BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,951,063	G→A	6.1%	P146L (CCG→CTG)	PP_3485 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,951,063	0	G	A	6.1%	577.5 / 18.3	181	P146L (CCG→CTG)	PP_3485	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (79/90); new base A (2/9); total (81/100)
Fisher's exact test for biased strand distribution p-value = 1.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

Only 100 of 196 total aligned reads displayed.

GCGCCTGCGCCTCCCGCTGCCCGATGAGCGTTGCCCGAGCCAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTGCGCCCTGTCGAAGCCCGCCTCTGCCAGTCGCCTTTCGAATAACGCCGGGCTGCCAAATGCCGCTTCGATCTCGCC  >  NC_002947/3950914‑3951211

gcgcctgcgcctCCCGCTGCCCGATGAGCGTTGCCCGAGCCAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCctcgctcg                                                                                                                                                        >  1:3305508/1‑150 (MQ=255)

  gcctgcgcctCCCGCTGCCCGATGAGCGTTGCCCGAGCCAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGgc                                                                                                                                                      <  1:1598223/150‑1 (MQ=255)

    ctgcgcctCCCGCTGCCCGATGAGCGTTGCCCGAGCCAAGGCCAGGCCGTGTTCGCGTATGCCCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGgcgc                                                                                                                                                    <  2:3447643/150‑1 (MQ=255)

    ctgcgcctCCCGCTGCCCGATGAGCGTTGCCCGAGCCAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGgcgc                                                                                                                                                    >  2:139447/1‑150 (MQ=255)

      gcgcctCCCGCTGCCCGATGAGCGTTGCCCGAGCCAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑Gt                                                                                                                                                 >  1:1198652/1‑150 (MQ=255)

         cctCCCGCTGCCCGATGAGCGTTGCCCGAGCCAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGa                                                                                                                                              <  1:579420/150‑1 (MQ=255)

           tCCCGCTGCCCGATGAGCGTTGCCCGAGCCAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACt                                                                                                                                            <  2:3836708/150‑1 (MQ=255)

           tCCCGCTGCCCGATGAGCGTTGCCCGAGCCAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACt                                                                                                                                            >  2:4124503/1‑150 (MQ=255)

              cGCTGCCCGATGAGCGTTGCCCGAGCCAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTgcg                                                                                                                                         >  1:2931127/1‑150 (MQ=255)

                  gCCCGATGAGCGTTGCCCGAGCCAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGa                                                                                                                                     <  2:1201531/150‑1 (MQ=255)

                  gCCCGATGAGCGTTGCCCGAGCCAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGa                                                                                                                                     <  1:305059/150‑1 (MQ=255)

                     cGATGAGCGTTGCCCGAGCCAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCT‑GCTCAGCGCAGTCGAC‑GCGCCGAGcga                                                                                                                                 >  2:2463667/1‑148 (MQ=255)

                      gATGAGCGTTGCCCGAGCCAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑Ag                                                                                                                                <  1:3287903/150‑1 (MQ=255)

                      gATGAGCGTTGCCCGAGCCAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCAGCGCAGTCGAC‑GCGCCGAGcga                                                                                                                                 <  2:667941/150‑3 (MQ=255)

                       aTGAGCGTTGCCCGAGCCAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑Agg                                                                                                                               <  2:3191408/150‑1 (MQ=255)

                        tGAGCGTTGCCCGAGCCAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCT‑GCTCAGCGCAGTCGAC‑GCGCCGAGcgaggg                                                                                                                              <  1:501203/150‑6 (MQ=38)

                        tGAGCGTTGCCCGAGCCAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCT‑GCTCAGCGCAGTCGAC‑GCGCCGAGcgaggg                                                                                                                              <  1:4037251/150‑6 (MQ=38)

                         gAGCGTTGCCCGAGCCAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGc                                                                                                                             <  2:2638299/150‑1 (MQ=255)

                            cGTTGCCCGAGCCAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTa                                                                                                                          <  2:1291411/150‑1 (MQ=255)

                              ttGCCCGAGCCAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTaca                                                                                                                        <  1:2929789/150‑1 (MQ=255)

                                 cccGAGCCAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCT‑GCTCAGCGCAGTCGAC‑GCGCCGAGCGAGGGCgaagtgga                                                                                                                     <  1:2463667/150‑8 (MQ=37)

                                  ccGAGCCAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTg                                                                                                                    >  1:2057113/1‑150 (MQ=255)

                                  ccGAGCCAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTg                                                                                                                    >  1:2200733/1‑150 (MQ=255)

                                  ccGAGCCAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCCTGCTCAGCGCAGTCGAC‑GCGCCGAGCGAGGGCgaagtgga                                                                                                                     <  2:527871/150‑8 (MQ=37)

                                     aGCCAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTg                                                                                                                 <  1:721069/150‑1 (MQ=255)

                                      gCCAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTgg                                                                                                                <  2:3230644/150‑1 (MQ=255)

                                        cAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGgc                                                                                                              <  2:1858695/150‑1 (MQ=255)

                                         acggccaggccGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCCGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGgcc                                                                                                             <  1:272298/148‑1 (MQ=255)

                                         aaGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGgcc                                                                                                             >  1:4230782/1‑150 (MQ=255)

                                         aaGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGgcc                                                                                                             >  2:3029111/1‑150 (MQ=255)

                                            gccaggccGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGgccgcc                                                                                                          <  1:3015644/150‑1 (MQ=255)

                                            gccaggccGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGgccgcc                                                                                                          <  2:3860276/150‑1 (MQ=255)

                                            gccaggccGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGgccgcc                                                                                                          <  1:1230797/150‑1 (MQ=255)

                                              caggccGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCAt                                                                                                        >  2:2845922/1‑150 (MQ=255)

                                                        tCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCgg                                                                                              <  1:1488292/150‑1 (MQ=255)

                                                        tCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCgg                                                                                              <  1:1779355/150‑1 (MQ=255)

                                                        tCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCgg                                                                                              <  1:1374163/150‑1 (MQ=255)

                                                        tCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCgg                                                                                              <  2:2057113/150‑1 (MQ=255)

                                                          gcgTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGgt                                                                                            <  1:2609598/150‑1 (MQ=255)

                                                                  ccAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAg                                                                                    <  1:2287141/150‑1 (MQ=255)

                                                                  ccAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAg                                                                                    <  1:3377168/150‑1 (MQ=255)

                                                                   cAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAgt                                                                                   >  1:3561463/1‑150 (MQ=255)

                                                                   cAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAgt                                                                                   >  2:3722166/1‑150 (MQ=255)

                                                                   cAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAgt                                                                                   >  2:466982/1‑150 (MQ=255)

                                                                    aaTGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAgtg                                                                                  <  1:3817260/150‑1 (MQ=255)

                                                                    aaTGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAgtg                                                                                  >  1:3792521/1‑150 (MQ=255)

                                                                    aaTGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAgtg                                                                                  >  1:1357876/1‑150 (MQ=255)

                                                                    aaTGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAgtg                                                                                  <  1:179801/150‑1 (MQ=255)

                                                                      tGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGgt                                                                                            <  2:3570341/138‑1 (MQ=255)

                                                                      tGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTa                                                                                >  1:3621602/1‑150 (MQ=255)

                                                                         aTTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTg                                                                             <  1:2802551/150‑1 (MQ=255)

                                                                           tATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTgcg                                                                           >  2:1457855/1‑150 (MQ=255)

                                                                             tCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTGCGcc                                                                         >  1:3923492/1‑150 (MQ=255)

                                                                             tCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTGCGcc                                                                         >  1:3629614/1‑150 (MQ=255)

                                                                                 tttGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTGCGCCCTGt                                                                     >  1:2696277/1‑150 (MQ=255)

                                                                                 tttGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTGCGCCCTGt                                                                     >  2:4061768/1‑150 (MQ=255)

                                                                                 tttGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTGCGCCCTGt                                                                     >  2:4061850/1‑150 (MQ=255)

                                                                                  ttGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCTGGTGTATTCAGTGTACTGCGCCCTGTc                                                                    <  2:1717192/150‑1 (MQ=255)

                                                                                     gTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCACAAAATGCCTCCACTCCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTGCGCCCTGTCGaa                                                                 <  2:2351242/150‑1 (MQ=255)

                                                                                     gTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTGCGCCCTGTCGaa                                                                 <  2:3105170/150‑1 (MQ=255)

                                                                                     gTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTGCGCCCTGTCGaa                                                                 <  2:1680182/150‑1 (MQ=255)

                                                                                     gTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTGCGCCCTGTCGaa                                                                 <  1:4400273/150‑1 (MQ=255)

                                                                                     gTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTGCGCCCTGTCGaa                                                                 <  1:2874236/150‑1 (MQ=255)

                                                                                      ttcttcGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCTCTTCGCCCTCGCTCGGCGC‑TTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTGCGCCCTGTCTAAg                                                                <  1:375360/150‑1 (MQ=255)

                                                                                      ttCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTGCGCCCTGTCGAAg                                                                >  2:719058/1‑150 (MQ=255)

                                                                                        cTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTGCGCCCTGTCGAAGcc                                                              <  2:513641/150‑1 (MQ=255)

                                                                                         tGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTGCGCCCTGTCGAAGccc                                                             <  2:1240886/150‑1 (MQ=255)

                                                                                               ccTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTGCGCCCTGTCGAAGCCCGCctct                                                       <  1:3418801/150‑1 (MQ=255)

                                                                                               ccTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTGCGCCCTGTCGAAGCCCGCctct                                                       <  1:2255512/150‑1 (MQ=255)

                                                                                                 ttGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTGCGCCCTGTCGAAGCCCGCCTCTGc                                                     >  2:2764965/1‑150 (MQ=255)

                                                                                                   gCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTGCGCCCTGTCGAAGCCCGCCTCTGCCa                                                   <  1:3854794/150‑1 (MQ=255)

                                                                                                      gTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTGCGCCCTGTCGAAGCCCGCCTCTGCCAGTc                                                <  1:2737314/150‑1 (MQ=255)

                                                                                                         tGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTGCGCCCTGTCGAAGCCCGCCTCCGCCAGTCGcc                                             <  1:1648762/150‑1 (MQ=255)

                                                                                                           tCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTGCGCCCTGTCGAAGCCCGCCTCTGCCAGTCGCCtt                                           >  2:2295441/1‑150 (MQ=255)

                                                                                                            ccTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTGCGCCCTGTCGAAGCCCGCCTCTGCCAGTCGCCttt                                          >  1:1124402/1‑150 (MQ=255)

                                                                                                             cTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTGCGCCCTGTCGAAGCCCGCCTCTGCCAGTCGCCTTTc                                         <  2:3389186/150‑1 (MQ=255)

                                                                                                                gTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTGCGCCCTGTCGAAGCCCGCCTCTGCCAGTCGCCTTTCGaa                                      <  1:1076022/150‑1 (MQ=255)

                                                                                                                      aTCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTGCGCCCTGTCGAAGCCCGCCTCTGCCAGTCGCCTTTCGAATAACGc                                <  2:3305508/150‑1 (MQ=255)

                                                                                                                      aTCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTGCGCCCTGTCGAAGCCCGCCTCTGCCAGTCGCCTTTCGAATAACGc                                >  2:777643/1‑150 (MQ=255)

                                                                                                                       tCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTGCGCCCTGTCGAAGCCCGCCTCTGCCAGTCGCCtt                                           >  2:2254128/1‑138 (MQ=255)

                                                                                                                       tCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTGCGCCCTGTCGAAGCCCGCCTCTGCCAGTCGCCTTTCGAATAACGcc                               >  1:4117290/1‑150 (MQ=255)

                                                                                                                       tCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTGCGCCCTGTCGAAGCCCGCCTCTGCCAGTCGCCTTTCGAATAACGcc                               >  1:107865/1‑150 (MQ=255)

                                                                                                                       tCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTGCGCCCTGTCGAAGCCCGCCTCTGCCAGTCGCCTTTCGAATAACGcc                               >  1:2320236/1‑150 (MQ=255)

                                                                                                                       tCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTGCGCCCTGTCGAAGCCCGCCTCTGCCAGTCGCCTTTCGAATAACGcc                               >  1:855412/1‑150 (MQ=255)

                                                                                                                        cAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTGCGCCCTGTCGAAGCCCGCCTCTGCCAGTCGCCTTTCGAATAACGCCg                              >  2:1115072/1‑150 (MQ=255)

                                                                                                                        cAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTGCGCCCTGTCGAAGCCCGCCTCTGCCAGTCGCCTTTCGAATAACGCCg                              >  2:624929/1‑150 (MQ=255)

                                                                                                                          aaaaaTGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTGCGCCCTGTCGAAGCCCGCCTCTGCCAGTCGCCTTTCGAATAACGCCggg                            >  1:1118841/1‑150 (MQ=255)

                                                                                                                            aaaTGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTGCGCCCTGTCGAAGCCCGCCTCTGCCAGTCGCCTTTCGAATAACGCCGGGCt                          <  2:2190060/150‑1 (MQ=255)

                                                                                                                                   tCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTGCGCCCTGTCGAAGCCCGCCTCTGCCAGTCGCCTTTCGAATAACGCCGGGCTGCCAAAt                   >  1:1436357/1‑150 (MQ=255)

                                                                                                                                     cACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTGCGCCCTGTCGAAGCCCGCCTCTGCCAGTCGCCTTTCGAATAACGCCGGGCTGCCAAATGc                 <  2:1896862/150‑1 (MQ=255)

                                                                                                                                       cTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTGCGCCCTGTCGAAGCCCGCCTCTGCCAGTCGCCTTTCGAATAACGCCGGGCTGCCAAATGCCg               >  1:923696/1‑150 (MQ=255)

                                                                                                                                           gCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTGCGCCCTGTCGAAGCCCGCCTCTGCCAGTCGCCTTTCGAATAACGCCGGGCTGCCAAATGCCGCTTc           <  1:2122878/150‑1 (MQ=255)

                                                                                                                                            gccctgctcaGCGCAGTCGAC‑GCGCCGAGCGAGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTGCGCCCTGTCGAAGCCCGCCTCTGCCAGTCGCCTTTCGAATAACGCCGGGCTGCCAAATGCCGCTTc           >  2:2713554/6‑150 (MQ=38)

                                                                                                                                            cccTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTGCGCCCTGTCGAAGCCCGCCTCTGCCAGTCGCCTTTCGAATAACGCCGGGCTGCCAAATGCCGCTTCg          >  1:1519607/1‑150 (MQ=255)

                                                                                                                                             ccTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTGCGCCCTGTCGAAGCCCGCCTCTGCCAGTCGCCTTTCGAATAACGCCGGGCTGCCAAATGCCGCTTCGa         >  1:1610200/1‑150 (MQ=255)

                                                                                                                                                 gctcgGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTGCGCCCTGTCGAAGCCCGCCTCTGCCAGTCGCCTTTCGAATAACGCCGGGCTGCCAAATGCCGCTTCGAtctc     <  2:3932152/150‑1 (MQ=255)

                                                                                                                                                  ctcgGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTGCGCCCTGTCGAAGCCCGCCTCTGCCAGTCGCCTTTCGAATAACGCCGGGCTGCCAAATGCCGCTTCGATCTCg    <  2:2363913/150‑1 (MQ=255)

                                                                                                                                                   tcgGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTGCGCCCTGTCGAAGCCCGCCTCTGCCAGTCGCCTTTCGAATAACGCCGGGCTGCCAAATGCCGCTTCGATCTCGc   >  2:1298208/1‑150 (MQ=255)

                                                                                                                                                    cgGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTGCGCCCTGTCGAAGCCCGCCTCTGCCAGTCGCCTTTCGAATAACGCCGGGCTGCCAAATGCCGCTTCGATCTCGcc  <  1:3363043/150‑1 (MQ=255)

                                                                                                                                                    cgGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTGCGCCCTGTCGAAGCCCGCCTCTGCCAGTCGCCTTTCGAATAACGCCGGGCTGCCAAATGCCGCTTCGATCTCGcc  >  2:4455531/1‑150 (MQ=255)

GCGCCTGCGCCTCCCGCTGCCCGATGAGCGTTGCCCGAGCCAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGGCATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGC‑GTCGACTGCGCTGAGC‑AGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTGCGCCCTGTCGAAGCCCGCCTCTGCCAGTCGCCTTTCGAATAACGCCGGGCTGCCAAATGCCGCTTCGATCTCGCC  >  NC_002947/3950914‑3951211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`