BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,313,280	T→A	5.6%	F12I (TTT→ATT) ‡	PP_3784 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,313,280	0	T	A	5.6%	735.6 / 19.8	219	F12I (TTT→ATT) ‡	PP_3784	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (107/98); new base A (10/3); total (118/101)
Fisher's exact test for biased strand distribution p-value = 9.39e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 227 total aligned reads displayed.

GGACACAACCGCGTGCGCTCGCAAACCACTCGTGGCAAGGCGTTTAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCAACGAACAGGCACCACTTGGCGTGCTGCGTCTTGTCGTGGCAGTGAG  >  NC_002947/4313131‑4313428

 gACACAACCGCGTGCGCTCGCAAACCACTCGTGGCAAGGCGTTTAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCAtt                                                                                                                                                     <  1:3232148/150‑1 (MQ=255)

  acacAACCGCGTGCGCTCGCAAACCACTCGTGGCAAGGCGTTTAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCAttt                                                                                                                                                    <  1:1189374/150‑1 (MQ=255)

  acacAACCGCGTGCGCTCGCAAACCACTCGTGGCAAGGCGTTTAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCAttt                                                                                                                                                    >  2:2857847/1‑150 (MQ=255)

  acacAACCGCGTGCGCTCGCAAACCACTCGTGGCAAGGCGTTTAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCAttt                                                                                                                                                    >  1:3706732/1‑150 (MQ=255)

    acaACCGCGTGCGCTCGCAAACCACTCGTGGCAAGGCGTTTAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGc                                                                                                                                                  <  2:2192238/150‑1 (MQ=255)

    acaACCGCGTGCGCTCGCAAACCACTCGTGGCAAGGCGTTTAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGc                                                                                                                                                  <  1:2402577/150‑1 (MQ=255)

      aaCCGCGTGCGCTCGCAAACCACTCGTGGCAAGGCGTTTAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCTGGGGCACCGCATTTGCGt                                                                                                                                                >  2:2391799/1‑150 (MQ=255)

      aaCCGCGTGCGCTCGCAAACCACTCGTGGCAAGGCGTTTAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGt                                                                                                                                                >  1:1223490/1‑150 (MQ=255)

      aaCCGCGTGCGCTCGCAAACCACTCGTGGCAAGGCGTTTAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGt                                                                                                                                                >  1:1471505/1‑150 (MQ=255)

      aaCCGCGTGCGCTCGCAAACCACTCGTGGCAAGGCGTTTAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGt                                                                                                                                                >  1:3473852/1‑150 (MQ=255)

      aaCCGCGTGCGCTCGCAAACCACTCGTGGCAAGGCGTTTAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGt                                                                                                                                                >  2:758425/1‑150 (MQ=255)

      aaCCGCGTGCGCTCGCAAACCACTCGTGGCAAGGCGTTTAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGt                                                                                                                                                >  1:2029919/1‑150 (MQ=255)

        ccGCGTGCGCTCGCAAACCACTCGTGGCAAGGCGTTTAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCAAATGCGTTg                                                                                                                                              <  1:2760834/150‑1 (MQ=255)

          gcgTGCGCTCGCAAACCACTCGTGGCAAGGCGTTTAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGcc                                                                                                                                            <  1:1351851/150‑1 (MQ=255)

          gcgTGCGCTCGCAAACCACTCGTGGCAAGGCGTTTAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGcc                                                                                                                                            <  2:3067063/150‑1 (MQ=255)

          ccgTGCGCTCGCAAACCACTCGTGGCAACGCGATTAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATCGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGcc                                                                                                                                            <  2:4461674/149‑1 (MQ=255)

            gTGCGCTCGCAAACCACTCGTGGCAAGGCGTTTAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCt                                                                                                                                          >  1:2654261/1‑150 (MQ=255)

              gcgcTCGCAAACCACTCGTGGCAAGGCGTTTAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTc                                                                                                                                        <  2:2573676/150‑1 (MQ=255)

                gcTCGCAAACCACTCGTGGCAAGGCGTTTAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCACCGCAATTGCGTTGCCCTTCGc                                                                                                                                      >  1:4197570/1‑150 (MQ=255)

                gcTCGCAAACCACTCGTGGCAAGGCGTTTAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGc                                                                                                                                      >  1:3313720/1‑150 (MQ=255)

                  tCGCAAACCACTCGTGGCAAGGCGTTTAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGACAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCg                                                                                                                                    >  2:3251307/1‑150 (MQ=255)

                          cACTCGTGGCAAGGCGTTTAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGt                                                                                                                            >  1:1100959/1‑150 (MQ=255)

                             tCGTGGCAAGGCGTTTAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGtcat                                                                                                                         >  1:3615639/1‑150 (MQ=255)

                             tCGTGGCAAGGCGTTTAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGtcat                                                                                                                         <  1:1036398/150‑1 (MQ=255)

                             tCGTGGCAAGGCGTTTAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGtcat                                                                                                                         >  2:2294547/1‑150 (MQ=255)

                             tCGTGGCAAGGCGTTTAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGtcat                                                                                                                         >  1:302039/1‑150 (MQ=255)

                             tCGTGGCAAGGCGTTTAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGtcat                                                                                                                         <  2:3978754/150‑1 (MQ=255)

                                 ggCAAGGCGTTTAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAAt                                                                                                                     <  2:1077334/150‑1 (MQ=255)

                                  gCAAGGCGTTTAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAAtt                                                                                                                    >  2:1522007/1‑150 (MQ=255)

                                   cAAGGCGTTTAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATtc                                                                                                                   <  2:1021152/150‑1 (MQ=255)

                                    aaGGCGTTTAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATtca                                                                                                                  <  1:3265366/150‑1 (MQ=255)

                                    aaGGCGTTTAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATtca                                                                                                                  >  1:1318897/1‑150 (MQ=255)

                                    aaGGCGTTTAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATtca                                                                                                                  >  1:1675064/1‑150 (MQ=255)

                                     aGGCGTTTAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATtcat                                                                                                                 <  2:3464101/150‑1 (MQ=255)

                                       gCGTTTAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATtcatca                                                                                                               <  1:1003588/150‑1 (MQ=255)

                                       gCGTTTAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATtcatca                                                                                                               <  1:3849686/150‑1 (MQ=255)

                                          tttAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTg                                                                                                            >  1:4060984/1‑150 (MQ=255)

                                              aCCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCAc                                                                                                        <  2:4039462/150‑1 (MQ=255)

                                              aCCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCAc                                                                                                        <  2:1758039/150‑1 (MQ=255)

                                              aCCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCAc                                                                                                        <  2:85426/150‑1 (MQ=255)

                                               ccGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACg                                                                                                       >  2:4260872/1‑150 (MQ=255)

                                               ccGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACg                                                                                                       <  1:1068889/150‑1 (MQ=255)

                                               ccGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACg                                                                                                       >  1:2453291/1‑150 (MQ=255)

                                                cGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACgt                                                                                                      <  2:1901038/150‑1 (MQ=255)

                                                     tCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACg                                                                                                 >  1:2331360/1‑150 (MQ=255)

                                                      ccGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGc                                                                                                <  2:1511525/150‑1 (MQ=255)

                                                        gTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCt                                                                                              >  1:4344875/1‑150 (MQ=255)

                                                         tAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTg                                                                                             >  1:2785609/1‑150 (MQ=255)

                                                               tctGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGTCACAt                                                                                       >  2:3338679/1‑150 (MQ=255)

                                                                 tGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTc                                                                                     >  2:2860802/1‑150 (MQ=255)

                                                                    cccccGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGc                                                                                  <  2:3778372/150‑1 (MQ=255)

                                                                      cccGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGa                                                                                >  2:1694392/1‑150 (MQ=255)

                                                                       ccGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACTTGTACGCCTGACACATTCAGCGAc                                                                               <  1:703857/150‑1 (MQ=255)

                                                                            tCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCt                                                                          >  2:3569354/1‑150 (MQ=255)

                                                                                 tGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGc                                                                     <  1:208954/150‑1 (MQ=255)

                                                                                         cTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTg                                                             <  2:4517575/150‑1 (MQ=255)

                                                                                          tCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCGTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTgc                                                            >  1:3807282/1‑150 (MQ=255)

                                                                                               tCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCa                                                       >  2:273752/1‑150 (MQ=255)

                                                                                                 aGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATc                                                     >  2:1193348/1‑150 (MQ=255)

                                                                                                   tGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCgg                                                                                                                                   >  2:1176560/1‑70 (MQ=255)

                                                                                                     aaaGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGc                                                 >  2:1700197/1‑150 (MQ=255)

                                                                                                      aaGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCa                                                >  2:1652417/1‑150 (MQ=255)

                                                                                                       aGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCaa                                               >  2:295684/1‑150 (MQ=255)

                                                                                                               catcaATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCAACGAACAgg                                       <  2:4179639/150‑1 (MQ=255)

                                                                                                               catcaATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCAACGAACAgg                                       >  1:3568739/1‑150 (MQ=255)

                                                                                                                 tcaATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCAACGAACAGGca                                     <  2:1471570/150‑1 (MQ=255)

                                                                                                                 tcaATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCGTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAAACATTGCCGCCAACCAGCAACGAACAGGca                                     >  1:1493518/1‑150 (MQ=255)

                                                                                                                  caATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATACAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCAACGAACAGGcac                                    <  1:565818/150‑1 (MQ=255)

                                                                                                                   aaTGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCAACGAACAGGcacc                                   >  2:3695803/1‑150 (MQ=255)

                                                                                                                     tGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCAACGAACAGGcaccac                                 <  1:1998519/150‑1 (MQ=255)

                                                                                                                     tGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACAATCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCAACGAACAGGcaccac                                 >  1:228040/1‑150 (MQ=255)

                                                                                                                          aaCAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCAACGAACAGGCACCACTTGGc                            <  1:1700197/150‑1 (MQ=255)

                                                                                                                            cAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCAACGAACAGGCACCACTTGGCGt                          <  1:376362/150‑1 (MQ=255)

                                                                                                                            cAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCAACGAACAGGCACCACTTGGCGt                          <  2:3665683/150‑1 (MQ=255)

                                                                                                                             aGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCAACGAACAGGCACCACTTGGCGtg                         <  1:805640/150‑1 (MQ=255)

                                                                                                                              gCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCAACGAACAGGCACCACTTGGCGtgc                        <  2:2918472/150‑1 (MQ=255)

                                                                                                                              gCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCAACGAACAGGCACCACTTGGCGtgc                        <  2:445320/150‑1 (MQ=255)

                                                                                                                                aTTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCCTCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCAACGAACAGGCACCACTTGGCGtgctg                      >  2:386069/1‑150 (MQ=255)

                                                                                                                                 ttCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCAACGAACAGGCACCACTTGGCGtgctgc                     <  2:2654261/150‑1 (MQ=255)

                                                                                                                                   cccTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCAACGAACAGGCACCACTTGGCGTGCTGCGt                   <  2:2229560/150‑1 (MQ=255)

                                                                                                                                    ccTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCAACGAACAGGCACCACTTGGCGTGCTGCGTc                  <  2:4165173/150‑1 (MQ=255)

                                                                                                                                    ccTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCAACGAACAGGCACCACTTGGCGTGCTGCGTc                  <  2:1429440/150‑1 (MQ=255)

                                                                                                                                     cTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCAACGAACAGGCACCACTTGGCGTGCTGCGTCt                 >  2:971830/1‑150 (MQ=255)

                                                                                                                                     cTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCAACGAACAGGCACCACTTGGCGTGCTGCGTCt                 >  2:738103/1‑150 (MQ=255)

                                                                                                                                     cTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCAACGAACAGGCACCACTTGGCGTGCTGCGTCt                 >  2:3054805/1‑150 (MQ=255)

                                                                                                                                     cTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCAACGAACAGGCACCACTTGGCGTGCTGCGTCt                 >  2:1201622/1‑150 (MQ=255)

                                                                                                                                        gaagggCAACGCAAATGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCAACGAACAGGCACCACTTGGCGTGCTGCGTCTTGt              >  1:616129/4‑150 (MQ=255)

                                                                                                                                         gggggCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCAACGAACAGGCACCACTTGGCGTGCTGCGTCTTGTc             >  2:3578517/1‑150 (MQ=255)

                                                                                                                                           gggCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCAACGAACAGGCACCACTTGGCGTGCTGCGTCTTGTCGt           <  1:3858826/150‑1 (MQ=255)

                                                                                                                                            ggCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCAACGAACAGGCACCACTTGGCGTGCTGCGTCTTGTCGTg          <  1:2616861/150‑1 (MQ=255)

                                                                                                                                            ggCAACGCAAATGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCAACGAACAGGCACCACTTGGCGTGCTGCGTCTTGTCGTg          >  1:1991489/1‑150 (MQ=255)

                                                                                                                                             gCAACGCAAATGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCAACGAACAGGCACCACTTGGCGTGCTGCGTCTTGTCGTgg         >  1:2542389/1‑150 (MQ=255)

                                                                                                                                             gCAACGCAAATGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCAACGAACAGGCACCACTTGGCGTGCTGCGTCTTGTCGTgg         >  2:4370418/1‑150 (MQ=255)

                                                                                                                                              cAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCAACGAACAGGCACCACTTGGCGTGCTGCGt                   >  1:479958/1‑139 (MQ=255)

                                                                                                                                              cAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCAACGAACAGGCACCACTTGGCGTGCTGCGt                   <  2:479958/139‑1 (MQ=255)

                                                                                                                                              cAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCAACGAACAGGCACCACTTGGCGTGCTGCGTCTTGTCGTGGc        <  1:1646179/150‑1 (MQ=255)

                                                                                                                                                aCGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCAACGAACAGGCACCACTTGGCGTGCTGCGTCTTGTCGTGGCAg      <  1:795757/150‑1 (MQ=255)

                                                                                                                                                aCGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCAACGAACAGGCACCACTTGGCGTGCTGCGTCTTGTCGTGGCAg      >  2:824008/1‑150 (MQ=255)

                                                                                                                                                   cATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCAACGAACAGGCACCACTTGGCGTGCTGCGTCTTGTCGTGGCAGTGa   <  2:4143746/150‑1 (MQ=255)

                                                                                                                                                   cATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCAACGAACAGGCACCACTTGGCGTGCTGCGTCTTGTCGTGGCAGTGa   <  2:2029919/150‑1 (MQ=255)

GGACACAACCGCGTGCGCTCGCAAACCACTCGTGGCAAGGCGTTTAACCGAATTCCGTAACGCTCTGGCCCCCGAATCGATTGCGCGCCCTCCCGTCAGTGAAAGGAAGATCATCAATGGCTAACAGCATTCCCTCCGGGGGCAACGCATTTGCGTTGCCCTTCGCCGGCTCTCGTCATCAATTCATCAGTGCCACGTGTACGCCTGACACATTCAGCGACTGCCTGTCGCAATCATTGCCGCCATCCAGCAACGAACAGGCACCACTTGGCGTGCTGCGTCTTGTCGTGGCAGTGAG  >  NC_002947/4313131‑4313428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`