BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,863,282	T→A	5.2%	L309Q (CTG→CAG)	PP_1666 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,863,282	0	T	A	5.2%	591.9 / 14.3	172	L309Q (CTG→CAG)	PP_1666	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (86/76); new base A (4/5); total (90/82)
Fisher's exact test for biased strand distribution p-value = 7.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

Only 100 of 185 total aligned reads displayed.

GGTGCAAGTGCAGGGCATGAACCTGCAGCGCTATGCCGAGCTGGCCCGCGTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAGGAGCG  >  NC_002947/1863133‑1863430

ggTGCAAGTGCAGGGCATGAACCTGCAGCGCTATGCCGAGCTGGCCCGCGTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTtggcct                                                                                                                                                      <  1:105792/150‑1 (MQ=255)

 gTGCAAGTGCAGGGCATGAACCTGCAGCGCTATGCCGAGCTGGCCCGCGTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTtggcctg                                                                                                                                                     >  2:2312306/1‑150 (MQ=255)

 gTGCAAGTGCAGGGCATGAACCTGCAGCGCTATGCCGAGCTGGCCCGCGTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTtggcctg                                                                                                                                                     >  2:57227/1‑150 (MQ=255)

  tGCAAGTGCAGGGCATGAACCTGCAGCGCTATGCCGAGCTGGCCCGCGTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTtggcctgg                                                                                                                                                    <  1:652473/150‑1 (MQ=255)

    cAAGTGCAGGGCATGAACCTGCAGCGCTATGCCGAGCTGGCCCGCGTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTtggcctggcc                                                                                                                                                  <  1:541820/150‑1 (MQ=255)

       gTGCAGGGCATGAACCTGCAGCGCTATGCCGAGCTGGCCCGCGTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAg                                                                                                                                               <  2:3865306/150‑1 (MQ=255)

       gTGCAGGGCATGAACCTGCAGCGCTATGCCGAGCTGGCCCGCGTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAg                                                                                                                                               >  2:2387154/1‑150 (MQ=255)

        tGCAGGGCATGAACCTGCAGCGCTATGCCGAGCTGGCCCGCGTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGc                                                                                                                                              >  1:3414105/1‑150 (MQ=255)

        tGCAGGGCATGAACCTGCAGCGCTATGCCGAGCTGGCCCGCGTGCGTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGc                                                                                                                                              >  1:630057/1‑150 (MQ=255)

          cAGGGCATGAACCTGCAGCGCTATGCCGAGCTGGCCCGCGTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCAGGCCAAGCTg                                                                                                                                            <  1:3009064/150‑1 (MQ=255)

          cAGGGCATGAACCTGCAGCGCTATGCCGAGCTGGCCCGCGTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCAGGCCAAGCTg                                                                                                                                            <  1:2163769/150‑1 (MQ=255)

           aGGGCATGAACCTGCAGCGCTATGCCGAGCTGGCCCGCGTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGc                                                                                                                                           <  2:3830634/150‑1 (MQ=255)

           aGGGCATGAACCTGCAGCGCTATGCCGAGCTGGCCCGCGTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCAGGCCAAGCTgg                                                                                                                                           <  2:3561156/150‑2 (MQ=255)

            gggCATGAACCTGCAGCGCTATGCCGAGCTGGCCCGCGTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCa                                                                                                                                          <  1:4330404/150‑1 (MQ=255)

             ggCATGAACCTGCAGCGCTATGCCGAGCTGGCCCGCGTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAg                                                                                                                                         >  1:1056424/1‑150 (MQ=255)

             ggCATGAACCTGCAGCGCTATGCCGAGCTGGCCCGCGTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAg                                                                                                                                         >  2:2548217/1‑150 (MQ=255)

              gCATGAACCTGCAGCGCTATGCCGAGCTGGCCCGCGTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAgg                                                                                                                                        >  1:120714/1‑150 (MQ=255)

               cATGAACCTGCAGCGCTATGCCGAGCTGGCCCGCGTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGa                                                                                                                                       <  2:2648720/150‑1 (MQ=255)

                aTGAACCTGCAGCGCTATGCCGAGCTGGCCCGCGTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGaa                                                                                                                                      >  1:2288839/1‑150 (MQ=255)

                 tGAACCTGCAGCGCTATGCCGAGCTGGCCCGCGTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAg                                                                                                                                     <  1:1878276/150‑1 (MQ=255)

                   aaCCTGCAGCGCTATGCCGAGCTGGCCCGCGTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTg                                                                                                                                   >  1:1266142/1‑150 (MQ=255)

                      cTGCAGCGCTATGCCGAGCTGGCCCGCGTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCg                                                                                                                                <  2:3689551/150‑1 (MQ=255)

                       tGCAGCGCTATGCCGAGCTGGCCCGCGTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCgg                                                                                                                               >  1:2450903/1‑150 (MQ=255)

                       tGCAGCGCTATGCCGAGCTGGCCCGCGTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCgg                                                                                                                               >  2:3246127/1‑150 (MQ=255)

                            cgcTATGCCGAGCTGGCCCGCGTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAg                                                                                                                          >  1:4206409/1‑150 (MQ=255)

                             gcTATGCCGAGCTGGCCCGCGTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGc                                                                                                                         <  2:607259/150‑1 (MQ=255)

                              cTATGCCGAGCTGGCCCGCGTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCa                                                                                                                        <  1:3520374/150‑1 (MQ=255)

                              cTATGCCGAGCTGGCCCGCGTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCa                                                                                                                        <  2:3986063/150‑1 (MQ=255)

                               tATGCCGAGCTGGCCCGCGTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAg                                                                                                                       >  1:716227/1‑150 (MQ=255)

                               tATGCCGAGCTGGCCCGCGTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAg                                                                                                                       <  1:268054/150‑1 (MQ=255)

                                    cGAGCTGGCCCGCGTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCAcc                                                                                                                  >  2:2849466/1‑150 (MQ=255)

                                        cTGGCCCGCGTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACcggcg                                                                                                              >  1:2914275/1‑150 (MQ=255)

                                         tGGCCCGCGTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACcggcgg                                                                                                             <  2:990970/150‑1 (MQ=255)

                                          ggCCCGCGTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGt                                                                                                            <  1:57227/150‑1 (MQ=255)

                                             ccGCGTGCTTGAGCCCTATGGTCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGcc                                                                                                         <  2:4342762/150‑1 (MQ=255)

                                             ccGCGTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGcc                                                                                                         >  2:2591165/1‑150 (MQ=255)

                                             ccGCGTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGcc                                                                                                         >  1:3506390/1‑150 (MQ=255)

                                              cgcgTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCg                                                                                                        <  1:367177/150‑1 (MQ=255)

                                              cgcgTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCg                                                                                                        >  1:3079059/1‑150 (MQ=255)

                                                 gTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGCCGGTGCCTGct                                                                                                     <  1:706595/150‑2 (MQ=255)

                                                     ttGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGc                                                                                                 >  2:4309055/1‑150 (MQ=255)

                                                       gAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCa                                                                                               <  2:2088310/150‑1 (MQ=255)

                                                          cccTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTa                                                                                            >  2:1326264/1‑150 (MQ=255)

                                                          cccTATGGCACACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTAGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTa                                                                                            >  2:1734232/1‑150 (MQ=255)

                                                            cTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTCCGGGGTTACGGGCAATCGTGACCAGCGACGGGCCCAGCTTGGCCTGGCCTAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATACCAGTAcc                                                                                          <  2:2420068/150‑1 (MQ=255)

                                                             tATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCg                                                                                         >  1:3040552/1‑150 (MQ=255)

                                                                  cccACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCGGCAGt                                                                                    >  2:1773816/1‑150 (MQ=255)

                                                                    cACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCGGCAGTgg                                                                                  >  1:2585075/1‑150 (MQ=255)

                                                                    cACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCGGCAGTgg                                                                                  <  2:823020/150‑1 (MQ=255)

                                                                     tattgtGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTCCCGGCAGTGGa                                                                                 >  2:3159981/6‑150 (MQ=255)

                                                                             aGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCACTCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCGGCAGTGGACGCCGCAg                                                                         >  2:956873/1‑150 (MQ=255)

                                                                               gcggcTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCGGCAGTGGACGCCGCAGcc                                                                       <  2:4416340/150‑1 (MQ=255)

                                                                                  gcTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCGGCAGTGGACGCCGCAGCcgcg                                                                    <  2:2288839/150‑1 (MQ=255)

                                                                                    tGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCa                                                                  >  2:1452170/1‑150 (MQ=255)

                                                                                    tGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCa                                                                  >  1:2616863/1‑150 (MQ=255)

                                                                                              aCCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGTAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCt                                                        <  1:2890240/150‑1 (MQ=255)

                                                                                               cccTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTg                                                       <  2:3386645/150‑1 (MQ=255)

                                                                                                ccTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTgc                                                      >  1:4170772/1‑150 (MQ=255)

                                                                                                  tGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTgcgc                                                    >  1:2332169/1‑150 (MQ=255)

                                                                                                    aCCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGcag                                                  >  1:2661841/1‑150 (MQ=255)

                                                                                                     ccTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGcagc                                                 <  1:1958898/150‑1 (MQ=255)

                                                                                                     ccTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGcagc                                                 <  1:3265927/150‑1 (MQ=255)

                                                                                                      cTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGcagcc                                                <  2:219470/150‑1 (MQ=255)

                                                                                                       tACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGcagcca                                               >  2:1393348/1‑150 (MQ=255)

                                                                                                          ggggTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCa                                            >  2:622572/1‑150 (MQ=255)

                                                                                                            ggTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCAcc                                          >  2:4056420/1‑150 (MQ=255)

                                                                                                            ggTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCAcc                                          >  1:1138657/1‑150 (MQ=255)

                                                                                                             gTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCAccc                                         <  1:2150044/150‑1 (MQ=255)

                                                                                                               tACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCaa                                       <  1:1475009/150‑1 (MQ=255)

                                                                                                                aCGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAg                                      >  1:668537/1‑150 (MQ=255)

                                                                                                                aCGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAg                                      >  1:2203219/1‑150 (MQ=255)

                                                                                                                      aaTCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTc                                >  2:424861/1‑150 (MQ=255)

                                                                                                                      aaTCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTc                                <  1:4363202/150‑1 (MQ=255)

                                                                                                                      aaTCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTc                                <  1:4492815/150‑1 (MQ=255)

                                                                                                                        tCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGTGGTGCCGGCCATGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGa                              <  2:3414233/150‑1 (MQ=255)

                                                                                                                          gTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACg                            >  2:1794060/1‑150 (MQ=255)

                                                                                                                              aCAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCtt                        >  2:4432647/1‑150 (MQ=255)

                                                                                                                              aCAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCtt                        >  2:2683585/1‑150 (MQ=255)

                                                                                                                               cAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTg                       <  2:1218473/150‑1 (MQ=255)

                                                                                                                                aGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGc                      >  2:2015399/1‑150 (MQ=255)

                                                                                                                                      gTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTc                <  1:1993632/150‑1 (MQ=255)

                                                                                                                                      gTGCCCAGCTGGGCCGGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCGGCAGTGGACGCCGCCGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTc                <  2:1112534/150‑1 (MQ=255)

                                                                                                                                       tGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCg               <  1:4089739/150‑1 (MQ=255)

                                                                                                                                        gCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGc              >  1:1834505/1‑150 (MQ=255)

                                                                                                                                         tgcAGCTTGGCCAGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCt             >  2:4273591/3‑150 (MQ=255)

                                                                                                                                         cccAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCt             <  1:2936935/150‑1 (MQ=255)

                                                                                                                                         cccAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTGTTCGCt             >  2:920917/1‑150 (MQ=255)

                                                                                                                                          ccAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCAcc                                          <  2:3819804/120‑1 (MQ=255)

                                                                                                                                          ccAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCAcc                                          >  1:3819804/1‑120 (MQ=255)

                                                                                                                                          ccAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTg            <  1:710791/150‑1 (MQ=255)

                                                                                                                                          ccAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTg            >  2:107103/1‑150 (MQ=255)

                                                                                                                                           cAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTgg           >  2:488874/1‑150 (MQ=255)

                                                                                                                                           cAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTACGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTgg           >  1:177380/1‑150 (MQ=255)

                                                                                                                                           cAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGACGGCCATTCCACTACAGCAAGTGGACGACGCAGACTCGCAGCCAGAGCATGCGCAGACAGCACCCAAGCCGTTCGACGGCTTGCTTTTTCGCTgg           >  2:4102509/1‑150 (MQ=255)

                                                                                                                                            aGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTGGt          <  2:2899157/150‑1 (MQ=255)

                                                                                                                                            aGCTTGGCCAGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTGGt          >  2:3180228/1‑150 (MQ=255)

                                                                                                                                             gCTTGGCCAGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTGGTa         >  1:3200079/1‑150 (MQ=255)

                                                                                                                                               ttGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAg       >  1:2156248/1‑150 (MQ=255)

                                                                                                                                               ttGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAg       <  2:1647859/150‑1 (MQ=255)

                                                                                                                                                   cctggccAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAGGAgc   >  1:901632/1‑150 (MQ=255)

GGTGCAAGTGCAGGGCATGAACCTGCAGCGCTATGCCGAGCTGGCCCGCGTGCTTGAGCCCTATGGCCCACGCTTGCAGGCGGCTGAAGGCACTACCCTGACCTACGGGGTTACGGGCAATCGTGAACAGCTACGTGCCCAGCTTGGCCTGGCCAAGCTGCAGGAAGTGCCGGTCGAGCAGGCACCGGCGGTGCCGGCCATGCCAGTACCGGCAGTGGACGCCGCAGCCGCGCAGCCCGCGCCTGCGCAGCCAGCACCCAAGCCGTTCGACGGCTTGCGTTTTCGCTGGTAAGGAGCG  >  NC_002947/1863133‑1863430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`