| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,243,279 | T→C | 16.1% | intergenic (+61/‑220) | gltX → / → PP_t25 | glutamate‑‑tRNA ligase/tRNA‑Ala |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,243,279 | 0 | T | C | 16.1% | 123.0 / 14.9 | 56 | intergenic (+61/‑220) | gltX/PP_t25 | glutamate‑‑tRNA ligase/tRNA‑Ala |
| Reads supporting (aligned to +/- strand): ref base T (13/31); new base C (6/2); total (22/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.98e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.07e-01 | |||||||||||
GCCTGCGCCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTG‑AAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGAACTTAATATGCGCC > NC_002947/2243133‑2243428 | gCCTGCGCCAGGCTCTGGACCTGCTGGGTGGGGTGTCTAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCATCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCCATATc < 2:2759618/150‑1 (MQ=255)gCCTGCGCCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATc < 1:610762/150‑1 (MQ=255)gCCTGCGCCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATc < 1:610566/150‑1 (MQ=255) ccTGCGCCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCg < 1:11299/150‑1 (MQ=255) gcCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGGGTGGCCGCGGATGCAATGGTTAATTAAATATAGCAtta > 2:2894020/1‑149 (MQ=255) gcCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTc < 1:2354251/150‑1 (MQ=255) gcCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTc < 2:2865741/150‑1 (MQ=255) gcCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTc < 2:3319331/150‑1 (MQ=255) ccAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAAATGTAAATTCAAAATACCAttag > 2:4478051/1‑148 (MQ=255) ctGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCGGCGAATGCGATATTGAATTTACCATTGCATTCGCGGGCAc < 1:809908/150‑1 (MQ=25) ggACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCGGCGAATGCGATATCGAATTTACCATTGCATTCGCGGGCACGc < 1:3164548/150‑1 (MQ=25) ctgGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGGGCCCCCGCAAGCAATGGGAAAATAAATATAGCAATAGCCGGCACGcccccgcc > 2:588072/1‑145 (MQ=255) tgtCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGGGGGGCCGCCCGTGCAATGGTTAAATAAAAAAAGCAGTAGCCGGCACCCCCGCGCCCCCACGccca > 1:3280078/1‑148 (MQ=255) gaaagaaaACAAAGAGTGGGAAATGCTGTTGGCTTCCATCGCGTGATTCAGCTTTGCACCTAACCTGTGGGAGCGGGCGTGCCCGCTATTGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGcc < 2:1329582/150‑1 (MQ=39) aaGCGTGGGAAAAGCTTTGGGGTTCCAGCGCCTTATACTGCTTTGCACCTACCCAGTGGGCGGGGGCGTGCCCTCGAATGCATTGGTAAATCCAATATCGCATTCGCGGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCg < 1:4310830/150‑1 (MQ=255) gAAATGCTGTTGGCTTCCATCGCCTTATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCTATTGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAggg < 2:480681/150‑1 (MQ=255) gAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCATTGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAggg < 2:3213441/150‑1 (MQ=255) gCTTCCATCGCCTTATTCCGCTTTGCACCTACCCCGGGGGTGGGGGGGTGCCGGCTATTGCATTGTTAAATCCAATCCCGCTGCCGCCGGCCCGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGAtt < 1:3822735/150‑1 (MQ=255) gCTTCCATCGCCTGATTCAGCTTTGCACCTAACCCGTGGGAGCGGGCGTGCCCGCGAATGCAATGTTAAATTCACTATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGAtt < 2:1140926/150‑1 (MQ=255) gCTTCCATCGCCGTATTCTGCTTTCCACCTACCCAGTGGGAGCGGGCGTGCCCGCTAATGCAATGTTAAATACAATATCGCTTGCGCCGCCCCGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGAtt < 1:2031543/150‑1 (MQ=255) tCCTTCGCCTGATTCAGCTTTGCACCAAACCTGTGGGAGCGGGGGTGCCCGGGAATGCAATGTTTAATACATTACCCCTTTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGtt < 1:952183/150‑1 (MQ=255) aGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAAAGGTAAAATCAAAATAGCAATCGCCGGCCCGCCCGCTCCCCCACGCCCACAAACCCCCAAAAAACCGGGGTAGGGCGGTAAGAGATTGTTAACTGTG‑CAAAAAtt > 2:4032985/1‑150 (MQ=255) aCCCGTGGGAGCGGGCGGGCCCGCGAATGCATTGGTAAATTCAATACCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTG‑AAAAAATTTTTGAATAttttt < 1:306543/150‑1 (MQ=255) aCCACTGCGAGCGGGAGTGCCCGCGAATGCAATGCTAAAAACAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGCGCGGTAAGTGATTGTTATCTGTG‑AAAAAAATTTTGAATAttttt < 2:2658707/150‑1 (MQ=255) gggAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTG‑AAAAAATTTTTGAATATTTTTGAAAAt < 1:4478051/150‑1 (MQ=255) gtgCGGGCGTGCCCGCGAATGGAATGGTAAATTCAATATCGCAGTCGCCGGCAGGCCCACTGCCTCAGTCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTG‑AAAAAATTTTTGAATATTTTTGAAAATTa < 2:35781/148‑1 (MQ=255) gAGCGGGGGTGCCCGCTAATGCATTGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTG‑AAAAAATTTTTGAATATTTTTGAAAATTa < 2:36045/150‑1 (MQ=255) gAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTG‑AAAAAATTTTTGAATATTTTTGAAAATTa < 2:3794917/150‑1 (MQ=255) gAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCACTCCCACAGTCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTG‑AAAAAATTTTTGAATATTTTTGAAAATTa < 2:35980/150‑1 (MQ=255) gAGCGGGCGTGCCCGCGAATGCAAGGGTAACTTCAATCTCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAATCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTG‑AAAAAATTTTTGAATATTTTTGAAAATTa < 2:3545366/150‑1 (MQ=255) cGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCCTTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTG‑AAAAAATTTTTGAATATTTTTGAAAATTAGtt < 2:1646724/150‑1 (MQ=255) gggCGTGCCGGCGAATGCGATATTGAATTTACCATTGCATTCGCGGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTG‑AAAAAATTTTTGAATATTTTTGAAAATTAGttt > 2:1684821/1‑150 (MQ=25) gggCGTGCCGGCGAATGCGATATTGAATTTACCATTGCATTCGCGGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTG‑AAAAAATTTTTGAATATTTTTGAAAATTAGttt > 1:1496016/1‑150 (MQ=25) gggCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTG‑AAAAAATTTTTGAATATTTTTGAAAATTAGttt > 2:3198621/1‑150 (MQ=255) gggCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTG‑AAAAAATTTTTGAATATTTTTGAAAATTAGttt < 2:432061/150‑1 (MQ=255) gCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTG‑AAAAAATTTTTGAATATTTTTGAAAATTAGTTTGa > 1:408811/1‑150 (MQ=255) gTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTG‑AAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACa > 1:3662264/1‑150 (MQ=255) gTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTG‑AAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACa > 1:3819381/1‑150 (MQ=255) tGCCGGCGAATGCGATATTGAATTTACCATTGCATTCGCGGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTG‑AAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAg > 1:3666189/1‑150 (MQ=25) tGCCGGCGAATGCGATATTGAATTTACCATTGCATTCGCGGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTG‑AAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAg > 1:2620767/1‑150 (MQ=25) tGCCGGCGAATGCGATATTGAATTTACCATTGCATTCGCGGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGAG‑AAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAg > 1:2620540/1‑150 (MQ=25) cggcgAATGCGATATTGAATTTACCATTGCATTCGCGGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTG‑AAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGccc > 2:1167863/3‑150 (MQ=25) ccGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTG‑AAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGccc > 2:2270928/1‑150 (MQ=255) ccGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTG‑AAAAAATGTTTGAATATTTTTGAAAATTAGTTTGACAGccc > 2:969729/1‑150 (MQ=255) gcgAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTG‑AAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTg < 2:2062392/150‑1 (MQ=255) cAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTG‑AAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCg > 1:3658698/1‑150 (MQ=255) cAATGGTAAATTCAATATCGCATACGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTG‑AAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCg < 2:3593577/150‑1 (MQ=255) aaTGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTG‑AAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGa > 1:4100594/1‑150 (MQ=255) aaTGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTG‑AAAAAATTTTTGAATATTTTTGAAAAGTAGTTTGACAGCCCTGCAATCCGa > 2:2622030/1‑150 (MQ=255) aTGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTG‑AAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGaa < 2:2071796/150‑1 (MQ=255) tgttAAATTCAAAATCGCATGCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTG‑AAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGAAc < 1:76997/147‑1 (MQ=255) ggttAAATTCACTACCGCCTCCGCCGCCCCCCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTG‑AAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGAAc < 1:1301481/147‑1 (MQ=255) aaaTTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTG‑AAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGAACTTaa < 2:2085460/150‑1 (MQ=255) ttCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGAACTTAata < 2:1456422/150‑1 (MQ=255) ttCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAAATAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTG‑AAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGATCTTAatat > 1:1780351/1‑150 (MQ=255) aaTATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTG‑AAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGAACTTAATATgcg > 2:3413857/1‑150 (MQ=255) tatCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTG‑AAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGAACTTAATATGCGcc > 2:2996396/1‑150 (MQ=255) | GCCTGCGCCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCCAACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTG‑AAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGAACTTAATATGCGCC > NC_002947/2243133‑2243428 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |