Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,827,929 C→A 100% V28F (GTC→TTC)  ptxS ← 2‑ketogluconate utilization repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,827,9290CA100.0% 784.2 / NA 212V28F (GTC→TTC) ptxS2‑ketogluconate utilization repressor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (84/128);  total (84/128)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 212 total aligned reads displayed.

CCAGCATGCCGATCAGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGTTTCAATACAGCAAAAATGCGCGGG  >  NC_002947/3827782‑3828074
                                                                                                                                                   |                                                                                                                                                 
ccaGCATGCCGATCAGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAgg                                                                                                                                                 >  1:452596/1‑150 (MQ=255)
 caGCATGCCGATCAGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGt                                                                                                                                                <  1:1421518/150‑1 (MQ=255)
 caGCATGCCGATCAGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGt                                                                                                                                                <  2:3513392/150‑1 (MQ=255)
 caGCATGCCGATCAGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCGTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGt                                                                                                                                                >  1:367380/1‑150 (MQ=255)
  aGCATGCCGATCAGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTg                                                                                                                                               <  1:720565/150‑1 (MQ=255)
       gCCGATCAGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCttt                                                                                                                                          <  2:294011/150‑1 (MQ=255)
       gCCGATCAGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCttt                                                                                                                                          <  1:1365846/150‑1 (MQ=255)
        ccGATCAGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTg                                                                                                                                         <  2:3755034/150‑1 (MQ=255)
         cGATCAGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTgg                                                                                                                                        >  2:763085/1‑150 (MQ=255)
          gATCAGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGa                                                                                                                                       >  1:850065/1‑150 (MQ=255)
          gATCAGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGa                                                                                                                                       <  1:1535899/150‑1 (MQ=255)
           aTCAGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGaa                                                                                                                                      >  2:3364882/1‑150 (MQ=255)
               gTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGc                                                                                                                                  <  1:506177/150‑1 (MQ=255)
               gTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGc                                                                                                                                  <  1:2761230/150‑1 (MQ=255)
                tCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGcc                                                                                                                                 >  2:2449870/1‑150 (MQ=255)
                          ccGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACAcgc                                                                                                                       <  2:4278461/150‑1 (MQ=255)
                          ccGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACAcgc                                                                                                                       <  2:1481981/150‑1 (MQ=255)
                            gcgcTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACgcgc                                                                                                                     <  2:4013814/150‑1 (MQ=255)
                            gcgcTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACgcgc                                                                                                                     >  2:3905086/1‑150 (MQ=255)
                            gcgcTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACgcgc                                                                                                                     <  1:3551116/150‑1 (MQ=255)
                                   aGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCa                                                                                                              <  1:4298968/150‑1 (MQ=255)
                                     gCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACt                                                                                                            >  2:3930990/1‑150 (MQ=255)
                                     gCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACt                                                                                                            >  1:1573571/1‑150 (MQ=255)
                                                tCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGAccc                                                                                                 <  1:468726/150‑1 (MQ=255)
                                                  tGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGt                                                                                               >  1:2672639/1‑150 (MQ=255)
                                                  tGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGt                                                                                               >  2:1069598/1‑150 (MQ=255)
                                                          gCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGt                                                                                       >  1:1004090/1‑150 (MQ=255)
                                                            gATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTAt                                                                                     >  2:1620402/1‑150 (MQ=255)
                                                             aTAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGAAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATg                                                                                    <  2:1832867/150‑1 (MQ=255)
                                                                  cGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAg                                                                               >  2:3670811/1‑150 (MQ=255)
                                                                  cGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAg                                                                               <  1:2562082/150‑1 (MQ=255)
                                                                  cGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAg                                                                               <  1:2027112/150‑1 (MQ=255)
                                                                        gTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTGTGGACGCGCCAGCACCACGCGCCAGGGGGCTGATGGGGACCCGGTCGCGGGTAGGGGCGGGcgcgg                                                                         >  2:4120692/1‑149 (MQ=255)
                                                                         ttCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTc                                                                        >  2:2311319/1‑150 (MQ=255)
                                                                         ttCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTc                                                                        <  2:1143387/150‑1 (MQ=255)
                                                                         ttCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTc                                                                        <  2:3628426/150‑1 (MQ=255)
                                                                         ttCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTc                                                                        >  2:503804/1‑150 (MQ=255)
                                                                          tCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCg                                                                       <  1:3351407/150‑1 (MQ=255)
                                                                            gATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGt                                                                     >  1:3830623/1‑150 (MQ=255)
                                                                            gATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGt                                                                     >  2:319531/1‑150 (MQ=255)
                                                                            gATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGt                                                                     <  1:1335342/150‑1 (MQ=255)
                                                                             aTGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTc                                                                    >  2:354350/1‑150 (MQ=255)
                                                                               gACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCAt                                                                  >  1:318162/1‑150 (MQ=255)
                                                                                 ccTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATgg                                                                <  1:2855575/150‑1 (MQ=255)
                                                                                  ctctTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATggg                                                               >  1:3225986/1‑150 (MQ=255)
                                                                                  ctctTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATggg                                                               >  1:1620468/1‑150 (MQ=255)
                                                                                  ctctTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATggg                                                               >  2:4040851/1‑150 (MQ=255)
                                                                                       ccAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTc                                                          >  1:1504661/1‑150 (MQ=255)
                                                                                        cAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTcg                                                         >  2:3695194/1‑149 (MQ=255)
                                                                                        cAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTcc                                                         <  1:7582/150‑1 (MQ=255)
                                                                                        cAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTcc                                                         <  2:3846685/150‑1 (MQ=255)
                                                                                          gCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCtt                                                       >  1:1811488/1‑150 (MQ=255)
                                                                                          gCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCtt                                                       >  1:3599399/1‑150 (MQ=255)
                                                                                           ccGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTa                                                      <  1:1242558/150‑1 (MQ=255)
                                                                                           ccGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTa                                                      <  1:3888665/150‑1 (MQ=255)
                                                                                           ccGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTa                                                      <  1:3921248/150‑1 (MQ=255)
                                                                                           ccGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTa                                                      <  1:1242640/150‑1 (MQ=255)
                                                                                             gCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATg                                                    >  2:4513712/1‑150 (MQ=255)
                                                                                              cTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGa                                                   <  2:2588155/150‑1 (MQ=255)
                                                                                                   ccGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTAGGATTGtt                                              >  1:4143874/1‑150 (MQ=255)
                                                                                                       ggCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTaa                                          >  2:3655905/1‑150 (MQ=255)
                                                                                                       ggCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCAGGGGTTTTCCTTATGATTGTTTTaa                                          >  2:3373505/1‑150 (MQ=255)
                                                                                                       agccacGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTaa                                          >  1:4395710/6‑150 (MQ=255)
                                                                                                        gCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAAt                                         <  1:2367639/150‑1 (MQ=255)
                                                                                                          ttCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAg                                       <  1:1896474/150‑1 (MQ=255)
                                                                                                          ttCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAg                                       >  1:876595/1‑150 (MQ=255)
                                                                                                          ttCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAg                                       >  1:2891884/1‑150 (MQ=255)
                                                                                                           tCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGt                                      <  1:374351/150‑1 (MQ=255)
                                                                                                             ggCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGtga                                    <  2:3965028/150‑1 (MQ=255)
                                                                                                             ggCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGtga                                    >  2:4063002/1‑150 (MQ=255)
                                                                                                              gCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGtgat                                   <  2:847787/150‑1 (MQ=255)
                                                                                                              gCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGtgat                                   <  1:1393507/150‑1 (MQ=255)
                                                                                                              gCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGtgat                                   <  2:2730549/150‑1 (MQ=255)
                                                                                                              gCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGtgat                                   <  1:1391487/150‑1 (MQ=255)
                                                                                                              gCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGtgat                                   <  2:4335090/150‑1 (MQ=255)
                                                                                                              gCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGtgat                                   >  2:2253905/1‑150 (MQ=255)
                                                                                                              gCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGtgat                                   <  1:1264997/150‑1 (MQ=255)
                                                                                                              gCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGtgat                                   >  2:1734680/1‑150 (MQ=255)
                                                                                                              gCCAGCAATTGGCGGTCGCCACCGAGGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGtgat                                   <  1:2518393/150‑1 (MQ=255)
                                                                                                               ccAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGtgatg                                  >  2:2748066/1‑150 (MQ=255)
                                                                                                               ccAGCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGtgatg                                  >  2:1983691/1‑150 (MQ=255)
                                                                                                                  gCAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGaaa                               >  1:353993/1‑150 (MQ=255)
                                                                                                                   cAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAAc                              >  2:1162509/1‑150 (MQ=255)
                                                                                                                   cAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAAc                              <  2:4435994/150‑1 (MQ=255)
                                                                                                                   cAATTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAAc                              <  2:2662379/150‑1 (MQ=255)
                                                                                                                     aTTGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCg                            <  2:164668/150‑1 (MQ=255)
                                                                                                                      ttGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCgg                           <  2:2055016/150‑1 (MQ=255)
                                                                                                                       tGGCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGt                          <  2:617416/150‑1 (MQ=255)
                                                                                                                         gCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGttt                        <  1:2311319/150‑1 (MQ=255)
                                                                                                                         gCGGTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGttt                        <  1:3113879/150‑1 (MQ=255)
                                                                                                                            gTCGCCACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGTTTCaa                     <  1:3845428/150‑1 (MQ=255)
                                                                                                                              cGCCTCCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGTTTCAATa                   <  1:1519457/150‑1 (MQ=255)
                                                                                                                                 cACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGTTTCAATACAg                >  2:546652/1‑150 (MQ=255)
                                                                                                                                 cACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGTTTCAATACAg                >  1:2133381/1‑150 (MQ=255)
                                                                                                                                 cACCGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGTTTCAATACAg                <  2:1342531/150‑1 (MQ=255)
                                                                                                                                    cGATGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGTTTCAATACAGCaa             >  2:1814256/1‑150 (MQ=255)
                                                                                                                                      aTGTAACGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGTTTCAATACAGCaaaa           >  1:4016173/1‑150 (MQ=255)
                                                                                                                                           aCGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGTTTCAATACAGCAAAAATgcg      >  2:3433333/1‑150 (MQ=255)
                                                                                                                                           aCGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGTTTCAATACAGCAAAAATgcg      >  1:3544063/1‑150 (MQ=255)
                                                                                                                                            cGGGAGAAGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGTTTCAATACAGCAAAAATgcgc     <  1:1761339/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CCAGCATGCCGATCAGTCGCGTCTGGCCGCGCTTTAGGCCGCGAGCCATCTGGTTGGGGCGATAGCCGAGGCGTTCGATGACCTCTTCCAGCCGCTTGGCCGTGGCTTCGGCCAGCAATTGGCGGTCGCCACCGATGTAACGGGAGACGGTGGCTTTGGAAACGCCAGCAACACGCGCCACTTCACTGATGGTGACCCGTTCGCGGGTATGGGCAGGCGCGTCGGTCATGGGTTTTCCTTATGATTGTTTTAATAGTGATGAAACCGGTTTCAATACAGCAAAAATGCGCGGG  >  NC_002947/3827782‑3828074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑