BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,831,819	G→A	6.2%	R219Q (CGG→CAG)	yfiP →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,831,819	0	G	A	6.2%	661.0 / 22.1	208	R219Q (CGG→CAG)	yfiP	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (82/112); new base A (3/10); total (86/122)
Fisher's exact test for biased strand distribution p-value = 2.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 221 total aligned reads displayed.

CCGCCTGCGGCGCTCCAAGCGCGATGATCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCAGGGCGATTGGCTTGATCTCCCTTG  >  NC_002947/1831670‑1831966

ccGCCTGCGGCGCTCCAAGCGCGATGATCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAgcggcg                                                                                                                                                     <  1:662288/150‑1 (MQ=255)

 cGCCTGCGGCGCTCCAAGCGCGATGATCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGcggcgg                                                                                                                                                    <  1:1079376/150‑1 (MQ=255)

 cGCCTGCGGCGCTCCAAGCGCGATGATCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGcggcgg                                                                                                                                                    >  2:467620/1‑150 (MQ=255)

 cGCCTGCGGCGCTCCAAGCGCGATGATCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGcggcgg                                                                                                                                                    <  1:3292127/150‑1 (MQ=255)

 cGCCTGCGGCGCTCCAAGCGCGATGATCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGcggcgg                                                                                                                                                    <  1:1817932/150‑1 (MQ=255)

  gCCTGCGGCGCTCCAAGCGCGATGATCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCggcggc                                                                                                                                                   >  2:2029287/1‑150 (MQ=255)

     tGCGGCGCTCCAAGCGCGATGATCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGc                                                                                                                                                <  2:3181445/150‑1 (MQ=255)

           gcTCCAAGCGCGATGATCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGTCAAGCGGCAGCCGCCGCTTg                                                                                                                                          <  2:1197900/150‑1 (MQ=255)

           gcTCCAAGCGCGATGATCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCAGCCGCCGCTTg                                                                                                                                          <  1:2696432/150‑1 (MQ=255)

            cTCCAAGCGCGATGATCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCAGCCGCCGCTTgc                                                                                                                                         <  2:1960266/150‑2 (MQ=255)

            cTCCAAGCGCGATGATCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCACAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGa                                                                                                                                         <  2:2314639/150‑1 (MQ=255)

             tCCAAGCGCGATGATCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCAGCCGCCGCTTgcc                                                                                                                                        <  2:1169609/150‑3 (MQ=255)

             tCCAAGCGCGATGATCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCAGCCGCCGCTTgcc                                                                                                                                        <  2:4229549/150‑3 (MQ=255)

               cAAGCGCGATGATCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGa                                                                                                                                      >  2:2754443/1‑150 (MQ=255)

                aaGCGCGATGATCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAg                                                                                                                                     <  2:3570804/150‑1 (MQ=255)

                     cgATGATCATTTCTGGACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCTAGCGGCGGCTGCCGCTTGACGAGCAGga                                                                                                                                <  2:3962792/150‑1 (MQ=255)

                      gatgatCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGgac                                                                                                                               <  1:1518546/150‑1 (MQ=255)

                       atgatCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGgacg                                                                                                                              >  2:3048929/1‑150 (MQ=255)

                       atgatCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGgacg                                                                                                                              >  1:1814164/1‑150 (MQ=255)

                         gatCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGgacgac                                                                                                                            >  1:3878709/1‑150 (MQ=255)

                          atCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGaca                                                                                                                           >  1:4148928/1‑150 (MQ=255)

                             aTTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGAcaccc                                                                                                                        <  1:1834515/150‑1 (MQ=255)

                             aTTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGAcaccc                                                                                                                        >  2:4057587/1‑150 (MQ=255)

                              tttCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGAcaccca                                                                                                                       >  2:3854445/1‑150 (MQ=255)

                               ttCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGAcacccac                                                                                                                      <  1:637308/150‑1 (MQ=255)

                                 cTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACccaaca                                                                                                                    <  2:2632096/150‑1 (MQ=255)

                                  tGTACGGCAGACGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAg                                                                                                                   <  2:6303/150‑1 (MQ=255)

                                  tGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGCCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAg                                                                                                                   <  1:20411/150‑1 (MQ=255)

                                      cGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGtt                                                                                                               <  2:2199988/150‑1 (MQ=255)

                                       ggCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGttt                                                                                                              <  1:2152040/150‑1 (MQ=255)

                                       ggCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGttt                                                                                                              <  1:2830323/150‑1 (MQ=255)

                                       ggCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGttt                                                                                                              <  1:2369109/150‑1 (MQ=255)

                                        gCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTg                                                                                                             <  1:4195982/150‑1 (MQ=255)

                                         cAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGc                                                                                                            >  2:1188250/1‑150 (MQ=255)

                                                   ggcgATGTGCCTGGACCTTGCTGGCGATGTCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCt                                                                                                  >  2:4445172/1‑150 (MQ=255)

                                                   ggcgATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCt                                                                                                  <  1:3178677/150‑1 (MQ=255)

                                                   ggcgATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCt                                                                                                  <  1:1256076/150‑1 (MQ=255)

                                                   ggcgATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCt                                                                                                  >  1:2277148/1‑150 (MQ=255)

                                                    gcgATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCta                                                                                                 >  2:1974751/1‑150 (MQ=255)

                                                    gcgATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCta                                                                                                 <  2:2523847/150‑1 (MQ=255)

                                                    gcgATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCta                                                                                                 >  1:1560421/1‑150 (MQ=255)

                                                      gATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCtata                                                                                               <  1:3543474/150‑1 (MQ=255)

                                                      gATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCtata                                                                                               <  2:3151848/150‑1 (MQ=255)

                                                      gATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCtata                                                                                               <  2:3029455/150‑1 (MQ=255)

                                                           gCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCTGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCa                                                                                          >  1:211952/1‑150 (MQ=255)

                                                              tGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAgg                                                                                       >  2:1391447/1‑150 (MQ=255)

                                                              tGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAgg                                                                                       <  2:2975608/150‑1 (MQ=255)

                                                                gACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGTGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAgggg                                                                                     <  2:814931/150‑1 (MQ=255)

                                                                gACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAgggg                                                                                     <  2:3838487/150‑1 (MQ=255)

                                                                         ggCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAAcccc                                                                            <  2:3814124/150‑1 (MQ=255)

                                                                           cGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTg                                                                          <  2:578216/150‑1 (MQ=255)

                                                                              tGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCtt                                                                       <  2:2945025/150‑1 (MQ=255)

                                                                              tGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCtt                                                                       <  2:1325451/150‑1 (MQ=255)

                                                                               ggCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCttt                                                                      <  2:2825305/150‑1 (MQ=255)

                                                                                 ccAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTgg                                                                    >  2:1349965/1‑150 (MQ=255)

                                                                                  cAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGt                                                                   <  2:510296/150‑1 (MQ=255)

                                                                                  cAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGt                                                                   >  2:1388773/1‑150 (MQ=255)

                                                                                  cAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGt                                                                   >  2:1877718/1‑150 (MQ=255)

                                                                                    ggCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCATTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTc                                                                 <  2:762172/150‑1 (MQ=255)

                                                                                    ggCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGAATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCATTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTc                                                                 <  1:1053526/150‑1 (MQ=255)

                                                                                      cTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCAt                                                               >  1:3887933/1‑150 (MQ=255)

                                                                                            aGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGAc                                                         >  1:2965335/1‑150 (MQ=255)

                                                                                            aGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGAc                                                         >  1:3150949/1‑150 (MQ=255)

                                                                                               cgcTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGa                                                      <  1:762053/150‑1 (MQ=255)

                                                                                                gcTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAg                                                     >  2:1490996/1‑150 (MQ=255)

                                                                                                   ggATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTg                                                  <  2:1684583/150‑1 (MQ=255)

                                                                                                   ggATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTg                                                  <  1:1187441/150‑1 (MQ=255)

                                                                                                    gATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTgg                                                 >  1:2429233/1‑150 (MQ=255)

                                                                                                      tGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCa                                               <  1:4303512/150‑1 (MQ=255)

                                                                                                             cTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAggg                                        <  1:4356861/150‑1 (MQ=255)

                                                                                                              tGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGc                                                                                                                                    <  1:931145/57‑1 (MQ=255)

                                                                                                                gATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTgg                                     >  2:4157703/1‑150 (MQ=255)

                                                                                                                 aTGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTggg                                    <  1:2029287/150‑1 (MQ=255)

                                                                                                                  tgtgTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGc                                   <  2:248280/150‑1 (MQ=255)

                                                                                                                    tgtTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCga                                 <  1:2217950/150‑1 (MQ=255)

                                                                                                                    tgtTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCga                                 <  1:1164421/150‑1 (MQ=255)

                                                                                                                      ttCAGCCTGCATTACCTGAGCGGCAAGCTGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCgaga                               <  1:367164/150‑1 (MQ=255)

                                                                                                                      ttCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCgaga                               <  2:89140/150‑1 (MQ=255)

                                                                                                                      ttCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCgaga                               >  2:1930362/1‑150 (MQ=255)

                                                                                                                      ttCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCgaga                               >  2:1986975/1‑150 (MQ=255)

                                                                                                                      ttCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCgaga                               >  1:3574490/1‑150 (MQ=255)

                                                                                                                      ttCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTGTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCgaga                               >  2:3762703/1‑150 (MQ=255)

                                                                                                                           ccTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCa                          >  1:792697/1‑150 (MQ=255)

                                                                                                                           ccTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCa                          <  1:2716574/150‑1 (MQ=255)

                                                                                                                                aTTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCAGGGCg                     >  1:1370541/1‑150 (MQ=255)

                                                                                                                                 ttACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCAGGGCGa                    <  1:972538/150‑1 (MQ=255)

                                                                                                                                  tACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCAGGGCGAt                   >  1:1385068/1‑150 (MQ=255)

                                                                                                                                   aCCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCAGGGCGAtt                  <  1:3446497/150‑1 (MQ=255)

                                                                                                                                   aCCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCAGGGCGAtt                  <  2:1271996/150‑1 (MQ=255)

                                                                                                                                   aCCGGAGCGGCAAGCGGCGGCTGCCGCGTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTAGAGTCCCCGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCAGGGCGAtt                  <  2:4125810/150‑1 (MQ=255)

                                                                                                                                      tGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCAGGGCGATTGGc               >  1:432028/1‑150 (MQ=255)

                                                                                                                                       aaGCGGCAGCCGCCGCTTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTggg                                    <  2:781703/127‑1 (MQ=255)

                                                                                                                                       aaGCGGCAGCCGCCGCTTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTggg                                    >  1:781703/2‑128 (MQ=255)

                                                                                                                                         tcgtcAAGCGGCAGCCGCCGCTTGCCGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCAGGGCGATTGGCTTg            >  1:2560186/5‑150 (MQ=255)

                                                                                                                                           ggCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCAGGGCGATTGGCTTGAt          >  1:425390/1‑150 (MQ=255)

                                                                                                                                            gCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCAGGGCGATTGGCTTGAtc         >  1:4476842/1‑150 (MQ=255)

                                                                                                                                              aaGCGGCAGCCGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCAGGGCGATTGGCTTGAtctc       >  2:1436062/1‑150 (MQ=255)

                                                                                                                                               aGCGGCAGCCGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCAGGGCGATTGGCTTGATCTcc      >  2:2402986/1‑150 (MQ=255)

                                                                                                                                                gcggcgGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCAGGGCGATTGGCTTGATCTccc     >  1:148258/1‑150 (MQ=255)

                                                                                                                                                   gcggcTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCAGGGCGATTGGCTTGATCTCCCTTg  >  1:2911152/1‑150 (MQ=255)

CCGCCTGCGGCGCTCCAAGCGCGATGATCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCAGGGCGATTGGCTTGATCTCCCTTG  >  NC_002947/1831670‑1831966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`