Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,255,352 C→T 5.5% A19T (GCT→ACT)  PP_2851 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,255,3520CT 5.5% 588.3 / 15.7 181A19T (GCT→ACT) PP_2851hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (83/88);  new base T (6/4);  total (89/92)
Fisher's exact test for biased strand distribution p-value = 5.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 188 total aligned reads displayed.

GAACAGGTCGGCCAGACTCAACGCGCCATTTGGCCCGAAGAAGCGGTAATCCTGCTCGACCTTTACCCAAGGCAGGGCGCGACGGGCTGCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTCACACCTTGGTCGAGTACGACCAGGTCGATTCGACAAGCACCGGTGGCCGGGCGATGAGCGGTATCTGCT  >  NC_002947/3255203‑3255501
                                                                                                                                                     |                                                                                                                                                       
gAACAGGTCGGCCAGACTCAACGCGCCATTTGGCCCGAAGAAGCGGTAATCCTGCTCGACCTTTACCCAAGGCAGGGCGCGACGGGCTGCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAgc                                                                                                                                                         >  2:1671826/1‑150 (MQ=255)
    aGGTCGGCCAGACTCAACGCGCCATTTGGCCCGAAGAAGCGGTAATCCTGCTCGACCTTTACCCAAGGCAGGGCGCGACGGGCTGCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAgccgcc                                                                                                                                                     >  1:2911179/1‑150 (MQ=255)
         ggCCAGACTCAACGCGCCATTTGGCCCGAAGAAGCGGTAATCCTGCTCGACCTTTACCCAAGGCAGGGCGCGACGGGCTGCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCgccagcca                                                                                                                                                <  2:1721660/150‑1 (MQ=255)
          gCCAGACTCAACGCGCCATTTGGCCCGAAGAAGCGATAATCCTGCTCGACCTTTACCCAAGGCAGGGCGCGACGGGCTGCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTGGCTGGCGGCTCGCCAGCAAc                                                                                                                                               <  1:1376191/150‑1 (MQ=255)
           ccAGACTCAACGCGCCATTTGGCCCGAAGAAGCGGTAATCCTGCTCGACCTTTACCCAAGGCAGGGCGCGACGGGCTGCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑t                                                                                                                                            >  1:750493/1‑150 (MQ=255)
             aGACTCAACGCGCCATTTGGCCCGAAGAAGCGGTAATCCTGCTCGACCTTTACCCAAGGCAGGGCGCGACGGGCTGCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑Tgg                                                                                                                                          >  1:3122257/1‑150 (MQ=255)
             aGACTCAACGCGCCATTTGGCCCGAAGAAGCGGTAATCCTGCTCGACCTTTACCCAAGGCAGGGCGCGACGGGCTGCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTGGCTGGCGGCTCGCCAGCAAC‑‑Tgt                                                                                                                                          <  2:727663/150‑2 (MQ=255)
                 tCAACGCGCCATTTGGCCCGAAGAAGCGGTAATCCTGCTCGACCTTTACCCAAGGCAGGGCGCGACGGGCTGCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGc                                                                                                                                      >  2:2564771/1‑150 (MQ=255)
                 tCAACGCGCCATTTGGCCCGAAGAAGCGGTAATCCTGCTCGACCTTTACCCAAGGCAGGGCGCGACGGGCTGCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGc                                                                                                                                      <  2:3688800/150‑1 (MQ=255)
                   aaCGCGCCATTTGGCCCGAAGAAGCGGTAATCCTGCTCGACCTTTACCCAAGGCAGGGCGCGACGGGCTGCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCgg                                                                                                                                    <  2:393166/150‑1 (MQ=255)
                   aaCGCGCCATTTGGCCCGAAGAAGCGGTAATCCTGCTCGACCTTTACCCAAGGCAGGGCGCGACGGGCTGCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCgg                                                                                                                                    >  2:1639500/1‑150 (MQ=255)
                      gcgcCATTTGGCCCGAAGAAGCGGTAATCCTGCTCGACCTTTACCCAAGGCAGGGCGCGACGGGCTGCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGaa                                                                                                                                 <  1:148394/150‑1 (MQ=255)
                      gcgcCATTTGGCCCGAAGAAGCGGTAATCCTGCTCGACCTTTACCCAAGGCAGGGCGCGACGGGCTGCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGaa                                                                                                                                 <  2:3640552/150‑1 (MQ=255)
                       cgcCATTTGGCCCGAAGAAGCGGTAATCCTGCTCGACCTTTACCCAAGGCAGGGCGCGACGGGCTGCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGaaa                                                                                                                                <  1:156730/150‑1 (MQ=255)
                       cgcCATTGGGCCCGAAGAAGCGGTAATCCTGCTCGACCTTTACCCAAGGCAGGGCGCGACGGGCTGCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGaaa                                                                                                                                <  1:2414451/150‑1 (MQ=255)
                        gcCATTTGGCCCGAAGAAGCGGTAATCCTGCTCGACCTTTACCCAAGGCAGGGCGCGACGGGCTGCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAac                                                                                                                               <  2:4479880/150‑1 (MQ=255)
                           aTTTGGCCCGAAGAAGCGGTAATCCTGCTCGACCTTTACCCAAGGCAGGGCGCGACGGGCTGCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAaccac                                                                                                                            >  2:354778/1‑150 (MQ=255)
                              tGGCCCGAAGAAGCGGTAATCCTGCTCGACCTTTACCCAAGGCAGGGCGCGACGGGCTGCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCgg                                                                                                                         <  1:2413600/150‑1 (MQ=255)
                                gCCCGAAGAAGCGGTAATCCTGCTCGACCTTTACCCAAGGCAGGGCGCGACGGGCTGCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGt                                                                                                                       >  2:1420253/1‑150 (MQ=255)
                                  ccGAAGAAGCGGTAATCCTGCTCGACCTTTACCCAAGGCAGGGCGCGACGGGCTGCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGc                                                                                                                     >  1:3388091/1‑150 (MQ=255)
                                    gaagaaGCGGTAATCCTGCTCGACCTTTACCCAAGGCAGGGCGCGACGGGCTGCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCTGGTGCCt                                                                                                                   <  2:4067407/150‑1 (MQ=255)
                                     aagaagCGGTAATCCTGCTCGACCTTTACCCAAGGCAGGGCGCGACGGGCTGCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTg                                                                                                                  >  1:1930669/1‑150 (MQ=255)
                                     aagaagCGGTAATCCTGCTCGACCTTTACCCAAGGCAGGGCGCGACGGGCTGCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTg                                                                                                                  >  1:2556299/1‑150 (MQ=255)
                                         agCGGTAATCCTGCTCTAACTTTAACCAAGGCAGGGCGCGACGGGCTGCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCttt                                                                                                              <  1:2677644/150‑1 (MQ=255)
                                          gCGGTAATCCTGCTCGACCTTTACCCAAGGCAGGGCGCGACGGGCTGCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTc                                                                                                             <  1:1127603/150‑1 (MQ=255)
                                            ggTAATCCTGCTCGACCTTTACCCAAGGCAGGGCGCGACGGGCTGCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTcgc                                                                                                           <  1:507555/150‑1 (MQ=255)
                                            ggTAATCCTGCTCGACCTTTACCCAAGGCAGGGCGCGACGGGCTGCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACACTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTcgc                                                                                                           <  2:4251362/150‑1 (MQ=255)
                                            ggTAAGCCGGCTCGACCTTGCCCCCCGGCAGGGCGCGACGGGCTGCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTcgc                                                                                                           <  1:1652612/150‑1 (MQ=255)
                                              tAATCCTGCTCGACCTTTACCCAAGGCAGGGCGCGACGGGCTGCAGTTCGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCgt                                                                                                         <  2:3141565/150‑1 (MQ=255)
                                               aaTCCTGCTCGACCTTTACCCAAGGCAGGGCGCGACGGGCTGCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCgtg                                                                                                        >  1:4398170/1‑150 (MQ=255)
                                                 tCCTGCTCGACCTTTACCCAAGGCAGGGCGCGACGGGCTGCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGtgtg                                                                                                      <  2:4367143/150‑1 (MQ=255)
                                                 tCCTGCTCGACCTTTACCCAAGGCAGGGCGCGACGGGCTGCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGtgtg                                                                                                      >  1:2072849/1‑150 (MQ=255)
                                                  ccTGCTCGACCTTTACCCAAGGCAGGGCGCGACGGGCTGCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGc                                                                                                     >  2:2400622/1‑150 (MQ=255)
                                                  ccTGCTCGACCTTTACCCAAGGCAGGGCGCGACGGGCTGCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGc                                                                                                     >  1:3785140/1‑150 (MQ=255)
                                                  ccTGCTCGACCTTTACCCAAGGCAGGGCGCGACGGGCTGCAGTGAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGc                                                                                                     <  2:2152575/150‑1 (MQ=255)
                                                       tCGACCTTTACCCAAGGCAGGGCGCGACGGGCTGCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATg                                                                                                <  1:570121/150‑1 (MQ=255)
                                                       tCGACCTTTACCCAAGGCAGGGCGCGACGGGCTGCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATg                                                                                                <  1:715644/150‑1 (MQ=255)
                                                                 cccAAGGCAGGGCGCGACGGGCTGCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGtctc                                                                                      <  1:90659/150‑1 (MQ=255)
                                                                  ccAAGGCAGGGCGCGACGGGCTGCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTctcc                                                                                     >  2:2789201/1‑150 (MQ=255)
                                                                  ccAAGGCAGGGCGCGACGGGCTGCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTctcc                                                                                     <  2:3282534/150‑1 (MQ=255)
                                                                  ccAAGGCAGGGCGCGACGGGCTGCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTctcc                                                                                     >  1:4422895/1‑150 (MQ=255)
                                                                    aaGGCAGGGCGCGACGGGCTGCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTctcctc                                                                                   >  1:1754882/1‑150 (MQ=255)
                                                                    aaGGCAGGGCGCGACGGGCTGCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTctcctc                                                                                   >  1:3064227/1‑150 (MQ=255)
                                                                       gCAGGGCGCGACGGGCTGCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGTAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCt                                                                                >  1:1266306/1‑150 (MQ=255)
                                                                       gCAGGGCGCGACGGGCTGCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCt                                                                                >  1:4158423/1‑150 (MQ=255)
                                                                         aGGGCGCGACGGGCTGCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTgg                                                                              <  2:2153459/150‑1 (MQ=255)
                                                                          gggCGCGACGGGCTGCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTggg                                                                             <  2:1207832/150‑1 (MQ=255)
                                                                           ggCGCGACGGGCTGCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTgggg                                                                            >  2:4393119/1‑150 (MQ=255)
                                                                                gACGGGCTGCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTc                                                                       >  1:2504328/1‑150 (MQ=255)
                                                                                 aCGGGCTGCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTca                                                                      <  2:2017346/150‑1 (MQ=255)
                                                                                     gCTGCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTCACAcc                                                                  >  1:2671608/1‑150 (MQ=255)
                                                                                     gCTGCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTCACAcc                                                                  <  2:1204436/150‑1 (MQ=255)
                                                                                       tGCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTCACACCtt                                                                <  1:2088910/150‑1 (MQ=255)
                                                                                        gCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCTTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTCACACCTTg                                                               <  1:2845505/150‑1 (MQ=255)
                                                                                          aGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTCACACCTTGGt                                                             <  2:1209763/150‑1 (MQ=255)
                                                                                            ttAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTCACACCTTGGTCg                                                           >  2:3755982/1‑150 (MQ=255)
                                                                                             tAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTCACACCTTGGTCGa                                                          <  2:129417/150‑1 (MQ=255)
                                                                                             tAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTCACACCTTGGTCGa                                                          <  1:4078003/150‑1 (MQ=255)
                                                                                               gcgcGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTCACACCTTGGTCGAGt                                                        >  2:3788357/1‑150 (MQ=255)
                                                                                                cgcgTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTCACACCTTGGTCGAGTa                                                       <  2:748176/150‑1 (MQ=255)
                                                                                                 gcgTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTCACACCTTGGTCGAGTAc                                                      >  2:3782965/1‑150 (MQ=255)
                                                                                                     cgcgGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTCACACCTTGGTCGAGTACGAcc                                                  >  1:3478541/1‑150 (MQ=255)
                                                                                                            gtggGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTCACACCTTGGTCGAGTACGACCAGGTCGa                                           >  2:3632647/1‑150 (MQ=255)
                                                                                                             tggGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTCACACCTTGGTCGAGTACGACCAGGTCGAt                                          >  1:3318740/1‑150 (MQ=255)
                                                                                                              gggTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTCACACCTTGGTCGAGTACGACCAGGTCGAtt                                         >  1:1792379/1‑150 (MQ=255)
                                                                                                              gggTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTACTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTCACACCTTGGTCGAGTACGACCAGGTCGAtt                                         <  2:1870528/150‑1 (MQ=255)
                                                                                                              gggTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTACTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTCACACCTTGGTCGAGTACGACCAGGTCGAtt                                         <  2:1870913/150‑1 (MQ=255)
                                                                                                               ggTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTCACACCTTGGTCGAGTACGACCAGGTCGATTc                                        >  2:1793189/1‑150 (MQ=255)
                                                                                                                   aaCGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTCACACCTTGGTCGAGTACGACCAGGTCGATTCGACa                                    >  1:1172873/1‑150 (MQ=255)
                                                                                                                    aCGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTCACACCTTGGTCGAGTACGACCAGGTCGATTCGACaa                                   <  2:1799091/150‑1 (MQ=255)
                                                                                                                    aCGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTCACACCTTGGTCGAGTACGACCAGGTCGATTCGACaa                                   <  2:1524038/150‑1 (MQ=255)
                                                                                                                     cGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGTCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTCACACCTTGGTCGAGTACGACCAGGTCGATTCGACAAg                                  >  1:355547/1‑150 (MQ=255)
                                                                                                                     cGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTCACACCTTGGTCGAGTACGACCAGGTCGATTCGACAAg                                  >  1:3887665/1‑150 (MQ=255)
                                                                                                                        tttttCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTCACACCTTGGTCGAGTACGACCAGGTCGATTCGACAAGCAc                               >  2:3412496/1‑150 (MQ=255)
                                                                                                                        tttttCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTCACACCTTGGTCGAGTACGACCAGGTCGATTCGACAAGCAc                               >  1:3583496/1‑150 (MQ=255)
                                                                                                                               tGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTCACACCTTGGTCGAGTACGACCAGGTCGATTCGACAAGCACCGGTGGc                        >  1:1527314/1‑150 (MQ=255)
                                                                                                                               tGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTCACACCTTGGTCGAGTACGACCAGGTCGATTCGACAAGCACCGGTGGc                        >  2:1862204/1‑150 (MQ=255)
                                                                                                                                gCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTCACACCTTGGTCGAGTACGACCAGGTCGATTCGACAAGCACCGGTGGcc                       <  2:4156051/150‑1 (MQ=255)
                                                                                                                                gCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTCACACCTTGGTCGAGTACGACCAGGTCGATTCGACAAGCACCGGTGGcc                       >  2:1761993/1‑150 (MQ=255)
                                                                                                                                   gCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTCACACCTTGGTCGAGTACGACCAGGTCGATTCGACAAGCACCGGTGGCCggg                    >  2:3385043/1‑150 (MQ=255)
                                                                                                                                       caGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTCACGCCTTGGTCGAGTACGACCAGGTCGATTCGACAAGCACCGGTGGCCGGGCGAt                <  2:4195027/150‑1 (MQ=255)
                                                                                                                                       caGTGGCTGGCGGCTCGCCAGCAAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTCACACCTTGGTCGAGTACGACCAGGTCGATTCGACAAGCACCGGTGGCCGGGCGAt                >  1:4005377/1‑150 (MQ=255)
                                                                                                                                         gtggCTGGCGGCTCGCCAGCAACTGTGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTCACACCTTGGTCGAGTACGACCAGGTCGATTCGACAAGCACCGGTGGCCGGGCGAt                >  2:2557130/4‑150 (MQ=255)
                                                                                                                                         gTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTCACACCTTGGTCGAGTACGACCAGGTCGATTCGACAAGCACCGGTGGCCGGGCGATGa              >  2:1033893/1‑150 (MQ=255)
                                                                                                                                         gTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTCACACCTTGGTCGAGTACGACCAGGTCGATTCGACAAGCACCGGTGGCCGGGCGATGa              <  2:3847562/150‑1 (MQ=255)
                                                                                                                                          ttGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTCACACCTTGGTCGAGTACGACCAGGTCGATTCGACAAGCACCGGTGGCCGGGCGATGAg             <  1:3632647/150‑1 (MQ=255)
                                                                                                                                          ttGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTCACACCTTGGTCGAGTACGACCAGGTCGATTCGACAAGCACCGGTGGCCGGGCGATGAg             <  1:4479221/150‑1 (MQ=255)
                                                                                                                                           tGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTCACACCTTGGTCGAGTACGACCAGGTCGATTCGACAAGCACCGGTGGCCGGGCGATGAGc            <  1:1671826/150‑1 (MQ=255)
                                                                                                                                              tGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTCACACCTTGGTCGAGTACGACCAGGTCGATTCGACAAGCACCGGTGGCCGGGCGATGAGCGGt         >  1:3635514/1‑150 (MQ=255)
                                                                                                                                                gCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTCACACCTTGGTCGAGTACGACCAGGTCGATTCGACAAGCACCGGTGGCCGGGCGATGAGCGGTAt       <  2:804419/150‑1 (MQ=255)
                                                                                                                                                gCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTCACACCTTGGTCGAGTACGACCAGGTCGATTCGACAAGCACCGGTGGCCGGGCGATGAGCGGTAt       >  1:324963/1‑150 (MQ=255)
                                                                                                                                                gCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTCACACCTTGGTCGAGTACGACCAGGTCGATTCGACAAGCACCGGTGGCCGGGCGATGAGCGGTAt       >  2:3120377/1‑150 (MQ=255)
                                                                                                                                                 cGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTCACACCTTGGTCGAGTACGACCAGGTCGATTCGACAAGCACCGGTGGCCGGGCGATGAGCGGTATc      <  2:2960892/150‑1 (MQ=255)
                                                                                                                                                 cGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTCACACCTTGGTCGAGTACGACCAGGTCGATTCGACAAGCACCGGTGGCCGGGCGATGAGCGGTATc      <  1:1862204/150‑1 (MQ=255)
                                                                                                                                                 cGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTCACACCTTGGTCGAGTACGACCAGGTCGATTCGACAAGCACCGGTGGCCGGGCGATGAGCGGTATc      <  1:1609923/150‑1 (MQ=255)
                                                                                                                                                 cGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTCACACCTTGGTCGAGTACGACCAGGTCGATTCGACAAGCACCGGTGGCCGGGCGATGAGCGGTATc      <  2:2258071/150‑1 (MQ=255)
                                                                                                                                                  gAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTCACACCTTGGTCGAGTACGACCAGGTCGATTCGACAAGCACCGGTGGCCGGGCGATGAGCGGTATCt     >  1:3218530/1‑150 (MQ=255)
                                                                                                                                                    gccgccAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTCACACCTTGGTCGAGTACGACCAGGTCGATTCGACAAGCACCGGTGGCCGGGCGATGAGCGGTATCTGc   <  2:1256305/150‑1 (MQ=255)
                                                                                                                                                     ccgccAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTCACACCTTGGTCGAGTACGACCAGGTCGATTCGACAAGCACCGGTGGCCGGGCGATGAGCGGTATCTGCt  >  2:4170607/1‑150 (MQ=255)
                                                                                                                                                     ccgccAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTCACACCTTGGTCGAGTACGACCAGGTCGATTCGACAAGCACCGGTGGCCGGGCGATGAGCGGTATCTGCt  <  2:2815423/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                       
GAACAGGTCGGCCAGACTCAACGCGCCATTTGGCCCGAAGAAGCGGTAATCCTGCTCGACCTTTACCCAAGGCAGGGCGCGACGGGCTGCAGTTAGCGCGTCGCGGTGGTGGGTAAACGCTTTTTCGTGCAGCCACAGTTGCTGGCGAGCCGCCAGCCAC‑‑TGGCTGCGGGAAACCACCGGGTGCCTGCTTTCGCGTGTGCTCATGGTGCTGTCTCCTCGCTGGGGGATTCACACCTTGGTCGAGTACGACCAGGTCGATTCGACAAGCACCGGTGGCCGGGCGATGAGCGGTATCTGCT  >  NC_002947/3255203‑3255501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑