Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,285,043 T→C 6.0% L90L (TTG→CTG) ‡ PP_1122 → OmpA family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,285,0430TC 6.0% 669.5 / 21.3 220L90L (TTG→CTG) ‡PP_1122OmpA family protein
Reads supporting (aligned to +/- strand):  ref base T (97/102);  new base C (9/4);  total (113/107)
Fisher's exact test for biased strand distribution p-value = 2.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.17e-01

Only 100 of 221 total aligned reads displayed.

CAGCCTGCTCACGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACTGAAACAGTACCCG  >  NC_002947/1284895‑1285189
                                                                                                                                                    |                                                                                                                                                  
 aGCCTGCTCACGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGAGGTGGCGCCTTg                                                                                                                                                  >  2:280698/1‑150 (MQ=255)
  gCCTGCTCACGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTgg                                                                                                                                                 <  1:1190845/150‑1 (MQ=255)
      gCTCACGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGgcgc                                                                                                                                             <  1:3864720/150‑1 (MQ=255)
      gCTCACGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGgcgc                                                                                                                                             >  1:2622835/1‑150 (MQ=255)
        tCACGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCa                                                                                                                                           >  2:2081726/1‑150 (MQ=255)
          aCGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCAtt                                                                                                                                         <  1:4252099/150‑1 (MQ=255)
          aCGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCAtt                                                                                                                                         <  2:1037935/150‑1 (MQ=255)
            gggCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGc                                                                                                                                       >  1:4420162/1‑150 (MQ=255)
            gggCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGCAATGGCGCCCAAGGCGCCaccac                                                                                                                                       <  1:1709889/150‑5 (MQ=255)
              gCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCcg                                                                                                                                     <  1:3677457/150‑1 (MQ=255)
               cTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCcgg                                                                                                                                    >  1:2805459/1‑150 (MQ=255)
               cTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCcgg                                                                                                                                    <  2:2202510/150‑1 (MQ=255)
               cTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGCAATGGCGCCCAAGGCGCCaccaccgg                                                                                                                                    <  1:4129694/150‑8 (MQ=255)
                  cgcTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCcggcgg                                                                                                                                 <  1:4075294/150‑1 (MQ=255)
                    cTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGc                                                                                                                               >  2:710749/1‑150 (MQ=255)
                     tACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCt                                                                                                                              <  2:2666544/150‑1 (MQ=255)
                      aCGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGCCCGCCGGCAATGGCGCCCAAGGCGCCACCACcggcggccca                                                                                                                             <  2:795950/150‑5 (MQ=37)
                       cGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGa                                                                                                                            >  1:2390446/1‑150 (MQ=255)
                        gCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGAt                                                                                                                           >  2:598246/1‑150 (MQ=255)
                        gCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTGGGGCGCCCTTGCCGGCGGGCGGAt                                                                                                                           >  1:2008154/1‑150 (MQ=255)
                         cacaGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGAtt                                                                                                                          <  2:2377340/150‑1 (MQ=255)
                          acaGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGAttt                                                                                                                         >  1:4244236/1‑150 (MQ=255)
                          acaGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGAttt                                                                                                                         >  2:3658946/1‑150 (MQ=255)
                          acaGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGAttt                                                                                                                         <  1:1859138/150‑1 (MQ=255)
                          acaGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGAttt                                                                                                                         >  1:374600/1‑150 (MQ=255)
                           caGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTg                                                                                                                        >  2:3781667/1‑150 (MQ=255)
                              cGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTa                                                                                                                     >  2:2000364/1‑150 (MQ=255)
                               gATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCGGATTTGTTAc                                                                                                                    >  1:449579/1‑150 (MQ=255)
                                aTGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACg                                                                                                                   >  2:3445970/1‑150 (MQ=255)
                                  ggCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCATGGGCGCCATTGCCGGCGGGCTGATTTGTTACGcc                                                                                                                 >  1:2860462/1‑150 (MQ=255)
                                   gCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGccc                                                                                                                <  2:1678528/150‑1 (MQ=255)
                                    cAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCa                                                                                                               <  1:987321/150‑1 (MQ=255)
                                    cAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCa                                                                                                               <  1:2246506/150‑1 (MQ=255)
                                             ccTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGa                                                                                                      <  1:1974230/150‑1 (MQ=255)
                                             ccTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGa                                                                                                      <  2:12096/150‑1 (MQ=255)
                                             ccTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCGGATTTGTTACGCCCAGGACGGTGa                                                                                                      >  2:1030908/1‑150 (MQ=255)
                                              cTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTgtc                                                                                                     >  1:125252/1‑148 (MQ=255)
                                               tcaatcaaAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACg                                                                                                    >  2:3523894/1‑150 (MQ=255)
                                               tcaatcaaAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCATGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACg                                                                                                    >  1:1541287/1‑150 (MQ=255)
                                                  atcaaAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAg                                                                                                 >  2:3990925/1‑150 (MQ=255)
                                                   tcaaAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCCTTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGa                                                                                                >  1:396726/1‑150 (MQ=255)
                                                     aaaGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATg                                                                                              >  2:2758997/1‑150 (MQ=255)
                                                     aaaGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATg                                                                                              >  2:3135678/1‑150 (MQ=255)
                                                      aaGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATgg                                                                                             >  2:1475219/1‑150 (MQ=255)
                                                      aaGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCAGTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATgg                                                                                             >  1:3106590/1‑150 (MQ=255)
                                                            cTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACgg                                                                                       >  1:4403275/1‑150 (MQ=255)
                                                            cTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACgg                                                                                       >  2:2741849/1‑150 (MQ=255)
                                                               gCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCAt                                                                                    >  1:2796966/1‑150 (MQ=255)
                                                                 ccATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCAttt                                                                                  >  1:2118571/1‑150 (MQ=255)
                                                                  cATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCAtttt                                                                                 <  1:3781667/150‑1 (MQ=255)
                                                                  cATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCAtttt                                                                                 >  1:1367283/1‑150 (MQ=255)
                                                                  cATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCAtttt                                                                                 >  1:2163698/1‑150 (MQ=255)
                                                                        cAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCg                                                                           >  2:2635008/1‑150 (MQ=255)
                                                                              gctgGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGa                                                                     <  1:3672254/150‑1 (MQ=255)
                                                                              gctgGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGa                                                                     <  1:272752/150‑1 (MQ=255)
                                                                                 gggCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCa                                                                  <  1:511827/150‑1 (MQ=255)
                                                                                 gggCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCa                                                                  <  2:4215877/150‑1 (MQ=255)
                                                                                    cggcTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTg                                                               >  1:2557896/1‑150 (MQ=255)
                                                                                     ggcTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGc                                                              <  2:3767462/150‑1 (MQ=255)
                                                                                        ttGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTTGGCGCCAATGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCAATTTAGACCGGCGCGATAACTGcccc                                                           >  1:800973/1‑150 (MQ=255)
                                                                                        ttGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGcccc                                                           >  2:3346668/1‑150 (MQ=255)
                                                                                            tCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCCTTTTCGACCGGCGCGATCACTGCCCCgaga                                                       >  2:1587936/1‑150 (MQ=255)
                                                                                            tCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCgaga                                                       >  1:3594388/1‑150 (MQ=255)
                                                                                                 ggtggCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCg                                                  <  1:2284914/150‑1 (MQ=255)
                                                                                                      ccTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCaa                                             <  1:1474105/150‑1 (MQ=255)
                                                                                                       cTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAac                                            >  2:1888629/1‑150 (MQ=255)
                                                                                                        ttGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAaca                                           <  1:3467484/150‑1 (MQ=255)
                                                                                                          ggTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCCGCCAACAcg                                         >  1:2435249/1‑150 (MQ=255)
                                                                                                           gTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACAcgg                                        >  1:671207/1‑150 (MQ=255)
                                                                                                             gCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACAcggcg                                      <  1:2947907/150‑1 (MQ=255)
                                                                                                              ccATCGAGAGTTCGTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAATAcggcgg                                     <  2:4020290/150‑1 (MQ=255)
                                                                                                               cATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCGGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTAGACCGGCGCGATCACTGCCCCCAGACCCCGGCCAACACGGCGGt                                    >  1:2287484/1‑150 (MQ=255)
                                                                                                                aTCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTTCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGtt                                   >  2:406574/1‑150 (MQ=255)
                                                                                                                  cGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGa                                 <  1:856223/150‑1 (MQ=255)
                                                                                                                   gagaGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGAc                                >  2:3008653/1‑150 (MQ=255)
                                                                                                                    agagTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGAcc                               <  2:4144350/150‑1 (MQ=255)
                                                                                                                       gTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCGGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACcaca                            >  2:224741/1‑150 (MQ=255)
                                                                                                                        ttcttcCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGCCGGCATTTTCGACCGGCGCGATAACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACAt                           >  2:3964058/1‑150 (MQ=255)
                                                                                                                        ttcttcCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACAt                           >  2:2474265/1‑150 (MQ=255)
                                                                                                                         tcttcCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATg                          <  2:440432/150‑1 (MQ=255)
                                                                                                                          cttcCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATgg                         <  1:3478225/150‑1 (MQ=255)
                                                                                                                            tcCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGc                       <  2:2661693/150‑1 (MQ=255)
                                                                                                                             ccTGGGCCGCCGGTGGTGGCGCCGTGGGCGCCCATGCCGGCGGGCTGATTTGTTACGCCCCGGACGGGGACGAAGATGGTGACGGCATTTTCGACCCGCGCGATCACAGCCCCCAGACCCCCGCCAACACCGCGGTTGACCCCCAGGGCt                      >  1:598338/1‑150 (MQ=255)
                                                                                                                               tGGGCCGCCGGTGGTGGCGCCGTGGGCGCCAATTCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCCGCCAACACGGCGGTTGACCACAAGGGCTGc                    >  1:4262196/1‑150 (MQ=255)
                                                                                                                                agcccgccgGCAATGGCGCCCAAGGCGCCACCACCGGCGGCCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGcc                   >  2:470275/4‑150 (MQ=37)
                                                                                                                                 ggCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGccc                  >  2:4305677/1‑150 (MQ=255)
                                                                                                                                  gccgccGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCa                 <  1:1169900/150‑1 (MQ=255)
                                                                                                                                  gccgccGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCa                 <  1:877381/150‑1 (MQ=255)
                                                                                                                                  gccgccGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATGTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCGGCCCa                 >  1:1102868/1‑150 (MQ=255)
                                                                                                                                    cgccgGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACt               <  1:3280924/150‑1 (MQ=255)
                                                                                                                                     gccgGTGGTGGCGCCTTGGGCGCCATTTCCGGCGGGCTGATTTGTTACGCCCAGGAAGGTGACGAAGATGGTGAAGGCATTTTAGACCGGCGCGATCACTGCCCCCAGACCCCGGCCAACACGGCGGTTGAACACATTGGCTGCCCACTg              >  1:469636/1‑150 (MQ=255)
                                                                                                                                      ccgGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACTGa             <  2:2827497/150‑1 (MQ=255)
                                                                                                                                        ggtggtGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACTGaaa           <  1:1067051/150‑1 (MQ=255)
                                                                                                                                         gtggtgGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACTGAAAc          <  1:1427592/150‑1 (MQ=255)
                                                                                                                                          tggtggCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACTGAAACa         >  1:2214354/1‑150 (MQ=255)
                                                                                                                                          tggtggCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACTGAAACa         <  1:4196219/150‑1 (MQ=255)
                                                                                                                                          tggtggCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACTGAAACa         <  1:4093752/150‑1 (MQ=255)
                                                                                                                                          tggtggCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACTGAAACa         <  1:3983529/150‑1 (MQ=255)
                                                                                                                                             tggCGCCCAAGGCGCCACCACCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACTGAAACAGTa      <  1:358312/150‑1 (MQ=255)
                                                                                                                                                 gcCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACTGAAACAGTACCCg  >  2:3039859/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CAGCCTGCTCACGGGCTGCGCTACGCACAGCGATGGCAGCGCGCCCCTCAATCAAAGGACCTGGCCCATCTGCAGCCTGCTGGGCGGCTTGGTCGGTGGTGGCCTTGGTGCCATCGAGAGTTCTTCCTGGGCCGCCGGTGGTGGCGCCTTGGGCGCCATTGCCGGCGGGCTGATTTGTTACGCCCAGGACGGTGACGAAGATGGTGACGGCATTTTCGACCGGCGCGATCACTGCCCCGAGACCCCGGCCAACACGGCGGTTGACCACATGGGCTGCCCACTGAAACAGTACCCG  >  NC_002947/1284895‑1285189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑