BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,377,410	A→T	5.5%	V31D (GTC→GAC)	PP_5637 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,377,410	0	A	T	5.5%	815.4 / 25.2	235	V31D (GTC→GAC)	PP_5637	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (112/110); new base T (10/3); total (122/113)
Fisher's exact test for biased strand distribution p-value = 8.64e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.51e-01

Only 100 of 237 total aligned reads displayed.

ACCACGACTGCGCATATAGCACGCCTTCGACCTCTTCTACCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTGTATAAA  >  NC_002947/4377261‑4377558

accacGACTGCGCATATAGCACGCCTTCGACCTCTTCTACCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGa                                                                                                                                                      >  2:150685/1‑150 (MQ=255)

 ccacGACTGCGCATATAGCACGCCTTCGACCTCTTCTACCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGAc                                                                                                                                                     >  2:3669945/1‑150 (MQ=255)

   acGACTGCGCATATAGCACGCCTTCGACCTCTTCTACCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTc                                                                                                                                                   <  2:1397659/150‑1 (MQ=255)

   acGACTGCGCATATAGCACGCCTTCGACCTCTTCTACCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTc                                                                                                                                                   >  2:543073/1‑150 (MQ=255)

          cgcATATAGCACGCCTTCGACCTCTTCTACCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGAGTCGCGACCCag                                                                                                                                            <  1:3898763/150‑1 (MQ=255)

           gcATATAGCACGCCTTCGACCTCTTCTACCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCaga                                                                                                                                           <  1:2900702/150‑1 (MQ=255)

            cATATAGCACGCCTTCGACCTCTTCTACCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCagag                                                                                                                                          >  1:3488317/1‑150 (MQ=255)

            cATATAGCACGCCTTCGACCTCTTCTACCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCagag                                                                                                                                          <  1:2578275/150‑1 (MQ=255)

             atatAGCACGCCTTCGACCTCTTCTACCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCT‑GGTCGCGACTCGACCCAGAGGc                                                                                                                                        >  2:913173/1‑150 (MQ=255)

               ataGCACGCCTTCGACCTCTTCTACCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCt                                                                                                                                       <  2:600712/150‑1 (MQ=255)

                 aGCACGCCTTCGACCTCTTCTACCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCg                                                                                                                                     >  1:952714/1‑150 (MQ=255)

                     cGCCTTCGACCTCTTCTACCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCtgt                                                                                                                                 <  1:3231299/150‑1 (MQ=255)

                       ccTTCGACCTCTTCTACCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGa                                                                                                                               <  2:2274443/150‑1 (MQ=255)

                       ccTTCGACCTCTTCTACCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGa                                                                                                                               <  2:2717159/150‑1 (MQ=255)

                       cattCTACCTCTTCTACCCCTTTTATGTTGATGCCTAGCTGGGCAATGCCTTTGCCCTTCGCATGGTGCAGCGGGGGGATAATTTCGGGCCCAGGGGTTGGGTTAAACACCCAGGCCTGGGACGCGACTCGACCCAGAGGCTCGCTGTGa                                                                                                                               <  1:1881373/148‑1 (MQ=255)

                        cTTCGACCTCTTCTACCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGAt                                                                                                                              <  2:4446391/150‑1 (MQ=255)

                        cTTCGACCTCTTCTACCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGAt                                                                                                                              >  1:3066203/1‑150 (MQ=255)

                         ttCGACCTCTTCTACCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATg                                                                                                                             >  2:3476657/1‑150 (MQ=255)

                         ttCGACCTCTTCTACCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCAGCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATg                                                                                                                             >  2:3917537/1‑150 (MQ=255)

                            gACCTCTTCTACCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTc                                                                                                                          <  2:328422/150‑1 (MQ=255)

                            gACCTCTTCTACCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTc                                                                                                                          >  2:201825/1‑150 (MQ=255)

                             aCCTCTTCTACCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCg                                                                                                                         >  2:4397353/1‑150 (MQ=255)

                              ccTCTTCTACCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGc                                                                                                                        >  1:3599131/1‑150 (MQ=255)

                              ccTCTTCTACCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGc                                                                                                                        <  2:2525666/150‑1 (MQ=255)

                              ccTCTTCTACCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGc                                                                                                                        >  2:514310/1‑150 (MQ=255)

                               ctctTCTACCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGcc                                                                                                                       >  1:2546477/1‑150 (MQ=255)

                                    ctACCCCGTTGATTTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGTATAATGTCGGGCCCAGGCGTCGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAAtgt                                                                                                                  <  1:748770/150‑1 (MQ=255)

                                      aCCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTgg                                                                                                                <  1:2732651/150‑1 (MQ=255)

                                       ccccGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGa                                                                                                               >  2:2253894/1‑150 (MQ=255)

                                       ccccGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGa                                                                                                               >  1:4010964/1‑150 (MQ=255)

                                           gttgatgttgatGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTc                                                                                                           >  1:1005226/1‑150 (MQ=255)

                                            ttgatgttgatgCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTcc                                                                                                          <  2:434113/150‑1 (MQ=255)

                                                tgttgatgCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGCCGCCAATGTGGACGTCcgcgc                                                                                                      <  2:3151944/150‑1 (MQ=255)

                                                  ttgatgCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCgg                                                                                                    >  2:1283081/1‑150 (MQ=255)

                                                   tgatgCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGa                                                                                                   >  2:4308254/1‑150 (MQ=255)

                                                   tgatgCCGAGCTGGCCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGa                                                                                                   <  1:1704892/150‑1 (MQ=255)

                                                      tgCCGAGCTGGGCCATGCCGTTGACCTTCGCATGGTGCTGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCTGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAggg                                                                                                <  1:963211/150‑1 (MQ=255)

                                                      tgCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAggg                                                                                                >  2:3714278/1‑150 (MQ=255)

                                                       gCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAgggg                                                                                               >  1:50422/1‑150 (MQ=255)

                                                         cGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCt                                                                                             <  2:2491272/150‑1 (MQ=255)

                                                         cGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCt                                                                                             >  2:1635099/1‑150 (MQ=255)

                                                                  ccATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTcc                                                                                    <  1:2528407/150‑1 (MQ=255)

                                                                        cggtgcccTTCGCCTCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAACCACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGc                                                                              <  2:3009668/144‑1 (MQ=255)

                                                                         gTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCg                                                                             >  1:2468369/1‑150 (MQ=255)

                                                                             aCCTTCGCATCGGGCAGCCGGGGGAGAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCt                                                                         <  2:2667003/150‑1 (MQ=255)

                                                                               cTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGa                                                                       <  1:3753929/150‑1 (MQ=255)

                                                                               cTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGa                                                                       >  1:3709187/1‑150 (MQ=255)

                                                                               cTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGa                                                                       >  1:1334211/1‑150 (MQ=255)

                                                                               cTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGa                                                                       >  2:1338105/1‑150 (MQ=255)

                                                                                     aTCGTGCAGCCGGTGGATAATGTCGTGCCCAGGAGTAGGGTTAAACACCCAGGCCTTGTTCGAGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTACGTAGGCGGTCTGATGGCaa                                                                 >  1:292482/1‑150 (MQ=255)

                                                                                      tCGTGCAGCCGGGGTATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAg                                                                <  1:2684243/150‑1 (MQ=255)

                                                                                       cGTGCAGCCGGGGGATAATGTCGGGCCCCGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAgg                                                               <  1:1402233/150‑1 (MQ=255)

                                                                                       cGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCGGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAgg                                                               <  1:5435/150‑1 (MQ=255)

                                                                                        gTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGc                                                              <  1:4479349/150‑1 (MQ=255)

                                                                                            aGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACg                                                          <  2:2606659/150‑1 (MQ=255)

                                                                                            aGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACg                                                          >  1:2269758/1‑150 (MQ=255)

                                                                                             gCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGc                                                         >  1:829845/1‑150 (MQ=255)

                                                                                               cGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCa                                                       <  1:150685/150‑1 (MQ=255)

                                                                                                gggggATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCAt                                                      >  2:49089/1‑150 (MQ=255)

                                                                                                gggggATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCAt                                                      >  2:2956090/1‑150 (MQ=255)

                                                                                                   ggATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCt                                                   >  2:3268397/1‑150 (MQ=255)

                                                                                                    gATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTc                                                  >  2:649352/1‑150 (MQ=255)

                                                                                                    gATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTGTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTc                                                  >  2:3685958/1‑150 (MQ=255)

                                                                                                      tAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTcgc                                                <  2:1781923/150‑1 (MQ=255)

                                                                                                      tAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTcgc                                                <  1:2187368/150‑1 (MQ=255)

                                                                                                        aTGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCgcgg                                              <  2:2824272/150‑1 (MQ=255)

                                                                                                           tCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGt                                           <  2:1217369/150‑1 (MQ=255)

                                                                                                           tCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGt                                           <  1:1872121/150‑1 (MQ=255)

                                                                                                             gggCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCg                                         >  1:3117968/1‑150 (MQ=255)

                                                                                                              ggCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGa                                        >  1:1745205/1‑150 (MQ=255)

                                                                                                                cccAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAAc                                      <  2:1703914/150‑1 (MQ=255)

                                                                                                                 ccAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACg                                     >  1:1099216/1‑150 (MQ=255)

                                                                                                                  cAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACga                                    >  2:3230578/1‑150 (MQ=255)

                                                                                                                  cAGGCGTAGGGTTAAACACCCAGGCCTGGGCCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACga                                    >  2:1125698/1‑150 (MQ=255)

                                                                                                                     gCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAgaa                                 <  2:4232990/150‑1 (MQ=255)

                                                                                                                     gCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTCGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAgaa                                 <  1:2007386/150‑1 (MQ=255)

                                                                                                                      cGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAgaag                                <  2:3800074/150‑1 (MQ=255)

                                                                                                                         aGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAgaaggaa                             <  1:4258665/150‑1 (MQ=255)

                                                                                                                           ggTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGt                           <  2:631062/150‑1 (MQ=255)

                                                                                                                           ggTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGt                           >  1:2278192/1‑150 (MQ=255)

                                                                                                                            gTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTa                          >  1:2223060/1‑150 (MQ=255)

                                                                                                                             ttAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTAc                         >  2:8038/1‑150 (MQ=255)

                                                                                                                             ttAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTAc                         >  1:2808076/1‑150 (MQ=255)

                                                                                                                               aaaCACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACAt                       >  2:724027/1‑150 (MQ=255)

                                                                                                                                 acacCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGc                     <  2:3506544/150‑1 (MQ=255)

                                                                                                                                   acCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCgg                   <  2:1946505/150‑1 (MQ=255)

                                                                                                                                     ccAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCa                 >  1:239843/1‑150 (MQ=255)

                                                                                                                                        ggCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCa              <  1:3780406/150‑1 (MQ=255)

                                                                                                                                        cctcTGGGTCGAGTCGCGACCCAGGGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCa              >  2:898407/4‑150 (MQ=255)

                                                                                                                                        cctcTGGGTCGAGTCGCGACCCAGGGGCTCGCGGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCa              >  2:3166711/4‑150 (MQ=255)

                                                                                                                                          tcTGGGTCGAGTCGCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGTGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAAt            >  2:2310882/2‑150 (MQ=255)

                                                                                                                                             gggTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACt         <  1:3669945/150‑1 (MQ=255)

                                                                                                                                             gggTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACt         >  2:3411641/1‑150 (MQ=255)

                                                                                                                                               gTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTGt       >  2:412198/1‑150 (MQ=255)

                                                                                                                                               gTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTGt       <  1:2713306/150‑1 (MQ=255)

                                                                                                                                               gTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTGt       <  1:1112740/150‑1 (MQ=255)

                                                                                                                                                tCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTGta      >  1:4277879/1‑150 (MQ=255)

                                                                                                                                                  gcgACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTGtata    >  1:1309715/1‑150 (MQ=255)

                                                                                                                                                   cgACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTGTATaa   <  1:1866338/150‑1 (MQ=255)

                                                                                                                                                    gACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTGTATaaa  <  2:1068621/150‑1 (MQ=255)

ACCACGACTGCGCATATAGCACGCCTTCGACCTCTTCTACCCCGTTGATGTTGATGCCGAGCTGGGCCATGCCGTTGACCTTCGCATCGTGCAGCCGGGGGATAATGTCGGGCCCAGGCGTAGGGTTAAACACCCAGGCCTGGGTCGCGACTCGACCCAGAGGCTCGCTGTGATGGTCGCCAATGTGGACGTCCGCGCGGAGGGGCTGGACTTTCCGTAGGCGGTCTGAGGGCAAGGCCACGCCATGCTCGCGGCGTCGAACGAGAAGGAAGTACATGCGGCACCAATACTGTATAAA  >  NC_002947/4377261‑4377558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`