Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,416,874 T→A 5.4% L39Q (CTG→CAG)  PP_1239 → metallo‑beta‑lactamase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,416,8740TA 5.4% 563.5 / 15.8 168L39Q (CTG→CAG) PP_1239metallo‑beta‑lactamase family protein
Reads supporting (aligned to +/- strand):  ref base T (91/68);  new base A (4/5);  total (95/73)
Fisher's exact test for biased strand distribution p-value = 5.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.43e-01

Only 100 of 168 total aligned reads displayed.

TGCTGAGCGCTATTCAGGACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAG  >  NC_002947/1416727‑1417022
                                                                                                                                                   |                                                                                                                                                    
tGCTGAGCGCTATTCAGGACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTgg                                                                                                                                                    <  1:3263977/150‑1 (MQ=255)
 gCTGAGCGCTATTCAGGACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGgc                                                                                                                                                   <  1:1426669/150‑1 (MQ=255)
        gcTATTCAGGACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCAGCGCCAGGCGCCGCTc                                                                                                                                            <  1:1634927/150‑1 (MQ=255)
         cTATTCAGGACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCAGCGCCAGGCGCCGCTCg                                                                                                                                           <  1:4045271/150‑1 (MQ=255)
         cTATTCAGGACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCAGCGCCAGGCGCCGCTCg                                                                                                                                           <  1:1160178/150‑1 (MQ=255)
          tATTCAGGACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCgg                                                                                                                                          <  2:1073830/150‑1 (MQ=255)
          tATTCAGGACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCgg                                                                                                                                          >  1:4473949/1‑150 (MQ=255)
          tATTCAGGACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCgg                                                                                                                                          <  2:1227592/150‑1 (MQ=255)
          tATTCAGGACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCgg                                                                                                                                          >  1:2916619/1‑150 (MQ=255)
           aTTCAGGACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCggg                                                                                                                                         <  1:365734/150‑1 (MQ=255)
           aTTCAGGACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCAGCGCCAGGCGCCGCTCggt                                                                                                                                         <  2:468906/150‑2 (MQ=255)
           aTTCAGGACCACCTGGGCTAAGTGCGCTTCGCGGTACCCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCggg                                                                                                                                         <  1:2518897/150‑1 (MQ=255)
            ttCAGGACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATCGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCgggg                                                                                                                                        >  1:3866031/1‑150 (MQ=255)
               aGGACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGttt                                                                                                                                     >  1:1150058/1‑150 (MQ=255)
               aGGACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGttt                                                                                                                                     >  1:1674366/1‑150 (MQ=255)
               aGGACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGttt                                                                                                                                     >  2:1990195/1‑150 (MQ=255)
                ggACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCATTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTc                                                                                                                                    <  2:349104/150‑1 (MQ=255)
                ggACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTc                                                                                                                                    >  1:2059342/1‑150 (MQ=255)
                 gACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCt                                                                                                                                   >  2:3410416/1‑150 (MQ=255)
                      ccTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGcagc                                                                                                                              >  2:3170192/1‑150 (MQ=255)
                        tGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGcagctc                                                                                                                            <  1:2138623/150‑1 (MQ=255)
                         gggCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGcagctca                                                                                                                           <  2:1546849/150‑1 (MQ=255)
                         gggCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGcagctca                                                                                                                           >  2:2270952/1‑150 (MQ=255)
                         gggCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGcagctca                                                                                                                           >  2:666415/1‑150 (MQ=255)
                            cTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGcagctcagct                                                                                                                        >  1:2434644/1‑150 (MQ=255)
                             tAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCagctcagctc                                                                                                                       >  2:3556213/1‑150 (MQ=255)
                              aaGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAgctcagctca                                                                                                                      >  2:1697311/1‑150 (MQ=255)
                               aGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGctcagctcag                                                                                                                     >  1:3043964/1‑150 (MQ=255)
                                  gcgcTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTcagtgc                                                                                                                  >  1:996077/1‑150 (MQ=255)
                                   cgcTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTcagtgca                                                                                                                 <  1:882206/150‑1 (MQ=255)
                                     cTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTcagtgcagt                                                                                                               <  1:3559034/150‑1 (MQ=255)
                                      ttCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTcagtgcagtg                                                                                                              >  2:3226283/1‑150 (MQ=255)
                                      ttCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTcagtgcagtg                                                                                                              >  2:2542887/1‑150 (MQ=255)
                                          cgGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTgg                                                                                                          >  2:125079/1‑150 (MQ=255)
                                           ggTACTCGGAAGCGGCAGCCAAGGGAACGGCACGGTGATCGCCAGTGTTGACACGTTCATCCTGGTCGCTTGCGGCTGTTCCCTGCGTGAATCCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGt                                                                                                         <  2:149385/150‑1 (MQ=255)
                                           ggTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGt                                                                                                         <  2:3066470/150‑1 (MQ=255)
                                            gTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGAGGTGACACGTACATCCTGGTCGATTGCGCCTTGTCCCTGCGTGACACCGAGCGGCGCCTGGCGCTGCTCGGGGTGTCTGCAGCTCAGCGCAGTGCAGAGCTGGTc                                                                                                        >  2:3837209/1‑150 (MQ=255)
                                                tCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCAccc                                                                                                    >  1:1342285/1‑150 (MQ=255)
                                                 cGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCa                                                                                                   <  1:2688787/150‑1 (MQ=255)
                                                   gAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACg                                                                                                 >  2:3109621/1‑150 (MQ=255)
                                                   gAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACg                                                                                                 >  2:738100/1‑150 (MQ=255)
                                                    aaGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGa                                                                                                <  1:2291671/150‑1 (MQ=255)
                                                    aaGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGa                                                                                                <  2:4472936/150‑1 (MQ=255)
                                                        ggCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACAt                                                                                            >  2:4453741/1‑150 (MQ=255)
                                                        ggCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACAt                                                                                            >  2:906123/1‑150 (MQ=255)
                                                         gCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATg                                                                                           >  2:1043132/1‑150 (MQ=255)
                                                            gCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCg                                                                                        >  1:2854934/1‑150 (MQ=255)
                                                             ccAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGa                                                                                       >  1:4365349/1‑150 (MQ=255)
                                                              cAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGAc                                                                                      >  2:2113871/1‑150 (MQ=255)
                                                               aaGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGAcc                                                                                     >  2:2923053/1‑150 (MQ=255)
                                                                   gAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGt                                                                                 <  2:3468941/150‑1 (MQ=255)
                                                                        gCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCTCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATg                                                                            <  2:1731650/150‑1 (MQ=255)
                                                                        gCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCAGGTGCATg                                                                            >  1:2833884/1‑150 (MQ=255)
                                                                          aCGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATggg                                                                          <  1:1398394/150‑1 (MQ=255)
                                                                          aCGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATggg                                                                          >  2:2144954/1‑150 (MQ=255)
                                                                          aCGCTGATCGCCAGTGGAGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATggg                                                                          >  2:2353327/1‑150 (MQ=255)
                                                                            gCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGt                                                                        >  2:2731641/1‑150 (MQ=255)
                                                                            gCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGt                                                                        >  1:942294/1‑150 (MQ=255)
                                                                                  cGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCtt                                                                  <  2:1155670/150‑1 (MQ=255)
                                                                                   gCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTg                                                                 >  1:2593431/1‑150 (MQ=255)
                                                                                   gCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTg                                                                 <  2:3275517/150‑1 (MQ=255)
                                                                                   gCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTg                                                                 >  1:2322688/1‑150 (MQ=255)
                                                                                   gCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTg                                                                 >  2:4301438/1‑150 (MQ=255)
                                                                                     cAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGTCGACCACGTGCATGGGGTCGGCTTGCt                                                               >  2:2420430/1‑150 (MQ=255)
                                                                                     cAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCt                                                               <  2:2618540/150‑1 (MQ=255)
                                                                                      aGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCCCATGCACGTGGTCGGCATgttc                                                              <  2:372308/150‑4 (MQ=255)
                                                                                       gtggtgACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGt                                                             <  1:4107833/150‑1 (MQ=255)
                                                                                           tgACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACg                                                         >  2:4106513/1‑150 (MQ=255)
                                                                                           tgACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGACGACCACGTGCATGGGGTCGGCTTGCTGTCACg                                                         >  1:812763/1‑150 (MQ=255)
                                                                                             acacGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGgc                                                       >  1:4415465/1‑150 (MQ=255)
                                                                                             acacGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGgc                                                       <  1:4518053/150‑1 (MQ=255)
                                                                                               acGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGgcgc                                                     >  1:3013124/1‑150 (MQ=255)
                                                                                                 gTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTa                                                   >  1:1045870/1‑150 (MQ=255)
                                                                                                 gTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTa                                                   >  1:3121050/1‑150 (MQ=255)
                                                                                                 gTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGCCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTa                                                   >  2:2394857/1‑150 (MQ=255)
                                                                                                    cATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACaa                                                <  1:869016/150‑1 (MQ=255)
                                                                                                         tGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTAc                                           <  2:2656674/150‑1 (MQ=255)
                                                                                                         tGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTAc                                           >  2:3931110/1‑150 (MQ=255)
                                                                                                         tGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTAc                                           >  1:311838/1‑150 (MQ=255)
                                                                                                         tGGTCGATTGCGGCTTTTCCCTCCGTGAAACCGAGCGGAGCCTGGAGCTGCTCGGGGTTTATGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATTCCGACCACGTGCAGGGGGTCGGCTTGATCTCACGGCGCTACAATGTaa                                           >  1:3263425/1‑149 (MQ=255)
                                                                                                                ttGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGACTa                                    >  2:2962356/1‑150 (MQ=255)
                                                                                                                  gCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTAcc                                  >  2:2681732/1‑150 (MQ=255)
                                                                                                                   cGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCt                                 <  2:2562754/150‑1 (MQ=255)
                                                                                                                    ggCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTc                                >  2:2490356/1‑150 (MQ=255)
                                                                                                                          tCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCaa                          >  1:362897/1‑150 (MQ=255)
                                                                                                                          tCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCaa                          <  1:1709789/150‑1 (MQ=255)
                                                                                                                          tCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCaa                          <  2:4316593/150‑1 (MQ=255)
                                                                                                                            ccTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAgg                        >  1:4149225/1‑150 (MQ=255)
                                                                                                                            ccTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAgg                        >  1:107767/1‑150 (MQ=255)
                                                                                                                            ccTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAgg                        >  1:1879564/1‑150 (MQ=255)
                                                                                                                             cTGCGTGAAACCGAGCGGCGCCTGGCGATGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCAACCAAGAACATGCCGACCACGTGCATGGTGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAggg                       >  1:1015281/1‑150 (MQ=255)
                                                                                                                                  tGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACttt                  >  2:3220007/1‑150 (MQ=255)
                                                                                                                                      cccGAGCAGCGCCAGGCGCCGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTgcgc              >  1:3343174/2‑150 (MQ=255)
                                                                                                                                       ccGAGCAGCGCCAGGCGCCGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGcgcg             >  1:2003061/1‑150 (MQ=255)
                                                                                                                                       ccGAGCAGCGCCAGGCGCCGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGcgcg             >  1:523662/1‑150 (MQ=255)
                                                                                                                                               cgcCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATgcgc     >  2:545182/1‑150 (MQ=255)
                                                                                                                                                gcCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCa    >  1:2765614/1‑150 (MQ=255)
                                                                                                                                                 ccTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCaa   <  2:1342285/150‑1 (MQ=255)
                                                                                                                                                 ccTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCaa   >  1:2255717/1‑150 (MQ=255)
                                                                                                                                                 ccTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCaa   <  2:1379601/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TGCTGAGCGCTATTCAGGACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAG  >  NC_002947/1416727‑1417022

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑