Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 170,841 A→C 7.9% intergenic (+22/‑548) PP_0159 → / → PP_16SA family III CoA‑transferase/16S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947170,8410AC 7.9% 408.6 / 15.2 153intergenic (+22/‑548)PP_0159/PP_16SAfamily III CoA‑transferase/16S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (61/74);  new base C (7/7);  total (68/85)
Fisher's exact test for biased strand distribution p-value = 7.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 155 total aligned reads displayed.

CGGAGACGCCAGTGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGTTAAGTAGTTAAGGCA  >  NC_002947/170692‑170988
                                                                                                                                                     |                                                                                                                                                   
cGGAGACGCCAGTGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCa                                                                                                                                                     <  2:646969/150‑1 (MQ=255)
  gagaCGCCAGTGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGGGGCCGTCAgg                                                                                                                                                   >  1:1514474/1‑150 (MQ=255)
  gagaCGCCAGTGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGCGGCCGTCAgg                                                                                                                                                   >  1:1514270/1‑150 (MQ=255)
   agaCGCCAGTGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGc                                                                                                                                                  <  2:725784/150‑1 (MQ=255)
   agaCGCCAGTGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGc                                                                                                                                                  <  2:3620554/150‑1 (MQ=255)
   agaCGCCAGTGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGc                                                                                                                                                  <  2:3363666/150‑1 (MQ=255)
    gaCGCCAGTGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGTGGCCGTCAGGcg                                                                                                                                                 >  2:3920332/1‑149 (MQ=255)
       gCCAGTGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTcc                                                                                                                                              <  2:4314076/150‑1 (MQ=255)
       gCCAGTGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTcc                                                                                                                                              <  2:2388894/150‑1 (MQ=255)
        ccAGTGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTccc                                                                                                                                             >  1:3879957/1‑150 (MQ=255)
         cAGTGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCTGACGGCCTcccc                                                                                                                                            <  1:2681711/150‑1 (MQ=255)
         cAGTGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTcccc                                                                                                                                            >  2:3788856/1‑150 (MQ=255)
           gTGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCTGACGGCCTCCCCGc                                                                                                                                          <  2:3475195/150‑1 (MQ=255)
           gTGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGc                                                                                                                                          <  2:3739668/150‑1 (MQ=255)
             tgAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGATGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGTGGGGAGGCCTGACGGCCTCCCCGCtt                                                                                                                                        <  2:1810048/149‑1 (MQ=255)
             ggAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGTGGGGAGGCCTGACGGCCTCCCCGCtt                                                                                                                                        <  2:1709196/150‑1 (MQ=255)
                 tATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGtt                                                                                                                                    >  2:1085032/1‑150 (MQ=255)
                    cGCCGGGCGCCACCAGTGCTGGGCGAGCATAGCGAAGTGGTGCTGGGGGATGTGTTGGGGCTTGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCCCCCCGCTTTGttttt                                                                                                                                 <  1:3248409/150‑1 (MQ=255)
                      ccGGGCGCCACCACTGCTGGGCGAGCATACCGACGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCa                                                                                                                               <  2:2460581/150‑1 (MQ=255)
                        gggCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAgc                                                                                                                             <  2:987057/150‑1 (MQ=255)
                            gcCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGAGGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTa                                                                                                                         <  2:3175455/150‑1 (MQ=255)
                               accacTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCCGCGCTATCa                                                                                                                      >  2:2828673/1‑150 (MQ=255)
                               accacTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCa                                                                                                                      >  2:3188904/1‑150 (MQ=255)
                                ccacTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCCGCGCTATCaa                                                                                                                     >  2:578986/1‑150 (MQ=255)
                                 cacgggtgGGCGCGCATACCTCAGTGTGTCTGGGGGTTGTGTTGGGGCTGGTTGGTGGCGTGGTACCGGGTCGGCGTGATGGTGGGGTGCTCTATTTGAAACGGGTGGTGGCCTTCTGGCCTCCCCGCTTTGTTTTTCAGCGCTATCaaa                                                                                                                    <  1:3638144/144‑1 (MQ=255)
                                 cacTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCaaa                                                                                                                    >  1:850794/1‑150 (MQ=255)
                                   ggggggggCGCGCATACGGTGGGGGTGGGGGGGGAGGTGTGGGGGGGGGTTGCGGGGGTGGTACCGGGTCTGGGTGATGGGGGGGTGCTCTAGATGAAAGGGGGGGCGGCCGTCCGGCCCCCCCGCTTTGTTTTTCAGCGCTATCAAAgg                                                                                                                  <  1:2133487/145‑1 (MQ=255)
                                    tgctgGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGGCAGGCCTCCCCGCGTTGTTTTTGAGCGCTATAAAAGGt                                                                                                                 >  1:1997755/1‑150 (MQ=255)
                                      ctgGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCGGTGTTTTTCAGCGCTATCAAAGGTaa                                                                                                               >  2:4200935/1‑150 (MQ=255)
                                      ctgGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCGCTAGATGAAAAGCGGGGGGGCCGGCCGGCCGCCCCGCGTTGGTTTTCAGCGCTATCAAAGGtca                                                                                                               >  1:2405410/1‑148 (MQ=255)
                                        gggCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCGTTGTTTTTCAGCGCTATCAAAGGTAAtt                                                                                                             >  2:1828846/1‑150 (MQ=255)
                                            gAGCATACCGAAGTGGTGCTGGGGGAATTGTTGGGGCTGGATGCTGGCGAGGTAGAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGaaa                                                                                                         <  2:4119585/150‑1 (MQ=255)
                                            gAGCATACCGAAGTGGTGCGGGGGGATGTGTTGGGGCTGGATGCTGGCGTGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGTGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGaaa                                                                                                         <  2:3800856/150‑1 (MQ=255)
                                                 tACCGTAGTGGTGCTGGGGGATGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCATTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTaaa                                                                                                    <  2:2402788/150‑1 (MQ=255)
                                                 tACCGAAGTGGTGCTGGGGGATGTGTTGGGGGTGGATGCTGGCGAGTTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGCCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTaaa                                                                                                    <  2:3781231/150‑1 (MQ=255)
                                                 tACCGAAGTGGTGCTGGGGGATGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTaaa                                                                                                    <  2:3216016/150‑1 (MQ=255)
                                                 tACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGGGTGATGCTGGGGTGCTCTTGATGAAAAGCGGGGCGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTaaa                                                                                                    <  1:4425479/150‑1 (MQ=255)
                                                 tACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTaaa                                                                                                    <  2:479444/150‑1 (MQ=255)
                                                  agcGAAGTGGTGCTGGGGGTTGTGTTGGGGGTGGATGCTGGCGAGGTACTGCGTCTGCGTGTTGCTGGGGTGCTCTTGATGAAAAGCGGGGAGGCCGTCAGGGCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAg                                                                                                   <  1:3346747/148‑1 (MQ=255)
                                                  aCCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAg                                                                                                   <  2:2945720/150‑1 (MQ=255)
                                                  aCCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCGCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAg                                                                                                   >  1:2895570/1‑150 (MQ=255)
                                                      aaGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAATGTAATTTAAATTAAAGGttt                                                                                               >  2:894417/1‑149 (MQ=255)
                                                        gtggtgCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGAc                                                                                             <  2:3557161/150‑1 (MQ=255)
                                                              cTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCctc                                                                                       >  2:3916364/1‑150 (MQ=255)
                                                               tGGGGGATGTGTTGGGGCTGGAGGGTGGGGTGGTACAGGGTCTGGGTGTTGGTGGGGTGCTCTAGATGAAAAGCGGGGCGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCctct                                                                                      <  1:2440671/150‑1 (MQ=255)
                                                               tGGGGGAAGTGTTGGGGCTGGATGCTGGCGTGGTACCGCGTCTGCGTGTTGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCctct                                                                                      <  2:1502919/150‑1 (MQ=255)
                                                                gggggAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCtctc                                                                                     <  2:1428024/150‑1 (MQ=255)
                                                                gggggAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCtctc                                                                                     <  2:2609393/150‑1 (MQ=255)
                                                                    gAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAAt                                                                                 >  2:832612/1‑150 (MQ=255)
                                                                        tgtTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATcccc                                                                             <  2:3294293/150‑1 (MQ=255)
                                                                         gtTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCGATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCt                                                                            >  1:2607393/1‑150 (MQ=255)
                                                                          ttGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGGGGCCGTCACGCCTCCCCGCGTTGTTTTTAAGCGCTATCAAATGTAAGTGTAATTAAATGTTGTCGCCCACTCGAATacccgg                                                                           >  1:122850/1‑148 (MQ=255)
                                                                          ttGGGGCTGGATGCCGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCtt                                                                           <  2:2580320/150‑1 (MQ=255)
                                                                              ggCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGATTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATaa                                                                       <  2:2514918/150‑1 (MQ=255)
                                                                                cTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCCGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCGTATAATg                                                                     >  1:2212300/1‑150 (MQ=255)
                                                                                cTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCCGCGCTATCAAAGGTAATTGAAATTAAAGGTTGAAGCCCTCTCGAATCCCCGGATAATg                                                                     >  2:4158443/1‑150 (MQ=255)
                                                                                 tGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCGTTGTTTTTCCGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGCATCACCGTATAATgc                                                                    >  2:3134440/1‑150 (MQ=255)
                                                                                 gggATGGTGGCGTGTTACAGGGTGTGCGTGATGGTGGGGTGCTATATATTAAATGGGGGGCGGCCGTCAGCCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATgc                                                                    <  1:580146/149‑1 (MQ=25)
                                                                                  ggATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTTTTTTTAAGCGCTATCAAAGGTAATTTAAATTAAAGTTTTTCGCCCTCTCGAATACCCATATAATtcg                                                                   >  1:2121400/1‑150 (MQ=255)
                                                                                   gATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATgcgc                                                                  <  1:4516090/150‑1 (MQ=255)
                                                                                     tGCTGGCGCGGTACCGCGTCTGCGTGATGCTGGGGTGCTCTAGATCAAACGCGGGGAGGCCGTCAGGCCCCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGccc                                                                <  1:3175106/150‑1 (MQ=38)
                                                                                      gCTGGCGTGGTACAGGGTCTGCGTGATGGTGGGGTGCTCTAGATGAAACGCGGGGAGGCCGTCTGGCCCCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGcccc                                                               <  1:2107468/150‑1 (MQ=38)
                                                                                        tGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGCGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATAAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATACCCGTATAATGCGCCCCAc                                                             >  1:3583204/1‑150 (MQ=255)
                                                                                        tGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTGATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCAc                                                             >  2:4496229/1‑150 (MQ=255)
                                                                                        tGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCAc                                                             >  2:2705079/1‑150 (MQ=255)
                                                                                        tGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAAATGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCAc                                                             >  2:4090515/1‑150 (MQ=255)
                                                                                        tGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGGCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCAc                                                             >  2:2705190/1‑150 (MQ=255)
                                                                                               gTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGc                                                      >  2:1460743/1‑150 (MQ=255)
                                                                                                     cGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATc                                                <  2:208221/150‑1 (MQ=255)
                                                                                                     cGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATc                                                <  2:2465980/150‑1 (MQ=255)
                                                                                                        cTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGa                                             <  2:3897117/150‑1 (MQ=255)
                                                                                                         tGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGaa                                            <  1:3223825/150‑1 (MQ=255)
                                                                                                           cGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCACCCCGCGGTGTTTTTCAGCGCGATCAAAGGTAATTGAAAATAAAGGTTGACCCCCTCTCGAATCCCCTTATAAAGCGCCCCACTTACAGCCACATCCGAACg                                          >  2:1733329/1‑150 (MQ=37)
                                                                                                                tGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGGCTCCCCGGTTTGTTTTTGAGGGCTATCAAAGGTAATTGAAATTAAATGTTGTCGCCCTCTCGAATACCCTTATAATGCGCCCCACTTCCAGCGAAATAGGAAAGaaaaa                                     >  1:4519190/1‑150 (MQ=25)
                                                                                                                tGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGaaaaa                                     <  1:3711342/150‑1 (MQ=255)
                                                                                                                tGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAACTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGaaaaa                                     <  1:3711330/150‑1 (MQ=255)
                                                                                                                   tGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGCTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGAAGCCCACTCGAATCCCCTTATAAATCCCCCCACTTCCAGCGACAACGGAACGAAAAACTc                                  >  1:2626305/1‑150 (MQ=37)
                                                                                                                    ggggTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTcc                                 <  1:4450522/150‑1 (MQ=255)
                                                                                                                    ggggTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTcc                                 <  1:1460743/150‑1 (MQ=255)
                                                                                                                     gggTGCTCTAGTTGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCt                                <  2:1431289/150‑1 (MQ=255)
                                                                                                                      gttGCTATATATTAAATGCGGGGAGGCGGTCTGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCtt                               <  1:1457367/148‑1 (MQ=21)
                                                                                                                      ggTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCtt                               <  2:1535962/150‑1 (MQ=255)
                                                                                                                           tctAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCAATTCCAGCGACATCGGAACGAAAAACTCCTTGAGAt                          >  2:2179560/1‑150 (MQ=255)
                                                                                                                           tctAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTAAGCGCTATAAAAGGTAATTGAAATTAAAGGTTGAAGCCCAAACGAATCCCCTTATAATGCGCCCCACTTACAGCGACAACGGAACGAAAAACTCCATGAGAt                          >  1:1861683/1‑150 (MQ=25)
                                                                                                                           tctAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCCCTTTGTTTTTCAGCGCTATAAAAGGTAATTGAAATTAAATGTTGACGCCCACTCGAATACCCTTATAAAGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTACATGAGAt                          >  1:2584700/1‑150 (MQ=25)
                                                                                                                             ttgATTAAACGGGGGGAGGCCGTCAGGCCGCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTc                        <  1:3788898/148‑1 (MQ=255)
                                                                                                                             tAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATTCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTc                        >  2:4411571/1‑150 (MQ=255)
                                                                                                                               gATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCaa                      <  2:1241922/150‑1 (MQ=255)
                                                                                                                               aaaacaaaGCGGGGAGGGCTGACGGCCTCCCCGCTTTGTTTTTCAGGGCTATGAAATGTAATTTTAATTAAAGGTTTACGCCCTCTCGAATACCCTTATAATGGGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCaa                      >  2:864066/6‑150 (MQ=17)
                                                                                                                                  gAAAAGCGGGGCGGCCGTCAGGCCGCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGa                   <  1:4233259/150‑1 (MQ=255)
                                                                                                                                  gAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGa                   <  2:3660709/150‑1 (MQ=255)
                                                                                                                                    aaaGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGt                 <  1:1085032/150‑1 (MQ=255)
                                                                                                                                        cGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCAATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGTTAag             >  1:571441/1‑150 (MQ=255)
                                                                                                                                         ggggAGGCCTGACGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGTTAagt            >  2:3887767/1‑150 (MQ=255)
                                                                                                                                          gggAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGTTAagta           <  1:3220848/150‑1 (MQ=255)
                                                                                                                                           ggAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGTTAagtag          >  1:2485066/1‑150 (MQ=255)
                                                                                                                                             aGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGTTAAGTAGtt        <  2:95115/150‑1 (MQ=255)
                                                                                                                                               gCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGTTAAGTAGTTaa      >  2:1839197/1‑150 (MQ=255)
                                                                                                                                                 cGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGTTAAGTAGTTAAgg    <  2:1514474/150‑1 (MQ=255)
                                                                                                                                                 cGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGTTAAGTAGTTAAgg    >  1:2390138/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CGGAGACGCCAGTGGAGTATCGCCGGGCGCCACCACTGCTGGGCGAGCATACCGAAGTGGTGCTGGGGGAAGTGTTGGGGCTGGATGCTGGCGAGGTACAGCGTCTGCGTGATGCTGGGGTGCTCTAGATGAAAAGCGGGGAGGCCGTCAGGCCTCCCCGCTTTGTTTTTCAGCGCTATCAAAGGTAATTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGTTAAGTAGTTAAGGCA  >  NC_002947/170692‑170988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑