggCGGCGCGGGCGCCCGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAACACGGAtttt                                                                                <  2:3002959/144‑1 (MQ=255)
                                                                       tATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGaa                                                                             >  1:3628065/1‑150 (MQ=255)
                                                                       ccggggcggAGGCGCGGCCGGCCGGGGGGCGGCGGCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGaa                                                                             <  2:642707/147‑1 (MQ=255)
                                                                            ggggAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATccgc                                                                        >  2:1972504/1‑150 (MQ=255)
                                                                            ggggAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATccgc                                                                        >  2:2885609/1‑150 (MQ=255)
                                                                                  gcgcGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTc                                                                  >  1:1662841/1‑150 (MQ=255)
                                                                                  gcgcGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTc                                                                  >  1:3038292/1‑150 (MQ=255)
                                                                                    gcgGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTa                                                                >  2:2945311/1‑150 (MQ=255)
                                                                                    gcgGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTa                                                                >  1:733403/1‑150 (MQ=255)
                                                                                    ccgcgactCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTa                                                                <  2:3845289/143‑1 (MQ=255)
                                                                                     cgGGAGCCCGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTAc                                                               <  2:1227681/150‑1 (MQ=255)
                                                                                              ggggggCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGc                                                      >  1:2530457/1‑150 (MQ=255)
                                                                                               gggggCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCACTTCCGcc                                                     >  2:1697961/1‑150 (MQ=255)
                                                                                               gggggCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGcc                                                     >  1:603550/1‑150 (MQ=255)
                                                                                               gggggCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGcc                                                     >  2:1697952/1‑150 (MQ=255)
                                                                                                ggggCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCa                                                    >  1:4419746/1‑150 (MQ=255)
                                                                                                ggggCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCa                                                    >  2:2353697/1‑150 (MQ=255)
                                                                                                 gggCGGCGTCACCGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCTTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCAt                                                   <  2:2748928/150‑1 (MQ=255)
                                                                                                   gcggcgTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGAGTCGAACTCCCACTCCTTTCGTAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCa                                                 >  1:854478/1‑150 (MQ=255)
                                                                                                   gcggcgTCAACGACGTGCGTGAATCGCAGGCAAAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCa                                                 <  1:2898678/150‑1 (MQ=255)
                                                                                                          cAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTg                                          <  2:4145424/150‑1 (MQ=255)
                                                                                                           aaCGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTgg                                         >  2:4271952/1‑150 (MQ=255)
                                                                                                            acgacgTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGgc                                        >  1:2892408/1‑150 (MQ=255)
                                                                                                            acgacgTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGgc                                        >  2:409219/1‑150 (MQ=255)
                                                                                                                    cgtgAATCGCAGGCACAAAAACACCGACCGTACGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGta                                <  2:2806324/150‑1 (MQ=255)
                                                                                                                     gtgCATCGCAGGCACAAAACAACCGTCCGTACGGTCGGTTTTTTCCGGATTGGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGtat                               <  2:4360273/150‑1 (MQ=255)
                                                                                                                        aaTCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATaaa                            <  2:3038292/150‑1 (MQ=255)
                                                                                                                        aaTCGCAGCCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATaaa                            <  2:1977621/150‑1 (MQ=255)
                                                                                                                          tCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAaga                          <  1:1386022/150‑1 (MQ=255)
                                                                                                                           cgctgccacaAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAagag                         <  1:1708378/144‑1 (MQ=255)
                                                                                                                           cGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAagag                         <  2:735045/150‑1 (MQ=255)
                                                                                                                             cAGGCACAAAAAAACCGACCGTACGGTCGGTATTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGt                       <  2:3310227/150‑1 (MQ=255)
                                                                                                                             cAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGt                       <  2:3310224/150‑1 (MQ=255)
                                                                                                                               ggCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGc                     <  1:3891535/150‑1 (MQ=255)
                                                                                                                                gCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGcc                    >  1:785356/1‑150 (MQ=255)
                                                                                                                                 cacaAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCg                   <  1:431125/150‑1 (MQ=255)
                                                                                                                                 caaaaaaaacCCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCg                   <  1:431067/147‑1 (MQ=255)
                                                                                                                                  acaAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGc                  >  2:3330271/1‑150 (MQ=255)
                                                                                                                                  acaAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGc                  <  1:2521881/150‑1 (MQ=255)
                                                                                                                                  acaAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGc                  <  1:263939/150‑1 (MQ=255)
                                                                                                                                    aaaaaaaCCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGCTg                >  1:1511117/1‑150 (MQ=255)
                                                                                                                                    aaaaaaaCCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGCTg                >  2:1984344/1‑150 (MQ=255)
                                                                                                                                    aaaaaaaCCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGCTg                >  1:960631/1‑150 (MQ=255)
                                                                                                                                     aaaaaaCCGACCTTACGGTCGGTTTTTTACGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGGCTACCAATTACGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGCTgg               >  1:1741760/1‑150 (MQ=255)
                                                                                                                                     aaaaaaCCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGCTgg               >  1:1306303/1‑150 (MQ=255)
                                                                                                                                     aaaaaaCCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGCTgg               >  1:3900648/1‑150 (MQ=255)
                                                                                                                                     aaaaaaCCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGCTgg               <  1:319477/150‑1 (MQ=255)
                                                                                                                                     aaaaaaCCGACCGTAAGGTCGGTTTTTTCCGGATTGGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGCTgg               <  2:126601/150‑1 (MQ=255)
                                                                                                                                        aaaCCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGCTggtcg            >  1:3423225/1‑150 (MQ=255)
                                                                                                                                        aaaCCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGCTggtcg            <  2:4142977/150‑1 (MQ=255)
                                                                                                                                         aaCCGACCTTACGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGCTggtc             >  1:2691973/1‑148 (MQ=255)
                                                                                                                                         aaCCGACCTTACGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGCTggtc             <  2:2691973/148‑1 (MQ=255)
                                                                                                                                         aaCCGACCTTACGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACACCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAAATCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGCTggtcgg           >  2:511363/1‑150 (MQ=255)
                                                                                                                                          accgacctTACGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGCTggtcggt          >  2:3377852/1‑150 (MQ=255)
                                                                                                                                          accgacctTACGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGCTggtcggt          >  2:3377830/1‑150 (MQ=255)
                                                                                                                                           ccgaccgTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGCTggtcggtc         >  2:1093702/1‑150 (MQ=255)
                                                                                                                                             gaccgTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGCTGGTCGGTCaa       >  1:14157/1‑150 (MQ=255)
                                                                                                                                             gaccgTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGCTGGTCGGTCaa       >  1:2079352/1‑150 (MQ=255)
                                                                                                                                             gaccgTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGCTGGTCGGTCaa       >  1:4050697/1‑150 (MQ=255)
                                                                                                                                              accgTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGCTGGTCGGTCAAt      <  2:3681131/150‑1 (MQ=255)
                                                                                                                                                cgTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGCTGGTCGGTCAATCg    >  1:2925159/1‑150 (MQ=255)
                                                                                                                                                cgTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGCTGGTCGGTCAATCg    >  1:3505248/1‑150 (MQ=255)
                                                                                                                                                cgTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTAGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGCTGGTCGGTCAATCg    >  1:1882427/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
AATACTTTCGGAATATATCTGACGAATGACAGCTTAAGTGCTAGCGCAAGGGGCGGCCAATAGAATGCAGGTATGGGGGGAGGCGCGGGAGTCAGGGGGGCGGCGTCAACGACGTGCGTGAATCGCAGGCACAAAAAAACCGACCGTAAGGTCGGTTTTTTCCGGATTTGGTGCCCCGAGGGAGACTCGAACTCCCACTCCTTTCGAAAACGGATTTTGAATCCGCCGCGTCTACCAATTCCGCCATCAGGGCGTGGCGCGCAGTATAAAGAGGTGCCGCTGGTCGGTCAATCGGC  >  NC_002947/968733‑969028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑