Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,012,941 C→A 7.0% R13S (CGC→AGC)  bcsG → endoglucanase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,012,9410CA 7.0% 704.4 / 29.9 209R13S (CGC→AGC) bcsGendoglucanase
Reads supporting (aligned to +/- strand):  ref base C (100/93);  new base A (2/13);  total (102/107)
Fisher's exact test for biased strand distribution p-value = 5.65e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 210 total aligned reads displayed.

TTGCTAGTGCGCCTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTT‑CCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGTGGCTGCGTATTGCC  >  NC_002947/3012793‑3013090
                                                                                                                                                     |                                                                                                                                                     
ttgCTAGTGCGCCTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTT‑CCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCg                                                                                                                                                      >  1:974430/1‑150 (MQ=255)
  gCTAGTGCGCCTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTT‑CCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCt                                                                                                                                                    >  1:4273073/1‑150 (MQ=255)
  gCTAGTGCGCCTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTT‑CCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCt                                                                                                                                                    >  1:2004266/1‑150 (MQ=255)
       tGCGCCTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTT‑CCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCt                                                                                                                                               <  2:2485125/150‑1 (MQ=255)
            cTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTT‑CCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACt                                                                                                                                          >  2:2936860/1‑150 (MQ=255)
            cTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTT‑CCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACt                                                                                                                                          <  1:3133468/150‑1 (MQ=255)
            cTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTT‑CCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCAGTCCAGGCCAGCGGGccagtacc                                                                                                                                          <  2:3734556/150‑7 (MQ=255)
             tgtgTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTT‑CCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCAGTCCAGGCCAGCGGGccagtaccg                                                                                                                                         <  1:3218228/150‑8 (MQ=255)
              gtgtTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTT‑CCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTgg                                                                                                                                        <  1:2903083/150‑1 (MQ=255)
              gtgtTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTT‑CCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACCgg                                                                                                                                        <  2:2971981/150‑1 (MQ=255)
                gtTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTT‑CCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGa                                                                                                                                      <  2:1750314/150‑1 (MQ=255)
                gtTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTT‑CCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCAGTCCAGGCCAGCGGGCCAGTACCGGGa                                                                                                                                      <  2:49738/150‑1 (MQ=38)
                 ttCGGCATTACGTGGCGTGTTGCGCCGTCGCCTT‑CCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTgg                                                                                                                                                  >  2:338442/1‑137 (MQ=255)
                 ttCGGCATTACGTGGCGTGTTGCGCCGTCGCCTT‑CCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAg                                                                                                                                     <  2:2276492/150‑1 (MQ=255)
                  tCGGCATTACGTGGCGTGTTGCGCCGTCGCCTT‑CCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAgg                                                                                                                                    <  1:2043563/150‑1 (MQ=255)
                  tCGGCATTACGTGGCGTGTTGCGCCGTCGCCTT‑CCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAgg                                                                                                                                    <  2:407939/150‑1 (MQ=255)
                  tCGGCATTACGTGGCGTGTTGCGCCGTCGCCTT‑CCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCACCACCCTCCCAGTCCAGGCCAGCGGGCCAGTACCGGGAgg                                                                                                                                    <  2:1329721/150‑1 (MQ=38)
                    ggCATTACGTGGCGTGTTGCGCCGTCGCCTT‑CCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGt                                                                                                                                  >  1:1798506/1‑150 (MQ=255)
                       aTTACGTGGCGTGTTGCGCCGTCGCCTT‑CCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGa                                                                                                                               >  1:1857419/1‑150 (MQ=255)
                        ttACGTGGCGTGTTGCGCCGTCGCCTT‑CCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGaa                                                                                                                              >  2:1516144/1‑150 (MQ=255)
                         tACGTGGCGTGTTGCGCCGTCGCCTT‑CCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAAc                                                                                                                             >  1:2168553/1‑150 (MQ=255)
                           cGTGGCGTGTTGCGCCGTCGCCTT‑CCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACct                                                                                                                           <  2:170579/150‑1 (MQ=255)
                            gTGGCGTGTTGCGCCGTCGCCTT‑CCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACctc                                                                                                                          >  1:2422066/1‑150 (MQ=255)
                            gTGGCGTGTTGCGCCGTCGCCTT‑CCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACctc                                                                                                                          <  1:2152206/150‑1 (MQ=255)
                             tGGCGTGTTGCGCCGTCGCCTT‑CCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACctct                                                                                                                         >  2:3879655/1‑150 (MQ=255)
                             tGGCGTGTTGCGCCGTCGCCTT‑CCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACctct                                                                                                                         >  1:1203317/1‑150 (MQ=255)
                             tGGCGTGTTGCGCCGTCGCCTT‑CCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACctct                                                                                                                         <  1:2510541/150‑1 (MQ=255)
                              ggCGTGTTGCGCCGTCGCCTT‑CCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTa                                                                                                                        <  1:2795634/150‑1 (MQ=255)
                                   gtTGCGCCGTCGCCTT‑CCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTAttttt                                                                                                                   >  2:1084343/1‑150 (MQ=255)
                                     tGCGCCGTCGCCTT‑CCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTCATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTAttttttt                                                                                                                 <  1:2694037/150‑1 (MQ=255)
                                     tGCGCCGTCGCCTT‑CCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTAttttttt                                                                                                                 <  1:1503037/150‑1 (MQ=255)
                                     tGCGCCGTCGCCTT‑CCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTAttttttt                                                                                                                 <  1:1821435/150‑1 (MQ=255)
                                     tGCGCCGTCGCCGT‑CCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTAttttttt                                                                                                                 <  2:485326/150‑1 (MQ=255)
                                      gcgcCGTCGCCTTCCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGATCCTCTAttttttt                                                                                                                 <  2:2351794/150‑1 (MQ=255)
                                          cGTCGCCTT‑CCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCaaa                                                                                                            <  2:3745541/150‑1 (MQ=255)
                                          cGTCGCCTT‑CCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCaaa                                                                                                            >  1:3141225/1‑150 (MQ=255)
                                            tCGCCTT‑CCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGt                                                                                                          >  2:4514499/1‑150 (MQ=255)
                                                cTT‑CCCCCCCGGTACCTGAATCCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTct                                                                                                      <  2:334054/150‑1 (MQ=255)
                                                 tt‑CCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTctg                                                                                                     <  1:3345515/150‑1 (MQ=255)
                                                    cccccccGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTctgct                                                                                                   <  1:3915270/150‑1 (MQ=255)
                                                      cccccGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTgg                                                                                                 >  2:2229152/1‑150 (MQ=255)
                                                      cccccGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTgg                                                                                                 >  2:2877103/1‑150 (MQ=255)
                                                      cccccGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCGCTATTTTTTTGCACAGGTTCTGCTgg                                                                                                 >  2:3339574/1‑150 (MQ=255)
                                                               ccTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGACTGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTgg                                                                                        <  2:694420/150‑1 (MQ=255)
                                                                cTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTggg                                                                                       >  1:1285767/1‑150 (MQ=255)
                                                                 tGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGc                                                                                      >  2:4464287/1‑150 (MQ=255)
                                                                  gAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCa                                                                                     >  2:3941034/1‑150 (MQ=255)
                                                                     aCCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGc                                                                                  >  1:1755600/1‑150 (MQ=255)
                                                                      ccGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCt                                                                                 <  1:1067311/150‑1 (MQ=255)
                                                                        gCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCg                                                                               >  2:2073405/1‑150 (MQ=255)
                                                                        gCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAAACTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCg                                                                               >  2:2033104/1‑150 (MQ=255)
                                                                          tGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGAc                                                                             >  1:2985512/1‑150 (MQ=255)
                                                                              cGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTcc                                                                         <  1:489206/150‑1 (MQ=255)
                                                                                 aCGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAgg                                                                      <  2:1429636/150‑1 (MQ=255)
                                                                                   gccgccgcGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGgcg                                                                    >  1:4414995/1‑150 (MQ=255)
                                                                                   gccgccgcGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGgcg                                                                    >  2:2192696/1‑150 (MQ=255)
                                                                                     cgccgcGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCt                                                                  >  1:3262412/1‑150 (MQ=255)
                                                                                     cgccgcGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCt                                                                  >  1:1556745/1‑150 (MQ=255)
                                                                                      gccgcGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTg                                                                 <  1:2936860/150‑1 (MQ=255)
                                                                                        cgcgCTTCACGCCAGTAACCCCAGAATGACTAATACCACCATCTCCCTCCCGGTTCTGGCCCGATGGCCTGGACTGGGAGGGTGTAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGAGCTGGGCATGCTCGACTTCCAGGCGCTGcc                                                               <  2:3977768/150‑1 (MQ=255)
                                                                                        cgcgCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGcc                                                               <  2:3260136/150‑1 (MQ=255)
                                                                                            cTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAAc                                                           <  1:222768/150‑1 (MQ=255)
                                                                                              gCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCAAACCt                                                         <  2:1191689/150‑1 (MQ=255)
                                                                                                  gCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGACCCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCtgttg                                                     >  1:583281/1‑150 (MQ=255)
                                                                                                    cAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTgttgtt                                                   >  2:612753/1‑150 (MQ=255)
                                                                                                    cAGGAACCCCAGAATGACTAATACCAACATCACCCTCCCGGTACTGGCCCTCTGGCATGGACTGGGATGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGAATTCTCGACTTCCAGGCGCTGCCGAACATgttgtt                                                   <  1:355480/150‑1 (MQ=255)
                                                                                                     aGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTc                                                  <  1:3095171/150‑1 (MQ=255)
                                                                                                       gAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGc                                                <  1:653024/150‑1 (MQ=255)
                                                                                                         aCCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGGTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTg                                              <  1:918899/150‑1 (MQ=255)
                                                                                                          ccccAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGATGc                                             >  1:3970176/1‑150 (MQ=255)
                                                                                                                aaTGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCt                                       <  1:4439386/150‑1 (MQ=255)
                                                                                                                  tGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGc                                     <  1:2110588/150‑1 (MQ=255)
                                                                                                                  tGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGc                                     <  2:3780215/150‑1 (MQ=255)
                                                                                                                  tGACTAAGACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGc                                     <  2:3827679/150‑1 (MQ=255)
                                                                                                                    aCTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCtt                                   >  1:1194372/1‑150 (MQ=255)
                                                                                                                    aCTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCtt                                   <  2:4353207/150‑1 (MQ=255)
                                                                                                                     cTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTg                                  >  1:3241716/1‑150 (MQ=255)
                                                                                                                     cTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTg                                  >  2:2795542/1‑150 (MQ=255)
                                                                                                                      tAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGt                                 >  1:3197648/1‑150 (MQ=255)
                                                                                                                      tAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGt                                 <  2:4196989/150‑1 (MQ=255)
                                                                                                                        aTACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTcc                               >  1:3395310/1‑150 (MQ=255)
                                                                                                                        aTACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTcc                               <  1:1342761/150‑1 (MQ=255)
                                                                                                                        aTACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTcc                               >  1:1580196/1‑150 (MQ=255)
                                                                                                                         tACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTccc                              >  1:4158671/1‑150 (MQ=255)
                                                                                                                            caccaTCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCttt                           >  1:4240095/1‑150 (MQ=255)
                                                                                                                            caccaTCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCttt                           <  2:2827530/150‑1 (MQ=255)
                                                                                                                              ccaTCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGc                                                                                      >  1:3635173/1‑89 (MQ=255)
                                                                                                                              ccaTCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGc                                                                                      <  2:3635173/89‑1 (MQ=255)
                                                                                                                               ttccACCCTCCCAGTCCAGGCCAGCGGGCCAGTACCGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGcc                        >  1:1815472/4‑150 (MQ=37)
                                                                                                                                 tCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCgg                      >  1:1022957/1‑150 (MQ=255)
                                                                                                                                   aCCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGc                    <  2:2197374/150‑1 (MQ=255)
                                                                                                                                    cccTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCa                   >  1:1306339/1‑150 (MQ=255)
                                                                                                                                      cTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAg                 >  2:890820/1‑150 (MQ=255)
                                                                                                                                        cccGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGTg               <  1:2301347/150‑1 (MQ=255)
                                                                                                                                         ccGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGTgg              <  1:2877103/150‑1 (MQ=255)
                                                                                                                                         ccGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGTgg              >  2:3359604/1‑150 (MQ=255)
                                                                                                                                                tGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGTGGCTGCGTa       <  2:523384/150‑1 (MQ=255)
                                                                                                                                                tGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAAACTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGTGGCTGCGTa       >  2:1262266/1‑150 (MQ=255)
                                                                                                                                                tGGCCAGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTATTTGCAAAGTTACTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCCCTGCCGAACCGGTTGTACGCTGCGTTTCAGCTTGTCCCTATGCCGGGCAAGTGGCTGCGTa       >  2:305872/1‑150 (MQ=255)
                                                                                                                                                  gCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGTGGCTGCGTAtt     <  1:2556307/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTGCTAGTGCGCCTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTT‑CCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGTGGCTGCGTATTGCC  >  NC_002947/3012793‑3013090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑