BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,099,202	G→T	5.4%	intergenic (+223/+69)	PP_mr66 → / ← acsA‑I	P6/acetyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,099,202	0	G	T	5.4%	346.5 / 5.2	103	intergenic (+223/+69)	PP_mr66/acsA‑I	P6/acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand): ref base G (70/27); new base T (3/3); total (73/30)
Fisher's exact test for biased strand distribution p-value = 3.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 106 total aligned reads displayed.

ACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATC  >  NC_002947/5099055‑5099351

acaAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGAc                                                                                                                                                      >  2:666736/1‑150 (MQ=255)

  aaaaTGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAg                                                                                                                                                    <  2:4263574/150‑1 (MQ=255)

  aaaaTGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAg                                                                                                                                                    <  1:2938071/150‑1 (MQ=255)

    aaTGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCt                                                                                                                                                  <  1:4429489/150‑1 (MQ=255)

    aaTGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCt                                                                                                                                                  <  1:4429641/150‑1 (MQ=255)

        cTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGc                                                                                                                                             <  1:3810678/150‑1 (MQ=255)

        cTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGc                                                                                                                                             <  2:2555191/150‑1 (MQ=255)

        cTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGc                                                                                                                                             >  2:1342947/1‑150 (MQ=255)

         tGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTTCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGcc                                                                                                                                            >  1:2418374/1‑150 (MQ=255)

          gACTCCGATGCACCGACCCGGGCCGGAATTGGTTCCTGGCGCCCCGAACCGGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAACGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCt                                                                                                                                           <  2:3971337/150‑1 (MQ=255)

             tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGaa                                                                                                                                        <  2:2974950/150‑1 (MQ=255)

             tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGaa                                                                                                                                        <  1:1052507/150‑1 (MQ=255)

             tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCG‑TAGCCTGaa                                                                                                                                        >  2:2935683/1‑150 (MQ=255)

             tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCG‑TAGCCTGaa                                                                                                                                        >  1:1098971/1‑150 (MQ=255)

                gATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCGGAACTg                                                                                                                                     >  2:2690628/1‑150 (MQ=255)

                gATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCCCGCCTGCGGGAGGTCCGGGGCCCGCA‑TAGCCAGAAcgg                                                                                                                                     >  1:3499278/1‑148 (MQ=255)

                gATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTTCCCGCCAAAGCGTGAGGTCAGGGGGCAGCG‑TAGCCGGAAATg                                                                                                                                     >  1:3499382/1‑150 (MQ=255)

                 aTGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGa                                                                                                                                    <  1:4322287/150‑1 (MQ=255)

                 aTGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGa                                                                                                                                    <  1:2277226/150‑1 (MQ=255)

                  tGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAc                                                                                                                                   <  2:3125290/150‑1 (MQ=255)

                  tGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAc                                                                                                                                   <  1:716507/150‑1 (MQ=255)

                   gCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTGGCCGCGAAAGCGGGCGGTCAGGGGGCAGCG‑TAGCCGGAAcataac                                                                                                                                  >  1:1998430/1‑145 (MQ=255)

                     aCCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCACGGGACAGCT‑TAGCCGGAACTGACGCa                                                                                                                                >  2:2521189/1‑150 (MQ=255)

                     aCCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCCCCAAATCGTGAGGTAAGGGGACAGCT‑TAGCCAGAACAGACGCa                                                                                                                                >  1:2889969/1‑150 (MQ=255)

                     aCCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TTGCCTGAACTGACGCa                                                                                                                                >  2:4371589/1‑150 (MQ=255)

                     aCCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTGCCCGCGAATGCGTGAGGTATGGGGTCAGGT‑TTACCTGTACAGAAGCa                                                                                                                                >  1:3939740/1‑150 (MQ=255)

                     aCCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGGAACAATGCAAAAGCGGGCTTGCCCGCGCATGCGTGAGGTCGGGGGACAGCG‑TAGCCGGGACTGACGcg                                                                                                                                >  2:2635662/1‑149 (MQ=255)

                      ccGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCAt                                                                                                                               <  2:3033259/150‑1 (MQ=255)

                      ccGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCAt                                                                                                                               <  2:3033331/150‑1 (MQ=255)

                       cGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGCATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCagt                                                                                                                              >  2:3285381/1‑148 (MQ=255)

                       cGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCAtt                                                                                                                              >  2:3285375/1‑150 (MQ=255)

                          aaCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCA‑TAGCCTGAACTGACGCATTagc                                                                                                                           >  1:1815962/1‑150 (MQ=255)

                           aCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTcgcg                                                                                                                          >  2:3850138/1‑150 (MQ=255)

                            cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                         <  2:4226408/150‑1 (MQ=255)

                            cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                         <  1:3111256/150‑1 (MQ=255)

                             gggCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCggg                                                                                                                        <  2:2613711/150‑1 (MQ=255)

                              ggCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTTCCCGCGCATGCGGGAGGTCCGGGGACAGCT‑TAGCCTGAACTGACGCATTAGCGGGc                                                                                                                       >  1:4213106/1‑150 (MQ=255)

                                ccGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGGCAGCT‑TAGCCGGGACTGTCGCATTCGCGGGcca                                                                                                                     >  1:4005280/1‑148 (MQ=255)

                                 cGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGcc                                                                                                                                            <  1:177730/126‑1 (MQ=255)

                                 cGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGcc                                                                                                                                            >  2:177730/1‑126 (MQ=255)

                                 cGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCGAg                                                                                                                    >  2:3475970/1‑150 (MQ=255)

                                  ggAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGc                                                                                                                   <  1:4343698/150‑1 (MQ=255)

                                    aaTTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGccc                                                                                                                 >  2:1698864/1‑150 (MQ=255)

                                        gTTTCCTGGCGCCCCGAACCGGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGCAAGCCCGCtt                                                                                                             <  2:3152516/150‑2 (MQ=18)

                                         tttCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTGAGGGGACAGCT‑TAGCCTGAACGGACGCATTCGCGGGCAAGCCCGCGcc                                                                                                            >  1:302277/1‑150 (MQ=255)

                                         tttCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCTTGCCCGcgaa                                                                                                            <  1:3927725/150‑4 (MQ=255)

                                          ttCCTGGCGCCCCGAACCTGGGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAAGGCAAACGCGGGCTTGCCCGCTAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGCAAGCCCGCtttt                                                                                                           <  2:512336/150‑4 (MQ=17)

                                           tCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGAGTGAGGTGAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTg                                                                                                          >  2:4312093/1‑150 (MQ=255)

                                            tcaccggcCCCGCACAGGTTCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGc                                                                                                         >  2:282165/7‑150 (MQ=39)

                                              tGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAg                                                                                                       >  2:2112799/1‑150 (MQ=255)

                                                gcgcCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAggg                                                                                                     >  2:3297975/1‑150 (MQ=255)

                                                   ccccGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGt                                                                                                  >  2:3057568/1‑150 (MQ=255)

                                                    cccGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGCCAGCA‑TAGCCTGAACTGTCGCATTCGCGGGCCAGCCCGCTCCTGCCGGGGGtg                                                                                                 >  2:3585178/1‑149 (MQ=255)

                                                    cccGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGGCGCATTAGCGGGCAAGCCCGCTCCTGCAGGGGGTa                                                                                                 >  2:2141093/1‑150 (MQ=255)

                                                    cccGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTa                                                                                                 >  2:4463592/1‑150 (MQ=255)

                                                    cccGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTGCCCGCGAAAGCGGGGGGTCAGGGGACAGCG‑TAGCCGGAAATGACGCAATAGCGGGCACCCCCGCGCCGGCCgggggga                                                                                                 >  1:3624451/1‑148 (MQ=255)

                                                     ccGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTcgcg                                                                                                                          <  1:3613533/124‑1 (MQ=255)

                                                     ccGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTcgcg                                                                                                                          >  2:3613533/1‑124 (MQ=255)

                                                     ccGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGGGAGGTCAGGGGCCCGCG‑TAGCCGGAACTGACGCAGTCGCGGGCCAGCCCGCGCCGGCCGGGGGGAt                                                                                                >  2:3089726/1‑150 (MQ=255)

                                                         cccTGGGGGGGGCCGGTGACAGAACTGTACCCGATGATGGTCGGGTAACACGGCAACCGGGGGCTGGCCCGCGATTGGGTGTGGTCCGGGGACCGCT‑TCGCCTGAACTGCCGCCTGCGGGGGCAAGCCCGCTCCTGCAGGGGGTATTGcc                                                                                            <  2:1089208/149‑1 (MQ=255)

                                                                     ccGGTGACAGAACCGTACCCGTTGAGTGTCGGGTAACACTGCAAACGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTa                                                                                <  2:4433370/150‑1 (MQ=39)

                                                                      cGGTGACAGAACAGTAACCGATGATTGTCGGGTAACCATGCAAACGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TCGCCTGAACTGACGCATGCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTAc                                                                               <  2:2574373/150‑1 (MQ=255)

                                                                             aGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGGTTGGCCGGGTATGGGTTTGGTGATGGGTGATCT‑TTTGCTGTAATGTCGCATTCGCGGGGAAGCCCGCTGCTGGAGGGGGTATTGGGGGGTAGGGTTTTCGCCGAAg                                                                        >  2:1587581/1‑150 (MQ=255)

                                                                             aGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAAAGCGTGAGGTCAGGGGACAGCT‑TATCCTGAACTGACGCATTAGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAg                                                                        >  2:1269354/1‑150 (MQ=255)

                                                                                      aCCGATGATTGTCGGGTAACAATGCAAAAGCGGGCATGGCCGCGTAATCGTGTGGTGAGGGGACATCT‑TTGCCGGTGATGTCGCATTAGACGGCAAGAACGCACCTGCAGGGGGTATTGCCGGGAAGGGGTTACTACCAAGACACGTTCa                                                               >  2:1590741/1‑150 (MQ=255)

                                                                                                 tCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATTCGTGTGGTCAGGGGGCACCT‑TAGCCTGGACTGACGCAATCGCGGGCAAGCCCCCTCCTGCAGGGGGTATTTCCGGGAACGGTTTAAGCCCAAGCCACGTTCAACGCCGTGTgg                                                    >  1:1416448/1‑150 (MQ=255)

                                                                                                   gggTAACAATGCAAAAGCGGGCTTGCCCGCGAAAGCGTGGGGTGAGGGGACCGCT‑TAGCCTGAACGGACGCAATAGCGGGCAAGCCCGCTCCGGCAGGGGGTATTGGCGGGAAGGGTTTACGCCGGAGCCAGGTTCATAGCCGTTTGGGt                                                  >  1:4031300/1‑150 (MQ=255)

                                                                                                     gTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGGCCGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCCCGCCTGCCGGGGGGATTGCCGGGCAGGGGTTACGCCGAAGCCAGGGTCATCGCCGTGTGGGtgt                                                >  1:3333626/1‑148 (MQ=255)

                                                                                                     gTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTAt                                                >  2:2618931/1‑150 (MQ=255)

                                                                                                              cAAAAGCGGGCTTGCCCGCCAAAGCGTGAGGTCAGGGGCCACCG‑TTGCCGGGACTGACGCATTAGCGGGCAAGCCCCCTCCGGCACGGGGGATTGCCGGGACGGGTTTACGCCCAAGCCCGGGTCATCGCCATGTGGGTATAGGTAAGGt                                       >  2:2417960/1‑150 (MQ=255)

                                                                                                                aacgggggCTTGCCCGCTACTGCGTGTGTTCTGGGGACAGCT‑TCGCCTGAACTGACGCATTCGGGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGc                                     <  2:681919/145‑1 (MQ=255)

                                                                                                                    ggggCTGGCCCGCTATTGGGTGTGGCCGGGGTACTGTT‑TCGCCTCAACGCCCGCTTGCGGGGCCACGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTa                                 <  2:1998430/149‑1 (MQ=255)

                                                                                                                    cGGGCTTGCCCGCGAATGCGTGAGGTCCGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTa                                 >  1:1173471/1‑150 (MQ=255)

                                                                                                                     gggCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTAc                                >  1:4505534/1‑150 (MQ=255)

                                                                                                                       gCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACca                              >  1:1049412/1‑150 (MQ=255)

                                                                                                                       gCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACca                              >  1:2516328/1‑150 (MQ=255)

                                                                                                                            cccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACAccc                         >  1:3858199/1‑150 (MQ=255)

                                                                                                                            cccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTAATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACAccc                         >  1:775556/1‑150 (MQ=255)

                                                                                                                             ccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCg                        >  2:2886584/1‑150 (MQ=255)

                                                                                                                             ccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCg                        >  2:2775295/1‑150 (MQ=255)

                                                                                                                             ccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCg                        >  1:2755240/1‑150 (MQ=255)

                                                                                                                             ccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCg                        >  1:4064187/1‑150 (MQ=255)

                                                                                                                             ccGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCg                        >  2:2742753/1‑150 (MQ=21)

                                                                                                                             ccGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCg                        >  2:1133059/1‑150 (MQ=21)

                                                                                                                              cgcgAATGCGTGAGGTCCGGGGACAGCT‑TCGCCTGAACTGACGCATGCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCgg                       <  2:4507294/150‑1 (MQ=255)

                                                                                                                               gcgAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCggg                      >  2:3095308/1‑150 (MQ=255)

                                                                                                                               gcgAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCggg                      >  2:3140252/1‑150 (MQ=21)

                                                                                                                                   aTGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAg                  >  2:3711183/1‑150 (MQ=255)

                                                                                                                                      cGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCa               >  2:2833724/1‑150 (MQ=255)

                                                                                                                                       gTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCaa              >  1:508979/1‑150 (MQ=255)

                                                                                                                                       gTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCaa              >  1:4236809/1‑150 (MQ=255)

                                                                                                                                        tGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAg             >  1:901065/1‑150 (MQ=255)

                                                                                                                                        tGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAg             >  1:901041/1‑150 (MQ=255)

                                                                                                                                         gAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAgg            >  1:724834/1‑150 (MQ=255)

                                                                                                                                            gTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCg         >  2:45309/1‑150 (MQ=255)

                                                                                                                                             tCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCga        >  1:3943235/1‑150 (MQ=255)

                                                                                                                                               aGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAACCAAGGTCgaga      >  1:2322367/1‑150 (MQ=255)

                                                                                                                                                ggggACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGat     >  1:429435/1‑150 (MQ=255)

                                                                                                                                                 gggACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGata    >  1:3912406/1‑150 (MQ=255)

                                                                                                                                                   gACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTAGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATc  >  1:3801169/1‑150 (MQ=255)

ACAAAATGCTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATC  >  NC_002947/5099055‑5099351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`