| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,990,787 | 0 | G | A | 61.3% | 18.9 / 30.5 | 31 | intergenic (+103/‑443) | kefB‑III/PP_mr64 | glutathione‑regulated potassium/H+ antiporter/C4 ncRNA |
| Reads supporting (aligned to +/- strand): ref base G (2/10); new base A (10/9); total (12/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.52e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GAGCGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCAC > NC_002947/5990655‑5990874 | gtgggtggtgttcgccgggggtttttcagcgccggggaggtggtcgccgggagtgtttcagcgcctgggagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTC‑GGCcagtccc < 2:578913‑M2/48‑4 (MQ=255)gtgggaggtttggggcgggggtttttatgcgcctgggaggtgttcgcggggagtgtttctgcgcctgggagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTC‑GGCcagtccc < 2:232922‑M2/48‑4 (MQ=255)gtgggaggtgttcgccgggggattttcagcgcctgggaggtgttcgccgggagagtttcagcgcctgggagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTC‑GGCcagtccc < 1:176875‑M2/48‑4 (MQ=255)ctggtaggtcttggccggggtattttcttggcctgggaggtgttggccgggagtgtttcagcgcctgggagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTC‑GGCcagtccc < 2:543578‑M2/48‑4 (MQ=255)ctgggtggtgttcgccgggggattttcagcgcctgggaggtgttcgccgggagtgtttcagcgcctggtagatcgagcgccgcccgcgGGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTC‑GGCcagtccc < 1:107774‑M2/48‑4 (MQ=255)ctgggaggtgttcgccgggggattttcagcgcctgggaggtgttcgccgggagagtttcagcgcctgggagatcgagcgccgcccgcgCGGCGCTTCGTGGGTAAACCCGCTCCTACGTTTGTTTC‑GGCcagtccc < 1:440044‑M2/48‑4 (MQ=255)ctgggaggtgttcgccgggggattttcagcgcctgggaggtgttcgccgggagagtttcagcgcctgggagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTC‑GGCcagtccc < 2:362745‑M2/48‑4 (MQ=255)ctgggaggtgttcgccgggggattttcagcgcctgggaggtgttcgccgggagagtttcagcgcctgggagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTC‑GGCcagtccc < 1:448708‑M2/48‑4 (MQ=255)ctgggagggtttcgccgggggattttcagcgcctgggaggtgttcgcggggagagtttcagcgcctgggagatcaagcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTC‑GGCcagtccc < 1:322031‑M2/48‑4 (MQ=255) tttgaggtgtttgccggaggattttcagcgcctgggaggtgttcgccgggagagtttcagcgcctgggagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTAt < 2:368063‑M2/49‑1 (MQ=255) tgccgggggattttcagcgcctgggaggtgttcgccgggagagtttcagcgcctgggagatcgagcgccgcccgcgCGGCGCGTAGCGGGTAAAACCGCGCCTAAGTTTGTTTAGGGGCAATAAATACTGCGGgat > 1:422107‑M2/77‑135 (MQ=255) cgccgggggattttcagcgcctgggaggtgttcgccgggagagtttcagcgcctgggagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCAACCTTTGTTTAGGGCCAATTATTACTGCGGgat > 1:156067‑M2/77‑135 (MQ=255) ccTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGgc < 1:69658/136‑1 (MQ=255) aggtgtttgccggaggattttcagcgcctgtgagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTAt < 2:313697‑M2/49‑1 (MQ=255) aggtgtttgccggaggattttcagcgcctgtgagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTAt > 1:313697‑M2/52‑100 (MQ=255) gccgggagagtttcagcgcctgtgagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTC‑GGCcagtccc > 1:216080‑M2/44‑88 (MQ=255) gccgggagagtttcagcgcctgtgagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTC‑GGCcagtccc < 2:216080‑M2/48‑4 (MQ=255) cctgtgagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTC‑GGCcagtccc > 2:132944‑M2/26‑70 (MQ=255) cctgtgagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTC‑GGCcagtccc < 1:132944‑M2/48‑4 (MQ=255) cctgggagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAattatt > 1:310996‑M2/26‑75 (MQ=255) cctgggagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAattatt < 2:310996‑M2/50‑1 (MQ=255) cctgggagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTAt > 1:339777‑M2/26‑74 (MQ=255) cctgggagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTAt > 1:33816‑M2/26‑74 (MQ=255) cctgggagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTAt < 2:339777‑M2/49‑1 (MQ=255) cctgggagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTAt < 2:33816‑M2/49‑1 (MQ=255) cctgggagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTAt < 1:287667‑M2/49‑1 (MQ=255) cctgggagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTAt < 1:281655‑M2/49‑1 (MQ=255) cctgggagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTAt > 1:16694‑M2/26‑74 (MQ=255) cctgggagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTAt > 2:134380‑M2/26‑74 (MQ=255) cctgggagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTAt > 2:287667‑M2/26‑74 (MQ=255) cctgggagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTAt > 2:281655‑M2/26‑74 (MQ=255) cctgggagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTAt < 2:16694‑M2/49‑1 (MQ=255) cctgggagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTAt < 1:134380‑M2/49‑1 (MQ=255) cctgggagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTc < 2:58560‑M2/51‑1 (MQ=255) cctgggagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTc > 1:58560‑M2/26‑76 (MQ=255) tttcgagcgccgcccgcgGGGCGCTTCGCGGGTAATCCCGCTCCTACGTTTGTTTCTGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTcg < 1:233753‑M2/118‑1 (MQ=255) tagcgcgcgccgcccgcgGGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTcg < 1:516531‑M2/118‑1 (MQ=255) gatggtgcgcgggccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTAGGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTcg < 2:136287‑M2/118‑1 (MQ=255) gatcgagcgccgcccgcgCGGGGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTcg < 1:246252‑M2/118‑1 (MQ=255) gatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTcg < 2:133530‑M2/118‑1 (MQ=255) gccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGcc < 1:245434‑M2/126‑1 (MQ=255) cgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTcg < 2:148935‑M2/118‑1 (MQ=255) cgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTcg > 1:148935‑M2/5‑122 (MQ=255) cgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGcac < 1:528302‑M2/132‑1 (MQ=255) gcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGtt > 1:576069‑M2/4‑50 (MQ=255) gcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGtt < 2:576069‑M2/47‑1 (MQ=255) | GAGCGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCAC > NC_002947/5990655‑5990874 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |