Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,392,946 G→A 16.8% intergenic (+110/‑76) PP_1214 → / → ruvC transcriptional regulator/crossover junction endodeoxyribonuclease RuvC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,392,9460GA16.8% 322.1 / 48.8 133intergenic (+110/‑76)PP_1214/ruvCtranscriptional regulator/crossover junction endodeoxyribonuclease RuvC
Reads supporting (aligned to +/- strand):  ref base G (68/41);  new base A (8/14);  total (77/56)
Fisher's exact test for biased strand distribution p-value = 3.27e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 136 total aligned reads displayed.

GCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGTACGCCAGACCGCCCGTGG  >  NC_002947/1392798‑1393095
                                                                                                                                                       |                                                                                                                                                      
gCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGt                                                                                                                                                       <  1:3484319/150‑1 (MQ=255)
 cccAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTa                                                                                                                                                      >  2:3832984/1‑150 (MQ=255)
  ccAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTAc                                                                                                                                                     <  2:1774636/150‑1 (MQ=255)
  ccAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTAc                                                                                                                                                     <  2:2368626/150‑1 (MQ=255)
  ccAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTAc                                                                                                                                                     <  2:2368662/150‑1 (MQ=255)
    aGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCt                                                                                                                                                   <  2:4399851/150‑1 (MQ=255)
    aGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCt                                                                                                                                                   <  2:2217255/150‑1 (MQ=255)
     gATTTCCGACGATATCATGGAAAACCTCGGCTAAGGTTATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTg                                                                                                                                                  <  1:500561/150‑1 (MQ=255)
     gATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTg                                                                                                                                                  <  1:2998880/150‑1 (MQ=255)
      aTTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAA‑CCCGCTCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGt                                                                                                                                                 <  2:1016199/150‑1 (MQ=21)
      aTTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAA‑CCCGCTCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGt                                                                                                                                                 <  1:1502852/150‑1 (MQ=21)
        ttCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAA‑CCCGCTCCTACAGTTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAg                                                                                                                                               <  1:1103546/150‑1 (MQ=255)
        ttCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAg                                                                                                                                               <  2:3786896/150‑1 (MQ=255)
        ttCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAg                                                                                                                                               <  1:3281105/150‑1 (MQ=255)
         tCCGCCGAGATCATGGAACACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAA‑CCCGCTCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAgg                                                                                                                                              <  1:1916314/150‑1 (MQ=18)
         tCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAA‑CCCGCTCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAgg                                                                                                                                              <  1:1916230/150‑1 (MQ=18)
         tCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAA‑CCCGCTCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAgg                                                                                                                                              <  1:2755680/150‑1 (MQ=18)
          ccGACGAGATCATGGAAACCCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGCGCCGGCCCTTTCGCGGGTAAA‑CCCGCTCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAGGa                                                                                                                                             <  1:285505/150‑1 (MQ=17)
          ccGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGa                                                                                                                                             <  1:2618769/150‑1 (MQ=255)
           cgacgaGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCGGTAGGAg                                                                                                                                            >  2:2081676/1‑150 (MQ=255)
           cgacgaGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAA‑CCCGCTCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAGGAg                                                                                                                                            <  2:2454211/150‑1 (MQ=18)
            gacgaGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGc                                                                                                                                           <  2:3642651/150‑1 (MQ=255)
            gacgaGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGc                                                                                                                                           <  1:4128711/150‑1 (MQ=255)
            gacgaGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGc                                                                                                                                           <  1:973625/150‑1 (MQ=255)
             acgaGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑g                                                                                                                                         <  2:303805/150‑1 (MQ=255)
              cgaGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑gg                                                                                                                                        <  1:372107/150‑1 (MQ=255)
              cgaGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑gg                                                                                                                                        <  1:2074801/150‑1 (MQ=255)
              cgaGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑gg                                                                                                                                        <  1:372196/150‑1 (MQ=255)
              cgaGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGCGCCGGCCCTTTCGCGGGTAAA‑CCCGCTCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAGGAGC‑gg                                                                                                                                        <  2:3769324/150‑1 (MQ=17)
               gagaTCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGCTAGCGCCTGTGCCGGCCCTTTCGCGGGTAAA‑CCCGCTCCTCCAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑ggg                                                                                                                                       <  2:4005132/150‑1 (MQ=255)
               gagaTCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑ggg                                                                                                                                       <  2:3629026/150‑1 (MQ=255)
                agaTCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAA‑CCCGCTCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAGGAGC‑GGGt                                                                                                                                      <  2:4355879/150‑1 (MQ=18)
                 gaTCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAA‑CCCCCGCCAACACGTATTAAG‑CC‑CCAATAAAAAGTGGTGCAGGACCGGGAGGGGC‑ggggt                                                                                                                                     >  2:1907814/1‑148 (MQ=255)
                 gaTCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCATTCGCGGGTAAA‑CCCCCGCCTACAGGTATTACG‑CC‑CAATTAAAAAGTGGTGCAGTACCAGGAGGGGC‑ggggt                                                                                                                                     >  2:1907894/1‑148 (MQ=255)
                  aTCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGATTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGttt                                                                                                                                    <  1:2024226/150‑1 (MQ=255)
                     aTGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CAATTGAAATGTTGTGGCGTACCTGTAGGAGC‑GGGTTTAcc                                                                                                                                 >  2:2667504/1‑150 (MQ=38)
                     aTGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCGTTCGCGGGTAAA‑ACCCCACCGACAGGTATTACG‑CC‑CAATTAAAATGTGGTGGCGTACCGGTGGGAGG‑GGGTTTaac                                                                                                                                 >  2:2667444/1‑148 (MQ=255)
                       ggAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTTAA‑ACCCCGCCGACAGGGATTAAG‑CC‑CATTTGAAATGTGGGGGAGTAACGGTGTGAGCGGGGTTTAccg                                                                                                                                >  2:1554695/1‑149 (MQ=255)
                       ggAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAA‑CCCGCTCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAGGAGC‑GGGTTTACCcg                                                                                                                               <  1:3052465/150‑1 (MQ=17)
                       ggAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAA‑CACCCTCCAACAGGTATTAAGCCC‑CATTACAAA‑GGGGGGCCGTACCAGTAGGAGC‑GGGTTTAACcg                                                                                                                               >  2:1554687/1‑150 (MQ=37)
                       ggAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGGAAA‑CCCGCGCCGACAGGTATTACG‑CC‑CCTTTCCAATTTGGTGGAGTACCGGGTGGTGC‑GGGTTTagacg                                                                                                                               >  1:2218242/1‑146 (MQ=255)
                        gAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCcgc                                                                                                                              <  1:2732012/150‑1 (MQ=255)
                            aCCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGaaa                                                                                                                          <  2:1411519/150‑1 (MQ=255)
                               tCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAA‑CCCCCAACAACAGGTATTACG‑CC‑CCATTCAAATGGGGTGCCGTACCGGTAGGGGC‑GGGTTGAACCGCGCAAggg                                                                                                                       >  2:172372/1‑150 (MQ=255)
                                  gCTATGGTGATTGGGAATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCGGTGCGGGCCCTTTCGCGGGTAAC‑CCCGCTCCTACAGGTATTACG‑CC‑AATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGTTTTACCCGCGAAAGGGCCg                                                                                                                    <  2:2154507/150‑1 (MQ=37)
                                  gCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTTAA‑ACCCCGCCAACCGGTATTACG‑CC‑CCTTTACAATGTGGTGGCGTACCGGTAGGAGC‑GGGGTTACCCGCGCCAGGGCCg                                                                                                                    >  2:3779086/1‑150 (MQ=255)
                                            tttGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGGAAA‑CCCCCGCCTACAGGTATTAAG‑CC‑CAATTCAAATGTGGTGCAGTTCCTGTTGGGGC‑GGGGTTACCCGCGGAACGGGCGGGGCCGGTTg                                                                                                          >  2:1948853/1‑150 (MQ=255)
                                                        tgCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAA‑CCCCCTCCAACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCCCGAAAGGGCCGGCGCAGGTTGTAGAGATGcaac                                                                                              >  2:4033761/1‑148 (MQ=255)
                                                          ccAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAg                                                                                                                                               <  1:291253/100‑1 (MQ=255)
                                                          ccAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAg                                                                                                                                               >  2:291253/1‑100 (MQ=255)
                                                            aaCTCTGTGCGTTGGATAGCGCGTGGGCCGCCCTTTTCG‑GGTTAAACCCCCCCCCCCCTGGTTTGCCG‑CC‑ATTTTCAATTTTGGTGCTGTACCGTTAGGTGG‑GGTTTGCCCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCAttt                                                                                          <  2:3223686/150‑1 (MQ=255)
                                                                 tGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTGAA‑AACCCGCCAACACGTAGTACG‑CC‑CCATTCAAAGGTGGTGGCGTACCGGTTGGGGC‑GGGGGTTCCCCCGCAAGGGCCCGCGCCGGGTGTTGAGGTGGAACCttttttgg                                                                                     >  2:843466/1‑148 (MQ=255)
                                                                           aTAGCGCCTGTGCCGGCCCATTCGCGGGTAAA‑ACCGCACCAAAAGGTGTTACC‑CC‑CCATTCAAAACTGGGGCCGTACCGGTGGGAGC‑GGGGTTACCCCCGCAAGGGCCGGCGCCGTTTGGAGCGGAGCCACATTTTTTAGAAGGGCtttt                                                                           >  2:3128043/1‑149 (MQ=255)
                                                                                   tgtgCCGGCCCTTTCGCGGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑gg                                                                                                                                        <  2:508792/81‑1 (MQ=255)
                                                                                   tgtgCCGGCCCTTTCGCGGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑gg                                                                                                                                        >  1:508792/1‑81 (MQ=255)
                                                                                             cTTTCGCGGGTAAA‑ACCGCTCCTACAGGTATTAAG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTAACCGCGAAAGGGCCGGCGCAGTTTGTATAGATGCATCAATTTTTCGACGGGCTTTATTACTATGATTCTAGGTa                                                         >  2:3124403/1‑150 (MQ=37)
                                                                                              tttCGCGGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGGTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTAt                                                        >  2:2789321/1‑150 (MQ=255)
                                                                                                  gcgGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTAACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGAc                                                    >  2:364614/1‑150 (MQ=255)
                                                                                                       tAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCgg                                               >  1:4235469/1‑150 (MQ=255)
                                                                                                         aa‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCt                                             >  2:744205/1‑150 (MQ=255)
                                                                                                           ccccGCGCCGACAGGTATTCCG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGG‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTc                                            <  2:2625821/149‑1 (MQ=255)
                                                                                                            cccGCTCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTcg                                           >  1:561863/1‑150 (MQ=18)
                                                                                                             ccGCTCCTACAGGTATTACG‑CC‑CATTTCACATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGGTTGTAGAGATGCATCATTTTTTCGACGGGCGTTATGACTCTGATTCTAGGTATCGACCCCGGCTcgc                                          >  2:3996063/1‑150 (MQ=255)
                                                                                                             ccGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGCGCTGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTcgc                                          >  2:3032821/1‑150 (MQ=255)
                                                                                                             ccGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTcgc                                          >  1:3331845/1‑150 (MQ=255)
                                                                                                             ccGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTcgc                                          >  1:4409362/1‑150 (MQ=255)
                                                                                                             ccGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTcgc                                          >  1:3061460/1‑150 (MQ=255)
                                                                                                               gCTCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCgcgc                                        >  2:478922/1‑150 (MQ=18)
                                                                                                               gCTCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCgcgc                                        >  2:1620178/1‑150 (MQ=18)
                                                                                                                cTCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCa                                       >  1:3821846/1‑150 (MQ=18)
                                                                                                                 tCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGa                                                                                                                                             <  1:322888/48‑1 (MQ=255)
                                                                                                                 tCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGa                                                                                                                                             >  2:322888/1‑48 (MQ=255)
                                                                                                                 tCCTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCACAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCAt                                      >  2:4261762/1‑150 (MQ=18)
                                                                                                                  ccTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATc                                     >  1:3565190/1‑150 (MQ=255)
                                                                                                                   cTACAGGTACTGCA‑CCACATTTGAAATG‑GGCGTAATACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTCGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCa                                    >  1:1897715/1‑150 (MQ=18)
                                                                                                                    tACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCAc                                   >  2:393620/1‑150 (MQ=255)
                                                                                                                    tACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGGAGAGATGCATCATTTTTTCGACGGGCGTTATGACTCTGAGTCTAGGTATCGACCCCGGCTCGCGCATCAc                                   >  2:4124303/1‑150 (MQ=255)
                                                                                                                       aGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCgg                                >  1:2995928/1‑150 (MQ=255)
                                                                                                                        ggTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGc                               >  1:1038516/1‑150 (MQ=255)
                                                                                                                         gTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCt                              >  1:3694618/1‑150 (MQ=255)
                                                                                                                         gTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCt                              >  2:1488795/1‑150 (MQ=255)
                                                                                                                         gTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCt                              >  1:2511448/1‑150 (MQ=255)
                                                                                                                              aCG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGc                         >  1:241693/1‑150 (MQ=255)
                                                                                                                                g‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGt                       >  1:1821022/1‑150 (MQ=255)
                                                                                                                                g‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGt                       >  1:1941954/1‑150 (MQ=255)
                                                                                                                                g‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGt                       >  2:3719808/1‑150 (MQ=255)
                                                                                                                                g‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGt                       >  1:479090/1‑150 (MQ=255)
                                                                                                                                     cATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGt                    >  2:1997444/1‑150 (MQ=255)
                                                                                                                                     cATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGt                    >  1:1197523/1‑150 (MQ=255)
                                                                                                                                      aTTTCAAATGTGGTGCAGTACCTGTAAGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGTa                   >  2:325698/1‑150 (MQ=255)
                                                                                                                                           aaaTGTGGTGCAGTACCTGTAGGAGC‑GGGTTTAGCCGCGAGAGGGCCGGCGCAGTTTGTCGAGATGCATCATTTTTTCGACGGGGTTTATGACTCTGATTCTAGGTATCGGCCCCGGCTCGCGCATCACCGGCTATGGCGTGGTACGCCa              >  2:171391/1‑150 (MQ=255)
                                                                                                                                           aaaTGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGTACGCCa              >  2:2550106/1‑150 (MQ=255)
                                                                                                                                             aTGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGTACGCCAGa            <  1:1577085/150‑1 (MQ=255)
                                                                                                                                                tggtgCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGTACGCCAGACCg         >  1:1694382/1‑150 (MQ=255)
                                                                                                                                                  gtgCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCCGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGTACGCCAGgccgcc       >  2:2019929/1‑150 (MQ=255)
                                                                                                                                                  gtgCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGTACGCCAGACCGcc       >  2:2019412/1‑150 (MQ=255)
                                                                                                                                                    gCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGTACGCCAGACCGCCCg     >  2:1760898/1‑150 (MQ=255)
                                                                                                                                                       gTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTaga                                                                                                      <  2:3483886/50‑1 (MQ=255)
                                                                                                                                                       gTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTaga                                                                                                      >  1:3483886/1‑50 (MQ=255)
                                                                                                                                                       gTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGTACGCCAGACCGCCCGTgg  >  2:4469153/1‑150 (MQ=255)
                                                                                                                                                       |                                                                                                                                                      
GCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAA‑CCCGCTCCTACAGGTATTACG‑CC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGC‑GGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGTGGTACGCCAGACCGCCCGTGG  >  NC_002947/1392798‑1393095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑