Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,821,017	Δ1 bp	100%	coding (738/1422 nt)	PP_1624 →	group II intron‑encoding maturase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,821,017	0	G	.	100.0%	42.2 / NA	10	coding (738/1422 nt)	PP_1624	group II intron‑encoding maturase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (10/0);  total (10/0)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GAGGGGACGCCGCAAGGCGGCCCGCTCTCGCCGTTGCTGTCGAATATCCTGCTCGACGAACTCGACCGCGAGCTGGAGCGGCGGGGCCATCGCTTCGTACGTTATGCCGATGATGCGAACATCTATGTACGCAGTCCCCGGGCCGGCGAACGGGT  >  NC_002947/1820994‑1821148                        |                                                                                                                                    gaggGGACGCCGCAAGGCGGCCC‑CTCCTGCCGTgggctgacgacaccgcccgacatgatcccggcttcaaggtagcggcgaataagcctgagtacgcacttgtctttgatccgacgttcgatgcaggccatgaggatgtcgtgattgacc      >  2:614740‑M1/1‑33 (MQ=255) gacgggacgCCGCAACGCGGCCC‑CTCCTGCCGAgtgctgacctcaccgccccacatgatcccggcttcaaggtagcggcgaaaaagcctgagtacgcacttgtctttgatccgacgttcgatgcaggccatgaggatgtcgtgattgacc      >  2:2320542‑M1/4‑33 (MQ=255)   ggggACGCCGCAAGGCGGCCC‑CTCCTGCCGTgggctgacgacaccgcccgacatgatcccggcttcaaggtagcggcgaataagcctgagtacgcacttgtctttgatccgacgttcgatgcaggccatgaggatgtcgtgattgacccg    >  1:6339205‑M1/1‑31 (MQ=255)    gggACGCCGCAAGGCGGCCC‑CTCCTGCCGTgggctgacgacaccgcccgacatgatcccggcttcaaggtagcggcgaataagcctgagtacgcacttgtctttgatccgacgttcgatgcaggccatgaggatgtcgtgattgacccga   >  1:2568440‑M1/1‑30 (MQ=255)    gggACGCCGCAAGGCGGCCC‑CTCCTGCCGTgggctgacgacaccgcccgacatgatcccggcttcaaggtagcggcgaataagcctgagtacgcacttgtctttgatccgacgttcgatgcaggccatgaggatgtcgtgattgacccga   >  2:1423101‑M1/1‑30 (MQ=255)    gggACGCCGCAAGGCGGCCC‑CTCCTGCCGTgggctgacgacaccgcccgacatgatcccggcttcaaggtagcggcgaataagcctgagtacgcacttgtctttgatccgacgttcgatgcaggccatgaggatgtcgtgattgacccga   >  2:1470000‑M1/1‑30 (MQ=255)    gggACGCCGCAAGGCGGCCC‑CTCCTGCCGTgggctgacgacaccgcccgacatgatcccggcttcaaggtagcggcgaataagcctgagtacgcacttgtctttgatccgacgttcgatgcaggccatgaggatgtcgtgattgacccga   >  2:5523727‑M1/1‑30 (MQ=255)    gggACGCCGCAAGGCGGCCC‑CTCCTGCCGTgggctgacgacaccgcccgacatgatcccggcttcaaggtagcggcgaataagcctgagtacgcacttgtctttgatccgacgttcgatgcaggccatgaggatgtcgtgattgacccga   >  2:7252672‑M1/1‑30 (MQ=255)    gggACGCCGCAAGGCGGCCC‑CTCCTGCCGTgggctgacgacaccgcccgacatgatcccggcttcaaggtagcggcgaataagcctgagtacgcacttgtctttgatccgacgttcgatgcaggccatgaggatgtcgtgattgacccga   >  2:7252765‑M1/1‑30 (MQ=255)     ggACGCCGCAAGGCGGCCC‑CTCCTGCCGTgggctgacgacaccgcccgacatgatcccggcttcaaggtagcggcgaataagcctgagtacgcacttgtctttgatccgacgttcgatgcaggccatgaggatgtcgtgattgacccgat  >  2:9060138‑M1/1‑29 (MQ=255)                        |                                                                                                                                    GAGGGGACGCCGCAAGGCGGCCCGCTCTCGCCGTTGCTGTCGAATATCCTGCTCGACGAACTCGACCGCGAGCTGGAGCGGCGGGGCCATCGCTTCGTACGTTATGCCGATGATGCGAACATCTATGTACGCAGTCCCCGGGCCGGCGAACGGGT  >  NC_002947/1820994‑1821148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑