Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,445,467 T→A 6.2% F6L (TTT→TTA) ‡ PP_2140 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,445,4670TA 6.2% 600.3 / 16.7 174F6L (TTT→TTA) ‡PP_2140hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (78/84);  new base A (4/7);  total (82/92)
Fisher's exact test for biased strand distribution p-value = 5.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 179 total aligned reads displayed.

GATCCGCTACAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGCCAAGGCTGAACAAAGTCA  >  NC_002947/2445320‑2445616
                                                                                                                                                   |                                                                                                                                                     
gATCCGCTACAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGc                                                                                                                                                     >  1:4450915/1‑150 (MQ=255)
  tCCGCTACAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCgg                                                                                                                                                   <  2:2337697/150‑1 (MQ=255)
  tCCGCTACAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCgg                                                                                                                                                   <  2:101473/150‑1 (MQ=255)
     gCTACAGCCGCAAGACCCAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCGGGCAGGTGGAAGATAAGCGCTGATCCATGGGTGCTGTGATTTGGGGCCGCTTTGCGGccc                                                                                                                                                >  2:2265625/1‑150 (MQ=255)
     gCTACAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGccc                                                                                                                                                <  2:2254737/150‑1 (MQ=255)
     gCTACAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGccc                                                                                                                                                >  1:4258790/1‑150 (MQ=255)
     gCTACAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCAAAGCGGccc                                                                                                                                                <  2:4469668/150‑1 (MQ=255)
      cTACAGCCGCAAGACCAAGGAGCTGTTTGTGCTGTCCGAGGTCGTTGGCAAGCCGACCGGCTGTAAGGCATTCTACGAGGTAAATGCGTGGATGGTTGAAGATAAGCGCTGATCCTTTGTTGCTTTGTTTTGGGGCCGCAACGCGGcccc                                                                                                                                               <  1:2664766/150‑1 (MQ=255)
      cTACAGCCGCAAGACCAAGGAGCAGTATGTGCAGTGCGAGGTCGATGGCAAGCCGACCGTCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGTTCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGcccc                                                                                                                                               <  2:3321656/150‑1 (MQ=255)
       tACAGCCGCAAGACCAAGGAGCGGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCa                                                                                                                                              >  1:3838104/1‑150 (MQ=255)
        aCAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCAAAGCGGCCCCaa                                                                                                                                             <  1:2984791/150‑1 (MQ=255)
          aGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCAAAGCGGCCCCaaat                                                                                                                                           <  1:4385116/150‑3 (MQ=255)
              gCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCAttt                                                                                                                                       <  1:39271/150‑1 (MQ=255)
              gCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCAttt                                                                                                                                       >  1:3486590/1‑150 (MQ=255)
               cAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTa                                                                                                                                      <  2:945937/150‑1 (MQ=255)
                 aGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTatg                                                                                                                                    <  2:1378187/150‑1 (MQ=255)
                 aGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTatg                                                                                                                                    <  2:1875452/150‑1 (MQ=255)
                         gAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGat                                                                                                                            >  2:378240/1‑150 (MQ=255)
                         gAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGat                                                                                                                            <  1:1718428/150‑1 (MQ=255)
                             aGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATcc                                                                                                                        <  1:1592326/150‑1 (MQ=255)
                              gTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCg                                                                                                                       >  2:150429/1‑150 (MQ=255)
                              gTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCg                                                                                                                       >  1:3465071/1‑150 (MQ=255)
                              gTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTAGCGGCCCCAATCATTTATGATGCAGATATCCg                                                                                                                       <  2:2476020/150‑1 (MQ=255)
                               tATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGc                                                                                                                      <  2:4280054/150‑1 (MQ=255)
                                 tgtgCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCt                                                                                                                    >  2:2506289/1‑150 (MQ=255)
                                 tgtgCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCt                                                                                                                    <  1:3205974/150‑1 (MQ=255)
                                   tgCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTg                                                                                                                  >  1:2831668/1‑150 (MQ=255)
                                    gCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGt                                                                                                                 >  2:1668365/1‑150 (MQ=255)
                                        tCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGcc                                                                                                             <  2:3840394/150‑1 (MQ=255)
                                            aGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTAt                                                                                                         <  1:2196532/150‑1 (MQ=255)
                                              gTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATgg                                                                                                       >  1:2110392/1‑150 (MQ=255)
                                                 gATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAg                                                                                                    <  2:2201777/150‑1 (MQ=255)
                                                 gATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTGGAAGATAAGCGCTGATCCATGGGTGCTGTGAGTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTCTGGGAg                                                                                                    >  2:4430141/1‑150 (MQ=255)
                                                  aTGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAgg                                                                                                   <  1:1688177/150‑1 (MQ=255)
                                                  aTGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAgg                                                                                                   <  2:4011633/150‑1 (MQ=255)
                                                   tGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGTGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAggg                                                                                                  >  1:2953998/1‑150 (MQ=255)
                                                   tGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAggg                                                                                                  <  1:229391/150‑1 (MQ=255)
                                                       aaGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCGGATATCCGCCTTGTAGCCTTATGGGAGGGCACt                                                                                              >  2:39064/1‑150 (MQ=255)
                                                       aaGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACt                                                                                              <  2:4151554/150‑1 (MQ=255)
                                                        aGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTg                                                                                             <  2:403159/150‑1 (MQ=255)
                                                            gACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAAt                                                                                         <  1:2155551/150‑1 (MQ=255)
                                                            gACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAAt                                                                                         >  2:1940268/1‑150 (MQ=255)
                                                             aCCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATg                                                                                        <  1:38727/150‑1 (MQ=255)
                                                              gcGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATgg                                                                                       >  1:2042535/2‑150 (MQ=255)
                                                              ccGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATgg                                                                                       >  1:2042541/1‑150 (MQ=255)
                                                               cGGCTGGAAGGCATTCTACGACGGTAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGc                                                                                      >  2:986552/1‑150 (MQ=255)
                                                               cGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGc                                                                                      <  1:3581542/150‑1 (MQ=255)
                                                                  cTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCa                                                                                   >  1:4436634/1‑150 (MQ=255)
                                                                  cTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCa                                                                                   <  1:2944321/150‑1 (MQ=255)
                                                                  cTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCa                                                                                   <  1:3403206/150‑1 (MQ=255)
                                                                  cTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCa                                                                                   <  1:1347126/150‑1 (MQ=255)
                                                                  cTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCa                                                                                   <  2:3272427/150‑1 (MQ=255)
                                                                   tGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAg                                                                                  >  1:540404/1‑150 (MQ=255)
                                                                   tGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAg                                                                                  <  2:3663866/150‑1 (MQ=255)
                                                                     gAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGa                                                                                >  1:3060740/1‑150 (MQ=255)
                                                                        ggCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAAct                                                                             >  2:3751202/1‑150 (MQ=255)
                                                                        ggCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAAct                                                                             <  1:3699769/150‑1 (MQ=255)
                                                                           aTTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTa                                                                          <  2:74737/150‑1 (MQ=255)
                                                                            ttCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTac                                                                         >  1:636352/1‑150 (MQ=255)
                                                                            ttCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTac                                                                         >  1:228602/1‑150 (MQ=255)
                                                                             tCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTaca                                                                        <  1:846778/150‑1 (MQ=255)
                                                                                 cgacgGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCg                                                                    <  1:1295333/150‑1 (MQ=255)
                                                                                  gacgGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGt                                                                   >  2:2009662/1‑150 (MQ=255)
                                                                                   acgGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTa                                                                  <  2:2634920/150‑1 (MQ=255)
                                                                                   acgGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTa                                                                  >  1:2894120/1‑150 (MQ=255)
                                                                                     ggCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTacc                                                                >  2:2294847/1‑150 (MQ=255)
                                                                                        aaTGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTaccaac                                                             <  1:1570412/150‑1 (MQ=255)
                                                                                        aaTGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTACTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTaccaac                                                             <  2:3523721/150‑1 (MQ=255)
                                                                                         aTGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTaccaacc                                                            >  1:3999227/1‑150 (MQ=255)
                                                                                         aTGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTaccaacc                                                            >  1:542246/1‑150 (MQ=255)
                                                                                          tGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTaccaacca                                                           <  1:4140093/150‑1 (MQ=255)
                                                                                           gCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAg                                                          >  2:2666038/1‑150 (MQ=255)
                                                                                            ccTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGa                                                         <  1:267295/150‑1 (MQ=255)
                                                                                            ccTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGa                                                         <  2:3548053/150‑1 (MQ=255)
                                                                                            ccTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGa                                                         >  2:3071109/1‑150 (MQ=255)
                                                                                              tGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGaaa                                                       <  1:1038778/150‑1 (MQ=255)
                                                                                              tGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGaaa                                                       >  1:2421629/1‑150 (MQ=255)
                                                                                                      tGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGttctt                                               <  2:1218494/150‑1 (MQ=255)
                                                                                                         aGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGTTATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGc                                            <  1:984190/150‑1 (MQ=255)
                                                                                                                cgcTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTc                                     <  1:102696/150‑1 (MQ=255)
                                                                                                                   tGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGcc                                  <  2:4258790/150‑1 (MQ=255)
                                                                                                                   tGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGcc                                  <  2:4093020/150‑1 (MQ=255)
                                                                                                                     aTCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCt                                <  2:1092149/150‑1 (MQ=255)
                                                                                                                      tCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTc                               <  2:2371259/150‑1 (MQ=255)
                                                                                                                      tCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTc                               <  2:573133/150‑1 (MQ=255)
                                                                                                                         aTTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAg                            >  1:3375762/1‑150 (MQ=255)
                                                                                                                             gTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTc                                     >  1:3761080/1‑137 (MQ=255)
                                                                                                                             gTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCa                        >  2:1437679/1‑150 (MQ=255)
                                                                                                                             gTGCTGTGATGGGGGGCCGCTTTGCGGCCCCAATCCTTTATGATGCAGAGATCCGCCTTGTCGCCTTATGGGAGGGCACTGAACTGGCCCAGGACCGCGCGACGCGGGCCCACCAGACCGTGGGCGGCGCCGGCCTCGCCCTCGAGTccg                        >  2:4516306/1‑149 (MQ=255)
                                                                                                                                cTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATgg                     <  1:4253111/150‑1 (MQ=255)
                                                                                                                                  gtgATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGcc                   <  2:2906420/150‑1 (MQ=255)
                                                                                                                                  gtgATTTGGGGCCGCTGTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGcc                   <  2:1898325/150‑1 (MQ=255)
                                                                                                                                    gATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGCCaa                 >  1:3718977/1‑150 (MQ=255)
                                                                                                                                         ggggCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGCCAAGGCTg            <  1:2506289/150‑1 (MQ=255)
                                                                                                                                          gggCCGCAAAGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGCCAAGGCTGa           >  1:757065/1‑150 (MQ=255)
                                                                                                                                            gCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGCCAAGGCTGAAc         >  1:2641981/1‑150 (MQ=255)
                                                                                                                                               gCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGCCAAGGCTGAACaaa      <  1:1743175/150‑1 (MQ=255)
                                                                                                                                                 tttGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGCCAAGGCTGAACAAAGt    <  1:4086361/150‑1 (MQ=255)
                                                                                                                                                 tttGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGCCAAGGCTGAACAAAGt    <  2:3104567/150‑1 (MQ=255)
                                                                                                                                                   tGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGCCAAGGCTGAACAAAGTCa  <  2:1917475/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GATCCGCTACAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGCCAAGGCTGAACAAAGTCA  >  NC_002947/2445320‑2445616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑