BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,392,933	C→G	16.9%	intergenic (+97/‑89)	PP_1214 → / → ruvC	transcriptional regulator/crossover junction endodeoxyribonuclease RuvC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,392,933	0	C	G	16.9%	362.0 / 63.7	142	intergenic (+97/‑89)	PP_1214/ruvC	transcriptional regulator/crossover junction endodeoxyribonuclease RuvC
Reads supporting (aligned to +/- strand): ref base C (53/59); new base G (10/14); total (69/73)
Fisher's exact test for biased strand distribution p-value = 6.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.08e-01

Only 100 of 143 total aligned reads displayed.

GTCTATTCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGTACGCC  >  NC_002947/1392786‑1393082

 tCTATTCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACG‑CC‑CATTTCaaa                                                                                                                                                     <  2:3778592/150‑1 (MQ=255)

  cTATTCCAATGCCCAGCTTGCCGCCGAGAACTAGGAAAACCTCCGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGAGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGTGTAAACCCGCTCCTACAGGTAGTACG‑CC‑CCTTTCAAAt                                                                                                                                                    <  1:1634907/150‑1 (MQ=255)

   tATTCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAAtg                                                                                                                                                   <  2:1439770/150‑1 (MQ=255)

     ttCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAAtgtg                                                                                                                                                 <  1:463274/150‑1 (MQ=255)

     ttCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAAtgtg                                                                                                                                                 <  1:4190189/150‑1 (MQ=255)

     ttCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAAtgtg                                                                                                                                                 <  1:3560335/150‑1 (MQ=255)

     ttCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAAtgtg                                                                                                                                                 <  2:4193509/150‑1 (MQ=255)

     gtCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCTTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAAtgtg                                                                                                                                                 <  1:3418252/149‑1 (MQ=255)

       ccAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATgtggt                                                                                                                                               <  1:1585910/150‑1 (MQ=255)

       ccAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATgtggt                                                                                                                                               <  2:896129/150‑1 (MQ=255)

        cAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATgtggtg                                                                                                                                              <  2:4363537/150‑1 (MQ=255)

        cAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATgtggtg                                                                                                                                              <  2:1885807/150‑1 (MQ=255)

        cAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATgtggtg                                                                                                                                              <  2:124268/150‑1 (MQ=255)

        cAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCGAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATgtggtg                                                                                                                                              <  2:124308/150‑1 (MQ=255)

            gCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGt                                                                                                                                          <  1:3484319/150‑1 (MQ=255)

             cccAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTa                                                                                                                                         >  2:3832984/1‑150 (MQ=255)

              ccAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTAc                                                                                                                                        <  2:1774636/150‑1 (MQ=255)

              ccAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTAc                                                                                                                                        <  2:2368626/150‑1 (MQ=255)

                aGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCt                                                                                                                                      <  2:4399851/150‑1 (MQ=255)

                 gATTTCCGACGATATCATGGAAAACCTCGGCTAAGGTTATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTg                                                                                                                                     <  1:500561/150‑1 (MQ=255)

                 gATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTg                                                                                                                                     <  1:2998880/150‑1 (MQ=255)

                    ttCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGTTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAg                                                                                                                                  <  1:1103546/150‑1 (MQ=255)

                    ttCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAg                                                                                                                                  <  1:3211052/150‑1 (MQ=255)

                    ttCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAg                                                                                                                                  <  2:3786896/150‑1 (MQ=255)

                     tCCGCCGAGATCATGGAACACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAgg                                                                                                                                 <  1:1916314/150‑1 (MQ=18)

                     tCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAgg                                                                                                                                 <  1:739310/150‑1 (MQ=18)

                     tCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAgg                                                                                                                                 <  1:1916310/150‑1 (MQ=18)

                     tCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAgg                                                                                                                                 <  1:1916230/150‑1 (MQ=18)

                     tCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAgg                                                                                                                                 <  1:2755680/150‑1 (MQ=18)

                      ccGACGAGATCATGGAAACCCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGCGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAGGa                                                                                                                                <  1:285505/150‑1 (MQ=17)

                      ccGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGa                                                                                                                                <  1:2618769/150‑1 (MQ=255)

                       cgacgaGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCGGTAGGAg                                                                                                                               >  2:2081676/1‑150 (MQ=255)

                       cgacgaGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAGGAg                                                                                                                               <  2:2454211/150‑1 (MQ=18)

                        gacgaGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGc                                                                                                                              <  1:4128711/150‑1 (MQ=255)

                        gacgaGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGc                                                                                                                              <  2:3642651/150‑1 (MQ=255)

                        gacgaGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGc                                                                                                                              <  1:973625/150‑1 (MQ=255)

                         acgaGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑g                                                                                                                            <  2:303805/150‑1 (MQ=255)

                          cgaGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑gg                                                                                                                           <  1:2688420/150‑1 (MQ=255)

                          cgaGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑gg                                                                                                                           <  1:372107/150‑1 (MQ=255)

                          cgaGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑gg                                                                                                                           <  2:4160555/150‑1 (MQ=255)

                          cgaGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGCGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAGGAGC‑gg                                                                                                                           <  2:3769324/150‑1 (MQ=17)

                           gagaTCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGCTAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTCCAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑ggg                                                                                                                          <  2:4005132/150‑1 (MQ=255)

                           gagaTCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑ggg                                                                                                                          <  2:3629026/150‑1 (MQ=255)

                             gaTCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCCCGCCAACACGTATTAAG‑CC‑CCAATAAAAAGTGGTGCAGGACCGGGAGGGGC‑ggggt                                                                                                                        >  2:1907814/1‑148 (MQ=255)

                              aTCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGATTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGttt                                                                                                                       <  1:2024226/150‑1 (MQ=255)

                                cATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTAc                                                                                                                     <  1:490568/150‑1 (MQ=255)

                                 aTGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCGTTCGCGGGTAAAACCCCACCGACAGGTATTACG‑CC‑CAATTAAAATGTGGTGGCGTACCGGTGGGAGG‑GGGTTTaac                                                                                                                    >  2:2667444/1‑148 (MQ=255)

                                   ggAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTTAAACCCCGCCGACAGGGATTAAG‑CC‑CATTTGAAATGTGGGGGAGTAACGGTGTGAGCGGGGTTTAccg                                                                                                                   >  2:1554695/1‑149 (MQ=255)

                                   ggAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAGGAGC‑GGGTTTACCcg                                                                                                                  <  1:3052465/150‑1 (MQ=17)

                                   ggAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACACCCTCCAACAGGTATTAAGCCC‑CATTACAAA‑GGGGGGCCGTACCAGTAGGAGC‑GGGTTTAACcg                                                                                                                  >  2:1554687/1‑150 (MQ=37)

                                   ggAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGGAAACCCGCGCCGACAGGTATTACG‑CC‑CCTTTCCAATTTGGTGGAGTACCGGGTGGTGC‑GGGTTTagacg                                                                                                                  >  1:2218242/1‑146 (MQ=255)

                                    gAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAGGAGC‑GGGTTTACCcgc                                                                                                                 <  2:3196375/150‑1 (MQ=17)

                                         ccTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAAACCGCGCCAACAGGTATTACG‑CC‑CATTTCAAATGTGGGGCAGTACCGGTAGGAGC‑GGGTTTACCCGCGAAAg                                                                                                            >  2:711280/1‑150 (MQ=38)

                                           tCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCCCAACAACAGGTATTACG‑CC‑CCATTCAAATGGGGTGCCGTACCGGTAGGGGC‑GGGTTGAACCGCGCAAggg                                                                                                          >  2:172372/1‑150 (MQ=255)

                                              gCTATGGTGATTGGGAATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCGGTGCGGGCCCTTTCGCGGGTAACCCCGCTCCTACAGGTATTACG‑CC‑AATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGTTTTACCCGCGAAAGGGCCg                                                                                                       <  2:2154507/150‑1 (MQ=37)

                                              gCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTTAAACCCCGCCAACCGGTATTACG‑CC‑CCTTTACAATGTGGTGGCGTACCGGTAGGAGC‑GGGGTTACCCGCGCCAGGGCCg                                                                                                       >  2:3779086/1‑150 (MQ=255)

                                                        tttGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGGAAACCCCCGCCTACAGGTATTAAG‑CC‑CAATTCAAATGTGGTGCAGTTCCTGTTGGGGC‑GGGGTTACCCGCGGAACGGGCGGGGCCGGTTg                                                                                             >  2:1948853/1‑150 (MQ=255)

                                                                    tgCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCCCTCCAACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCCCGAAAGGGCCGGCGCAGGTTGTAGAGATGcaac                                                                                 >  2:4033761/1‑148 (MQ=255)

                                                                    tgCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAAACCGCTACAACAGGTATTAAG‑CC‑CATTTAAAATGTTGTGGAGTACATGTAGGAGC‑GGGTTTAACCGCGAAAGGGCCGGCGCAGGTTGTAGAGATTcaaa                                                                                 >  2:238531/1‑148 (MQ=25)

                                                                      ccAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAg                                                                                                                                  <  1:291253/100‑1 (MQ=255)

                                                                             tGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTGAAAACCCGCCAACACGTAGTACG‑CC‑CCATTCAAAGGTGGTGGCGTACCGGTTGGGGC‑GGGGGTTCCCCCGCAAGGGCCCGCGCCGGGTGTTGAGGTGGAACCttttttgg                                                                        >  2:843466/1‑148 (MQ=255)

                                                                                  gTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAAACCGCGCCTAAACGGCTTAAG‑CC‑CAGTTAAAATGTGGTGCCGGACCGGTAGGAGC‑GGGTTTAACCGCGCAAGGGCCGGCGCGGGGTGGAGAGATGCCTGATTTTTTCGACggg                                                                   >  2:3095040/1‑150 (MQ=255)

                                                                                       aTAGCGCCTGTGCCGGCCCATTCGCGGGTAAAACCGCACCAAAAGGTGTTACC‑CC‑CCATTCAAAACTGGGGCCGTACCGGTGGGAGC‑GGGGTTACCCCCGCAAGGGCCGGCGCCGTTTGGAGCGGAGCCACATTTTTTAGAAGGGCtttt                                                              >  2:3128043/1‑149 (MQ=255)

                                                                                               tgtgCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑gg                                                                                                                           <  2:508792/81‑1 (MQ=255)

                                                                                                    cGGCCCTTTCGGGGCAAAACCCGCACCTCCTGTTATTACC‑CC‑CTTTTCACTTGTGGTGCCTAACCTTTCGGCGG‑GGTTTAACCCGCGAACGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCt                                                 <  2:1990483/150‑1 (MQ=255)

                                                                                                          tttCGCGGGTAAACCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGGTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTAt                                           >  2:2789321/1‑150 (MQ=255)

                                                                                                            tCGCGGGTAAACCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCCGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCg                                         >  2:266095/1‑150 (MQ=255)

                                                                                                              gcgGGTAAACCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTAACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGAc                                       >  2:364614/1‑150 (MQ=255)

                                                                                                                  taaaaCCCGCTCCTACTGTTATTACG‑CC‑AATTTCAATTGTGGTGCTGTACCTGTTGGTGG‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCg                                   <  2:2218242/148‑1 (MQ=255)

                                                                                                                   tAAACCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCgg                                  >  1:4235469/1‑150 (MQ=255)

                                                                                                                      ccccGCGCCGACAGGTATTCCG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGG‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTc                               <  2:2625821/149‑1 (MQ=255)

                                                                                                                      aCCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTc                               >  1:176582/1‑150 (MQ=255)

                                                                                                                       cccGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTcg                              >  1:3045565/1‑150 (MQ=255)

                                                                                                                       cccGCTCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTcg                              >  1:561863/1‑150 (MQ=18)

                                                                                                                        ccGCTCCTACAGGTATTACG‑CC‑CATTTCACATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGGTTGTAGAGATGCATCATTTTTTCGACGGGCGTTATGACTCTGATTCTAGGTATCGACCCCGGCTcgc                             >  2:3996063/1‑150 (MQ=255)

                                                                                                                        ccGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGCGCTGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTcgc                             >  2:3032821/1‑150 (MQ=255)

                                                                                                                        ccGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTcgc                             >  1:4409362/1‑150 (MQ=255)

                                                                                                                        ccGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTcgc                             >  1:3331845/1‑150 (MQ=255)

                                                                                                                           cTCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCa                          >  1:3821816/1‑150 (MQ=18)

                                                                                                                           cTCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCa                          >  1:3821846/1‑150 (MQ=18)

                                                                                                                            tCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGa                                                                                                                                <  1:322888/48‑1 (MQ=255)

                                                                                                                            tCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGa                                                                                                                                >  2:322888/1‑48 (MQ=255)

                                                                                                                            tCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTGTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGGATCGACCCCGGCTCGCGCAt                         >  1:2026848/1‑150 (MQ=18)

                                                                                                                            tCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCACAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCAt                         >  2:4261762/1‑150 (MQ=18)

                                                                                                                             ccTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATc                        >  1:3565190/1‑150 (MQ=255)

                                                                                                                               tACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCAc                      >  2:393620/1‑150 (MQ=255)

                                                                                                                                   ggTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGc                  >  1:1038516/1‑150 (MQ=255)

                                                                                                                                    gTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCt                 >  1:3694618/1‑150 (MQ=255)

                                                                                                                                    gTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCt                 >  1:2511448/1‑150 (MQ=255)

                                                                                                                                           g‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGt          >  2:2821119/1‑150 (MQ=255)

                                                                                                                                           g‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGt          >  1:1821022/1‑150 (MQ=255)

                                                                                                                                           g‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGt          >  2:3719808/1‑150 (MQ=255)

                                                                                                                                           g‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGt          >  1:2877975/1‑150 (MQ=255)

                                                                                                                                              c‑cATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTgg        >  2:1577085/1‑150 (MQ=255)

                                                                                                                                              c‑cATTTCAAAGGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTgg        >  2:144029/1‑150 (MQ=255)

                                                                                                                                                cATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGt       >  1:1197523/1‑150 (MQ=255)

                                                                                                                                                cATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGt       >  2:1997444/1‑150 (MQ=255)

                                                                                                                                                 aTTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGTa      >  2:3303934/1‑150 (MQ=255)

                                                                                                                                                 aTTTCAAATGTGGTGCAGTACCTGTAAGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGTa      >  2:325698/1‑150 (MQ=255)

                                                                                                                                                   ttCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGTACg    >  1:2240641/1‑150 (MQ=255)

GTCTATTCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGTACGCC  >  NC_002947/1392786‑1393082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`