BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	255,258	A→G	5.2%	intergenic (‑142/+21)	PP_0203 ← / ← PP_t74	dipeptidase/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	255,258	0	A	G	5.2%	632.6 / 14.7	192	intergenic (‑142/+21)	PP_0203/PP_t74	dipeptidase/tRNA‑Arg
Reads supporting (aligned to +/- strand): ref base A (91/91); new base G (3/7); total (94/98)
Fisher's exact test for biased strand distribution p-value = 3.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.79e-01

Only 100 of 196 total aligned reads displayed.

AACTGCATGGTTGTGCGCATTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGACGTCCATCGCCTTGAT  >  NC_002947/255109‑255405

aaCTGCATGGTTGTGCGCATTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCa                                                                                                                                                     <  2:1251041/150‑1 (MQ=255)

aaCTGCATGGTTGTGCGCATTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCa                                                                                                                                                     >  2:2634011/1‑150 (MQ=255)

   tGCATGGTTGTGCGCATTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGc                                                                                                                                                  >  1:700348/1‑150 (MQ=255)

   tGCATGGTTGTGCGCATTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGc                                                                                                                                                  >  1:455607/1‑150 (MQ=255)

      aTGGTTGTGCGCAGTCCTTTGCCACTCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCa                                                                                                                                               <  2:1738196/150‑1 (MQ=255)

        ggTTGTGCGCATTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAgg                                                                                                                                             <  1:2289889/150‑1 (MQ=255)

             tgCGCATTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCTGGGCCTGAGCCCGGGGCttt                                                                                                                                        <  1:1236123/150‑1 (MQ=255)

              gcgcATTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTa                                                                                                                                       >  1:814454/1‑150 (MQ=255)

              gcgcATTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTa                                                                                                                                       >  2:2749990/1‑150 (MQ=255)

              gcgcATTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTa                                                                                                                                       <  1:4049393/150‑1 (MQ=255)

              gcgcATTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTa                                                                                                                                       >  1:3083627/1‑150 (MQ=255)

               cgcATTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTAt                                                                                                                                      <  1:2603379/150‑1 (MQ=255)

               cgcAGTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCTGGGCCTGAGCCCGGGGCttttt                                                                                                                                      <  1:2748080/150‑3 (MQ=255)

                gcATTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCTGGGCCTGAGCCCGGGGCtttttc                                                                                                                                     <  1:1949683/150‑4 (MQ=255)

                gcATTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCTGGGCCTGAGCCCGGGGCtttttc                                                                                                                                     <  2:4358119/150‑4 (MQ=255)

                gcATTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTAtt                                                                                                                                     <  1:1969730/150‑1 (MQ=255)

                 cATTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTg                                                                                                                                    >  1:642139/1‑150 (MQ=255)

                  aTTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGa                                                                                                                                   >  1:2433708/1‑150 (MQ=255)

                  aTTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACAAATGACCCTTGTAACGGGCGTCCCCGAACGGTGACATGACCCACAAACGAAAAAGCCCCGGCCACAGCCCCAGGCCTTTATTGa                                                                                                                                   >  2:778486/1‑150 (MQ=255)

                    tCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCtttttcgttt                                                                                                                                 <  1:682189/150‑8 (MQ=255)

                      cTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATg                                                                                                                               <  1:335420/150‑1 (MQ=255)

                      cTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATg                                                                                                                               <  1:1041257/150‑1 (MQ=255)

                           ccAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCa                                                                                                                          >  2:1289137/1‑150 (MQ=255)

                           ccAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCa                                                                                                                          >  2:3555126/1‑150 (MQ=255)

                                 aTTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTcggc                                                                                                                    >  2:1327987/1‑150 (MQ=255)

                                     ttCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGa                                                                                                                >  2:4221605/1‑150 (MQ=255)

                                        aGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTc                                                                                                             <  1:2960110/150‑1 (MQ=255)

                                        aGGGTTACCCGTTCGGGAAACGGGAAGACACCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTc                                                                                                             <  2:3931132/150‑1 (MQ=255)

                                           gTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGaa                                                                                                          <  1:2093155/150‑1 (MQ=255)

                                                ccGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCAc                                                                                                     >  1:1967847/1‑150 (MQ=255)

                                                   ttCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGAc                                                                                                  >  1:3591937/1‑150 (MQ=255)

                                                       ggAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCt                                                                                              <  2:3245153/150‑1 (MQ=255)

                                                        gAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTg                                                                                             >  1:1062303/1‑150 (MQ=255)

                                                        gAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTg                                                                                             >  1:2423321/1‑150 (MQ=255)

                                                                 aGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAg                                                                                    >  1:3922487/1‑150 (MQ=255)

                                                                 aGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAg                                                                                    <  1:4481918/150‑1 (MQ=255)

                                                                  gACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAgg                                                                                   <  1:2133435/150‑1 (MQ=255)

                                                                   aCGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAggg                                                                                  <  2:2390554/150‑1 (MQ=255)

                                                                   aCGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAggg                                                                                  <  1:824803/150‑1 (MQ=255)

                                                                   aCGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAggg                                                                                  <  2:3983077/150‑1 (MQ=255)

                                                                   aCGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAggg                                                                                  <  1:1348660/150‑1 (MQ=255)

                                                                    cGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGc                                                                                 <  1:1679414/150‑1 (MQ=255)

                                                                     gCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCa                                                                                >  2:3487826/1‑150 (MQ=255)

                                                                     gCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCa                                                                                >  1:2803329/1‑150 (MQ=255)

                                                                      cccACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATTGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAg                                                                               <  1:1142016/150‑1 (MQ=255)

                                                                      cccACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAg                                                                               >  1:677142/1‑150 (MQ=255)

                                                                       ccACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGt                                                                              >  2:3762133/1‑150 (MQ=255)

                                                                       ccACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGt                                                                              >  2:2463739/1‑150 (MQ=255)

                                                                        cACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTa                                                                             >  1:1935449/1‑150 (MQ=255)

                                                                        cACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCCTCCGAAGGCAggg                                                                             <  2:3904572/150‑3 (MQ=255)

                                                                         aCGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTAc                                                                            >  2:3878753/1‑150 (MQ=255)

                                                                         aCGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTAc                                                                            >  2:3665429/1‑150 (MQ=255)

                                                                             tAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTa                                                                        >  2:411784/1‑150 (MQ=255)

                                                                               acacCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATc                                                                      <  2:1309895/150‑1 (MQ=255)

                                                                                 acCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCTGGGCCTGAGCCCGGGGCtttttc                                                                                                                                     <  2:2561143/85‑4 (MQ=25)

                                                                                 acCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCTGGGCCTGAGCCCGGGGCtttttc                                                                                                                                     >  1:2561143/1‑82 (MQ=25)

                                                                                  ccAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAg                                                                   <  1:58992/150‑1 (MQ=255)

                                                                                   cAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGc                                                                  <  1:4229146/150‑1 (MQ=255)

                                                                                    aaTGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCt                                                                 >  1:2703423/1‑150 (MQ=255)

                                                                                      tGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGa                                                               <  2:1662375/150‑1 (MQ=255)

                                                                                            gTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTCTCCAGCTGAGCTACg                                                         >  1:3600914/1‑150 (MQ=255)

                                                                                             tGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGa                                                        <  1:1463795/150‑1 (MQ=255)

                                                                                             tGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGACTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGa                                                        <  1:382822/150‑1 (MQ=255)

                                                                                              ggTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAg                                                       <  2:1790743/150‑1 (MQ=255)

                                                                                                    ggAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAAc                                                 <  2:296696/150‑1 (MQ=255)

                                                                                                    ggAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAAc                                                 >  1:3034578/1‑150 (MQ=255)

                                                                                                    ggAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAAc                                                 >  1:2731318/1‑150 (MQ=255)

                                                                                                    ggAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAAc                                                 <  1:3567559/150‑1 (MQ=255)

                                                                                                           cAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGcgcacg                                          >  2:968251/1‑150 (MQ=255)

                                                                                                           cAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGcgcacg                                          >  1:460332/1‑150 (MQ=255)

                                                                                                            aGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGcgcacgc                                         >  1:3279520/1‑150 (MQ=255)

                                                                                                            aGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGcgcacgc                                         <  1:1360154/150‑1 (MQ=255)

                                                                                                            aGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGcgcacgc                                         >  2:1039124/1‑150 (MQ=255)

                                                                                                             gAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGcgcacgca                                        <  2:1994633/150‑1 (MQ=255)

                                                                                                              aaaGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCAt                                       <  2:4090260/150‑1 (MQ=255)

                                                                                                                aGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGa                                     <  2:680142/150‑1 (MQ=255)

                                                                                                                  gTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATa                                   >  1:867656/1‑150 (MQ=255)

                                                                                                                  gTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATa                                   >  2:1884305/1‑150 (MQ=255)

                                                                                                                   tGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATAc                                  >  2:4474777/1‑150 (MQ=255)

                                                                                                                      cATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCa                               <  1:1503342/150‑1 (MQ=255)

                                                                                                                         gTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCt                            <  2:4166610/150‑1 (MQ=255)

                                                                                                                            gCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCtgtg                         <  1:42025/150‑1 (MQ=255)

                                                                                                                             cacaAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTCTCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCCTCTgtgt                        >  1:1327258/1‑150 (MQ=255)

                                                                                                                             cacaAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTgtgt                        >  2:369652/1‑150 (MQ=255)

                                                                                                                             cacaAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTgtgt                        <  2:700348/150‑1 (MQ=255)

                                                                                                                              acaAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTc                       >  1:4246199/1‑150 (MQ=255)

                                                                                                                                aaaCGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGt                     >  1:1077584/1‑150 (MQ=255)

                                                                                                                                 aaCGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGtt                    >  1:2847201/1‑150 (MQ=255)

                                                                                                                                  aCGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTg                   >  1:1745529/1‑150 (MQ=255)

                                                                                                                                  aCGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCAGGATACCCATCTGTGTCGTTg                   >  1:1768273/1‑150 (MQ=255)

                                                                                                                                    gAAAATGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGAc                 >  2:490713/1‑150 (MQ=255)

                                                                                                                                    gAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGAc                 >  2:2311957/1‑150 (MQ=255)

                                                                                                                                    aataaaGCCCTGGGCCTGAGCCCGGGGCTTTCTTGATATGGCGCACTCGGCGGGCTGCGAACCCACGACCCCTGCCGTCGGAGGGCAGGACTCTCTCCAGCTGAGCTACGAGTGCCACGCGCACGCATGAGACCCATCGGTGTCGATGAc                 >  2:3601933/4‑150 (MQ=255)

                                                                                                                                      aaaaGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGACGt               >  2:4337700/1‑150 (MQ=255)

                                                                                                                                      aaaaGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGACGt               <  2:1679773/150‑1 (MQ=255)

                                                                                                                                           ccccGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGACGTCCATc          >  1:558450/1‑150 (MQ=255)

                                                                                                                                            cccGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGACGTCCATCg         >  1:3710257/1‑150 (MQ=255)

                                                                                                                                                ggCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGACGTCCATCGCCtt     <  2:3239023/150‑1 (MQ=255)

                                                                                                                                                   tCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGACGTCCATCGCCTTGAt  >  2:4065451/1‑150 (MQ=255)

                                                                                                                                                   tCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGACGTCCATCGCCTTGAt  <  1:4103156/150‑1 (MQ=255)

AACTGCATGGTTGTGCGCATTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGACGTCCATCGCCTTGAT  >  NC_002947/255109‑255405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`