BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,285,034	G→A	5.0%	G87S (GGT→AGT) ‡	PP_1122 →	OmpA family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,285,034	0	G	A	5.0%	655.7 / 15.4	199	G87S (GGT→AGT) ‡	PP_1122	OmpA family protein
Reads supporting (aligned to +/- strand): ref base G (99/90); new base A (7/3); total (106/93)
Fisher's exact test for biased strand distribution p-value = 3.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 220 total aligned reads displayed.

TGGTGGCTGCCAGCCTGCTCACGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACTGAAACA  >  NC_002947/1284885‑1285182

tggtggCTGCCAGCCTGCTCACGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCggtg                                                                                                                                                      <  2:194408/150‑1 (MQ=255)

tggtggCTGCCAGCCTGCTCACGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCggtg                                                                                                                                                      <  1:2945501/150‑1 (MQ=255)

  gtggCTGCCAGCCTGCTCACGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCggtggt                                                                                                                                                    >  1:4035623/1‑150 (MQ=255)

  gtggCTGCCAGCCTGCTCACGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCggtggt                                                                                                                                                    >  1:2827497/1‑150 (MQ=255)

   tggCTGCCAGCCTGCTCACGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGgtggtg                                                                                                                                                   <  1:3385688/150‑1 (MQ=255)

          cAGCCTGCTCACGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCtt                                                                                                                                            <  1:2112633/150‑1 (MQ=255)

            gCCTGCTCACGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTgg                                                                                                                                          <  1:2655214/150‑1 (MQ=255)

            gCCTGCTCACGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTgg                                                                                                                                          <  1:1190845/150‑1 (MQ=255)

            gCCTGCTCACGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCGGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTgg                                                                                                                                          <  1:4261597/150‑1 (MQ=255)

              cTGCTCACGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGgc                                                                                                                                        <  1:1447072/150‑1 (MQ=255)

                gCTCACGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGgcgc                                                                                                                                      <  1:3864720/150‑1 (MQ=255)

                  tCACGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGccc                                                                                                                                    >  1:3474651/1‑149 (MQ=255)

                    aCGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCAtt                                                                                                                                  <  2:1037886/150‑1 (MQ=255)

                    aCGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCAtt                                                                                                                                  <  1:4252060/150‑1 (MQ=255)

                        gCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCcg                                                                                                                              <  1:3677457/150‑1 (MQ=255)

                         cTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGCAATGGCGCCCAAGGCGCCaccaccgg                                                                                                                             <  1:4129694/150‑8 (MQ=255)

                            cgcTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCcggcgg                                                                                                                          >  1:505745/1‑150 (MQ=255)

                               tACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCt                                                                                                                       >  2:3987292/1‑150 (MQ=255)

                               tACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCt                                                                                                                       <  2:2666544/150‑1 (MQ=255)

                               tACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCGTGGGCGCCCTTGCCGGCGGGcg                                                                                                                       >  1:427950/1‑149 (MQ=255)

                               gtagcgcaGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCt                                                                                                                       >  1:3902032/7‑150 (MQ=255)

                                aCGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGCCCGCCGGCAATGGCGCCCAAGGCGCCACCACcggcggccca                                                                                                                      <  2:795950/150‑5 (MQ=37)

                                 cGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGa                                                                                                                     >  1:2390446/1‑150 (MQ=255)

                                  gCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGAt                                                                                                                    >  1:946475/1‑150 (MQ=255)

                                  gCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTGGGGCGCCCTTGCCGGCGGGCGGAt                                                                                                                    >  1:2008154/1‑150 (MQ=255)

                                   cacaGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGAtt                                                                                                                   <  2:2377340/150‑1 (MQ=255)

                                    acaGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGAttt                                                                                                                  >  1:458756/1‑150 (MQ=255)

                                    acaGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGAttt                                                                                                                  >  1:374600/1‑150 (MQ=255)

                                    acaGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGAttt                                                                                                                  >  2:3658946/1‑150 (MQ=255)

                                    acaGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCAACAGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCCTTGACGGCGGGCTGAttt                                                                                                                  >  1:4231791/1‑150 (MQ=255)

                                            ggCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGcc                                                                                                          <  1:1880521/150‑1 (MQ=255)

                                            ggCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCATGGGCGCCATTGCCGGCGGGCTGATTTGTTACGcc                                                                                                          >  1:2860462/1‑150 (MQ=255)

                                             gCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGccc                                                                                                         <  1:2401885/150‑1 (MQ=255)

                                             gCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGccc                                                                                                         <  2:1678528/150‑1 (MQ=255)

                                              cAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCa                                                                                                        <  2:2259344/150‑1 (MQ=255)

                                              cAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCa                                                                                                        <  1:987321/150‑1 (MQ=255)

                                              cAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCa                                                                                                        <  1:2246506/150‑1 (MQ=255)

                                               aGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAg                                                                                                       <  2:3248098/150‑1 (MQ=255)

                                               aGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAg                                                                                                       <  2:2823923/150‑1 (MQ=255)

                                                 tgcgcCCCACAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCGTGGGCGCCATTGCcggcg                                                                                                                           >  1:3668786/2‑128 (MQ=255)

                                                      cccTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTg                                                                                                <  1:2121825/150‑1 (MQ=255)

                                                       ccTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGa                                                                                               <  1:2169912/150‑1 (MQ=255)

                                                       ccTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGa                                                                                               <  2:12096/150‑1 (MQ=255)

                                                        cTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGAc                                                                                              >  2:3500399/1‑150 (MQ=255)

                                                            atcaaAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAg                                                                                          >  1:2598899/1‑150 (MQ=255)

                                                             tcaaAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGa                                                                                         <  2:3780007/150‑1 (MQ=255)

                                                               aaaGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGGTGTTTTGTTAAGCCCAGGACGGTGACGAAGATg                                                                                       >  1:692994/1‑150 (MQ=255)

                                                               aaaGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATg                                                                                       >  2:2758997/1‑150 (MQ=255)

                                                                aaGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATgg                                                                                      >  2:4033502/1‑150 (MQ=255)

                                                                     ccTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACg                                                                                 <  2:2134694/150‑1 (MQ=255)

                                                                     ccTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACg                                                                                 <  1:829644/150‑1 (MQ=255)

                                                                      cTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACgg                                                                                >  1:4403275/1‑150 (MQ=255)

                                                                      cTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACgg                                                                                >  2:2741849/1‑150 (MQ=255)

                                                                         gCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCAt                                                                             >  1:2796966/1‑150 (MQ=255)

                                                                           ccATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCAATGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCAttt                                                                           >  1:575579/1‑150 (MQ=255)

                                                                            cATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCAtttt                                                                          <  1:161070/150‑1 (MQ=255)

                                                                            cATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCAtttt                                                                          <  1:3781667/150‑1 (MQ=255)

                                                                                tGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGAc                                                                      >  1:1790661/1‑150 (MQ=255)

                                                                                   aGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCgg                                                                   <  2:3438806/150‑1 (MQ=255)

                                                                                     ccTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGgcg                                                                 >  1:1425495/1‑150 (MQ=255)

                                                                                      ctgctgGGCGGCTTGGTCGGTGGTGACCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGgcgc                                                                <  1:311549/150‑1 (MQ=255)

                                                                                           gggCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCa                                                           <  2:4215877/150‑1 (MQ=255)

                                                                                              cggcTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTg                                                        >  1:2557896/1‑150 (MQ=255)

                                                                                               ggcTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGc                                                       <  2:3767416/150‑1 (MQ=255)

                                                                                                      tCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCgaga                                                >  1:3594388/1‑150 (MQ=255)

                                                                                                      tCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCgaga                                                >  1:2953155/1‑150 (MQ=255)

                                                                                                       cGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCC‑TGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGAcc                                              <  1:2770363/150‑1 (MQ=255)

                                                                                                           ggtggCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCg                                           <  1:1329872/150‑1 (MQ=255)

                                                                                                           ggtggCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCg                                           <  1:2284914/150‑1 (MQ=255)

                                                                                                                 cTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAac                                     >  2:1888629/1‑150 (MQ=255)

                                                                                                                    ggTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACAcg                                  <  1:4481053/150‑1 (MQ=255)

                                                                                                                      tGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACAcggc                                >  2:4522772/1‑150 (MQ=255)

                                                                                                                         cATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCGGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTAGACCGGCGCGATCACTGCCCCCAGACCCCGGCCAACACGGCGGt                             >  1:2287484/1‑150 (MQ=255)

                                                                                                                          aTCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTTCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGtt                            >  2:406574/1‑150 (MQ=255)

                                                                                                                          aTCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGtt                            >  2:2051871/1‑150 (MQ=255)

                                                                                                                          aTCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGtt                            <  2:2658114/150‑1 (MQ=255)

                                                                                                                            cGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGa                          <  2:1549567/150‑1 (MQ=255)

                                                                                                                            cGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGa                          <  1:856316/150‑1 (MQ=255)

                                                                                                                             gagaGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGAc                         >  2:1098174/1‑150 (MQ=255)

                                                                                                                             gagaGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGAc                         >  2:3008653/1‑150 (MQ=255)

                                                                                                                              agagTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGAcc                        <  2:4144350/150‑1 (MQ=255)

                                                                                                                               gagTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACca                       <  1:4088040/150‑1 (MQ=255)

                                                                                                                                  ttcttcCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACAt                    >  2:2474265/1‑150 (MQ=255)

                                                                                                                                  ttcttcCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACAt                    <  1:1475219/150‑1 (MQ=255)

                                                                                                                                    cttcCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATgg                  <  1:3478307/150‑1 (MQ=255)

                                                                                                                                     ttcCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATggg                 >  2:3867573/1‑150 (MQ=255)

                                                                                                                                       ccTGGGCCGCCGGTGGTGGCGCCGTGGGCGCCCATGCCGGCGGGCTGATTTGTTACGCCCCGGACGGGGACGAAGATGGTGACGGCATTTTCGACCCGCGCGATCACAGCCCCCAGACCCCCGCCAACACCGCGGTTGACCCCCAGGGCt               >  1:598338/1‑150 (MQ=255)

                                                                                                                                        cTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTg              <  2:598338/150‑1 (MQ=255)

                                                                                                                                          gggCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGcc            <  2:2805459/150‑1 (MQ=255)

                                                                                                                                          agcccgccgGCAATGGCGCCCAAGGCGCCACCACCGGCGGCCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGcc            >  2:470506/4‑150 (MQ=37)

                                                                                                                                           ggCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGccc           >  2:4305677/1‑150 (MQ=255)

                                                                                                                                            gccgccGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCa          <  1:1169900/150‑1 (MQ=255)

                                                                                                                                            gccgccGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATGTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCGGCCCa          >  1:1102868/1‑150 (MQ=255)

                                                                                                                                             ccgccgGCAATGGCGCCCAAGGCGCCACCACCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCAc         >  2:2595451/1‑150 (MQ=38)

                                                                                                                                             ccgccgGCAATGGCGCCCAAGGCGCCACCACCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCAc         >  2:711001/1‑150 (MQ=38)

                                                                                                                                                ccgGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACTGa      <  2:3109688/150‑1 (MQ=255)

                                                                                                                                                  ggtggtGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACTGaaa    <  1:1067051/150‑1 (MQ=255)

                                                                                                                                                    tggtggCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACTGAAACa  <  1:4119414/150‑1 (MQ=255)

                                                                                                                                                    tggtggCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACTGAAACa  <  1:3983529/150‑1 (MQ=255)

                                                                                                                                                    tggtggCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACTGAAACa  <  2:1295823/150‑1 (MQ=255)

TGGTGGCTGCCAGCCTGCTCACGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACTGAAACA  >  NC_002947/1284885‑1285182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`