BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,438,160	T→A	7.2%	W44R (TGG→AGG)	gpI →	tail formation protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,438,160	0	T	A	7.2%	754.0 / 35.3	235	W44R (TGG→AGG)	gpI	tail formation protein
Reads supporting (aligned to +/- strand): ref base T (103/114); new base A (7/10); total (110/125)
Fisher's exact test for biased strand distribution p-value = 8.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

Only 100 of 249 total aligned reads displayed.

CTGACGTCGAAGGTGGTGACATGAGCCTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGTGCTCAAGGCGGTTCGGGTC  >  NC_002947/3438011‑3438306

 tGACGTCGAAGGTGGTGACATGAGCCTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCctg                                                                                                                                                   >  1:1998156/1‑150 (MQ=255)

   aCGTCGAAGGTGGTGACATGAGCCTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGATATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCctggc                                                                                                                                                 >  1:2203155/1‑150 (MQ=255)

        gAAGGTGGTGACATGAGCCTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGcc                                                                                                                                            <  1:2661696/150‑1 (MQ=255)

         aaGGTGGTGACATGAGCCTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCt                                                                                                                                           >  2:3657245/1‑150 (MQ=255)

          aGGTGGTGACATGAGCCTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTg                                                                                                                                          <  2:3768745/150‑1 (MQ=255)

           ggtggtGACATGAGCCTGTTACCGCACCACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACCTTAGCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTgg                                                                                                                                         <  2:3054350/150‑1 (MQ=255)

           ggtggtGACATGAGCCTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTgg                                                                                                                                         <  1:4489536/150‑1 (MQ=255)

             tggtgACATGAGCCTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGc                                                                                                                                       >  2:4410080/1‑150 (MQ=255)

               gtgACATGAGCCTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCAt                                                                                                                                     <  1:2514476/150‑1 (MQ=255)

               gtgACATGAGCCTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCAt                                                                                                                                     <  1:2105269/150‑1 (MQ=255)

                  aCATGAGCCTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCAGGCCAGCCAGGGCaggaa                                                                                                                                  <  2:4497642/150‑5 (MQ=255)

                   cATGAGCCTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGaa                                                                                                                                 >  2:4117998/1‑150 (MQ=255)

                   cATGAGCCTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGaa                                                                                                                                 <  1:863872/150‑1 (MQ=255)

                      gAGCCTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGt                                                                                                                              >  2:3158669/1‑150 (MQ=255)

                       aGCCTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGACCTTCCATGCCCAGGCCAGCCAGGGCA‑GGAAGTTc                                                                                                                             <  2:1565606/150‑1 (MQ=38)

                         ccTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGa                                                                                                                           <  1:998337/150‑1 (MQ=255)

                          cTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCC‑TCGACCTTCCATGCCCAGGCCAGCCAGGGCA‑GGAAGTTCggcg                                                                                                                         <  2:2375588/150‑4 (MQ=25)

                           tGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAgg                                                                                                                         >  1:3699340/1‑150 (MQ=255)

                             ttACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGc                                                                                                                       >  2:3935394/1‑150 (MQ=255)

                                ccGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTgg                                                                                                                    >  2:3231558/1‑150 (MQ=255)

                                ccGCACAACGCCACCCAGGTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCGGGCTGGCCTGGGCATGGAAGGTCGAGGGCTgg                                                                                                                    <  1:2029448/150‑1 (MQ=255)

                                  gCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGa                                                                                                                  <  1:3486319/150‑1 (MQ=255)

                                   cacaACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATgg                                                                                                                                   >  2:145381/1‑132 (MQ=255)

                                     caACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAggc                                                                                                               >  2:1466587/1‑150 (MQ=255)

                                     caACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAggc                                                                                                               <  1:1179400/150‑1 (MQ=255)

                                      aaCGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAggcg                                                                                                              >  1:1623259/1‑150 (MQ=255)

                                        cGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAggcggc                                                                                                            <  2:4004738/150‑1 (MQ=255)

                                        cGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAggcggc                                                                                                            <  1:3875499/150‑1 (MQ=255)

                                           cACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTa                                                                                                         >  1:661/1‑150 (MQ=255)

                                            aCCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTac                                                                                                        >  2:2769962/1‑150 (MQ=255)

                                            aCCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTac                                                                                                        <  1:4325725/150‑1 (MQ=255)

                                            aCCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTac                                                                                                        <  2:623090/150‑1 (MQ=255)

                                                       gcgcgcCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGa                                                                                                                  <  1:3735913/129‑1 (MQ=255)

                                                        cgcgcCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAg                                                                                            <  2:1770538/150‑1 (MQ=255)

                                                          cgcCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAgcg                                                                                          <  1:2600661/150‑1 (MQ=255)

                                                            cccTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGcgcc                                                                                        <  2:2600577/150‑1 (MQ=255)

                                                              cTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGACGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGGCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGcgccgc                                                                                      >  2:67464/1‑150 (MQ=255)

                                                                 gAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCcgcgcg                                                                                   >  1:4346204/1‑150 (MQ=255)

                                                                  cggcggcCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCgcgcgc                                                                                  <  1:312888/149‑1 (MQ=255)

                                                                    gcggcCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTTGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTg                                                                                >  2:4191746/1‑150 (MQ=255)

                                                                    gcggcCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTg                                                                                >  1:1700309/1‑150 (MQ=255)

                                                                      ggcCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGCCTC‑‑‑‑C‑CAGCCC‑TCGACCTTCC‑TGCCCTGGCTGGCCTgg                                                                                                                                         <  2:1025136/85‑1 (MQ=16)

                                                                      ggcCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGAt                                                                              >  1:565692/1‑150 (MQ=255)

                                                                       gccgccGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATc                                                                             >  2:1603176/1‑150 (MQ=255)

                                                                          gccgACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCcgc                                                                          >  2:1560795/1‑150 (MQ=255)

                                                                            cgACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGa                                                                        >  1:360235/1‑150 (MQ=255)

                                                                                 ttGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCa                                                                   >  1:150134/1‑150 (MQ=255)

                                                                                 ttGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCa                                                                   >  2:3462710/1‑150 (MQ=255)

                                                                                     ccTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTcc                                                               >  1:132929/1‑150 (MQ=255)

                                                                                      cTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCg                                                              >  1:1902274/1‑150 (MQ=255)

                                                                                      cTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCg                                                              >  2:4253627/1‑150 (MQ=255)

                                                                                        tGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGt                                                            >  2:2005316/1‑150 (MQ=255)

                                                                                        tGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGt                                                            >  2:2005093/1‑150 (MQ=255)

                                                                                         gACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGtt                                                           <  2:1289537/150‑1 (MQ=255)

                                                                                          aCCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGc                                                                                                                                       <  1:2867755/73‑1 (MQ=255)

                                                                                          aCCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGc                                                                                                                                       >  2:2867755/1‑73 (MQ=255)

                                                                                           cccGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTca                                                         >  1:4489420/1‑150 (MQ=255)

                                                                                           cccGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTca                                                         <  1:1469900/150‑1 (MQ=255)

                                                                                            ccGGACATTATCCGGGGCGTGGCCGACTGGTCACGCTGCCCGCCGAACTACC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTcac                                                        >  1:337596/1‑150 (MQ=255)

                                                                                            ccGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGACGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTcac                                                        >  2:2725671/1‑150 (MQ=255)

                                                                                            ccGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTcac                                                        >  1:2263532/1‑150 (MQ=255)

                                                                                              ggACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACaa                                                      <  1:720678/150‑1 (MQ=255)

                                                                                              ggACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACaa                                                      <  1:2548326/150‑1 (MQ=255)

                                                                                              ggACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACaa                                                      >  2:1335980/1‑150 (MQ=255)

                                                                                              ggACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACaa                                                      <  1:1691542/150‑1 (MQ=255)

                                                                                                  aTTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGAcc                                                  >  1:2970170/1‑150 (MQ=255)

                                                                                                   ttATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCa                                                 >  1:104854/1‑150 (MQ=255)

                                                                                                      tCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAgg                                              <  1:4264834/150‑1 (MQ=255)

                                                                                                       ccGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCCTTCCGGTTCACAAGACCAAggg                                             >  1:4170052/1‑150 (MQ=255)

                                                                                                       ccGGGGCGCGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAggg                                             <  2:1397355/150‑1 (MQ=255)

                                                                                                              gTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTc                                      <  2:1498262/150‑1 (MQ=255)

                                                                                                                  ccGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGgcg                                  <  1:2180839/150‑1 (MQ=255)

                                                                                                                  ccGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGgcg                                  <  1:334478/150‑1 (MQ=255)

                                                                                                                   cGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGgcgc                                 <  1:1793971/150‑1 (MQ=255)

                                                                                                                      cTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGAt                              <  1:100802/150‑1 (MQ=255)

                                                                                                                       tCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGAtt                             <  1:4268230/150‑1 (MQ=255)

                                                                                                                          tCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGTGGGCGGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTcgg                          >  2:2323458/1‑150 (MQ=255)

                                                                                                                          tCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTcgg                          >  1:2211731/1‑150 (MQ=255)

                                                                                                                            aCGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTcggcg                        >  1:3243326/1‑150 (MQ=255)

                                                                                                                             cGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTcggcgg                       <  1:3810868/150‑1 (MQ=255)

                                                                                                                               cTGCCCGCCGAACGTCC‑TGCCCTGGCTGGCCTGGGCAAGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGt                     <  2:3380588/150‑1 (MQ=255)

                                                                                                                                cagccctcgACCTTCCATGCCCAGGCCAGCCAGGGCA‑GGAAGTTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCATGGTCGGCGCGATTCGGCGGGTg                    >  2:862106/8‑150 (MQ=25)

                                                                                                                                 gCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGTGc                   >  1:4119418/1‑150 (MQ=255)

                                                                                                                                   ccgccgAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGACATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGTGCTc                 <  2:3328129/150‑1 (MQ=255)

                                                                                                                                    cgccgAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCAGTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGTGCTCa                >  2:4086678/1‑150 (MQ=255)

                                                                                                                                           cTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGTGCTCAAGGCGGt         <  1:956770/150‑1 (MQ=255)

                                                                                                                                            ttCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGTGCTCAAGGCGGtt        >  1:1321095/1‑150 (MQ=255)

                                                                                                                                             tccatGCCCAGGCCAGCCAGGGCA‑GGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGTGCTCAAGGCGGTTc       >  2:1808037/5‑150 (MQ=255)

                                                                                                                                             tCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGTGCTCAAGGCGGTTc       <  2:2522551/150‑1 (MQ=255)

                                                                                                                                              cc‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGTGCTCAAGGCGGTTCg      >  2:2827576/1‑150 (MQ=255)

                                                                                                                                               c‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGTGCTCAAGGCGGTTCgg     <  2:3842313/150‑1 (MQ=255)

                                                                                                                                               c‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGTGCTCAAGGCGGTTCgg     <  1:2133860/150‑1 (MQ=255)

                                                                                                                                               c‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGTGCTCAAGGCGGTTCgg     <  2:2203350/150‑1 (MQ=255)

                                                                                                                                               c‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGTGCTCAAGGCGGTTCgg     <  2:2203155/150‑1 (MQ=255)

                                                                                                                                               c‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGTGCTCAAGGCGGTTCgg     <  2:3565771/150‑1 (MQ=255)

                                                                                                                                                atGCCCAGGCCAGCCAGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGTGCTCAAGGCGGTTCgg     >  2:797518/2‑150 (MQ=255)

                                                                                                                                                 tGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGTGCTCAAGGCGGTTCggg    <  1:2747885/150‑1 (MQ=255)

                                                                                                                                                 tGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGTGCTCAAGGCGGTTCggg    >  2:1651575/1‑150 (MQ=255)

                                                                                                                                                 tGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGTGCTCAAGGCGGTTCggg    >  1:1828703/1‑150 (MQ=255)

                                                                                                                                                 tGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGTGCTCAAGGCGGTTCggg    <  1:3426796/150‑1 (MQ=255)

CTGACGTCGAAGGTGGTGACATGAGCCTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGTGCTCAAGGCGGTTCGGGTC  >  NC_002947/3438011‑3438306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`