Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,865,806 A→G 6.0% P921P (CCA→CCG) ‡ PP_3413 → two‑component system sensor histidine kinase/response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,865,8060AG 6.0% 701.8 / 21.5 216P921P (CCA→CCG) ‡PP_3413two‑component system sensor histidine kinase/response regulator
Reads supporting (aligned to +/- strand):  ref base A (84/119);  new base G (9/4);  total (93/123)
Fisher's exact test for biased strand distribution p-value = 7.97e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.70e-01

Only 100 of 230 total aligned reads displayed.

GTGACACTGGCGGAGAATGGCGAGATCGCCCTTGCACGCTGGCAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTGTTCAAGCCGATTGGTCTGCGCACGCTCAAGACCCACCTGCCCCAGGTGCGCCCTCAGCAGCCGTC  >  NC_002947/3865657‑3865955
                                                                                                                                                     |                                                                                                                                                     
gtgACACTGGCGGAGAATGGCGAGATCGCCCTTGCACGCTGGCAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCca                                                                                                                                                       <  2:4152108/150‑1 (MQ=255)
  gACACTGGCGGAGAATGGCGAGATCGCCCTTGCACGCTGGCAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCcagc                                                                                                                                                     >  1:3003548/1‑150 (MQ=255)
   acacTGGCGGAGAATGGCGAGATCGCCCTTGCACGCTGGCAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCcagca                                                                                                                                                    >  2:195051/1‑150 (MQ=255)
   acacTGGCGGAGAATGGCGAGATCGCCCTTGCACGCTGGCAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCcagca                                                                                                                                                    >  2:194965/1‑150 (MQ=255)
   acacTGGCGGAGAATGGCGAGATCGCCCTTGCACGCTGGCAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCcagca                                                                                                                                                    <  2:809446/150‑1 (MQ=255)
     acTGGCGGAGAATGGCGAGATCGCCCTTGCACGCTGGCAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAgg                                                                                                                                                  >  1:1886970/1‑150 (MQ=255)
      cTGGCGGAGAATGGCGAGATCGCCCTTGCACGCTGGCAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGc                                                                                                                                                 <  1:3852209/150‑1 (MQ=255)
       tGGCGGAGAATGGCGAGATCGCCCTTGCACGCTGGCAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGcc                                                                                                                                                >  1:3792384/1‑150 (MQ=255)
               aaTGGCGAGATCGCCCTTGCACGCTGGCAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCtt                                                                                                                                        <  1:574660/150‑1 (MQ=255)
                aTGGCGAGATCGCCCTTGCACGCTGGCAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTg                                                                                                                                       <  2:2315321/150‑1 (MQ=255)
                  ggCGAGATCGCCCTTTCACGCTGGCAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGc                                                                                                                                     <  2:639791/150‑1 (MQ=255)
                  ggCGAGATCGCCCTTGCACGCTGGCAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGc                                                                                                                                     <  2:273226/150‑1 (MQ=255)
                  ggCGAGATCGCCCTTGCACGCTGGCAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGc                                                                                                                                     <  1:4442873/150‑1 (MQ=255)
                  ggCGAGATCGCCCTTGCACGCTGGCAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGc                                                                                                                                     <  1:53344/150‑1 (MQ=255)
                  ggCGAGATCGCCCTTGCACGCTGGCAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGc                                                                                                                                     <  1:1753943/150‑1 (MQ=255)
                  ggCGAGATCGCCCTTGCACGCTGGCAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGc                                                                                                                                     <  2:2418837/150‑1 (MQ=255)
                  ggCGAGATCGCCCTTGCACGCTGGCAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGc                                                                                                                                     <  2:4499992/150‑1 (MQ=255)
                    cGAGATCGCCCTTGCACGCTGGCAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGt                                                                                                                                   >  2:115537/1‑150 (MQ=255)
                        aTCGCCCTTGCACGCTGGCAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAATTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGAcc                                                                                                                               <  1:978460/150‑1 (MQ=255)
                        aTCGCCCTTGCACGCTGGCAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGAcc                                                                                                                               >  2:1277689/1‑150 (MQ=255)
                          cGCCCTTGCACGCTGGCAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGc                                                                                                                             <  2:911313/150‑1 (MQ=255)
                            cccTTGCACGCTGGCAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCa                                                                                                                           >  1:1414548/1‑150 (MQ=255)
                             ccTTGCACGCTGGCAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTAGGCGTGACCGCCaa                                                                                                                          >  1:3494918/1‑150 (MQ=255)
                              cTTGCACGCTGGCAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAAt                                                                                                                         <  1:1991861/150‑1 (MQ=255)
                               ttGCACGCTGGCAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATg                                                                                                                        >  1:903313/1‑150 (MQ=255)
                               ttGCACGCTGGCAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATg                                                                                                                        >  1:1389363/1‑150 (MQ=255)
                                 gCACGCTGGCAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGcc                                                                                                                      <  1:1817213/150‑1 (MQ=255)
                                 gCACGCTGGCAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCAGTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGcc                                                                                                                      >  2:3620968/1‑150 (MQ=255)
                                         gCAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCga                                                                                                              >  2:1518048/1‑150 (MQ=255)
                                          cAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCgag                                                                                                             <  2:387198/150‑1 (MQ=255)
                                           aGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTGTTCTTGGCGTGACCGCCAATGCCCAGGcagcga                                                                                                            <  2:1884539/150‑6 (MQ=255)
                                           aGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCgagg                                                                                                            >  2:4118556/1‑150 (MQ=255)
                                           aGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCgagg                                                                                                            >  1:3410346/1‑150 (MQ=255)
                                            ggAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCgagga                                                                                                           <  2:3373883/150‑1 (MQ=255)
                                             gAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCgaggag                                                                                                          <  2:571451/150‑1 (MQ=255)
                                             gAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCgaggag                                                                                                          <  2:2111477/150‑1 (MQ=255)
                                              aaGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGc                                                                                                         <  2:735192/150‑1 (MQ=255)
                                              aaGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGc                                                                                                         <  2:4086069/150‑1 (MQ=255)
                                              aaGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGc                                                                                                         <  1:315092/150‑1 (MQ=255)
                                              aaGCGCGCTTCGACCTGGTGATCACCGAGTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGc                                                                                                         <  2:2990796/150‑1 (MQ=255)
                                                 cgcgcTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTa                                                                                                      >  2:2836865/1‑150 (MQ=255)
                                                  gcgcTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTAc                                                                                                     >  2:2000513/1‑150 (MQ=255)
                                                  gcgcTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTAc                                                                                                     >  2:3281101/1‑150 (MQ=255)
                                                  gcgcTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTAc                                                                                                     >  2:4269485/1‑150 (MQ=255)
                                                        cGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTg                                                                                               <  1:2808261/150‑1 (MQ=255)
                                                        cGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTg                                                                                               <  1:27468/150‑1 (MQ=255)
                                                         gACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGc                                                                                              <  1:3135482/150‑1 (MQ=255)
                                                           ccTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCt                                                                                            <  1:2127694/150‑1 (MQ=255)
                                                             tGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTgg                                                                                          >  1:2585215/1‑150 (MQ=255)
                                                              ggTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGc                                                                                         <  1:3893439/150‑1 (MQ=255)
                                                               gTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTgg                                                                                                                                      <  1:4145048/104‑1 (MQ=255)
                                                                     aCCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGc                                                                                  >  1:789772/1‑150 (MQ=255)
                                                                     aCCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGc                                                                                  >  1:477182/1‑150 (MQ=255)
                                                                           tGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGAc                                                                            <  1:2514879/150‑1 (MQ=255)
                                                                                 aTGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGcc                                                                                                                <  2:2632134/108‑1 (MQ=255)
                                                                                   gCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCt                                                                    <  1:4012239/150‑1 (MQ=255)
                                                                                   gCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCt                                                                    <  1:3575005/150‑1 (MQ=255)
                                                                                   gCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCt                                                                    <  2:3255232/150‑1 (MQ=255)
                                                                                   gCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCt                                                                    <  1:4519425/150‑1 (MQ=255)
                                                                                         cATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTGTTCaa                                                              <  2:614625/150‑1 (MQ=255)
                                                                                          aTGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTGTTCAAg                                                             <  1:3174007/150‑1 (MQ=255)
                                                                                           tGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTGTTCAAGc                                                            <  2:1717330/150‑1 (MQ=255)
                                                                                           tGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTGTTCAAGc                                                            <  2:1597991/150‑1 (MQ=255)
                                                                                            ggACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTGTTCAAGcc                                                           >  2:212171/1‑150 (MQ=255)
                                                                                            ggACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTGTTCAAGcc                                                           >  2:2278857/1‑150 (MQ=255)
                                                                                               cGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTGTTCAAGCCGAt                                                        <  1:3927366/150‑1 (MQ=255)
                                                                                                  gCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTGTTCAAGCCGATTgg                                                     >  2:3906161/1‑150 (MQ=255)
                                                                                                   cATGAACTCACCCGGCGCATTCGCAGCCTGGAGAGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTGTTCAAGCCGATTGGt                                                    <  2:3752228/150‑1 (MQ=255)
                                                                                                    aTGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTGTTCAAGCCGATTGGTc                                                   >  1:3652623/1‑150 (MQ=255)
                                                                                                          tCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTGTTCAAGCCGATTGGTCTGCGCa                                             >  1:115381/1‑150 (MQ=255)
                                                                                                          tCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTGTTCAAGCCGATTGGTCTGCGCa                                             >  1:3058389/1‑150 (MQ=255)
                                                                                                          tCACCCGGCGCATTCGAAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTGTTCAAGCCGATTGGTCTGCGCa                                             >  2:1183553/1‑150 (MQ=255)
                                                                                                           cACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTGTTCAAGCCGATTGGTCTGCGCAc                                            <  2:4356948/150‑1 (MQ=255)
                                                                                                            aCCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTGTTCAAGCCGATTGGTCTGCGCACg                                           >  2:3229266/1‑150 (MQ=255)
                                                                                                             cccGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTGTTCAAGCCGATTGGTCTGCGCACGc                                          <  1:4196652/150‑1 (MQ=255)
                                                                                                              ccGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTGTTCAAGCCGATTGGTCTGCGCACGCt                                         >  1:4296898/1‑150 (MQ=255)
                                                                                                                ggCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTGTTCAAGCCGATTGGTCTGCGCACGCTCa                                       <  1:1243152/150‑1 (MQ=255)
                                                                                                                  cgcATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTGTTCAAGCCGATTGGTCTGCGCACGCTCAAg                                     <  1:699626/150‑1 (MQ=255)
                                                                                                                   gcATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTGTTCAAGCCGATTGGTCTGCGCACGCTCAAGa                                    <  1:2898539/150‑1 (MQ=255)
                                                                                                                   gcATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTGTTCAAGCCGATTGGTCTGCGCACGCTCAAGa                                    <  2:4414647/150‑1 (MQ=255)
                                                                                                                     aTTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTGTTCAAGCCGATTGGTCTGCGCACGCTCAAGAcc                                  >  1:2646512/1‑150 (MQ=255)
                                                                                                                     aTTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGACCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTGTTCAAGCCGATTGGTCTGCGCACGCTCAAGAcc                                  >  1:2274257/1‑150 (MQ=255)
                                                                                                                        cGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTGTTCAAGCCGATTGGTCTGCGCACGCTCAAGACCCAc                               <  1:1629229/150‑1 (MQ=255)
                                                                                                                         gCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGACTGCCTGTTCAAGCCGATTGGTCTGCGCACGCTCAAGACCCAcc                              >  2:3383620/1‑150 (MQ=255)
                                                                                                                         gCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGACCAGCGGCATGGACGAATGCCTGTTCAAGCCGATTGGTCTGCGCACGCTCAAGACCCAcc                              >  2:3588430/1‑150 (MQ=255)
                                                                                                                             ccTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTGTTCAAGCCGATTGGTCTGCGCACGCTCAAGACCCACCTGcc                          >  1:3633388/1‑150 (MQ=255)
                                                                                                                                   gggCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCTGCATGGACGAATGCCTGTTCAAGCCGATTGGTCTGCGCACGCTCAAGTCCCACCTGCCCCAGGt                    <  2:3649881/150‑1 (MQ=255)
                                                                                                                                     aagaatacgGCCTGCTGGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTGTTCAAGCCGATTGGTCTGCGCACGCTCAAGACCCACCTGCCCCAGGTgc                  >  1:1407964/8‑150 (MQ=255)
                                                                                                                                       gAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTGTTCAAGCCGATTGGTCTGCGCACGCTCAAGACCCACCTGCCCCAGGTgcgc                <  1:2686068/150‑1 (MQ=255)
                                                                                                                                       gAGCGCGGCCGGCCAGCTGGCCGTATTCTTGGCGTGAGCGGCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTGTTCAAGCCGATTGGTCTGCGCACGCTCAAGACCCACCTGCCCCAGGTgcgc                <  2:369349/150‑1 (MQ=255)
                                                                                                                                         atacgGCCTGCTGGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTGTTCAAGCCGATTGGTCTGCGCGCGCTCAAGACCCACCTGCCCCAGGTGCGccc              >  2:3090877/4‑150 (MQ=255)
                                                                                                                                         atacgGCCTGCTGGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTGTTCAAGCCGATTGGTCTGCGCACGCTCAAGACCCACCTGCCCCAGGTGCGccc              >  1:225170/4‑150 (MQ=255)
                                                                                                                                          tacgGCCTGCTGGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTGTTCAAGCCGATTGGTCTgc                                                >  2:824922/3‑115 (MQ=255)
                                                                                                                                          cgcgGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTGTTCAAGCCGATTGGTCTGCGCACGCTCAAGACCCACCTGCCCCAGGTGCGCCCt             <  1:2544686/150‑1 (MQ=255)
                                                                                                                                            cgGCCTGCTGGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTGTTCAAGCCGATTGGTCTGCGCACGCTCAAGACCCACCTGCCCCAGGTGCGCCCTca           >  2:2924086/1‑150 (MQ=255)
                                                                                                                                            cgGCCTGCTGGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTGTTCAAGCCGATTGGTCTGCGCACGCTCAAGACCCACCTGCCCCAGGTGCGCCCTca           >  2:192689/1‑150 (MQ=255)
                                                                                                                                                cTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTGTTCAAGCCGATTGGTCTGCGCACGCTCAAGACCCACCTGCCCCAGGTGCGCCCTcagcag       >  2:1020429/1‑150 (MQ=255)
                                                                                                                                                     agcagGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTGTTCAAGCCGATTGGTCTGCGCACGCTCAAGACCCACCTGCCCCAGGTGCGCCCTCAGCAGCCGTc  >  2:2207133/1‑150 (MQ=255)
                                                                                                                                                     agcagGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTGTTCAAGCCGATTGGTCTGCGCACGCTCAAGACCCACCTGCCCCAGGTGCGCCCTCAGCAGCCGTc  >  1:1518746/1‑150 (MQ=255)
                                                                                                                                                     agcagGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTGTTCAAGCCGATTGGTCTGCGCACGCTCAAGACCCACCTGCCCCAGGTGCGCCCTCAGCAGCCGTc  >  2:1377843/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTGACACTGGCGGAGAATGGCGAGATCGCCCTTGCACGCTGGCAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTGTTCAAGCCGATTGGTCTGCGCACGCTCAAGACCCACCTGCCCCAGGTGCGCCCTCAGCAGCCGTC  >  NC_002947/3865657‑3865955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑