Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,011,483 T→A 5.2% intergenic (‑418/‑109) PP_0871 ← / → prfC glycine betaine/carnitine/choline ABC transporter permease/peptide chain release factor 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,011,4830TA 5.2% 596.5 / 13.5 188intergenic (‑418/‑109)PP_0871/prfCglycine betaine/carnitine/choline ABC transporter permease/peptide chain release factor 3
Reads supporting (aligned to +/- strand):  ref base T (102/74);  new base A (7/3);  total (109/79)
Fisher's exact test for biased strand distribution p-value = 5.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 190 total aligned reads displayed.

TTGAAATAACTGCGTTTTGGTTATTTGTCGTGATAAAGCGATTGCCTGTTGGGTTTGTTTTGTTTGTGCCGGCCTCTTCGCGGGTAAACTCGCTCCTACGAAGACTGCACCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATCCCCCAGTCAGGCGATTTCCCATGACCAACCAGGCCGCCGAAGTCGCGAAGCGCCGCACTT  >  NC_002947/1011335‑1011631
                                                                                                                                                    |                                                                                                                                                    
ttGAAATAACTGCGTTTTGGTTATTTGTCGTGATAAAGCGATTGCCTGTTGGGTTAGTTTTGTTTGTGCCGTCCTCTTCGCGGGTAAACTCGCTCCTACGAAGACTGCACCGCATCCGAACCCTGTGTCGTAACTGTAGGCGCGGGTTTa                                                                                                                                                     >  2:3722621/1‑150 (MQ=255)
 tGAAATAACTGCGTTTTGGTTATTTGTCGTGATAAAGCGATTGCCTGTTGGGTTTGTTTTGTTTGTGCCGGCCTCTTCGCGGGTAAACTCGCTCCTACGAAGACTGCACCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTAc                                                                                                                                                    <  2:2002711/150‑1 (MQ=255)
 tGAAATAACTGCGTTTTGGTTATTTGTCGTGATAAAGCGATTGCCTGTTGGGTTTGTTTTGTTTGTGCCGGCCTCTTCGCGGGTAAACTCGCTCCTACGAAGACTGCACCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTAc                                                                                                                                                    <  1:1219074/150‑1 (MQ=255)
 tGAAATAACTGCGTTTTGGTTATTTGTCGTGATAAAGCGATTGCCTGTTGGGTTTGTTTTGTTTGTGCCGGCCTCTTCGCGGGTAAACTCGCTCCTACGAAGACTGCACCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTAc                                                                                                                                                    <  2:2985031/150‑1 (MQ=255)
  gAAATAACTGCGTTTTGGTTATTTGTCGTGATAAAGCGATTGCCTGTTGGGTTTGTTTTGTTTGTGCCGGCCTCTTCGCGGGTAAACTCGCTCCTACGAAGACTGCACCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTAcc                                                                                                                                                   >  2:3938006/1‑150 (MQ=255)
   aaaTAACTGCGTTTTGGTTATTTGTCGTGATAAAGCGATTGCCTGTTGGGTTTGTTTTGTTTGTGCCGGCCTCTTCGCGGGTAAACTCGCTCCTACGAAGACTGCACCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTAccc                                                                                                                                                  >  2:4107113/1‑150 (MQ=255)
   aaaTAACTGCGTTTTGGTTATTTGTCGTGATAAAGCGATTGCCTGTTGGGTTTGTTTTGTTTGTGCCGGCCTCTTCGCGGGTAAACTCGCTCCTACGAAGACTGCACCGCATACGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTAccc                                                                                                                                                  >  1:3593068/1‑150 (MQ=255)
        aCTGCGTTTTGGTTATTTGTCGTGATAAAGCGATTGCCTGTTGGGTTTGTTTTGTTTGTGCCGGCCTCTTCGCGGGTAAACTCGCTCCTACGAAGACTGCACCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGaa                                                                                                                                             <  2:1426434/150‑1 (MQ=255)
        aCTGCGTTTTGGTTATTTGTCGTGATAAAGCGATTGCCTGTTGGGTTTGTTTTGTTTGTGCCGGCCTCTTCGCGGGTAAACTCGCTCCTACGAAGACTGCACCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGaa                                                                                                                                             >  1:743117/1‑150 (MQ=255)
          tGCGTTTTGGTTATTTGTCGTGATAAAGCGATTGCCTGTTGGGTTTGTTTTGTTTGTGCCGGCCTCTTCGCGGGTAAACACGCTCCTACGAAGACTGCACCGCATCCGAACCCTGTGTCGTACCTGTATGCGCGGGTTTACCCGCGAAgg                                                                                                                                           >  1:3441383/1‑150 (MQ=255)
               tttGGTTATTTGTCGTGATAAAGCGATTGCCTGTTGGGTTTGTTTTGTTTGTGCCGGCCTCTTCGCGGGTAAACTCGCTCCTACGAAGACTGCACCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCg                                                                                                                                      <  2:2700246/150‑1 (MQ=255)
                ttGGTTATTTGTCGTGATAAAGCGATTGCCTGTTGGGTTTGTTTTGTTTGTGCCGGCCTCTTCGCGGGTAAACTCGCTCCTACGAAGACTGCACCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCgg                                                                                                                                     <  2:1008589/150‑1 (MQ=255)
                    ttATTTGTCGTGATAAAGCGATTGCCTGTTGGGTTTGTTTTGTTTGTGCCGGCCTCTTCGCGGGTAAACTCGCTCCTACGAAGACTGCACCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGcaca                                                                                                                                 >  1:4196098/1‑150 (MQ=255)
                    ttATTTGTCGTGATAAAGCGATTGCCTGTTGGGTTTGTTTTGTTTGTGCCGGCCTCTTCGCGGGTAAACTCGCTCCTACGAAGACTGCACCGCATCCGAACCCAGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGccca                                                                                                                                 >  1:107448/1‑150 (MQ=255)
                    ttATTTGTCGTGATAAAGCGATTGCCTGTTGGGTTTGTTGGGTTTGTGCCGGCCTCTTCGCGGGTAAACTCGCTCCTACGAAGACTGCACCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGcaca                                                                                                                                 <  2:2405589/150‑1 (MQ=255)
                              tGATAAAGCGATTGCCTGTTGGGTTTGTTTTGTTTGTGCCGGCCTCTTCGCGGGTAAACTCGCTCCTACGAAGACTGCACCGCATCCGAACCCAGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACACGCGCcaatc                                                                                                                       >  1:3887587/1‑150 (MQ=255)
                                  aaaGCGATTGCCTGTTGGGTTTGTTTTGTTTGTGCCGGCCTCTTCGCGGGTAAACTCGCTCCTACGAAGACTGCACCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCattcattc                                                                                                                   >  2:188173/1‑150 (MQ=255)
                                  aaaGCGATTGCCTGGTGGGTTTGTTTTGTTTGTGCCGGCCTCTTCGCGGGTAAACTCGCTCCTACGAAGACTGCACCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCcttcattc                                                                                                                   >  2:1927479/1‑150 (MQ=255)
                                      cGATTGCCTGTTGGGTTTGTTTTGTTTGTGCCGGCCTCTTCGCGGGTAAACTCGCTCCTACGAAGACTGCACCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCa                                                                                                               >  2:10056/1‑150 (MQ=255)
                                         ttGCCTGTTGGGTTTGTTTTGTTTGTGCCGGCCTCTTCGCGGGTAAACACGCTCCTACGAAGACTGCACCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCAATCATTCGGCAGAt                                                                                                            >  2:3195187/1‑150 (MQ=255)
                                           gCCTGTTGGGTTTGTTTTGTTTGTGCCGGCCTCTTCGCGGGTAAACTCGCTCCTACGAAGACTGCACCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCAATCATTAGGCAGAttt                                                                                                          >  1:3022621/1‑150 (MQ=255)
                                             gtGTTGGGTTTGTTTTGTTTGTGCCGGCCTCTTCGCGGGTAAACTCGCTCCTACGATGCCTGCACCGCATCCGAACCCTGTGTGGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTg                                                                                                        <  1:3310018/149‑1 (MQ=255)
                                             cTGTTGGGTTTGTTTTGTTTGTGCCGGCCTCTTCGCGGGTAAACTCGCTCCTACGAAGACTGCACCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTg                                                                                                        <  2:3064408/150‑1 (MQ=255)
                                              tGTTGGGTTTGTTTTGTTTGTGCCGGCCTCTTCGCGGGTAAACTCGCTCCTACGAAGACTGCACCGCATCCGAACCCTCTGTCGTACCTGTAGGCGCGGGTCTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTgg                                                                                                       >  2:3993627/1‑150 (MQ=255)
                                                   ggTTTGTTTTGTTTGTGCCGGCCTCTTCGCGGGTAAACTCGCTCCTACGAAGACTGCACCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAg                                                                                                  <  2:3713918/150‑1 (MQ=255)
                                                   ggTTTGTTTTGTTTGTGCCGGCCTCTTCGCGGGTAAACTCGCTCCTACGAAGACTGCACCGCATCCGAACCCTGTGTCGTACCGGGAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAg                                                                                                  <  1:2584868/150‑1 (MQ=255)
                                                    gtttgtttTGTTTGTGACGGCCTCTTCGCGGGTAAACTCGCTCCTACGAAGACTGCACCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACACGCGCCATTCAATCGGCAGATATTGGTCCAgg                                                                                                 >  2:2866438/1‑150 (MQ=255)
                                                       tgttttgtttGTGCCGGCCTCTTCGCGGGTAAACTCGCTCCTACGAAGACTGCACCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGccc                                                                                              <  2:4253117/150‑1 (MQ=255)
                                                        gttttgtttGTGCCGGCCTCTTCGCGGGTAAACTCGCTCCTACGAAGACTGCACCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCa                                                                                             >  1:3994411/1‑150 (MQ=255)
                                                            tgtttgtGCCGGCCTCTTCGCGGGTAAACTCGCTCCTACGAAGACTGCACCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACtt                                                                                         >  2:1915141/1‑150 (MQ=255)
                                                               ttgtGCCGGCCTCTTCGCGGGTAAACTCGCTCCTACGAAGACTGCACCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAg                                                                                      >  2:4300538/1‑150 (MQ=255)
                                                               ttgtGCCGGCCTCTTCGCGGGTAAACTCGCTCCTACGAAGACTGCACCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAg                                                                                      <  2:2848598/150‑1 (MQ=255)
                                                                          tctTCGCGGGTAAACTCGCTCCTACGAAGACTGCACCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTg                                                                           >  1:2338018/1‑150 (MQ=255)
                                                                          tctTCGCGGGTAAACTCGCTCCTACGAAGACTGCACCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTg                                                                           <  2:3467815/150‑1 (MQ=255)
                                                                              cgcgGGTAAACTCGCTCCTACGAAGACTGCACCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGcccc                                                                       <  1:2228860/150‑1 (MQ=255)
                                                                                 gggTAAACTCGCTCCTACGAAGACTGCACCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCttt                                                                    <  2:2480856/150‑1 (MQ=255)
                                                                                 gggTAAACTCGCTCCTACGAAGACTGCACCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCttt                                                                    >  2:744425/1‑150 (MQ=255)
                                                                                   gTAAACTCGCTCCTACGAAGACTGCACCGCATCCGAACCCTGTGTCGTACCTGTCGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCa                                                                  <  1:1033863/150‑1 (MQ=255)
                                                                                   gTAAACTCGCTCCTACGAAGACTGCACCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCa                                                                  <  1:3659330/150‑1 (MQ=255)
                                                                                   gTAAACTCGCTCATACGAACACTGCACCGCATCCGAACCGTGTGTCGTACCTGTCGGCGCGGGTTGACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCa                                                                  <  1:794700/150‑1 (MQ=255)
                                                                                     aaaaTCGCTCCTCCGTTGACTGCACGGCTTCCGAACCCTGTGTGGTACATGTGGGGGCGTTTTAACCCGCGATGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCaaa                                                                <  1:2107077/150‑1 (MQ=255)
                                                                                       aCTCGCTCCTACGAAGACTGCACCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATc                                                              >  1:2321200/1‑150 (MQ=255)
                                                                                       aCTCGCTCCTACGAAGACTGCACCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATc                                                              >  2:2352964/1‑150 (MQ=255)
                                                                                        cgcGCTCCTACTAAGACTGCACCGCCTCCGAACCCTGTGTCGTACCTGGCGGCGCGGGTTTCCCCGCGATGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATcc                                                             <  1:2795156/148‑1 (MQ=255)
                                                                                        cTCGCTCCTACGAAGACTGCACCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATcc                                                             >  1:3288311/1‑150 (MQ=255)
                                                                                         tCGCTCCTACGAAGACTGCACCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATccc                                                            >  2:528646/1‑150 (MQ=255)
                                                                                         tCGCTCCTACGAAGACTGCACCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATccc                                                            >  1:2660914/1‑150 (MQ=255)
                                                                                         tCGCTCCTACGAAGACTGCACCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATccc                                                            >  1:1978454/1‑150 (MQ=255)
                                                                                          cGCTCCTACGAAGACTGCACCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATcccc                                                           <  2:4100592/150‑1 (MQ=255)
                                                                                            cTCCTACGAAGACTGCACCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATCCCCCa                                                         <  2:1320781/150‑1 (MQ=255)
                                                                                            cTCCTACGAAGACTGCACCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATCCCCCa                                                         <  1:1748973/150‑1 (MQ=255)
                                                                                             tCCTACGAAGACTGCACCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATCCCCCAg                                                        >  1:666039/1‑150 (MQ=255)
                                                                                              ccTACGAAGACTGCACCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATCCCCCAGt                                                       <  2:254956/150‑1 (MQ=255)
                                                                                               cTACGAAGACTGCACCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTATCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATCCCCCAGTc                                                      >  1:3275823/1‑150 (MQ=255)
                                                                                               cTACGAAGACTGCACCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATCCCCCAGTc                                                      <  2:2813049/150‑1 (MQ=255)
                                                                                                  cGAAGACTGCACCGCATCCTCACCGTGTGTCGTACCTGTTGGCGGGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATCCCCCAGTCAgg                                                   <  1:1196168/150‑1 (MQ=255)
                                                                                                  cGAAGACTGCACCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATCCCCCAGTCAgg                                                   <  2:3894237/150‑1 (MQ=255)
                                                                                                      gACTGCACCGCATCCGACCCCTGTGTCGTACCTGCAGGCGCGGGTTTACCCGCGACGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATCCCCCAGTCAGGCGAt                                               <  1:2461493/150‑1 (MQ=255)
                                                                                                      gACTGCACCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATCCCCCAGTCAGGCGAt                                               >  1:2887726/1‑150 (MQ=255)
                                                                                                         tGCACCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATCCCCCAGTCAGGCGATTTc                                            <  1:4449667/150‑1 (MQ=255)
                                                                                                         tGCACCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATCCCCCAGTCAGGCGATTTc                                            >  1:1975302/1‑150 (MQ=255)
                                                                                                         tGCACCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATCCCCCAGTCAGGCGATTTc                                            >  1:4500187/1‑150 (MQ=255)
                                                                                                            aCCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATCCCCCAGTCAGGCGATTTCCCa                                         <  2:754640/150‑1 (MQ=255)
                                                                                                            aCCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATCCCCCAGTCAGGCGATTTCCCa                                         >  2:3509009/1‑150 (MQ=255)
                                                                                                            aCCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATCCCCCAGTCAGGCGATTTCCCa                                         <  2:1574330/150‑1 (MQ=255)
                                                                                                            aCCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATCCCCCAGTCAGGCGATTTCCCa                                         >  1:597214/1‑150 (MQ=255)
                                                                                                             ccGCATCCGAACCCTGTGTCGTCCCTGTCGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATCCCCCAGTCAGGCGATTTCCCAt                                        <  1:3550690/150‑1 (MQ=255)
                                                                                                             ccGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATCCCCCAGTCAGGCGATTTCCCAt                                        <  1:365397/150‑1 (MQ=255)
                                                                                                              cGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATCCCCCAGTCAGGCGATTTCCCATg                                       >  1:1911893/1‑150 (MQ=255)
                                                                                                               gCATCCGAACCGTGTGTCGTACCTGTCGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATCCCCCAGTCAGGCGATTTCCCATGa                                      <  1:909606/150‑1 (MQ=255)
                                                                                                               gCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATCCCCCAGTCAGGCGATTTCCCATGa                                      <  2:1716131/150‑1 (MQ=255)
                                                                                                                     gAACCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATCCCCCTGTCAGGCGATTTCCCATGaccaacc                                >  2:1150576/1‑150 (MQ=255)
                                                                                                                       aCCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATCCCCCAGTCAGGCGATTTCCCATGACCAACCAg                              <  2:2166357/150‑1 (MQ=255)
                                                                                                                       aCCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATCCCCCAGTCAGGCGATTTCCCATGACCAACCAg                              >  1:3873578/1‑150 (MQ=255)
                                                                                                                       aCCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATCCCCCAGTCAGGCGATTTCCCATGACCAACAAg                              >  1:1598098/1‑150 (MQ=255)
                                                                                                                          cTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATCCCCCAGTCAGGCGATTTCCCATGACCAACCAGgcc                           >  1:3879580/1‑150 (MQ=255)
                                                                                                                             tgtCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCgg                                                                                                                 >  1:1086562/1‑61 (MQ=255)
                                                                                                                                 gTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATCCCCCAGTCAGGCGATTTCCCATGACCAACCAGGCCGCCGAAg                    >  2:1270642/1‑150 (MQ=255)
                                                                                                                                  tcccTGTTGGCGCGGGTCTCCCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATCCCCCAGTCAGGCGATTTCCCATGACCAACCAGGCCGCCGAAGt                   <  1:3179779/148‑1 (MQ=255)
                                                                                                                                  tACCTGTTGGCGCGTTTTTACCCGCGCAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATCCCCCAGTCAGGCGATTTCCCATGACCAACCAGGCCGCCGAAGt                   <  1:2412667/150‑1 (MQ=255)
                                                                                                                                   aCCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATCCCCCAGTCAGGCGATTTCCCATGACCAACCAGGCCGCCGAAGTc                  >  1:1169033/1‑150 (MQ=255)
                                                                                                                                   aCCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATCCCCCAGTCAGGCGATTTCCCATGACCAACCAGGCCGCCGAAGTc                  >  2:2256377/1‑150 (MQ=255)
                                                                                                                                     cTGTCGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATCCCCCAGTCAGGCGATTTCCCATGACCAACCAGGCCGCCGAAGTcgc                <  1:3389395/150‑1 (MQ=255)
                                                                                                                                     cTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATCCCCCAGTCAGGAGATTTCCCATGACCAACCAGCCCGCCGAAGTcgc                >  1:1319134/1‑150 (MQ=255)
                                                                                                                                      tGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATCCCCCAGTCAGGCGATTTCCCATGACCAACCAGGCCGCCGAAGTcgcg               <  2:1686653/150‑1 (MQ=255)
                                                                                                                                       gTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATCCCCCAGTCAGGCGATTTCCCATGACCAACCAGGCCGCCGAAGTCGCGa              >  2:3960630/1‑150 (MQ=255)
                                                                                                                                       gTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATCCCCCAGTCAGGCGATTTCCCATGACCAACCAGGCCGCCGAAGTCGCGa              >  2:3960571/1‑150 (MQ=255)
                                                                                                                                        tAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATCCCCCAGTCAGGCGATTTCCCATGACCAACCAGGCCGCCGAAGTCGCGaa             >  2:2931680/1‑150 (MQ=255)
                                                                                                                                        ccttcgcgGGTAAACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTACAAATCCCCAAGTCAGGCGATTTCCCATGACCAACCAGGCCGCAGAAGTAGCTaa             >  2:197757/5‑150 (MQ=255)
                                                                                                                                         cttcgcgGGTAAACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATCCCCCAGTCAGGCGATTTCCCATGACCAACCAGGCCGCCGAAGTCGCGAAg            >  1:2823911/4‑150 (MQ=255)
                                                                                                                                         cttcgcgGGTAAACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATCCCCCAGTCAGGCGATTTCCCATGACCAACCAGGCCGCCGAAGTCGCGAAg            >  1:2706359/4‑150 (MQ=255)
                                                                                                                                         cttcgcgGGTAAACCCGCGAAGGGGCCGGCACAGGCGCCATTAATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGTTATGATACTGCCCCTTTCAAATCCCCCAGTCAGGCGATTTCCCATGACCAACCAGGCCGCCGAAGTCGCGAAg            >  1:2706239/4‑150 (MQ=255)
                                                                                                                                           tcgcgGGTAAACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATCCCCCa                                                         >  1:842709/2‑103 (MQ=255)
                                                                                                                                             gcgGGTTCCCCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATCCCCCAGTCAGGCGATTTCCCATGACCAACCAGGCCGCCGAAGTCGCGAAGcgcc        <  1:3790676/150‑1 (MQ=255)
                                                                                                                                              cgGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATCCCCCAGTCAGGCGATTTCCCATGACCAACCAGGCCGCCGAAGTCGCGAAGcgccg       <  1:3482771/150‑1 (MQ=255)
                                                                                                                                              cgGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATAATGCCCCTTTCAAATCCCCCAGTCAGGCGATTTCCCATGACCAACCAGGCCGCCGAAGTCGCGAAGcgccg       <  2:3014077/150‑1 (MQ=255)
                                                                                                                                               gggTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATCCCCCAGTCAGGCGATTTCCCATGACCAACCAGGCCGCCGAAGTCGCGAAGcgccgc      >  2:2263632/1‑150 (MQ=255)
                                                                                                                                                 gTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATCCCCCAGTCAGGCGATTTCCCATGACCAACCAGGCCGCCGAAGTCGCGAAGCGCCGCAc    >  1:2730110/1‑150 (MQ=255)
                                                                                                                                                 gTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATCCCCCAGTCAGGCGATTTCCCATGACCAACCAGGCCGCCGAAGTCGCGAAGCGCCGCAc    >  1:854033/1‑150 (MQ=255)
                                                                                                                                                   ttACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATCCCCCAGTCAGGCGATTTCCCATGACCAACCAGGCCGCCGAAGTCGCGAAGCGCCGCACtt  >  2:2652583/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TTGAAATAACTGCGTTTTGGTTATTTGTCGTGATAAAGCGATTGCCTGTTGGGTTTGTTTTGTTTGTGCCGGCCTCTTCGCGGGTAAACTCGCTCCTACGAAGACTGCACCGCATCCGAACCCTGTGTCGTACCTGTAGGCGCGGGTTTACCCGCGAAGGGGCCGGCACAGGCGCCATTCATTCGGCAGATTTTGGTCCAGGCCCAACTTCAGGTATGATACTGCCCCTTTCAAATCCCCCAGTCAGGCGATTTCCCATGACCAACCAGGCCGCCGAAGTCGCGAAGCGCCGCACTT  >  NC_002947/1011335‑1011631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑