BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,099,210	A→C	8.0%	intergenic (+231/+61)	PP_mr66 → / ← acsA‑I	P6/acetyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,099,210	0	A	C	8.0%	294.7 / 8.0	104	intergenic (+231/+61)	PP_mr66/acsA‑I	P6/acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand): ref base A (69/20); new base C (3/7); total (77/27)
Fisher's exact test for biased strand distribution p-value = 3.79e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 104 total aligned reads displayed.

CTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAG  >  NC_002947/5099063‑5099357

cTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGc                                                                                                                                                   <  2:2555191/150‑1 (MQ=255)

cTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGc                                                                                                                                                   <  1:3810678/150‑1 (MQ=255)

cTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGc                                                                                                                                                   >  2:1342947/1‑150 (MQ=255)

 tGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTTCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGcc                                                                                                                                                  >  1:2418374/1‑150 (MQ=255)

  gACTCCGATGCACCGACCCGGGCCGGAATTGGTTCCTGGCGCCCCGAACCGGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAACGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCt                                                                                                                                                 <  2:3971337/150‑1 (MQ=255)

     tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGaa                                                                                                                                              <  1:1052507/150‑1 (MQ=255)

     tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGaa                                                                                                                                              <  2:2974950/150‑1 (MQ=255)

     tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCG‑TAGCCTGaa                                                                                                                                              >  1:1098971/1‑150 (MQ=255)

     tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCG‑TAGCCTGaa                                                                                                                                              >  2:2935683/1‑150 (MQ=255)

     tCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTGCCCGCGAATTCGTGCGGGCAGGGGACAGGG‑TAGCCTGaa                                                                                                                                              >  1:4241837/1‑150 (MQ=255)

        gATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCGGAACTg                                                                                                                                           >  2:2690628/1‑150 (MQ=255)

        gATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCCCGCCTGCGGGAGGTCCGGGGCCCGCA‑TAGCCAGAAcgg                                                                                                                                           >  1:3499278/1‑148 (MQ=255)

        gATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTTCCCGCCAAAGCGTGAGGTCAGGGGGCAGCG‑TAGCCGGAAATg                                                                                                                                           >  1:3499382/1‑150 (MQ=255)

         aTGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGa                                                                                                                                          <  1:4322287/150‑1 (MQ=255)

         aTGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGa                                                                                                                                          <  1:2277226/150‑1 (MQ=255)

          tGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAc                                                                                                                                         <  2:3125290/150‑1 (MQ=255)

          tGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAc                                                                                                                                         <  1:716507/150‑1 (MQ=255)

           gCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTGGCCGCGAAAGCGGGCGGTCAGGGGGCAGCG‑TAGCCGGAAcataac                                                                                                                                        >  1:1998430/1‑145 (MQ=255)

             aCCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCACGGGACAGCT‑TAGCCGGAACTGACGCa                                                                                                                                      >  2:2521189/1‑150 (MQ=255)

             aCCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCCCCAAATCGTGAGGTAAGGGGACAGCT‑TAGCCAGAACAGACGCa                                                                                                                                      >  1:2889969/1‑150 (MQ=255)

             aCCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TTGCCTGAACTGACGCa                                                                                                                                      >  2:4371589/1‑150 (MQ=255)

             aCCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTGCCCGCGAATGCGTGAGGTATGGGGTCAGGT‑TTACCTGTACAGAAGCa                                                                                                                                      >  1:3939740/1‑150 (MQ=255)

             aCCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGGAACAATGCAAAAGCGGGCTTGCCCGCGCATGCGTGAGGTCGGGGGACAGCG‑TAGCCGGGACTGACGcg                                                                                                                                      >  2:2635662/1‑149 (MQ=255)

              ccGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCAt                                                                                                                                     <  2:3033259/150‑1 (MQ=255)

              ccGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCAt                                                                                                                                     <  2:3033331/150‑1 (MQ=255)

               cGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGCATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCagt                                                                                                                                    >  2:3285381/1‑148 (MQ=255)

               cGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCAtt                                                                                                                                    >  2:3285375/1‑150 (MQ=255)

                  aaCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCA‑TAGCCTGAACTGACGCATTagc                                                                                                                                 >  1:1815962/1‑150 (MQ=255)

                   aCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTcgcg                                                                                                                                >  2:3850138/1‑150 (MQ=255)

                    cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                               <  1:3111256/150‑1 (MQ=255)

                    cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCgg                                                                                                                               <  2:4226408/150‑1 (MQ=255)

                     gggCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCggg                                                                                                                              <  2:2613711/150‑1 (MQ=255)

                      ggCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTTCCCGCGCATGCGGGAGGTCCGGGGACAGCT‑TAGCCTGAACTGACGCATTAGCGGGc                                                                                                                             >  1:4213106/1‑150 (MQ=255)

                        ccGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGGCAGCT‑TAGCCGGGACTGTCGCATTCGCGGGcca                                                                                                                           >  1:4005280/1‑148 (MQ=255)

                         cGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGcc                                                                                                                                                  <  1:177730/126‑1 (MQ=255)

                         cGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGcc                                                                                                                                                  >  2:177730/1‑126 (MQ=255)

                         cGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCGAg                                                                                                                          >  2:3475970/1‑150 (MQ=255)

                          ggAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGc                                                                                                                         <  1:4343698/150‑1 (MQ=255)

                            aaTTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGccc                                                                                                                       >  2:1698864/1‑150 (MQ=255)

                                gTTTCCTGGCGCCCCGAACCGGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGCAAGCCCGCtt                                                                                                                   <  2:3152516/150‑2 (MQ=18)

                                 tttCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTGAGGGGACAGCT‑TAGCCTGAACGGACGCATTCGCGGGCAAGCCCGCGcc                                                                                                                  >  1:302277/1‑150 (MQ=255)

                                 tttCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCTTGCCCGcgaa                                                                                                                  <  1:3927725/150‑4 (MQ=255)

                                  ttCCTGGCGCCCCGAACCTGGGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAAGGCAAACGCGGGCTTGCCCGCTAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGCAAGCCCGCtttt                                                                                                                 <  2:512336/150‑4 (MQ=17)

                                   tCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGAGTGAGGTGAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTg                                                                                                                >  2:4312093/1‑150 (MQ=255)

                                    tcaccggcCCCGCACAGGTTCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGc                                                                                                               >  2:282165/7‑150 (MQ=39)

                                      tGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAg                                                                                                             >  2:2112799/1‑150 (MQ=255)

                                        gcgcCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAggg                                                                                                           >  2:3297975/1‑150 (MQ=255)

                                        gcgcCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAggg                                                                                                           >  2:1460867/1‑150 (MQ=255)

                                           ccccGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGt                                                                                                        >  2:3057568/1‑150 (MQ=255)

                                            cccGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGCCAGCA‑TAGCCTGAACTGTCGCATTCGCGGGCCAGCCCGCTCCTGCCGGGGGtg                                                                                                       >  2:3585178/1‑149 (MQ=255)

                                            cccGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGGCGCATTAGCGGGCAAGCCCGCTCCTGCAGGGGGTa                                                                                                       >  2:2141093/1‑150 (MQ=255)

                                            cccGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTa                                                                                                       >  2:4463592/1‑150 (MQ=255)

                                            cccGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCGTGCCCGCGAAAGCGGGGGGTCAGGGGACAGCG‑TAGCCGGAAATGACGCAATAGCGGGCACCCCCGCGCCGGCCgggggga                                                                                                       >  1:3624451/1‑148 (MQ=255)

                                             ccGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTcgcg                                                                                                                                <  1:3613533/124‑1 (MQ=255)

                                             ccGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTcgcg                                                                                                                                >  2:3613533/1‑124 (MQ=255)

                                                 cccTGGGGGGGGCCGGTGACAGAACTGTACCCGATGATGGTCGGGTAACACGGCAACCGGGGGCTGGCCCGCGATTGGGTGTGGTCCGGGGACCGCT‑TCGCCTGAACTGCCGCCTGCGGGGGCAAGCCCGCTCCTGCAGGGGGTATTGcc                                                                                                  <  2:1089208/149‑1 (MQ=255)

                                                             ccGGTGACAGAACCGTACCCGTTGAGTGTCGGGTAACACTGCAAACGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTa                                                                                      <  2:4433370/150‑1 (MQ=39)

                                                              cGGTGACAGAACAGTAACCGATGATTGTCGGGTAACCATGCAAACGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TCGCCTGAACTGACGCATGCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTAc                                                                                     <  2:2574373/150‑1 (MQ=255)

                                                                   aCAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGCATGCGTGAGGTCCGGGGACAGCG‑TAGCCTGTACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGa                                                                                >  2:3525830/1‑150 (MQ=255)

                                                                     aGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAAAGCGTGAGGTCAGGGGACAGCT‑TATCCTGAACTGACGCATTAGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAg                                                                              >  2:1269354/1‑150 (MQ=255)

                                                                              aCCGATGATTGTCGGGTAACAATGCAAAAGCGGGCATGGCCGCGTAATCGTGTGGTGAGGGGACATCT‑TTGCCGGTGATGTCGCATTAGACGGCAAGAACGCACCTGCAGGGGGTATTGCCGGGAAGGGGTTACTACCAAGACACGTTCa                                                                     >  2:1590741/1‑150 (MQ=255)

                                                                               ccGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTTCCCCCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCGGAACTGACGCATTCGCGGGCCAGCCCGCTCCAGCAGGGGGGATTTCCGGGGAGGGTTTACGCCGAAGCCAGGTTCaa                                                                    >  1:326159/1‑149 (MQ=255)

                                                                                         tCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATTCGTGTGGTCAGGGGGCACCT‑TAGCCTGGACTGACGCAATCGCGGGCAAGCCCCCTCCTGCAGGGGGTATTTCCGGGAACGGTTTAAGCCCAAGCCACGTTCAACGCCGTGTgg                                                          >  1:1416448/1‑150 (MQ=255)

                                                                                           gggTAACAATGCAAAAGCGGGCTTGCCCGCGAAAGCGTGGGGTGAGGGGACCGCT‑TAGCCTGAACGGACGCAATAGCGGGCAAGCCCGCTCCGGCAGGGGGTATTGGCGGGAAGGGTTTACGCCGGAGCCAGGTTCATAGCCGTTTGGGt                                                        >  1:4031300/1‑150 (MQ=255)

                                                                                             gTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGGCCGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCCCGCCTGCCGGGGGGATTGCCGGGCAGGGGTTACGCCGAAGCCAGGGTCATCGCCGTGTGGGtgt                                                      >  1:3333626/1‑148 (MQ=255)

                                                                                             gTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTAt                                                      >  2:2618931/1‑150 (MQ=255)

                                                                                                  aaTGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCGGAACTGACGCATTAGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGCAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATc                                                 >  2:280507/1‑150 (MQ=255)

                                                                                                      cAAAAGCGGGCTTGCCCGCCAAAGCGTGAGGTCAGGGGCCACCG‑TTGCCGGGACTGACGCATTAGCGGGCAAGCCCCCTCCGGCACGGGGGATTGCCGGGACGGGTTTACGCCCAAGCCCGGGTCATCGCCATGTGGGTATAGGTAAGGt                                             >  2:2417960/1‑150 (MQ=255)

                                                                                                        aacgggggCTTGCCCGCTACTGCGTGTGTTCTGGGGACAGCT‑TCGCCTGAACTGACGCATTCGGGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGc                                           <  2:681919/145‑1 (MQ=255)

                                                                                                            ggggCTGGCCCGCTATTGGGTGTGGCCGGGGTACTGTT‑TCGCCTCAACGCCCGCTTGCGGGGCCACGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTa                                       <  2:1998430/149‑1 (MQ=255)

                                                                                                            cGGGCTTGCCCGCGAATGCGTGAGGTCCGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTa                                       >  1:1173471/1‑150 (MQ=255)

                                                                                                             gggCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTAc                                      >  1:4505534/1‑150 (MQ=255)

                                                                                                               gCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACca                                    >  1:2516328/1‑150 (MQ=255)

                                                                                                               gCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACca                                    >  1:1049412/1‑150 (MQ=255)

                                                                                                                    cccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTAATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACAccc                               >  1:775556/1‑150 (MQ=255)

                                                                                                                     ccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCg                              >  2:2886584/1‑150 (MQ=255)

                                                                                                                     ccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCg                              >  1:2755240/1‑150 (MQ=255)

                                                                                                                     ccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCg                              >  2:2775295/1‑150 (MQ=255)

                                                                                                                     ccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCg                              >  1:4064187/1‑150 (MQ=255)

                                                                                                                     ccGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCg                              >  2:1133059/1‑150 (MQ=21)

                                                                                                                     ccGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCg                              >  2:2742753/1‑150 (MQ=21)

                                                                                                                      cgcgAATGCGTGAGGTCCGGGGACAGCT‑TCGCCTGAACTGACGCATGCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCgg                             <  2:4507294/150‑1 (MQ=255)

                                                                                                                       gcgAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCggg                            >  2:3095308/1‑150 (MQ=255)

                                                                                                                       gcgAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCggg                            >  2:3140252/1‑150 (MQ=21)

                                                                                                                           aTGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAg                        >  2:3711183/1‑150 (MQ=255)

                                                                                                                              cGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCa                     >  2:2833724/1‑150 (MQ=255)

                                                                                                                               gTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCaa                    >  1:4236809/1‑150 (MQ=255)

                                                                                                                               gTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCaa                    >  1:508979/1‑150 (MQ=255)

                                                                                                                                tGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAg                   >  1:901065/1‑150 (MQ=255)

                                                                                                                                tGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAg                   >  1:901041/1‑150 (MQ=255)

                                                                                                                                 gAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAgg                  >  1:724834/1‑150 (MQ=255)

                                                                                                                                    gTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCg               >  2:45309/1‑150 (MQ=255)

                                                                                                                                     tctggttaCAGCT‑TAGCCTGAACTGACGATTTCGGGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCga              <  2:4241837/143‑1 (MQ=255)

                                                                                                                                     tCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCga              >  1:3943235/1‑150 (MQ=255)

                                                                                                                                       aGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAACCAAGGTCgaga            >  1:2322367/1‑150 (MQ=255)

                                                                                                                                        ggggACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGat           >  1:429435/1‑150 (MQ=255)

                                                                                                                                         gggACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGata          >  1:3912406/1‑150 (MQ=255)

                                                                                                                                           gACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTAGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATc        >  1:3801169/1‑150 (MQ=255)

                                                                                                                                                 t‑tAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAg  >  2:3210658/1‑150 (MQ=255)

                                                                                                                                                 t‑tAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAg  >  1:2415889/1‑150 (MQ=255)

CTGACTCCGATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAG  >  NC_002947/5099063‑5099357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`