| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,903,731 | 0 | G | C | 54.5% | 2.9 / 12.8 | 11 | intergenic (+214/‑57) | PP_1703/nirB | assimilatory nitrate reductase/sulfite reductase/nitrite reductase large subunit |
| Reads supporting (aligned to +/- strand): ref base G (3/2); new base C (0/6); total (3/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.06e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGAGGCCGACACAGGCAACCCAGGCAACCCACGCAACCCGATTGGCACGCTCCCTGCTTAACCTCGGATACAACAAAGCCCACTGATCAACGGCGATCAACCGGGCCCCCTACCGTGATGAATACAGGCAAAGGCGCCTGGAGCTTCGGCTCCAGGC‑GCTTTTTTTTTGATCGAGGATCGGCTTATGAAGGCAACAGCGAGCAGCGGGCAACGAGAGCGACTGGTCATCGTC > NC_002947/1903604‑1903835 | agagGCCGACACAGGCAACCCAGGCAACCCACGCAACCCGATTGGCACGCTCCCTGCTTAACCTCGGATACAACAAAGCCCACTGATCAACGGCGATCAACCGGGCCCCCTACCGTGATGAATACAGGCAAAGgcg > 1:543827/1‑136 (MQ=255)agagGCCGACACAGGCAACCCAGGCAACCCACGCAACCCGATTGGCACGCTCCCTGCTTAACCTCGGATACAACAAAGCCCACTGATCAACGGCGATCAACCGGGCCCCCTACCGTGATGAATACAGGCAAAGgcg > 1:94785/1‑136 (MQ=255) agGCCGACACAGGCAACCCAGGCAACCCACGCAACCAGATTGGCACGCTCCCTGCTTAACCTCGGATACAACAAAGCCCACTGATCAACGGCGATCAACCGGGCCCCCTACCGTGATGAATACAGGCAAAGGCGcc < 2:321020/136‑1 (MQ=255) aaCCCGATTGGCACGCTCCCTGCTTAACCTCGGATACAACAAAGCCCACTGATCAACGGCGATCAACCGGGCCCCCTACCGTGATGAATACAGGCAAAGGCGCCAGGAGGATAGGCGCCACGCGGCgttttttttt > 1:505635/1‑135 (MQ=255) ctaTTGGCACGCACCCTGATTAACCTCGTAAACAACAACCCCCACTTACCACCGGCGACCAACCGGCCCCCCTCCCGTCATCAAAACCGCAAACGCCGCCTGGAGCTGCGCCTCCTGGC‑GCTTTTTTTTTGATCGa < 2:478839/134‑1 (MQ=255) tGGCACGCTCCCTGCTTAACGTCGGATACAACAACGCCCACTGATCAAGGGCGTACAACGGGGCCCCCTACCGTTTTTATTACAGCCATAGGCGCCTGGTGCTTCGGCTCCTGGC‑GCTTTTTTTTTGATCGAGGAt < 2:365632/136‑1 (MQ=255) cACGCCCCCTGCTTAACCTCGGAAACAACAACGCCCACTGATCACCGGCGATCACCCGGGCCCCCTACCGTGATTAATACAGCAAAAGGCGCCTGGCGCTTCGGCTCCTGGC‑GCTTTTTTTTTGATCGAGGATCgg < 1:186808/136‑1 (MQ=255) cGCTCCCTGCTTAACCTCGTATACAACAAAGCCCACTGAACAACGGCGAACAACCGGCCCCCCTACCTTCTTTAAAACCGCCAAAGGCGCCGGTAGCTTCGGCTCCAGGC‑GCTTTTTTTTTGATCGAGGATCGGCt < 2:53011/136‑1 (MQ=255) aTACAACAAAGCCCACTGATCAACGGCGATCAACCGGGCCCCCTACCGTGATGAATACAGGCAAAGGCGCCAGGAGCTTCGGCTCCAGGC‑GCTTTTTTTTTGATCGAGGATCGGCTTATGAAGGCAACAGCGagca < 1:92996/136‑1 (MQ=255) acaacaACCCCCCCTCATCAACGCCGAACAACCGGCCCCCCACCCGTTATTAAAACAGCCAACGGCGCCTGCAGCTTCGGCGCCCGGC‑GCTTTTTTTTTGATCGAGGATCGGCTTATGAAGGCAACAGCGagcagc < 2:436347/136‑1 (MQ=255) acacccGGCCCCCCTCCCGTTTTTAAAACCGCCAACGGCGCGGGGAGGTGCGGCCCCTGGC‑GCTTTTTTTTTGATCGAGGATCGGCTTATGAAGGCAACAGCGAGCAGCGGGCAACGAGAGCGACTGGTCAtcgtc < 1:435111/132‑1 (MQ=255) | AGAGGCCGACACAGGCAACCCAGGCAACCCACGCAACCCGATTGGCACGCTCCCTGCTTAACCTCGGATACAACAAAGCCCACTGATCAACGGCGATCAACCGGGCCCCCTACCGTGATGAATACAGGCAAAGGCGCCTGGAGCTTCGGCTCCAGGC‑GCTTTTTTTTTGATCGAGGATCGGCTTATGAAGGCAACAGCGAGCAGCGGGCAACGAGAGCGACTGGTCATCGTC > NC_002947/1903604‑1903835 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |