BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,438,164	T→C	7.2%	L45P (CTG→CCG) ‡	gpI →	tail formation protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,438,164	0	T	C	7.2%	736.1 / 30.8	235	L45P (CTG→CCG) ‡	gpI	tail formation protein
Reads supporting (aligned to +/- strand): ref base T (103/114); new base C (7/10); total (110/125)
Fisher's exact test for biased strand distribution p-value = 8.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.58e-01

Only 100 of 239 total aligned reads displayed.

GTCGAAGGTGGTGACATGAGCCTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGTGCTCAAGGCGGTTCGGGTCAACGCGG  >  NC_002947/3438016‑3438313

gTCGAAGGTGGTGACATGAGCCTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCctggctg                                                                                                                                                      <  2:432536/150‑1 (MQ=255)

 tCGAAGGTGGTGACATGAGCCTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCctggctgg                                                                                                                                                     <  1:4281792/150‑1 (MQ=255)

    aaGGTGGTGACATGAGCCTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCt                                                                                                                                                  >  2:3657245/1‑150 (MQ=255)

      ggtggtGACATGAGCCTGTTACCGCACCACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACCTTAGCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTgg                                                                                                                                                <  2:3054350/150‑1 (MQ=255)

       gtggtgACATGAGCCTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCAGGCCAGCCAggg                                                                                                                                               <  2:3888609/150‑1 (MQ=255)

        tggtgACATGAGCCTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGc                                                                                                                                              >  2:4410080/1‑150 (MQ=255)

         ggtgACATGAGCCTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCa                                                                                                                                             >  2:3810868/1‑150 (MQ=255)

         ggtgACATGAGCCTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCAGGCCAGCCAGGGCa                                                                                                                                             <  1:3652225/150‑1 (MQ=255)

          gtgACATGAGCCTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCAt                                                                                                                                            <  1:2105277/150‑1 (MQ=255)

           tgACATGAGCCTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATg                                                                                                                                           >  1:2522551/1‑150 (MQ=255)

             aCATGAGCCTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGa                                                                                                                                         >  2:1431150/1‑150 (MQ=255)

              cATGAGCCTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGaa                                                                                                                                        <  1:863872/150‑1 (MQ=255)

                 gAGCCTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGt                                                                                                                                     >  2:3158669/1‑150 (MQ=255)

                  aGCCTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGACCTTCCATGCCCAGGCCAGCCAGGGCA‑GGAAGTTc                                                                                                                                    <  2:1564981/150‑1 (MQ=38)

                  aGCCTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGACCTTCCATGCCCAGGCCAGCCAGGGCA‑GGAAGTTc                                                                                                                                    <  1:2686756/150‑1 (MQ=38)

                  aGCCTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTc                                                                                                                                    <  1:2155677/150‑1 (MQ=255)

                  aGCCTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCGTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGACCTTCCATGCCCAGGCCAGCCAGGGCA‑GGAAGTTc                                                                                                                                    <  2:1565636/150‑1 (MQ=38)

                     cTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAg                                                                                                                                 <  1:376956/150‑1 (MQ=255)

                     cTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCC‑TCGACCTTCCATGCCCAGGCCAGCCAGGGCA‑GGAAGTTCggcg                                                                                                                                <  2:2375588/150‑4 (MQ=25)

                       ggtACCGCCCACCGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTGGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAggg                                                                                                                               <  2:1341002/148‑1 (MQ=255)

                           ccGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTgg                                                                                                                           >  2:3231558/1‑150 (MQ=255)

                            cGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTggg                                                                                                                          >  1:1920487/1‑150 (MQ=255)

                              cacaACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATgg                                                                                                                                          <  1:145381/132‑1 (MQ=255)

                                caACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAggc                                                                                                                      >  2:1466602/1‑150 (MQ=255)

                                caACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAggc                                                                                                                      >  2:1466573/1‑150 (MQ=255)

                                caACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAggc                                                                                                                      <  1:1179400/150‑1 (MQ=255)

                                 aaCGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAggcg                                                                                                                     >  1:1623248/1‑150 (MQ=255)

                                   cGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAggcggc                                                                                                                   <  1:3875499/150‑1 (MQ=255)

                                   cGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAggcggc                                                                                                                   <  1:4412673/150‑1 (MQ=255)

                                      cACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTa                                                                                                                >  1:661/1‑150 (MQ=255)

                                       aCCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTac                                                                                                               <  2:623090/150‑1 (MQ=255)

                                       aCCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTac                                                                                                               >  2:2769962/1‑150 (MQ=255)

                                       aCCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTACCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGTTGGGAGGCGGCTTac                                                                                                               <  1:1784931/150‑1 (MQ=255)

                                         ccAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTacac                                                                                                             >  1:2191434/1‑150 (MQ=255)

                                          cAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACAcc                                                                                                            <  2:3367152/150‑1 (MQ=255)

                                                gAGCGCGCCGTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCgacgac                                                                                                      <  1:972512/150‑1 (MQ=255)

                                                  gcgcgcCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGa                                                                                                                         >  2:3735913/1‑129 (MQ=255)

                                                  gcgcgcCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGa                                                                                                                         <  1:3735913/129‑1 (MQ=255)

                                                   cgcgcCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAg                                                                                                   <  2:1770538/150‑1 (MQ=255)

                                                     cgcCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAgcg                                                                                                 <  1:2600661/150‑1 (MQ=255)

                                                       cccTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGcgcc                                                                                               <  2:2600577/150‑1 (MQ=255)

                                                         cTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGACGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGGCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGcgccgc                                                                                             >  2:67464/1‑150 (MQ=255)

                                                            gAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCcgcgcg                                                                                          >  1:4346204/1‑150 (MQ=255)

                                                             cggcggcCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCgcgcgc                                                                                         <  1:312888/149‑1 (MQ=255)

                                                               gcggcCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTTGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTg                                                                                       >  2:4191746/1‑150 (MQ=255)

                                                                 ggcCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGCCTC‑‑‑‑C‑CAGCCC‑TCGACCTTCC‑TGCCCTGGCTGGCCTgg                                                                                                                                                <  2:1025136/85‑1 (MQ=16)

                                                                 ggcCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGAt                                                                                     >  1:565321/1‑150 (MQ=255)

                                                                     gccgACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCcgc                                                                                 >  2:1560793/1‑150 (MQ=255)

                                                                     gccgACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCcgc                                                                                 <  2:1632228/150‑1 (MQ=255)

                                                                       cgACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGa                                                                               >  1:360235/1‑150 (MQ=255)

                                                                       cgACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGa                                                                               <  1:1825977/150‑1 (MQ=255)

                                                                        gACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAg                                                                              <  1:2014517/150‑1 (MQ=255)

                                                                            ttGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCa                                                                          >  2:3462710/1‑150 (MQ=255)

                                                                            ttGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCa                                                                          >  1:150134/1‑150 (MQ=255)

                                                                               gCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTc                                                                       >  2:4093269/1‑150 (MQ=255)

                                                                                ccTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTcc                                                                      >  1:132929/1‑150 (MQ=255)

                                                                                   tGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGt                                                                   >  2:2005093/1‑150 (MQ=255)

                                                                                    gACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGtt                                                                  <  2:4157890/150‑1 (MQ=255)

                                                                                      cccGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTca                                                                >  1:4489456/1‑150 (MQ=255)

                                                                                       ccGGACATTATCCGGGGCGTGGCCGACTGGTCACGCTGCCCGCCGAACTACC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTcac                                                               >  1:337596/1‑150 (MQ=255)

                                                                                         ggACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACaa                                                             >  2:1335980/1‑150 (MQ=255)

                                                                                         ggACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACaa                                                             <  1:720678/150‑1 (MQ=255)

                                                                                         ggACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACaa                                                             <  1:1691542/150‑1 (MQ=255)

                                                                                           ataatgtCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGa                                                           >  1:348590/7‑150 (MQ=255)

                                                                                             aTTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGAcc                                                         >  1:2970170/1‑150 (MQ=255)

                                                                                              ttATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCa                                                        >  1:104854/1‑150 (MQ=255)

                                                                                                  ccGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCCTTCCGGTTCACAAGACCAAggg                                                    >  1:4170052/1‑150 (MQ=255)

                                                                                                    ggggCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCa                                                  >  2:1039195/1‑150 (MQ=255)

                                                                                                             ccGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGgcg                                         <  1:334478/150‑1 (MQ=255)

                                                                                                                 cTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGAt                                     <  1:100802/150‑1 (MQ=255)

                                                                                                                  tCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGAtt                                    <  1:4268230/150‑1 (MQ=255)

                                                                                                                     tCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGTGGGCGGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTcgg                                 >  2:2323458/1‑150 (MQ=255)

                                                                                                                     tCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTcgg                                 >  2:2135066/1‑150 (MQ=255)

                                                                                                                      cACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTcggc                                >  2:1846693/1‑150 (MQ=255)

                                                                                                                       aCGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTcggcg                               >  1:3243326/1‑150 (MQ=255)

                                                                                                                         gCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCCTTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCggg                             >  2:2908303/1‑150 (MQ=255)

                                                                                                                          cTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGt                            <  2:3560376/150‑1 (MQ=255)

                                                                                                                           cagccctcgACCTTCCATGCCCAGGCCAGCCAGGGCA‑GGAAGTTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCATGGTCGGCGCGATTCGGCGGGTg                           >  2:862106/8‑150 (MQ=25)

                                                                                                                            gCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGTGc                          <  2:521841/150‑1 (MQ=255)

                                                                                                                            gCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGTGc                          >  1:4119418/1‑150 (MQ=255)

                                                                                                                              ccgccgAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGTGCTc                        <  1:2878846/150‑1 (MQ=255)

                                                                                                                              ccgccgAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGACATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGTGCTc                        <  2:3328129/150‑1 (MQ=255)

                                                                                                                                gccgAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGTGCTCaa                      <  1:2223064/150‑1 (MQ=255)

                                                                                                                                gccgAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGTGCTCaa                      <  1:2094131/150‑1 (MQ=255)

                                                                                                                                ctcgACCTTCC‑TGCCCTGGCTGGCCTGGGCCTGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCCTTCCGGTTCACAAGACCAAGGGCCCGGTCGGCGCGAGTCGGCGGGTGCTCaa                      >  2:1692558/3‑150 (MQ=255)

                                                                                                                                     aCTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGTGCTCAAGGCgg                 <  1:2572002/150‑1 (MQ=255)

                                                                                                                                       ttCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGTGCTCAAGGCGGtt               >  2:3978529/1‑150 (MQ=255)

                                                                                                                                       ttCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGTGCTCAAGGCGGtt               >  1:1321095/1‑150 (MQ=255)

                                                                                                                                        tCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGTGCTCAAGGCGGTTc              <  2:2522551/150‑1 (MQ=255)

                                                                                                                                         cc‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGTGCTCAAGGCGGTTCg             >  2:2827576/1‑150 (MQ=255)

                                                                                                                                          c‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGTGCTCAAGGCGGTTCgg            <  2:2203155/150‑1 (MQ=255)

                                                                                                                                          c‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGTGCTCAAGGCGGTTCgg            <  2:2203350/150‑1 (MQ=255)

                                                                                                                                          c‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGTGCTCAAGGCGGTTCgg            <  1:1692965/150‑1 (MQ=255)

                                                                                                                                           atGCCCAGGCCAGCCAGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGTGCTCAAGGCGGTTCgg            >  2:2690688/2‑150 (MQ=255)

                                                                                                                                           atGCCCAGGCCAGCCAGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGTGCTCAAGGCGGTTCgg            >  2:797518/2‑150 (MQ=255)

                                                                                                                                            tGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGTGCTCAAGGCGGTTCggg           <  1:3426796/150‑1 (MQ=255)

                                                                                                                                            tGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGTGCTCAAGGCGGTTCggg           >  1:1828703/1‑150 (MQ=255)

                                                                                                                                            tGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGTGCTCAAGGCGGTTCggg           <  1:2747885/150‑1 (MQ=255)

                                                                                                                                             gCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGTGCTCAAGGCGGTTCGGGt          >  1:4219642/1‑150 (MQ=255)

                                                                                                                                              cccTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGTGCTCAAGGCGGTTCGGGTc         >  1:240291/1‑150 (MQ=255)

GTCGAAGGTGGTGACATGAGCCTGTTACCGCACAACGCCACCCAGTTGGAGCGCGCCCTGGAGGCGGCCGCCGACCTTGGCCTTGACCCGGACATTATCCGGGGCGTGGCCGACTCGTCACGCTGCCCGCCGAACTTCC‑TGCCCTGGCTGGCCTGGGCATGGAAGGTCGAGGGCTGGGAGGCGGCTTACACCGACGACCAGCGCCGCGCGCTGATCCGCGAGGCCATTCCGGTTCACAAGACCAAGGGCACGGTCGGCGCGATTCGGCGGGTGCTCAAGGCGGTTCGGGTCAACGCGG  >  NC_002947/3438016‑3438313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`