Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,051,703 T→C 5.3% V763A (GTG→GCG) ‡ quiA → quinate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,051,7030TC 5.3% 746.6 / 18.7 225V763A (GTG→GCG) ‡quiAquinate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (88/125);  new base C (4/8);  total (92/133)
Fisher's exact test for biased strand distribution p-value = 7.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01

Only 100 of 227 total aligned reads displayed.

CACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCG  >  NC_002947/4051555‑4051852
                                                                                                                                                    |                                                                                                                                                     
 aCCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTgg                                                                                                                                                     <  1:1050169/150‑1 (MQ=255)
  ccTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTggg                                                                                                                                                    <  2:4418074/150‑1 (MQ=255)
  caggCCGATCTTGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCACCGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTggg                                                                                                                                                    >  2:4169541/4‑150 (MQ=255)
    tGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCa                                                                                                                                                  <  2:1569640/150‑1 (MQ=255)
       cGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGcc                                                                                                                                               >  2:4276455/1‑150 (MQ=255)
        gATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCa                                                                                                                                              >  2:1811518/1‑150 (MQ=255)
         aTCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGGCTGCCCACGGGCAggcga                                                                                                                                             <  1:1774597/150‑5 (MQ=255)
         aTCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCACGGGCAggcga                                                                                                                                             <  2:3049985/150‑5 (MQ=255)
         aTCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCACGGGCAggcga                                                                                                                                             <  1:1415458/150‑5 (MQ=255)
          tCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAgg                                                                                                                                            >  1:2480832/1‑150 (MQ=255)
          tCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAgg                                                                                                                                            >  1:1971000/1‑150 (MQ=255)
           cAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAggg                                                                                                                                           <  1:3659733/150‑1 (MQ=255)
           cAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAggg                                                                                                                                           <  1:2072643/150‑1 (MQ=255)
              gATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGggcgg                                                                                                                                        >  2:1299234/1‑150 (MQ=255)
              gATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGggcgg                                                                                                                                        <  2:3837060/150‑1 (MQ=255)
              gATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGggcgg                                                                                                                                        <  2:1775468/150‑1 (MQ=255)
                   gCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGa                                                                                                                                   >  1:3780108/1‑150 (MQ=255)
                   gCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGa                                                                                                                                   >  1:775779/1‑150 (MQ=255)
                     cTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCCGCCCTGGCTGcccacgg                                                                                                                                 <  2:2547982/150‑5 (MQ=255)
                       gCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGAc                                                                                                                               >  2:2335717/1‑150 (MQ=255)
                       gCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGAc                                                                                                                               >  1:257792/1‑150 (MQ=255)
                        ccAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGAcc                                                                                                                              >  2:3710329/1‑150 (MQ=255)
                         cAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCt                                                                                                                             <  2:2534839/150‑1 (MQ=255)
                          aaCCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTa                                                                                                                            >  2:4226130/1‑150 (MQ=255)
                             ccTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCTCGATGACCTACGt                                                                                                                         >  2:2715013/1‑150 (MQ=255)
                             ccTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGt                                                                                                                         <  1:4371339/150‑1 (MQ=255)
                               ttGGCGGCACCCTGTCGACTCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCCGCCCGGGCGGCCCGCTGACCTACGtct                                                                                                                       >  1:3138094/1‑150 (MQ=255)
                                tGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGtctc                                                                                                                      <  2:1610477/150‑1 (MQ=255)
                                tGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGtctc                                                                                                                      <  1:3830894/150‑1 (MQ=255)
                                  gcggcACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGc                                                                                                                    <  2:3183933/150‑1 (MQ=255)
                                   cggcACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGcc                                                                                                                   <  1:1204595/150‑1 (MQ=255)
                                    ggcACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCg                                                                                                                  <  1:1265093/150‑1 (MQ=255)
                                    ggcACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCg                                                                                                                  >  1:3100804/1‑150 (MQ=255)
                                      cACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGTCAGGGCGGCCCGATGACCTACGTCTCGCCGaa                                                                                                                <  2:4085634/150‑1 (MQ=255)
                                        cccTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGa                                                                                                              <  2:692253/150‑1 (MQ=255)
                                          cTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGAcc                                                                                                            >  1:500207/1‑150 (MQ=255)
                                            gTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCgg                                                                                                          <  1:2795161/150‑1 (MQ=255)
                                             tCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGc                                                                                                         >  1:984658/1‑150 (MQ=255)
                                             tCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGc                                                                                                         <  1:4139297/150‑1 (MQ=255)
                                               gACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCaa                                                                                                       <  1:2301995/150‑1 (MQ=255)
                                                 cccAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGc                                                                                                     <  2:3603852/150‑1 (MQ=255)
                                                  ccAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCa                                                                                                    <  1:3679077/150‑1 (MQ=255)
                                                   cAAGGCGGCCTGGTGTTCATCGCAGGTGCCCTGGACTTCTTCGGGCGCGCCTACGCCAGCGGCAACGGTCACGAAAGCTGGAAGGCTCGCCGGGCCGTGGGCAGCCCGGGCGGCCCGTTGTCCGTCGTCTCGGCGAAGGCCGGCCCGCAg                                                                                                   >  2:2988083/1‑150 (MQ=255)
                                                         ggcCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACgtg                                                                                             <  2:2771369/150‑1 (MQ=255)
                                                         ggcCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACgtg                                                                                             <  1:45468/150‑1 (MQ=255)
                                                          gcCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACgtgg                                                                                            <  1:4495313/150‑1 (MQ=255)
                                                          gcCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACgtgg                                                                                            <  1:251875/150‑1 (MQ=255)
                                                           ccTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACgtggt                                                                                           >  1:3339424/1‑150 (MQ=255)
                                                             tGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGa                                                                                         <  2:1105041/150‑1 (MQ=255)
                                                             tGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGa                                                                                         <  1:1480254/150‑1 (MQ=255)
                                                                  ttCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCAct                                                                                    >  2:2372444/1‑150 (MQ=255)
                                                                  ttCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCAct                                                                                    <  1:190580/150‑1 (MQ=255)
                                                                  ttCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCAct                                                                                    >  2:2924506/1‑150 (MQ=255)
                                                                      tCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCActgctg                                                                                >  2:922683/1‑150 (MQ=255)
                                                                      tCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCActgctg                                                                                >  2:4033707/1‑150 (MQ=255)
                                                                               cccAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTCCGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGcgccc                                                                       <  1:759671/150‑1 (MQ=255)
                                                                               cccAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGcgccc                                                                       <  2:1334224/150‑1 (MQ=255)
                                                                               cccAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGcgccc                                                                       <  1:2285526/150‑1 (MQ=255)
                                                                               cccAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTACTggcgggcgcc                                                                       <  1:3600647/150‑6 (MQ=255)
                                                                                ccAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGcgcccg                                                                      >  1:2090437/1‑150 (MQ=255)
                                                                                ccAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGcgcccg                                                                      >  2:2099453/1‑150 (MQ=255)
                                                                                 cAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGcgcccgc                                                                     <  1:488075/150‑1 (MQ=255)
                                                                                  aGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGcgcccgcc                                                                    >  1:2572811/1‑150 (MQ=255)
                                                                                  aGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGcgcccgcc                                                                    >  2:2559829/1‑150 (MQ=255)
                                                                                   ggACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCa                                                                   <  2:1484929/150‑1 (MQ=255)
                                                                                   ggACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCa                                                                   <  1:464777/150‑1 (MQ=255)
                                                                                    gACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAg                                                                  >  2:337172/1‑150 (MQ=255)
                                                                                      cTTCTACCTGCGTGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTACTGGCGGGCGCCGCCAgca                                                                <  2:3684796/150‑3 (MQ=255)
                                                                                      cTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGGCGCCGCCAgca                                                                <  2:4360163/150‑3 (MQ=255)
                                                                                       ttCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCg                                                               >  1:1172600/1‑150 (MQ=255)
                                                                                       ttCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCg                                                               >  1:1961683/1‑150 (MQ=255)
                                                                                         cTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGAc                                                             <  2:3163222/150‑1 (MQ=255)
                                                                                         cTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGAc                                                             >  2:3769378/1‑150 (MQ=255)
                                                                                          tACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACt                                                            >  2:2630559/1‑150 (MQ=255)
                                                                                            ccTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGa                                                          <  1:2748075/150‑1 (MQ=255)
                                                                                                  cgcCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTgg                                                    <  1:697580/150‑1 (MQ=255)
                                                                                                   gcCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGc                                                   <  2:2357614/150‑1 (MQ=255)
                                                                                                       aCGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACt                                               <  2:1888029/150‑1 (MQ=255)
                                                                                                        cGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTa                                              <  2:2572830/150‑1 (MQ=255)
                                                                                                          aCAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTAtg                                            <  1:1388253/150‑1 (MQ=255)
                                                                                                              cagcaACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGAt                                        <  1:4033707/150‑1 (MQ=255)
                                                                                                                 caACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGTGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTc                                     <  1:1801567/150‑1 (MQ=255)
                                                                                                                 caACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTc                                     >  1:1883094/1‑150 (MQ=255)
                                                                                                                  aaCGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCt                                    <  1:4469226/150‑1 (MQ=255)
                                                                                                                   aCGGTAACGAAATCTGGTAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCtt                                   <  1:3884662/150‑1 (MQ=255)
                                                                                                                    cGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTa                                  <  1:2587493/150‑1 (MQ=255)
                                                                                                                    cGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTa                                  <  1:2175555/150‑1 (MQ=255)
                                                                                                                    cGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTa                                  <  2:4085924/150‑1 (MQ=255)
                                                                                                                    cGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTa                                  <  1:4215045/150‑1 (MQ=255)
                                                                                                                     ggTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTAc                                 <  2:3511170/150‑1 (MQ=255)
                                                                                                                             aaTCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGcc                         <  1:2171719/150‑1 (MQ=255)
                                                                                                                             aaTCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGcc                         <  1:3303827/150‑1 (MQ=255)
                                                                                                                                   gAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTaaaa                   <  2:1918982/150‑1 (MQ=255)
                                                                                                                                       gCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACcgc               <  1:548118/150‑1 (MQ=255)
                                                                                                                                       gCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACcgc               >  1:3579846/1‑150 (MQ=255)
                                                                                                                                       gCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACcgc               <  2:546367/150‑1 (MQ=255)
                                                                                                                                         tCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGt             >  1:60395/1‑150 (MQ=255)
                                                                                                                                            ccTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGc          >  2:2258332/1‑150 (MQ=255)
                                                                                                                                            ccTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGc          <  2:1971000/150‑1 (MQ=255)
                                                                                                                                            ccTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGc          <  2:2441154/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CACCTGCCGATCAAGATCGGCCTGCCAACCCTTGGCGGCACCCTGTCGACCCAAGGCGGCCTGGTGTTCATCGCAGGTACCCAGGACTTCTACCTGCGCGCCTACGACAGCAGCAACGGTAACGAAATCTGGAAGGCTCGCCTGCCCGTGGGCAGCCAGGGCGGCCCGATGACCTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCG  >  NC_002947/4051555‑4051852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑