Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,392,942 T→C 17.2% intergenic (+106/‑80) PP_1214 → / → ruvC transcriptional regulator/crossover junction endodeoxyribonuclease RuvC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,392,9420TC17.2% 314.4 / 50.7 129intergenic (+106/‑80)PP_1214/ruvCtranscriptional regulator/crossover junction endodeoxyribonuclease RuvC
Reads supporting (aligned to +/- strand):  ref base T (61/42);  new base C (8/14);  total (73/56)
Fisher's exact test for biased strand distribution p-value = 6.09e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.79e-01

Only 100 of 138 total aligned reads displayed.

CCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGTACGCCAGACCGCC  >  NC_002947/1392793‑1393090
                                                                                                                                                         |                                                                                                                                                    
ccAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATgtggt                                                                                                                                                      <  2:896129/150‑1 (MQ=255)
ccAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATgtggt                                                                                                                                                      <  1:1585910/150‑1 (MQ=255)
 cAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATgtggtg                                                                                                                                                     <  2:4363537/150‑1 (MQ=255)
 cAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATgtggtg                                                                                                                                                     <  2:1885807/150‑1 (MQ=255)
 cAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATgtggtg                                                                                                                                                     <  2:124268/150‑1 (MQ=255)
 cAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCGAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATgtggtg                                                                                                                                                     <  2:124308/150‑1 (MQ=255)
      cccAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTa                                                                                                                                                >  2:3832984/1‑150 (MQ=255)
       ccAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTAc                                                                                                                                               <  2:2368626/150‑1 (MQ=255)
       ccAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTAc                                                                                                                                               <  2:1774636/150‑1 (MQ=255)
         aGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCt                                                                                                                                             <  2:4399851/150‑1 (MQ=255)
         aGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCt                                                                                                                                             <  2:2217255/150‑1 (MQ=255)
          gATTTCCGACGATATCATGGAAAACCTCGGCTAAGGTTATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTg                                                                                                                                            <  1:500561/150‑1 (MQ=255)
          gATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTg                                                                                                                                            <  1:2998880/150‑1 (MQ=255)
           aTTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGt                                                                                                                                           <  1:1502852/150‑1 (MQ=21)
             ttCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGTTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAg                                                                                                                                         <  1:1103546/150‑1 (MQ=255)
             ttCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAg                                                                                                                                         <  1:3211052/150‑1 (MQ=255)
              tCCGCCGAGATCATGGAACACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAgg                                                                                                                                        <  1:1916314/150‑1 (MQ=18)
              tCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAgg                                                                                                                                        <  1:1916230/150‑1 (MQ=18)
              tCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAgg                                                                                                                                        <  1:1916310/150‑1 (MQ=18)
              tCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAgg                                                                                                                                        <  1:739310/150‑1 (MQ=18)
               ccGACGAGATCATGGAAACCCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGCGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAGGa                                                                                                                                       <  1:285505/150‑1 (MQ=17)
               ccGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGa                                                                                                                                       <  1:2618769/150‑1 (MQ=255)
               ccGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGa                                                                                                                                       <  1:361716/150‑1 (MQ=255)
                cgacgaGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCGGTAGGAg                                                                                                                                      >  2:2081676/1‑150 (MQ=255)
                cgacgaGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAGGAg                                                                                                                                      <  2:2454211/150‑1 (MQ=18)
                 gacgaGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGc                                                                                                                                     <  2:3642651/150‑1 (MQ=255)
                 gacgaGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGc                                                                                                                                     <  1:973625/150‑1 (MQ=255)
                  acgaGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCg                                                                                                                                    <  2:303805/150‑1 (MQ=255)
                   cgaGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCgg                                                                                                                                   <  1:2688420/150‑1 (MQ=255)
                   cgaGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCgg                                                                                                                                   <  1:2074801/150‑1 (MQ=255)
                   cgaGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCgg                                                                                                                                   <  2:4160555/150‑1 (MQ=255)
                   cgaGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCgg                                                                                                                                   <  1:372196/150‑1 (MQ=255)
                   cgaGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGCGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAGGAGCgg                                                                                                                                   <  2:3769324/150‑1 (MQ=17)
                    gagaTCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGCTAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTCCAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCggg                                                                                                                                  <  2:4005132/150‑1 (MQ=255)
                    gagaTCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCggg                                                                                                                                  <  2:4002427/150‑1 (MQ=255)
                      gaTCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCCCGCCAACACGTATTAAG‑CC‑CCAATAAAAAGTGGTGCAGGACCGGGAGGGGCggggt                                                                                                                                >  2:1907814/1‑148 (MQ=255)
                      gaTCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCATTCGC‑GGGTAAA‑CCCCCGCCTACAGGTATTACG‑CC‑CAATTAAAAAGTGGTGCAGTACCAGGAGGGGCggggt                                                                                                                                >  2:1907894/1‑148 (MQ=255)
                       aTCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGATTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGttt                                                                                                                               <  1:2024226/150‑1 (MQ=255)
                         cATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTAc                                                                                                                             <  1:490568/150‑1 (MQ=255)
                          aTGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCGTTCGC‑GGGTAAA‑ACCCCACCGACAGGTATTACG‑CC‑CAATTAAAATGTGGTGGCGTACCGGTGGGAGGGGGTTTaac                                                                                                                            >  2:2667444/1‑148 (MQ=255)
                            ggAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CACCCTCCAACAGGTATTAAGCCC‑CATTACAAA‑GGGGGGCCGTACCAGTAGGAGCGGGTTTAACcg                                                                                                                          >  2:1554687/1‑150 (MQ=37)
                             gAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCcgc                                                                                                                         <  1:2732012/150‑1 (MQ=255)
                             gAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAGGAGCGGGTTTACCcgc                                                                                                                         <  2:3196375/150‑1 (MQ=17)
                                  ccTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑ACCGCGCCAACAGGTATTACG‑CC‑CATTTCAAATGTGGGGCAGTACCGGTAGGAGCGGGTTTACCCGCGAAAg                                                                                                                    >  2:711280/1‑150 (MQ=38)
                                    tCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCCCAACAACAGGTATTACG‑CC‑CCATTCAAATGGGGTGCCGTACCGGTAGGGGCGGGTTGAACCGCGCAAggg                                                                                                                  >  2:172372/1‑150 (MQ=255)
                                     cGGCTAAGGTGATTGGCCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTCCGC‑GGGTAAA‑CCCGCTCCTACTGGTATTCCG‑CC‑CATTTCAACTGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGc                                                                                                                 <  2:3850003/150‑1 (MQ=37)
                                       gCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTTAA‑ACCCCGCCAACCGGTATTACG‑CC‑CCTTTACAATGTGGTGGCGTACCGGTAGGAGCGGGGTTACCCGCGCCAGGGCCg                                                                                                               >  2:3779086/1‑150 (MQ=255)
                                       gCTAAGGTGATTTGGCAGTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGGTAAA‑CCC‑CACCGACCGGGATTAAG‑CC‑CCATTCAAATGTGGTGCGCTACCGGGTGGAGGGGGGTTGCCCCCGACAAGGCCg                                                                                                               >  2:3779046/1‑150 (MQ=255)
                                                             tgCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑ACCGCTACAACAGGTATTAAG‑CC‑CATTTAAAATGTTGTGGAGTACATGTAGGAGCGGGTTTAACCGCGAAAGGGCCGGCGCAGGTTGTAGAGATTcaaa                                                                                         >  2:238531/1‑148 (MQ=25)
                                                               ccAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAg                                                                                                                                         >  2:291253/1‑100 (MQ=255)
                                                               ccAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAg                                                                                                                                         <  1:291253/100‑1 (MQ=255)
                                                                 aaCTCTGTGCGTTGGATAGCGCGTGGGCCGCCCTTTTCG‑‑GGTTAAACCCCCCCCCCCCTGGTTTGCCG‑CC‑ATTTTCAATTTTGGTGCTGTACCGTTAGGTGGGGTTTGCCCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCAttt                                                                                     <  2:3223686/150‑1 (MQ=255)
                                                                      tGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTGAA‑AACCCGCCAACACGTAGTACG‑CC‑CCATTCAAAGGTGGTGGCGTACCGGTTGGGGCGGGGGTTCCCCCGCAAGGGCCCGCGCCGGGTGTTGAGGTGGAACCttttttgg                                                                                >  2:843466/1‑148 (MQ=255)
                                                                           gTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑ACCGCGCCTAAACGGCTTAAG‑CC‑CAGTTAAAATGTGGTGCCGGACCGGTAGGAGCGGGTTTAACCGCGCAAGGGCCGGCGCGGGGTGGAGAGATGCCTGATTTTTTCGACggg                                                                           >  2:3095040/1‑150 (MQ=255)
                                                                                aTAGCGCCTGTGCCGGCCCATTCGC‑GGGTAAA‑ACCGCACCAAAAGGTGTTACC‑CC‑CCATTCAAAACTGGGGCCGTACCGGTGGGAGCGGGGTTACCCCCGCAAGGGCCGGCGCCGTTTGGAGCGGAGCCACATTTTTTAGAAGGGCtttt                                                                      >  2:3128043/1‑149 (MQ=255)
                                                                                        tgtgCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCgg                                                                                                                                   <  2:508792/81‑1 (MQ=255)
                                                                                             cGGCCCTTTCGG‑GGCAAAA‑CCCGCACCTCCTGTTATTACC‑CC‑CTTTTCACTTGTGGTGCCTAACCTTTCGGCGGGGTTTAACCCGCGAACGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCt                                                         <  2:1990483/150‑1 (MQ=255)
                                                                                                  cTTTCGC‑GGGTAAA‑ACCGCTCCTACAGGTATTAAG‑CC‑CCTTTCAAATGTGGTGCAGTACCTGTATGAGCGGGTTTAACCGCGAAAGGGCCGGCGCAGTTTTTAGAGATGCAACAATTTTTATAAGGGCTTTATTAATCTGATTCTATGTa                                                    >  2:2073681/1‑150 (MQ=255)
                                                                                                   tttCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGGTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTAt                                                   >  2:2789321/1‑150 (MQ=255)
                                                                                                     tCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCCGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCg                                                 >  2:266095/1‑150 (MQ=255)
                                                                                                       gc‑gGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTAACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGAc                                               >  2:364614/1‑150 (MQ=255)
                                                                                                            taaaa‑CCCGCTCCTACTGTTATTACG‑CC‑AATTTCAATTGTGGTGCTGTACCTGTTGGTGGGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCg                                           <  2:2218242/148‑1 (MQ=255)
                                                                                                             tAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCgg                                          >  1:4235469/1‑150 (MQ=255)
                                                                                                                a‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTc                                       >  1:176582/1‑150 (MQ=255)
                                                                                                                 ccccGCGCCGACAGGTATTCCG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGGGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTc                                       <  2:2625821/149‑1 (MQ=255)
                                                                                                                  cccGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTcg                                      >  1:3045565/1‑150 (MQ=255)
                                                                                                                  cccGCTCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTcg                                      >  1:561863/1‑150 (MQ=18)
                                                                                                                   ccGCTCCTACAGGTATTACG‑CC‑CATTTCACATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGGTTGTAGAGATGCATCATTTTTTCGACGGGCGTTATGACTCTGATTCTAGGTATCGACCCCGGCTcgc                                     >  2:3996063/1‑150 (MQ=255)
                                                                                                                   ccGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTcgc                                     >  1:3061460/1‑150 (MQ=255)
                                                                                                                   ccGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTcgc                                     >  1:3331845/1‑150 (MQ=255)
                                                                                                                     gCTCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCgcgc                                   >  2:478922/1‑150 (MQ=18)
                                                                                                                     gCTCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCgcgc                                   >  2:1620178/1‑150 (MQ=18)
                                                                                                                      cTCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCa                                  >  1:3821846/1‑150 (MQ=18)
                                                                                                                      cTCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCa                                  >  1:3821816/1‑150 (MQ=18)
                                                                                                                       tCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGa                                                                                                                                       <  1:322888/48‑1 (MQ=255)
                                                                                                                       tCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTGTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGGATCGACCCCGGCTCGCGCAt                                 >  1:2026848/1‑150 (MQ=18)
                                                                                                                       tCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCACAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCAt                                 >  2:4261762/1‑150 (MQ=18)
                                                                                                                        ccTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATc                                >  1:3565190/1‑150 (MQ=255)
                                                                                                                          tACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGGAGAGATGCATCATTTTTTCGACGGGCGTTATGACTCTGAGTCTAGGTATCGACCCCGGCTCGCGCATCAc                              >  2:4124303/1‑150 (MQ=255)
                                                                                                                              ggTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGc                          >  1:1038516/1‑150 (MQ=255)
                                                                                                                               gTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCt                         >  1:3694618/1‑150 (MQ=255)
                                                                                                                               gTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCt                         >  2:1488795/1‑150 (MQ=255)
                                                                                                                                    aCG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGc                    >  1:241693/1‑150 (MQ=255)
                                                                                                                                      g‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGt                  >  1:2877975/1‑150 (MQ=255)
                                                                                                                                      g‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGt                  >  2:2821119/1‑150 (MQ=255)
                                                                                                                                      g‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGt                  >  1:1821022/1‑150 (MQ=255)
                                                                                                                                      g‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGt                  >  1:479090/1‑150 (MQ=255)
                                                                                                                                        ccacATTTGAAATG‑GGCGTAATACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCACAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTg                 <  1:4261762/147‑1 (MQ=25)
                                                                                                                                         c‑cATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTgg                >  2:1577085/1‑150 (MQ=255)
                                                                                                                                         c‑cATTTCAAAGGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTgg                >  2:144029/1‑150 (MQ=255)
                                                                                                                                           cATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGt               >  2:1997444/1‑150 (MQ=255)
                                                                                                                                           cATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGt               >  1:1197523/1‑150 (MQ=255)
                                                                                                                                            aTTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGTa              >  2:3303934/1‑150 (MQ=255)
                                                                                                                                            aTTTCAAATGTGGTGCAGTACCTGTAAGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGTa              >  2:325698/1‑150 (MQ=255)
                                                                                                                                             tttCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGTAc             >  2:3643439/1‑150 (MQ=255)
                                                                                                                                              ttCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGTACg            >  1:2240641/1‑150 (MQ=255)
                                                                                                                                                cAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGTACGcc          >  1:3464222/1‑150 (MQ=255)
                                                                                                                                                 aaaTGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACACCGGCTCGCGCATCACCGGCTATGGCGTGGTACGCCa         >  2:171393/1‑150 (MQ=255)
                                                                                                                                                   aTGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGTACGCCAGa       <  1:1577085/150‑1 (MQ=255)
                                                                                                                                                        gtgCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCCGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGTACGCCAGgccgcc  >  2:2019929/1‑150 (MQ=255)
                                                                                                                                                         |                                                                                                                                                    
CCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGC‑GGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGTACGCCAGACCGCC  >  NC_002947/1392793‑1393090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑