Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,465,919 A→T 5.7% *139C (TGA→TGT)  PP_3956 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,465,9190AT 5.7% 845.8 / 26.8 246*139C (TGA→TGT) PP_3956hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (134/98);  new base T (5/9);  total (139/107)
Fisher's exact test for biased strand distribution p-value = 1.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

Only 100 of 251 total aligned reads displayed.

CCCGAGGCTGGTGACTACCAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTCCTGGCCCCCTTCTCGCAGATGCACTT  >  NC_002947/4465770‑4466068
                                                                                                                                                     |                                                                                                                                                     
cccGAGGCTGGTGACTACCAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGa                                                                                                                                                       >  2:1806120/1‑150 (MQ=255)
cccGAGGCTGGTGACTACCAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGa                                                                                                                                                       <  1:2209058/150‑1 (MQ=255)
cccGAGGCTGGTGACTACCAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGa                                                                                                                                                       <  1:4179512/150‑1 (MQ=255)
        tGGTGACTACCAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACa                                                                                                                                               >  1:2238221/1‑150 (MQ=255)
         ggTGACTACCAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAg                                                                                                                                              <  2:4262252/150‑1 (MQ=255)
           tGACTACCAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGTCGCCGTCAggg                                                                                                                                            <  2:3001545/150‑1 (MQ=255)
             aCTACCAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGTCCTGCCCTGTCGCCGTCAGGGCa                                                                                                                                          <  1:3800374/150‑1 (MQ=255)
              cTACCAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAg                                                                                                                                         <  2:1975918/150‑1 (MQ=255)
               tACCAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAgg                                                                                                                                        >  1:3021917/1‑150 (MQ=255)
               tACCAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAgg                                                                                                                                        >  1:131297/1‑150 (MQ=255)
                 ccAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGAc                                                                                                                                      >  2:1209092/1‑150 (MQ=255)
                  cAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGAcc                                                                                                                                     <  1:4057675/150‑1 (MQ=255)
                     gTCTGCATGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGaa                                                                                                                                  <  1:3685049/150‑1 (MQ=255)
                          cACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCAc                                                                                                                             <  2:2285784/150‑1 (MQ=255)
                          cACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCAc                                                                                                                             >  2:3535680/1‑150 (MQ=255)
                           aCGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACt                                                                                                                            >  1:1765752/1‑150 (MQ=255)
                              aCAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCa                                                                                                                         <  2:3755038/150‑1 (MQ=255)
                                 gACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGcc                                                                                                                      <  1:3049726/150‑1 (MQ=255)
                                 gACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGcc                                                                                                                      >  1:2166945/1‑150 (MQ=255)
                                  aCGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGccc                                                                                                                     <  1:953639/150‑1 (MQ=255)
                                     ggTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCccacc                                                                                                                  <  2:1496106/150‑1 (MQ=255)
                                      gTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCccacca                                                                                                                 <  1:1873119/150‑1 (MQ=255)
                                          cagccaCTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTg                                                                                                             <  2:1684071/150‑1 (MQ=255)
                                           agccaCTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTACAGCCCACCACGTGc                                                                                                            >  1:1591936/1‑150 (MQ=255)
                                           agccaCTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCGGCCCTGACGGCGGCAGGGCAGGACCGAATCCCCTTCAGCCCACCACGTgg                                                                                                            >  2:2440748/1‑149 (MQ=255)
                                             ccaCTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCaa                                                                                                          <  1:4286759/150‑1 (MQ=255)
                                              caCTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAg                                                                                                         >  2:235580/1‑150 (MQ=255)
                                              caCTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAg                                                                                                         >  1:3616493/1‑150 (MQ=255)
                                                 tGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTaa                                                                                                      >  2:2922192/1‑150 (MQ=255)
                                                     cTATTGGTTAGGACCGCCGAGTGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCa                                                                                                  >  1:81752/1‑150 (MQ=255)
                                                      tATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAg                                                                                                 >  2:1727001/1‑150 (MQ=255)
                                                      tATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAg                                                                                                 <  1:561292/150‑1 (MQ=255)
                                                      tATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAg                                                                                                 >  2:2057588/1‑150 (MQ=255)
                                                      tATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAg                                                                                                 <  1:1148053/150‑1 (MQ=255)
                                                      tATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAg                                                                                                 >  1:2645906/1‑150 (MQ=255)
                                                       aTTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAgg                                                                                                >  2:3303747/1‑150 (MQ=255)
                                                       aTTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAgg                                                                                                >  1:3914075/1‑150 (MQ=255)
                                                        ttGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGt                                                                                               >  1:1230286/1‑150 (MQ=255)
                                                        ttGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGt                                                                                               <  1:1797501/150‑1 (MQ=255)
                                                        ttGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGt                                                                                               <  1:2051654/150‑1 (MQ=255)
                                                           gTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGc                                                                                            <  1:3478922/150‑1 (MQ=255)
                                                           gTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGc                                                                                            >  1:4448853/1‑150 (MQ=255)
                                                           gTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGc                                                                                            >  1:3864041/1‑150 (MQ=255)
                                                               ggACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTc                                                                                        <  2:1577673/150‑1 (MQ=255)
                                                               ggACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTc                                                                                        <  1:1762483/150‑1 (MQ=255)
                                                                 aCCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGt                                                                                      <  1:616111/150‑1 (MQ=255)
                                                                  ccgccgAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTa                                                                                     <  1:2811736/150‑1 (MQ=255)
                                                                  ccgccgAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTa                                                                                     <  2:1318784/150‑1 (MQ=255)
                                                                   cgccgAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTAc                                                                                    <  1:2766021/150‑1 (MQ=255)
                                                                    gccgAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACt                                                                                   <  2:360793/150‑1 (MQ=255)
                                                                    gccgAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACt                                                                                   <  2:2772955/150‑1 (MQ=255)
                                                                     ccgAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTg                                                                                  >  1:1038577/1‑150 (MQ=255)
                                                                       gAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGAt                                                                                >  1:1629277/1‑150 (MQ=255)
                                                                          cGAACTCATTGCTGCAGTAGACTTCTGGGCCTTTGAATATCCGCCTTTGGACAGCAGGCGAACCGACCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCAACACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCa                                                                             <  1:124925/150‑1 (MQ=255)
                                                                          cGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCa                                                                             <  1:2356998/150‑1 (MQ=255)
                                                                           gAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAg                                                                            <  2:4369361/150‑1 (MQ=255)
                                                                           gAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAg                                                                            >  2:103896/1‑150 (MQ=255)
                                                                            aaCGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAgg                                                                           >  1:2287825/1‑150 (MQ=255)
                                                                                 aTTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTc                                                                      >  2:2485750/1‑150 (MQ=255)
                                                                                    gctgcAGTAGCCTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGcc                                                                   <  1:257702/150‑1 (MQ=255)
                                                                                         aGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATca                                                              <  2:436250/150‑1 (MQ=255)
                                                                                           tAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATcaca                                                            >  2:1436248/1‑150 (MQ=255)
                                                                                           tAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATcaca                                                            >  2:1870137/1‑150 (MQ=255)
                                                                                           tAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATcaca                                                            >  2:1831521/1‑150 (MQ=255)
                                                                                             gACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATc                                                          >  1:4254708/1‑150 (MQ=255)
                                                                                                ttCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTc                                                       >  2:627354/1‑150 (MQ=255)
                                                                                                 tCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCa                                                      >  1:2843443/1‑150 (MQ=255)
                                                                                                 tCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCa                                                      >  1:1536833/1‑150 (MQ=255)
                                                                                                    gggCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACgg                                                   >  2:723590/1‑150 (MQ=255)
                                                                                                     ggCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGc                                                  >  1:1070048/1‑150 (MQ=255)
                                                                                                     ggCCTGTGAAAATCCGCCTTTGGACAGCAGGAGAACCGCCCTGCCCTGACTGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGAGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGc                                                  >  1:500340/1‑150 (MQ=255)
                                                                                                      gCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCt                                                 >  2:2717261/1‑150 (MQ=255)
                                                                                                         tgtgAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCa                                              >  2:4319365/1‑150 (MQ=255)
                                                                                                         tgtgAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCa                                              <  2:3352340/150‑1 (MQ=255)
                                                                                                           tgAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCAcc                                            >  2:2742050/1‑150 (MQ=255)
                                                                                                             aaaaTCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCAcccc                                          <  2:3864069/150‑1 (MQ=255)
                                                                                                              aaaTCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCa                                         <  1:1679130/150‑1 (MQ=255)
                                                                                                              aaaTCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCa                                         <  1:1827101/150‑1 (MQ=255)
                                                                                                                 tCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCatga                                      >  1:3258417/1‑150 (MQ=255)
                                                                                                                  ccGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCatgat                                     >  1:1750324/1‑150 (MQ=255)
                                                                                                                    gCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGt                                   >  1:2346854/1‑150 (MQ=255)
                                                                                                                      cTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCg                                 <  1:3222272/150‑1 (MQ=255)
                                                                                                                      cTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCg                                 >  1:3140227/1‑150 (MQ=255)
                                                                                                                      cTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCg                                 >  1:2867586/1‑150 (MQ=255)
                                                                                                                        ttGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTa                               <  2:2221001/150‑1 (MQ=255)
                                                                                                                             cagcagGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTCCt                          <  2:4453396/150‑1 (MQ=255)
                                                                                                                               gcagGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTCCTgg                        <  2:4498907/150‑1 (MQ=255)
                                                                                                                                 agGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGAGGTCGTAGTCCTGGcc                      >  2:3692448/1‑150 (MQ=255)
                                                                                                                                   gCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTCCTGGcccc                    >  2:3066835/1‑150 (MQ=255)
                                                                                                                                   gCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTCCTGGcccc                    <  1:1201115/150‑1 (MQ=255)
                                                                                                                                     gAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTCCTGGCCCCCt                  >  1:2482087/1‑150 (MQ=255)
                                                                                                                                     gAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTCCTGGCCCCCt                  <  1:2990148/150‑1 (MQ=255)
                                                                                                                                     gAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTCCTGGCCCCCt                  >  1:1401025/1‑150 (MQ=255)
                                                                                                                                     gAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTCCTGGCCCCCt                  >  2:784909/1‑150 (MQ=255)
                                                                                                                                         cGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTCCTGGCCCCCTtctc              <  2:20928/150‑1 (MQ=255)
                                                                                                                                          gccctgccctGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTCCTGGCCCCCTTCTCg             >  1:2188372/1‑150 (MQ=255)
                                                                                                                                          gccctgccctGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTCCTGGCCCCCTTCTCg             <  2:3122826/150‑1 (MQ=255)
                                                                                                                                            cctgccctgACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGTTGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTCCTGGCCCCCTTCTCGCa           <  2:1413024/150‑1 (MQ=255)
                                                                                                                                              tgccctgTCGCCGTCAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTCCTGGCCCCCTTCTCGCAGa         >  1:1974999/1‑150 (MQ=255)
                                                                                                                                                     aCGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTCCTGGCCCCCTTCTCGCAGATGCACtt  >  2:4053223/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCCGAGGCTGGTGACTACCAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTCCTGGCCCCCTTCTCGCAGATGCACTT  >  NC_002947/4465770‑4466068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑