BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,545,563	G→A	5.5%	G11E (GGG→GAG)	rnz →	ribonuclease Z

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,545,563	0	G	A	5.5%	773.6 / 20.9	240	G11E (GGG→GAG)	rnz	ribonuclease Z
Reads supporting (aligned to +/- strand): ref base G (103/122); new base A (7/6); total (111/129)
Fisher's exact test for biased strand distribution p-value = 5.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 249 total aligned reads displayed.

CAGCAAGCTCATCTGGCGACAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCCGCGACCTGCGCGCGATCTTCATCA  >  NC_002947/4545414‑4545712

 aGCAAGCTCATCTGGCGACAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCggg                                                                                                                                                      <  1:3151362/150‑1 (MQ=255)

   cAAGCTCATCTGGCGACAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGt                                                                                                                                                    <  1:4134215/150‑1 (MQ=255)

    aaGCTCATCTGGCGACAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTg                                                                                                                                                   >  2:1887675/1‑150 (MQ=255)

     aGCTCATCTGGCGACAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGc                                                                                                                                                  <  1:862545/150‑1 (MQ=255)

      gCTCATCTGGCGACAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGcc                                                                                                                                                 >  1:1539483/1‑150 (MQ=255)

        tCATCTGGCGACAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCcca                                                                                                                                               >  1:3552300/1‑150 (MQ=255)

        tCATCTGGCGACAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCCCGGCGGAGGTGccaa                                                                                                                                               <  1:2264661/150‑3 (MQ=255)

        tCATCTGGCGACAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCCCGGCGGAGGTGccaa                                                                                                                                               <  1:766019/150‑3 (MQ=255)

          aTCTGGCGACAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCccacc                                                                                                                                             <  1:947194/150‑1 (MQ=255)

               gCGACAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGc                                                                                                                                        <  2:3500035/150‑1 (MQ=255)

               gCGACAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGc                                                                                                                                        <  1:2481673/150‑1 (MQ=255)

                cGACAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCa                                                                                                                                       <  1:2692247/150‑1 (MQ=255)

                 gACAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGACCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCAc                                                                                                                                      >  1:732559/1‑150 (MQ=255)

                     ggATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGAACGCaa                                                                                                                                  >  1:2103327/1‑150 (MQ=255)

                      gATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAAt                                                                                                                                 >  1:2840785/1‑150 (MQ=255)

                      gATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAAt                                                                                                                                 >  2:1240799/1‑150 (MQ=255)

                       aTAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATg                                                                                                                                >  2:1022898/1‑150 (MQ=255)

                          aCTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGTGTGCCCACCAAGGCACGCAATGTCa                                                                                                                             <  1:3837259/150‑1 (MQ=255)

                          aCTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCa                                                                                                                             <  1:3008268/150‑1 (MQ=255)

                           cTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAg                                                                                                                            >  1:3756971/1‑150 (MQ=255)

                             aGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAgcg                                                                                                                          >  2:3028739/1‑150 (MQ=255)

                                    ggTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGc                                                                                                                   >  2:1078250/1‑150 (MQ=255)

                                     gTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGcc                                                                                                                  <  1:2393885/150‑1 (MQ=255)

                                      tCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCg                                                                                                                 >  1:2772730/1‑150 (MQ=255)

                                      tCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCg                                                                                                                 >  1:999026/1‑150 (MQ=255)

                                      tCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCg                                                                                                                 >  2:336977/1‑150 (MQ=255)

                                         aCGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGa                                                                                                              <  1:2862897/150‑1 (MQ=255)

                                         aCGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGa                                                                                                              <  2:1729094/150‑1 (MQ=255)

                                          cggcggGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGAt                                                                                                             >  1:1825138/1‑150 (MQ=255)

                                          cggcggGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGAt                                                                                                             <  2:3899918/150‑1 (MQ=255)

                                          cggcggGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGAt                                                                                                             <  2:3450722/150‑1 (MQ=255)

                                           ggcggGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATc                                                                                                            <  2:3970552/150‑1 (MQ=255)

                                           ggcggGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATc                                                                                                            <  2:1984414/150‑1 (MQ=255)

                                           ggcggGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGGTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATc                                                                                                            <  2:435685/150‑1 (MQ=255)

                                              gggTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGaa                                                                                                         <  2:3067900/150‑1 (MQ=255)

                                              gggTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGaa                                                                                                         <  1:109274/150‑1 (MQ=255)

                                              gggTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGaa                                                                                                         <  1:1951904/150‑1 (MQ=255)

                                              gggTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGaa                                                                                                         <  1:3498576/150‑1 (MQ=255)

                                              gggTGGCAATCCGGCTAAGGTGTGCGCCCCGGATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGGTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGaa                                                                                                         <  2:3118948/150‑1 (MQ=255)

                                                gTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGc                                                                                                       >  2:1411356/1‑150 (MQ=255)

                                                gTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGc                                                                                                       <  2:1572810/150‑1 (MQ=255)

                                                     aaTCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCg                                                                                                  <  1:478692/150‑1 (MQ=255)

                                                     aaTCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCg                                                                                                  >  1:659179/1‑150 (MQ=255)

                                                      aTCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGGTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCgg                                                                                                 <  2:3090164/150‑1 (MQ=255)

                                                        ccGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCa                                                                                               <  2:2263466/150‑1 (MQ=255)

                                                          ggCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGc                                                                                             <  2:1392383/150‑1 (MQ=255)

                                                          ggCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGc                                                                                             <  1:4108417/150‑1 (MQ=255)

                                                           gCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGcc                                                                                            >  1:2973167/1‑150 (MQ=255)

                                                                 gtgtGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGt                                                                                      >  1:1401846/1‑150 (MQ=255)

                                                                    tgCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCCAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTg                                                                                        <  2:1628743/145‑1 (MQ=255)

                                                                        ccccGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGt                                                                               <  1:1881473/150‑1 (MQ=255)

                                                                               tCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTgc                                                                        >  2:716362/1‑150 (MQ=255)

                                                                                 aCCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTgcgc                                                                      <  2:1727923/150‑2 (MQ=255)

                                                                                  ccTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTgcggc                                                                     >  1:2623979/1‑150 (MQ=255)

                                                                                     tGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGaa                                                                  <  1:421368/150‑1 (MQ=255)

                                                                                     tGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGaa                                                                  <  1:3985436/150‑1 (MQ=255)

                                                                                     tGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGaa                                                                  <  2:4499585/150‑1 (MQ=255)

                                                                                     tGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGaa                                                                  <  1:1644410/150‑1 (MQ=255)

                                                                                      ggCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTCTCTGGTCGATTGCGGCGAAg                                                                 >  2:932643/1‑150 (MQ=255)

                                                                                       gCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAgg                                                                >  1:250804/1‑150 (MQ=255)

                                                                                          gCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCAc                                                             >  1:1373263/1‑150 (MQ=255)

                                                                                            ttCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCAccc                                                           >  2:2783928/1‑150 (MQ=255)

                                                                                             tCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCa                                                          <  1:1936297/150‑1 (MQ=255)

                                                                                                ggCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCa                                                       <  2:1519246/150‑1 (MQ=255)

                                                                                                      aaaCAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCct                                                 <  2:1100545/150‑1 (MQ=255)

                                                                                                         cAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCctgct                                              <  2:3889647/150‑1 (MQ=255)

                                                                                                                 tGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACAccc                                      >  2:137729/1‑150 (MQ=255)

                                                                                                                 tGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACAccc                                      >  2:3047973/1‑150 (MQ=255)

                                                                                                                       tGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCttt                                <  2:1351583/150‑1 (MQ=255)

                                                                                                                       tGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCttt                                <  1:2588102/150‑1 (MQ=255)

                                                                                                                        ggACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTc                               >  2:3284661/1‑150 (MQ=255)

                                                                                                                        ggACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTc                               <  2:4217250/150‑1 (MQ=255)

                                                                                                                        ggACCGGCTGTTCCGTGGCACCTGCGCCGGGGTGCGCACCAAGGCACGCAATGTCTGCGCGACAGCCGTGATCGAAGCCAGCGGCAGCCTCTGGTATCTGGTCGATTGCGGCGTAGGCACCCTGCTCCGCCTGCTGCACACCCCGCTTTc                               <  2:4376334/150‑1 (MQ=255)

                                                                                                                          aCCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGa                             >  2:1484857/1‑150 (MQ=255)

                                                                                                                              gctgTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCcg                         >  1:1672051/1‑150 (MQ=255)

                                                                                                                                tgTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCcgcg                       >  2:517719/1‑150 (MQ=255)

                                                                                                                                tgTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCcgcg                       >  1:1008405/1‑150 (MQ=255)

                                                                                                                                tgTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTGTCGATCcgcg                       >  2:1617579/1‑150 (MQ=255)

                                                                                                                                  ttggtgggCACCCCGGCGGAGGTG‑CCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCCGCGAcc                    >  2:871189/7‑150 (MQ=255)

                                                                                                                                  ttCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCCGCGAc                     >  2:3988445/1‑150 (MQ=255)

                                                                                                                                    ccTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCCGCGACCt                   >  1:3586359/1‑150 (MQ=255)

                                                                                                                                     cTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCCGCGACCTg                  >  1:3132636/1‑150 (MQ=255)

                                                                                                                                      ttGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCCGCGACCTgc                 >  2:2596660/1‑150 (MQ=255)

                                                                                                                                      ttGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCCGCGACCTgc                 >  1:3991876/1‑150 (MQ=255)

                                                                                                                                      ttGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCCGCGACCTgc                 <  2:4311158/150‑1 (MQ=255)

                                                                                                                                      ttGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCCGCGACCTgc                 >  1:1257821/1‑150 (MQ=255)

                                                                                                                                      tgggCACCCCGGCGGAGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCCGCACCGCCTGCTGCACACCCCGCTTTCGATCCGCGACCTgc                 >  2:2450901/3‑150 (MQ=255)

                                                                                                                                      tgggCACCCCGGCGGAGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCCGCGACCTgc                 >  1:4033334/3‑150 (MQ=255)

                                                                                                                                      tgggCACCCCGGCGGAGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCCGCGACCTgc                 >  2:681121/3‑150 (MQ=255)

                                                                                                                                      tgggCACCCCGGCGGAGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCAATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCCGCGACCTgc                 >  2:2200503/3‑150 (MQ=255)

                                                                                                                                         gCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCCGCGACCTgcgcg              >  1:3726282/1‑150 (MQ=255)

                                                                                                                                          cACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCCGCGACCTgcgcgc             <  1:4406440/150‑1 (MQ=255)

                                                                                                                                          cACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCCGCGACCTgcgcgc             <  1:3838631/150‑1 (MQ=255)

                                                                                                                                           aCCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCCGCGACCTGcgcgcg            >  1:159500/1‑150 (MQ=255)

                                                                                                                                           aCCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCCGCGACCTGcgcgcg            >  2:2390466/1‑150 (MQ=255)

                                                                                                                                              tCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCCGCGACCTGCGCGCGATc         >  1:2558140/1‑150 (MQ=255)

                                                                                                                                              tCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCCGCGACCTGCGCGCGATc         <  2:3552300/150‑1 (MQ=255)

                                                                                                                                                 gccgGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCCGCGACCTGCGCGCGATCTtc      <  2:4099964/150‑1 (MQ=255)

                                                                                                                                                    ggggTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCCGCGACCTGCGCGCGATCTtcatc   <  1:336977/150‑1 (MQ=255)

                                                                                                                                                     gggTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCCGCGACCTGCGCGCGATCTtcatca  >  2:4431152/1‑150 (MQ=255)

CAGCAAGCTCATCTGGCGACAGGATAACTAGCGGGCGGTCGACGGCGGGTGGCAATCCGGCTAAGGTGTGCGCCCCGTATCACCTTGGCCGCTTCTGGCCTGAAACAGGAGCCTGAGCATGGACCTGCTGTTCCTTGGCACCTCCGCCGGGGTGCCCACCAAGGCACGCAATGTCAGCGCCACAGCCGTGATCGAAGCCAGCGGCAGCCACTGGTATCTGGTCGATTGCGGCGAAGGCACCCAGCACCGCCTGCTGCACACCCCGCTTTCGATCCGCGACCTGCGCGCGATCTTCATCA  >  NC_002947/4545414‑4545712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`