BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,194,928	A→T	5.5%	Q354L (CAG→CTG)	uxpA →	lipoprotein UxpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,194,928	0	A	T	5.5%	747.9 / 22.9	217	Q354L (CAG→CTG)	uxpA	lipoprotein UxpA
Reads supporting (aligned to +/- strand): ref base A (115/90); new base T (6/6); total (121/96)
Fisher's exact test for biased strand distribution p-value = 7.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.56e-01

Only 100 of 222 total aligned reads displayed.

CGGCTGCCGGGGCAGCGGTGCCTTCCGCCTGCGCTGCCAGCGTGTGGCCGGCCAGTGGCAGTTCGACGGGCGTTTTTTCCCGGCCTTTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGG  >  NC_002947/1194779‑1195077

cGGCTGCCGGGGCAGCGGTGCCTTCCGCCTGCGCTGCCAGCGTGTGGCCGGCCAGTGGCAGTTCGACGGGCGTTTTTTCCCGGCCTTTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCa                                                                                                                                                       <  1:394886/150‑1 (MQ=255)

  gCTGCCGGGGCAGCGGTGCCTTCCGCCTGCGCTGCCAGCGTGTGGCCGGCCAGTGGCAGTTCGACGGGCGTTTTTTCCCGGCCTTTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGc                                                                                                                                                     >  1:335102/1‑150 (MQ=255)

      ccGGGGCAGCGGTGCCTTCCGCCTGCGCTGCCAGCGTGTGGCCGGCCAGTGGCAGTTCGACGGGCGTTTTTTCCCGGCCTTTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACg                                                                                                                                                 <  1:3236856/150‑1 (MQ=255)

        ggggCAGCGGTGCCTTCCGCCTGCGCTGCCAGCGTGTGGCCGGCCAGTGGCAGTTCGACGGGCGTTTTTTCCCGGCCTTTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAggccccag                                                                                                                                               >  2:756771/1‑143 (MQ=255)

        ggggCAGCGGTGCCTTCCGCCTGCGCTGCCAGCGTGTGGCCGGCCAGTGGCAGTTCGACGGGCGTTTTTTCCCGGCCTTTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACggg                                                                                                                                               <  2:105479/150‑1 (MQ=255)

         gggCAGCGGGGCCTTCCGCCTGCGCTGCCAGCGTGGGGCCGGCCAGTGGCAGGTCGACGGGCGTTTTGTCCCGGCCATTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAGTTGCTGGCGCAGTTGCGCCTGCaattgcg                                                                                                                                              <  1:4437634/150‑6 (MQ=255)

          ggCAGCGGTGCCTTCCGCCTGCGCTGCCAGCGTGTGGCCGGCCAGTGGCAGTTCGACGGGCGTTTTTTCCCGGCCTTTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCTGGCGCAGTTGCGCCTGCaattgcgc                                                                                                                                             <  2:1826279/150‑7 (MQ=255)

           gCAGCGGTGCCTTCCGCCTGCGCTGCCAGCGTGTGGCCGGCCAGTGGCAGTTCGACGGGCGTTTTTTCCCGGCCTTTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGggccg                                                                                                                                            <  2:3949297/150‑1 (MQ=255)

            cAGCGGTGCCTTCCGCCTGCGCTGCCAGCGTGTGGCCGGCCAGTGGCAGTTCGACGGGCGTTTTTTCCCGGCCTTTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGggccgg                                                                                                                                           <  1:1429506/150‑1 (MQ=255)

             aGCGGTGCCTTCCGCCTGCGCTGCCAGCGTGTGGCCGGCCAGTGGCAGTTCGACGGGCGTTTTTTCCCGGCCTTTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGggccggc                                                                                                                                          <  1:4291712/150‑1 (MQ=255)

             aGCGGTGCCCTCCGCCTGCGCTGCCAGCGTGTGGCCGGCCAGTGGCAGTTCGACGGGCGTTTTTTCCCGGCCTTTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGggccggc                                                                                                                                          >  1:1352255/1‑150 (MQ=255)

               cGGTGCCTTCCGCCTGCGCTGCCAGCGTGTGGCCGGCCAGTGGCAGTTCGACGGGCGTTTTTTCCCGGCCTTTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCa                                                                                                                                        >  1:3205582/1‑150 (MQ=255)

               cGGTGCCTTCCGCCTGCGCTGCCAGCGTGTGGCCGGCCAGTGGCAGTTCGACGGGCGTTTTTTCCCGGCCTTTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCa                                                                                                                                        >  1:1182030/1‑150 (MQ=255)

                    ccTTCCGCCTGCGCTGCCAGCGTGTGGCCGGCCAGTGGCAGTTCGACGGGCGTTTTTTCCCGGCCTTTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCgg                                                                                                                                   <  1:4166120/150‑1 (MQ=255)

                     cTTCCGCCTGCGCTGCCAGCGTGTGGCCGGCCAGTGGCAGTTCGACGGGCGTTTTTTCCCGGCCTTTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGc                                                                                                                                  >  1:3274546/1‑150 (MQ=255)

                      ttCCGCCTGCGCTGCCAGCGTGTGGCCGGCCAGTGGCAGTTCGACGGGCGTTTTTTCCCGGCCTTTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGcc                                                                                                                                 >  2:4362266/1‑150 (MQ=255)

                      ttCCGCCTGCGCTGCCAGCGTGTGGCCGGCCAGTGGCAGTTCGACGGGCGTTTTTTCCCGGCCTTTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGcc                                                                                                                                 >  1:1108088/1‑150 (MQ=255)

                       tCCGCCTGCGCTGCCAGCGTGTGGCCGGCCAGTGGCAGTTCGACGGGCGTTTTTTCCCGGCCTTTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCt                                                                                                                                <  1:1145359/150‑1 (MQ=255)

                        ccGCCTGCGCTGCCAGCGTGTGGCCGGCCAGTGGCAGTTCGACGGGCGTTTTTTCCCGGCCTTTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTg                                                                                                                               <  1:1688556/150‑1 (MQ=255)

                            cTGCGCTGCCAGCGTGTGGCCGGCCAGTGGCAGTTCGACGGGCGTTTTTTCCCGGCCTTTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTc                                                                                                                           <  2:4000852/150‑1 (MQ=255)

                               cgcTGCCAGCGTGTGGCCGGCCAGTGGCAGTTCGACGGGCGTTTTTTCCCGGCCTTTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGAc                                                                                                                        >  2:2019910/1‑150 (MQ=255)

                                gcTGCCAGCGTGTGGCCGGCCAGTGGCAGTTCGACGGGCGTTTTTTCCCGGCCTTTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGAcc                                                                                                                       >  1:1321399/1‑150 (MQ=255)

                                        cGTGTGGCCGGCCAGTGGCAGTTCGACGGGCGTTTTTTCCCGGCCTTTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTg                                                                                                               <  2:1218974/150‑1 (MQ=255)

                                             ggccggccAGTGGCAGTTCGACGGGCGTTTTTTCCCGGCCTTTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCcg                                                                                                          >  1:2710489/1‑150 (MQ=255)

                                             ggccggccAGTGGCAGTTCGACGGGCGTTTTTTCCCGGCCTTTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCcg                                                                                                          >  2:848053/1‑150 (MQ=255)

                                              gccggccAGTGGCAGTTCGACGGGCGTTTTTTCCCGGCCTTTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCcgc                                                                                                         >  1:3581924/1‑150 (MQ=255)

                                              gccggccAGTGGCAGTTCGACGGGCGTTTTTTCCCGGCCTTTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCcgc                                                                                                         >  1:4496387/1‑150 (MQ=255)

                                              gccggccAGTGGCAGTTCGACGGGCGTTTTTTCCCGGCCTTTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCGGGCTCGACCAGCCGCTGGCCcgc                                                                                                         >  1:2108995/1‑150 (MQ=255)

                                               ccggccAGTGGCAGTTCGACGGGCGTTTTTTCCCGGCCTTTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGggccg                                                                                                                                            <  1:1753961/114‑1 (MQ=255)

                                                cggccAGTGGCAGTTCGACGGGCGTTTTTTCCCGGCCTTTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCcgcgc                                                                                                       >  2:146899/1‑150 (MQ=255)

                                                 ggccAGTGGCAGTTCGACGGGCGTTTTTTCCCGGCCTTTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCCGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCcgcgcg                                                                                                      >  1:794847/1‑150 (MQ=255)

                                                 ggccAGTGGCAGTTCGACGGGCGTTTTTTCCCGGCCTTTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCcgcgcg                                                                                                      >  2:4471336/1‑150 (MQ=255)

                                                 ggccAGTGGCAGTTCGACGGGCGTTTTTTCCCGGCCTTTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCcgcgcg                                                                                                      >  2:3020891/1‑150 (MQ=255)

                                                       tGGCAGTTCGACGGGCGTTTTTTCCCGGCCTTTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAg                                                                                                <  1:178509/150‑1 (MQ=255)

                                                        ggCAGTTCGACGGGCGTTTTTTCCCGGCCTTTGAACAGCAACTGGCTCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCTCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCTCGCGCCCCAgg                                                                                               <  1:249915/150‑1 (MQ=255)

                                                         gCAGTTCGACGGGCGTTTTTTCCCGGCCTTTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGc                                                                                              >  1:3508311/1‑150 (MQ=255)

                                                             ttCGACGGGCGTTTTTTCCCGGCCTTTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCTGGCGCAGTTGCGCCTGc                                                                                                                                                     >  2:2361024/1‑91 (MQ=255)

                                                             ttCGACGGGCGTTTTTTCCCGGCCTTTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCTGGCGCAGTTGCGCCTGc                                                                                                                                                     <  1:2361024/91‑1 (MQ=255)

                                                             ttCGACGGGCGTTTTTTCCCGGCCTTTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCGtg                                                                                          >  2:269261/1‑150 (MQ=255)

                                                              tCGACGGGCGTTTTTTCCCGGCCTTTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTtgt                                                                                         <  1:430614/150‑1 (MQ=255)

                                                               cGACGGGCGTGTTTTCCCGGCCTTTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTtgtg                                                                                        <  1:850373/150‑1 (MQ=255)

                                                                    ggCGTTTTTTCCCGGCCTTTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGcgcc                                                                                   >  2:1588762/1‑150 (MQ=255)

                                                                     gCGTTTTTTCCCGGCCTTTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGcgccg                                                                                  >  2:2772400/1‑150 (MQ=255)

                                                                      cGTTTTTTCCCGGCCATTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGcgccgc                                                                                 <  2:4221568/150‑1 (MQ=255)

                                                                       gTTTTTTCCCGGCCTTTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCcgcg                                                                                >  1:3010120/1‑150 (MQ=255)

                                                                        ttttttCCCGGCCTTTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGa                                                                               >  1:2772757/1‑150 (MQ=255)

                                                                        ttttttCCCGGCCTTTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGa                                                                               >  2:4420243/1‑150 (MQ=255)

                                                                         tttttCCCGGCCTTTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGAt                                                                              <  2:2118736/150‑1 (MQ=255)

                                                                            ttCCCGGCCTTTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCATGTGGCGCCGCGATccc                                                                           >  2:3847966/1‑150 (MQ=255)

                                                                               ccGGCCTTTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGt                                                                        >  2:2713920/1‑150 (MQ=255)

                                                                               ccGGCCTTTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGt                                                                        >  1:3032407/1‑150 (MQ=255)

                                                                                cGGCCTTTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTg                                                                       >  1:3989927/1‑150 (MQ=255)

                                                                                cGGCCTTTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTg                                                                       >  1:3847106/1‑150 (MQ=255)

                                                                                     tttGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCCCCGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCGGGTGGCGCCGCGATACCCGTGGCGGt                                                                  >  2:1704080/1‑150 (MQ=255)

                                                                                          aCAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTg                                                             <  2:2943192/150‑1 (MQ=255)

                                                                                          aCAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTg                                                             <  1:534062/150‑1 (MQ=255)

                                                                                          aCAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTg                                                             >  2:796808/1‑150 (MQ=255)

                                                                                           cAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTgg                                                            <  1:694629/150‑1 (MQ=255)

                                                                                            aGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTggg                                                           >  1:3553237/1‑150 (MQ=255)

                                                                                            aGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTggg                                                           >  1:720154/1‑150 (MQ=255)

                                                                                            aGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTggg                                                           >  1:719981/1‑150 (MQ=255)

                                                                                             gCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCt                                                                                                                <  2:3582936/96‑1 (MQ=255)

                                                                                             gCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGa                                                          <  1:958520/150‑1 (MQ=255)

                                                                                             gCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGa                                                          <  2:3391492/150‑1 (MQ=255)

                                                                                             gCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGa                                                          <  1:1026474/150‑1 (MQ=255)

                                                                                               aaCTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGCTACCCGTGGCGGTAGCTGGGAct                                                        <  2:377323/150‑1 (MQ=255)

                                                                                               aaCTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGAct                                                        <  1:3744724/150‑1 (MQ=255)

                                                                                                 cTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGActgc                                                      <  2:3274546/150‑1 (MQ=255)

                                                                                                 cTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGActgc                                                      <  1:658066/150‑1 (MQ=255)

                                                                                                  tGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGActgcc                                                     >  1:622894/1‑150 (MQ=255)

                                                                                                     cgcCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGActgcctgc                                                  <  1:2611374/150‑1 (MQ=255)

                                                                                                      gcCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCctgct                                                 <  2:919482/150‑1 (MQ=255)

                                                                                                      gcCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCctgct                                                 <  1:154300/150‑1 (MQ=255)

                                                                                                             gcgcAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCaa                                          >  2:3228002/1‑150 (MQ=255)

                                                                                                             gcgcAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCaa                                          <  2:1198790/150‑1 (MQ=255)

                                                                                                                 aGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCt                                      <  1:490951/150‑1 (MQ=255)

                                                                                                                  gCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCtt                                     <  2:3265785/150‑1 (MQ=255)

                                                                                                                   cTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTg                                    >  1:4348522/1‑150 (MQ=255)

                                                                                                                   cTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTg                                    >  2:1512498/1‑150 (MQ=255)

                                                                                                                    tGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTgg                                   <  2:243820/150‑1 (MQ=255)

                                                                                                                    tGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTgg                                   <  2:3205582/150‑1 (MQ=255)

                                                                                                                        tgtCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCcga                               <  2:1698122/150‑1 (MQ=255)

                                                                                                                           cggcccgttGCTGGCGCAGTTGCGCCTGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCGTGTGGCGCCGCGATACCCCTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCATGGCcgacga                            >  2:421391/8‑150 (MQ=255)

                                                                                                                            gcgcAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGAt                           >  2:605188/1‑150 (MQ=255)

                                                                                                                            gcgcAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGAt                           >  2:2868832/1‑150 (MQ=255)

                                                                                                                                aaTTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATagca                       >  2:2219531/1‑150 (MQ=255)

                                                                                                                                aaTTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATagca                       >  2:4483900/1‑150 (MQ=255)

                                                                                                                                 gttGCTGGCGCAGTTGCGCCTGCAACGGGCCGGCCACTCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATagcag                      >  1:1292750/2‑150 (MQ=255)

                                                                                                                                 gttGCTGGCGCAGTTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCGGGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATagcag                      >  2:2402051/2‑150 (MQ=255)

                                                                                                                                  ttGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATagcagc                     <  1:3969478/150‑1 (MQ=255)

                                                                                                                                    gctggCGCAGTTGCGCCTGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCAt                   >  1:3198773/4‑150 (MQ=255)

                                                                                                                                    gCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCAt                   >  1:2903016/1‑150 (MQ=255)

                                                                                                                                     cAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATg                  >  2:1115711/1‑150 (MQ=255)

                                                                                                                                      aGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGc                 >  1:360513/1‑150 (MQ=255)

                                                                                                                                        gcgcAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGccc               >  2:3360031/1‑150 (MQ=255)

                                                                                                                                          gcAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCgt             <  1:1150374/150‑1 (MQ=255)

                                                                                                                                            aaCTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCgtgt           <  2:1324735/150‑1 (MQ=255)

                                                                                                                                                    cAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCg   >  1:2653345/1‑150 (MQ=255)

                                                                                                                                                    cAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCg   >  2:2210923/1‑150 (MQ=255)

                                                                                                                                                     aGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCgg  <  1:769065/150‑1 (MQ=255)

CGGCTGCCGGGGCAGCGGTGCCTTCCGCCTGCGCTGCCAGCGTGTGGCCGGCCAGTGGCAGTTCGACGGGCGTTTTTTCCCGGCCTTTGAACAGCAACTGGCGCCTGCCGCGCAGCTGCGTGTCGCGCAATTGCAGGCGCAACTGCGCCAGCAACGGGCCGGCCACGCGGCCTGGCTCGACCAGCCGCTGGCCCGCGCGCCCCAGGCCTTGTGGCGCCGCGATACCCGTGGCGGTAGCTGGGACTGCCTGCTGCATCAAGCCTTGGCCGACGATAGCAGCATGCCCGTGTTGCTGCCGG  >  NC_002947/1194779‑1195077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`