BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,875,612	G→A	6.8%	V143M (GTG→ATG) ‡	cigR →	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,875,612	0	G	A	6.8%	505.5 / 17.4	162	V143M (GTG→ATG) ‡	cigR	membrane protein
Reads supporting (aligned to +/- strand): ref base G (71/79); new base A (7/4); total (78/84)
Fisher's exact test for biased strand distribution p-value = 3.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 164 total aligned reads displayed.

GCCTCCAGGCATTCAGAAGAACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCAGCAGCCCAACGGCCTATCAACGCTGCCGTAACACCGC  >  NC_002947/2875464‑2875761

gCCTCCAGGCATTCAGAAGAACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTAGGAGTGGATGCAAGCGGGCGCAGACCTGAGTCTGGTCGCCGt                                                                                                                                                      >  2:4133891/1‑150 (MQ=255)

 ccTCCAGGCATTCAGAAGAACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTg                                                                                                                                                     <  2:786581/150‑1 (MQ=255)

 ccTCCAGGCATTCAGAAGAACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTg                                                                                                                                                     <  2:423171/150‑1 (MQ=255)

  cTCCAGGCATTCAGAAGAACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTgg                                                                                                                                                    <  2:2741477/150‑1 (MQ=255)

     cAGGCATTCAGAAGAACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGATGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGa                                                                                                                                                 >  1:3858262/1‑150 (MQ=255)

     cAGGCATTCAGAAGAACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGa                                                                                                                                                 >  1:4313960/1‑150 (MQ=255)

     cAGGCATTCAGAAGAACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGa                                                                                                                                                 <  2:2224525/150‑1 (MQ=255)

     cAGGCATTCAGAAGAACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGa                                                                                                                                                 >  1:1389374/1‑150 (MQ=255)

     cAGGCATTCAGAAGAACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGa                                                                                                                                                 >  2:3682429/1‑150 (MQ=255)

            tCAGAAGAACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCACGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCACGCGGGCGCAGACCTGATGCTGGTCGCCGTGGCGACCGGGat                                                                                                                                          >  2:2684395/1‑150 (MQ=255)

              agaagaACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGatca                                                                                                                                        >  1:803451/1‑150 (MQ=255)

              agaagaACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGatca                                                                                                                                        >  2:3879494/1‑150 (MQ=255)

               gaagaaCCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATCCCGGTCGCCACGGCGACCAGAatcag                                                                                                                                       <  2:4001653/150‑2 (MQ=255)

                 agaaCCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCt                                                                                                                                     <  2:763669/150‑1 (MQ=255)

                    aCCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACAGGGATCATCTAtt                                                                                                                                  <  1:978171/150‑2 (MQ=255)

                     ccTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGa                                                                                                                                 <  2:2870503/150‑1 (MQ=255)

                      cTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAg                                                                                                                                >  2:2354257/1‑150 (MQ=255)

                      cTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAg                                                                                                                                <  2:3327688/150‑1 (MQ=255)

                      cTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAg                                                                                                                                <  2:3339205/150‑1 (MQ=255)

                        ggCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGgt                                                                                                                              >  1:1847702/1‑150 (MQ=255)

                        ggCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGgt                                                                                                                              >  1:1847681/1‑150 (MQ=255)

                         gCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGgtg                                                                                                                             <  1:1276019/150‑1 (MQ=255)

                                gCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACg                                                                                                                      >  1:1829409/1‑150 (MQ=255)

                                gCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACg                                                                                                                      >  1:2927844/1‑150 (MQ=255)

                                 cAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACgg                                                                                                                     >  2:16627/1‑150 (MQ=255)

                                 cAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACgg                                                                                                                     <  2:2565052/150‑1 (MQ=255)

                                  aaGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACggg                                                                                                                    <  1:3106009/150‑1 (MQ=255)

                                  aaGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACggg                                                                                                                    >  1:400643/1‑150 (MQ=255)

                                  aaGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACggg                                                                                                                    <  2:1866922/150‑1 (MQ=255)

                                        cTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTc                                                                                                              >  1:572626/1‑150 (MQ=255)

                                          gCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGa                                                                                                            <  1:1054819/150‑1 (MQ=255)

                                          gCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGa                                                                                                            <  1:38085/150‑1 (MQ=255)

                                          gCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGa                                                                                                            <  1:3143175/150‑1 (MQ=255)

                                          gCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGa                                                                                                            <  2:996397/150‑1 (MQ=255)

                                          gCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGa                                                                                                            <  1:1417987/150‑1 (MQ=255)

                                          gCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGa                                                                                                            <  1:2575726/150‑1 (MQ=255)

                                           ccACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGAc                                                                                                           <  2:2974676/150‑1 (MQ=255)

                                             aCCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACAACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTa                                                                                                         >  1:654223/1‑150 (MQ=255)

                                              ccTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTaa                                                                                                        <  2:2296134/150‑1 (MQ=255)

                                               cTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAg                                                                                                       <  1:491577/150‑1 (MQ=255)

                                                tGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAgg                                                                                                      <  1:3609877/150‑1 (MQ=255)

                                                 ggTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGc                                                                                                     <  1:404360/150‑1 (MQ=255)

                                                 ggTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGc                                                                                                     <  2:572626/150‑1 (MQ=255)

                                                     tCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCa                                                                                                 <  1:2935639/150‑1 (MQ=255)

                                                       gCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTGGAACGGGGCATTCGACTAAGGCCGCAgg                                                                                               >  2:2550156/1‑150 (MQ=255)

                                                          aagaagCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGc                                                                                            >  2:1473992/1‑150 (MQ=255)

                                                          aagaagCTGGATGGTCGTCTGTTGGGGCAGCGGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGc                                                                                            <  1:2714330/150‑1 (MQ=255)

                                                            gaagCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGa                                                                                          <  1:765441/150‑1 (MQ=255)

                                                            gaagCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGa                                                                                          <  1:1281538/150‑1 (MQ=255)

                                                            gaagCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGAGGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGa                                                                                          <  1:2032340/150‑1 (MQ=255)

                                                                   gATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTcc                                                                                   >  1:170053/1‑150 (MQ=255)

                                                                      ggTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCt                                                                                <  1:2749540/150‑1 (MQ=255)

                                                                       gtcgtcTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAATGCCGCAGGTGCGAGCAGTCCGCTc                                                                               <  2:3791877/150‑1 (MQ=255)

                                                                       gtcgtcTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTc                                                                               >  1:4213962/1‑150 (MQ=255)

                                                                          gtcTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGt                                                                            >  2:1179709/1‑150 (MQ=255)

                                                                           tcTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTc                                                                           >  2:2494714/1‑150 (MQ=255)

                                                                            cTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAg                                                                                         <  2:3236741/135‑1 (MQ=255)

                                                                            cTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTcc                                                                          >  2:1606155/1‑150 (MQ=255)

                                                                             tGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTccc                                                                         <  2:3628575/150‑1 (MQ=255)

                                                                              gTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCt                                                                        >  2:1607559/1‑150 (MQ=255)

                                                                                     cAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGgcg                                                                 <  2:664397/150‑1 (MQ=255)

                                                                                      aGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGgcgc                                                                >  2:4035960/1‑150 (MQ=255)

                                                                                      aGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGgcgc                                                                >  2:1761406/1‑150 (MQ=255)

                                                                                      aGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGgcgc                                                                >  2:4035946/1‑150 (MQ=255)

                                                                                       gCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCt                                                               >  1:4338733/1‑150 (MQ=255)

                                                                                        cggCCTCACTACGACGGGTATGAGTGGCTGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTg                                                              <  1:163633/148‑1 (MQ=255)

                                                                                             tCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTg                                                         >  2:2286532/1‑150 (MQ=255)

                                                                                                  acgacgGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGc                                                    <  1:3655834/150‑1 (MQ=255)

                                                                                                  acgacgGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGc                                                    <  2:505638/150‑1 (MQ=255)

                                                                                                  acgacgGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGc                                                    <  1:2261093/150‑1 (MQ=255)

                                                                                                    ggcgGGTAGGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTAGGAGGTGTTGAACGGGGCAGTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGa                                                  >  1:2799821/3‑150 (MQ=255)

                                                                                                    gacgGGTATGAGTGGAGGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGa                                                  <  2:3076440/150‑1 (MQ=255)

                                                                                                     acgGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGAt                                                 <  1:2628466/150‑1 (MQ=255)

                                                                                                      cgGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATg                                                <  2:3483198/150‑1 (MQ=255)

                                                                                                      cgGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATg                                                <  2:1817400/150‑1 (MQ=255)

                                                                                                      cgGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATg                                                <  2:855947/150‑1 (MQ=255)

                                                                                                               gTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCgca                                       >  2:2623932/1‑150 (MQ=255)

                                                                                                                 ggATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGTTGGGCTgctgcgc                                     <  2:2305064/150‑6 (MQ=255)

                                                                                                                 ggATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGTTGGGCTgctgcgc                                     <  2:2305021/150‑6 (MQ=255)

                                                                                                                  gATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCgcagca                                    >  2:543980/1‑150 (MQ=255)

                                                                                                                    tGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCagcagc                                  <  2:3904250/150‑1 (MQ=255)

                                                                                                                       aaGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCTGCGCAGCCCa                               <  1:1735345/150‑1 (MQ=255)

                                                                                                                         gCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGTTGGGCTGCTGCGCAGCCCatc                             <  1:2307675/150‑3 (MQ=255)

                                                                                                                         gCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCAGCAGCCCAAc                             >  1:639535/1‑150 (MQ=255)

                                                                                                                         gCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCGATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCGCGGTCCCTGCAGGCGCTGGCGTGCCTGCGCTGGGCTGCGCAGCAGcccgcc                             >  1:1068062/1‑147 (MQ=255)

                                                                                                                           gggCGCAGACCTTATTCTGGTCGCGGTGGCGACGGGTATCTTTTATGAGGTGTTGAACGGGGCTTTCGACTAAGGCCGCAGTTGCGAGCAGTCCGCTGGGTCCCTGCTGGCGCTGGGGTGCCTGCTTTGGGCTGCGCAGCAGCCCAACgg                           <  2:1236319/150‑1 (MQ=255)

                                                                                                                               tcatagatGATCCCGGTCGCCACGGCGACCAGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCAGCAGCCCAACGGCCTa                       >  1:2602281/8‑150 (MQ=255)

                                                                                                                               gcAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGc                                             >  2:1677739/1‑128 (MQ=255)

                                                                                                                                 aGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCAGCAGCCCAACGGCCTATc                     >  1:213453/1‑150 (MQ=255)

                                                                                                                                     cTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCAGCAGCCCAACGGCCTATCAACg                 >  1:3097020/1‑150 (MQ=255)

                                                                                                                                     cTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCAGCAGCCCAACGGCCTATCAACg                 >  1:369065/1‑150 (MQ=255)

                                                                                                                                         ttCTGGTCGCCGTGGCGACCGGGGTCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCAGCAGCCCAACGGCCTATCAACGCTGc             >  1:1983563/1‑150 (MQ=255)

                                                                                                                                         tcccggTCGCCACGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCCGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCAGCAGCCCAACGGCCTATCAACGCTGc             >  1:2837089/5‑150 (MQ=255)

                                                                                                                                         tcccggTCGCCACGGCGACCAGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCAGCAGCCCAACGGCCTATCAACGCTGc             >  2:921121/5‑150 (MQ=255)

                                                                                                                                         tcccggTCGCCACGGCGACCAGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCAGCAGCCCAACGGCCTATCAACGCTGc             >  2:921172/5‑150 (MQ=255)

                                                                                                                                          tCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCAGCAGCCCAACGGCCTATCAACGCTGcc            >  2:1771491/1‑150 (MQ=255)

                                                                                                                                            tGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCAGCAGCCCAACGGCCTATCAACGCTGCCGt          >  2:1762463/1‑150 (MQ=255)

                                                                                                                                            tGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCAGCAGCCCAACGGCCTATCAACGCTGCCGt          >  2:1762523/1‑150 (MQ=255)

                                                                                                                                            cggTCGCCACGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCAGCAGCCCAACGGCCTATCAACGCTGCCGt          >  1:1293362/2‑150 (MQ=255)

                                                                                                                                                    gTGGCGCCCGGTATCATCTTTGAGGGGTAGAACGGGGCTTTCCCCGTAGTCCGCAGGTGCGAGCACGCCGCCCGGTCCCTGCTGGCGCGGGGCTGCCTGCGTTGGGCTGCGCTGCAGCCCCACGGCCTATCAACGCTGCCGTAACACcgc  <  2:2955417/150‑1 (MQ=255)

GCCTCCAGGCATTCAGAAGAACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGCGCAGCAGCCCAACGGCCTATCAACGCTGCCGTAACACCGC  >  NC_002947/2875464‑2875761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`