BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,316,697	Δ1 bp	5.5%	coding (933/1209 nt)	aspC →	aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,316,697	0	A	.	5.5%	1063.1 / 50.1	310	coding (933/1209 nt)	aspC	aminotransferase
Reads supporting (aligned to +/- strand): ref base A (128/164); new base . (7/10); total (135/175)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 320 total aligned reads displayed.

GGCAAGCTCAGCCTGCTGCATGAAAGTGGGCAGCCGCGTATTTTACTCGATGCCGTGACCTGCATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCAGGGACTTTTCGCAATACCTGGCCACTGAGCTGGGTATCCTGACGATTCCCTATCA  >  NC_002947/4316551‑4316846

 gCAAGCTCAGCCTGCTGCATGAAAGTGGGCAGTCGCGTATTTTACTCGATGCCGTGACCTGCATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGTCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATg                                                                                                                                                    >  1:6034141/1‑150 (MQ=255)

      cTCAGCCTGCTGCATGAAAGTGGGCAGCCGCGTATTTTACTCGATGCCGTGACCTGCATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTg                                                                                                                                              <  2:7886760/150‑1 (MQ=255)

       tCAGCCTGCTGCATGAAAGTGGGCAGCCGCGTATTTTACTCGATGCCGTGACCTGCATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTgg                                                                                                                                             >  2:3723876/1‑150 (MQ=255)

        cAGCCTGCTGCATGAACGTGGGCAGCCGCGTATTTTACTCGCTGCCGTGACCTGCATTCTCGACGACCGGGGCGTCGCCGAACGCCTTTGCCAGGGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCCCTGT‑GCCTGGa                                                                                                                                            <  2:4778198/150‑1 (MQ=255)

        cAGCCTGCTGCATGAAAGTGGGCAGCCGCGTATTTTACTCGATGCCGTGACCTGCATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGC‑CATGTGCCTGGa                                                                                                                                            <  2:1634546/150‑1 (MQ=255)

        cAGCCTGCTGCATGAAAGTGGGCAGCCGCGTATTTTACTCGATGCCGTGACCTGCATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAACGGTCGATATTCTCCAGGCACATG‑GCCTGGa                                                                                                                                            <  1:2864937/150‑1 (MQ=255)

          gCCTGCTGCATGAAAGTGGGCAGCCGCGTATTTTACTCGATGCCGTGACCTGCATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGC‑CATGTGCCTGGAga                                                                                                                                          <  1:5537153/150‑2 (MQ=255)

           ccTGCTGCATGAAAGTGGGCAGCCGCGTATTTTACTCGATGCCGTGACCTGCATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGgt                                                                                                                                         <  1:9003521/150‑1 (MQ=255)

           ccTGCTGCATGAAAGTGGGCAGCCGCGTATTTTACTCGATGCCGTGACCTGCATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGgt                                                                                                                                         <  2:5797408/150‑1 (MQ=255)

           ccTGCTGCATGAAAGTGGGCAGCCGCGTATTTTACTCGATGCCGTGACCTGCATTCTCGACGACCAGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCCCCAGGCACATG‑GCCTGGAGgt                                                                                                                                         <  2:2873906/150‑1 (MQ=255)

             agctgcAAGAAAGTGGGCAGCCGCGTATTTTACTCGATGCCGTGACCTGCATTCTCGACGACCTGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGCACGATATTCTCCAGCCACATG‑GCCTGGAGgtgt                                                                                                                                       <  2:8650321/149‑1 (MQ=255)

              gctgcATGAAAGTGGGCAGCCGCGTATTTTACTCGATGCCGTGACCTGCATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGtt                                                                                                                                      <  1:5600801/150‑1 (MQ=255)

               ctgcATGAAAGTGGGCAGCCGCGTATTTTACTCGATGCCGTGACCTGCATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTa                                                                                                                                     <  1:6458184/150‑1 (MQ=255)

               ctgcATGAAAGTGGGCAGCCGCGTATTTTACTCGATGCCGTGACCTGCATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGC‑CATGTGCCTGGAgaatatc                                                                                                                                     <  1:5414037/150‑7 (MQ=255)

                  cATGAAAGTGGGCAGCCGCGTATTTTACTCGATGCCGTGACCTGCATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAAc                                                                                                                                  >  1:8229017/1‑150 (MQ=255)

                       aaGTGGGCAGCCGCGTATTTTACTCGATGCCGTGACCTGCATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGa                                                                                                                             <  1:6668729/150‑1 (MQ=255)

                       aaGTGGGCAGCCGCGTATTTTACTCGATGCCGTGACCTGCATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGa                                                                                                                             <  1:38477/150‑1 (MQ=255)

                          tGGGCAGCCGCGTATTTTACTCGATGCCGTGACCTGCATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACgg                                                                                                                          <  2:4841237/150‑1 (MQ=255)

                           gggCAGCCGCGTATTTTACTCGATGCCGTGACCTGCATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAAGGGTCGTTATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGc                                                                                                                         >  2:8765049/1‑150 (MQ=255)

                                  cgcgTATTTTACTCGATGCCGTGACCTGCATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTCATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCt                                                                                                                  >  2:2965929/1‑150 (MQ=255)

                                   gcgTATTTTACTCGATGCCGTGACCTGCATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCtt                                                                                                                 <  1:8831538/150‑1 (MQ=255)

                                   gcgTATTTTACTCGATGCCGTGACCTGCATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCtt                                                                                                                 <  1:502886/150‑1 (MQ=255)

                                    cgTATTTTACTCGATGCCGTGACCTGCATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCttt                                                                                                                <  1:7283758/150‑1 (MQ=255)

                                    cgTATTTTACTCGATGCCGTGACCTGCATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCttt                                                                                                                <  1:1543398/150‑1 (MQ=255)

                                    cgTATTTTACTCGATGCCGTGACCTGCATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCttt                                                                                                                <  2:8043017/150‑1 (MQ=255)

                                      tATTTTACTCGATGCCGTGACCTGCATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGt                                                                                                              <  1:8760802/150‑1 (MQ=255)

                                        ttttACTCGATGCCGTGACCTGCATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGttt                                                                                                            <  1:1095513/150‑1 (MQ=255)

                                          ttACTCGATGCCGTGACCTGCATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTAc                                                                                                          >  1:8626914/1‑150 (MQ=255)

                                            tcTCGATGCCGTGACCTGCATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGt                                                                                                        <  2:6697934/149‑1 (MQ=255)

                                            aCTCGATGCCGTGACCTGCATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGt                                                                                                        <  1:346464/150‑1 (MQ=255)

                                             cTCGATGCCGTGACCTGCATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTg                                                                                                       <  2:8484393/150‑1 (MQ=255)

                                             cTCGATGCCGTGACCTGCATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTg                                                                                                       >  1:6305865/1‑150 (MQ=255)

                                                gATGCCGTGACCTGCATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGa                                                                                                    >  1:9050903/1‑150 (MQ=255)

                                                 aTGCCGTGACCTGCATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGAt                                                                                                   >  1:64753/1‑150 (MQ=255)

                                                  tGCCGTGACCTGCATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATg                                                                                                  >  1:3013262/1‑150 (MQ=255)

                                                     cGTGACCTGCATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTcc                                                                                               >  2:3834727/1‑150 (MQ=255)

                                                        gACCTGCATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGc                                                                                            <  2:7657222/150‑1 (MQ=255)

                                                          ccTGCATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCgg                                                                                          <  2:3862702/150‑1 (MQ=255)

                                                          ccTGCATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCgg                                                                                          >  1:3213196/1‑150 (MQ=255)

                                                           cTGCATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTACTGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGc                                                                                         >  2:5546278/1‑150 (MQ=255)

                                                           cTGCATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTACTGGTCGAGATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCGTTGTTTACGTGCGATGTCCTGCGGc                                                                                         >  2:8582406/1‑150 (MQ=255)

                                                           cTGCATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGc                                                                                         >  1:7312817/1‑150 (MQ=255)

                                                            tGCATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCt                                                                                        >  1:7785602/1‑150 (MQ=255)

                                                              cATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCtgt                                                                                      <  2:2461483/150‑1 (MQ=255)

                                                              cATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCtgt                                                                                      <  2:2092767/150‑1 (MQ=255)

                                                              cATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCtgt                                                                                      <  2:2309439/150‑1 (MQ=255)

                                                              cATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCtgt                                                                                      <  1:4699059/150‑1 (MQ=255)

                                                                   tcGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAATCGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCa                                                                                 >  1:2421179/1‑150 (MQ=255)

                                                                   tcGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCa                                                                                 >  2:2551132/1‑150 (MQ=255)

                                                                     gacgacCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAAc                                                                               >  2:6384985/1‑150 (MQ=255)

                                                                     gacgacCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAAc                                                                               >  2:1131952/1‑150 (MQ=255)

                                                                     gacgacCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAAc                                                                               >  1:1900023/1‑150 (MQ=255)

                                                                       cgacCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACgg                                                                             <  1:7927406/150‑1 (MQ=255)

                                                                         acCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCa                                                                           <  2:1768747/150‑1 (MQ=255)

                                                                         acCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCa                                                                           <  1:1266851/150‑1 (MQ=255)

                                                                           cGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAgg                                                                         >  1:1968868/1‑150 (MQ=255)

                                                                                 ttCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTc                                                                   <  2:5013042/150‑1 (MQ=255)

                                                                                 ttCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTc                                                                   >  2:3295796/1‑150 (MQ=255)

                                                                                   cGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGc                                                                 <  1:3339912/150‑1 (MQ=255)

                                                                                    gCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACAGTCGcc                                                                >  2:1304291/1‑150 (MQ=255)

                                                                                     ccGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCa                                                               >  2:5850525/1‑150 (MQ=255)

                                                                                          cGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGTGGCTGTGAGCAACGGCAGGACATTCGCCACTGcc                                                          <  1:7086721/150‑1 (MQ=255)

                                                                                                tGCCAGTGCATAGCTCCGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCAGGGAc                                                    <  1:6777364/150‑1 (MQ=255)

                                                                                                   cAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCAGGGACttt                                                 >  1:6536536/1‑150 (MQ=255)

                                                                                                      tGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCAGGGACTTTTCg                                              <  1:2144602/150‑1 (MQ=255)

                                                                                                       gCATAGCTCAGCGACGTGCGGTACTGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCCCTGCCAGGGACTGTTCGc                                             >  2:1546752/1‑150 (MQ=255)

                                                                                                          tAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCAGGGACTTTTCGCAAt                                          <  2:4402937/150‑1 (MQ=255)

                                                                                                            gCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCAGGGACTTTTCGCAATAc                                        >  2:6913360/1‑150 (MQ=255)

                                                                                                             cTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCAGGGACTTTTCGCAATAcc                                       >  2:8243618/1‑150 (MQ=255)

                                                                                                              tCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCAGGGACTTTTCGCAATACCt                                      >  1:2649141/1‑150 (MQ=255)

                                                                                                              tCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCAGGGACTTTTCGCAATACCt                                      >  1:1752761/1‑150 (MQ=255)

                                                                                                              tCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCAGGGACTTTTCGCAATACCt                                      >  2:8910706/1‑150 (MQ=255)

                                                                                                              tCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCAGGGACTTTTCGCAATACCt                                      <  1:6913360/150‑1 (MQ=255)

                                                                                                                aGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCAGGGACTTTTCGCAATACCTgg                                    <  1:4525120/150‑1 (MQ=255)

                                                                                                                aGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCAGGGACTTTTCGCAATACCTgg                                    >  2:7139907/1‑150 (MQ=255)

                                                                                                                  cGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCAGGGACTTTTCGCAATACCTGGcc                                  >  2:5247236/1‑150 (MQ=255)

                                                                                                                            tAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCAGGGACTTTTCGCAATACCTGGCCACTGAGCTgg                        <  1:8261592/150‑1 (MQ=255)

                                                                                                                            tAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCAGGGACTTTTCGCAATACCTGGCCACTGAGCTgg                        <  1:5247236/150‑1 (MQ=255)

                                                                                                                            tAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCAGGGACTTTTCGCAATACCTGGCCACTGAGCTgg                        <  1:5893424/150‑1 (MQ=255)

                                                                                                                                 gTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCAGGGACTTTTCGCAATACCTGGCCACTGAGCTGGGTATc                   >  1:8459989/1‑150 (MQ=255)

                                                                                                                                 gTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCAGGGACTTTTCGCAATACCTGGCCACTGAGCTGGGTATc                   <  1:2921115/150‑1 (MQ=255)

                                                                                                                                 gTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCAGGGACTTTTCGCAATACCTGGCCACTGAGCTGGGTATc                   >  2:2008177/1‑150 (MQ=255)

                                                                                                                                  tCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCAGGGACTTTTCGCAATACCTGGCCACTGAGCTGGGTATcc                  >  2:4463280/1‑150 (MQ=255)

                                                                                                                                  tCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCAGGGACTTTTCGCAATACCTGGCCACTGAGCTGGGTATcc                  >  2:3530118/1‑150 (MQ=255)

                                                                                                                                    gATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCAGGGACTTTTCGCAATACCTGGCCACTGAGCTGAGTATCCTg                >  1:1373624/1‑150 (MQ=255)

                                                                                                                                      tatTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCAGGGACTTTTCGCAATACCTGGCCACTGAGCTGGGTATCCTGAc              <  1:4060445/150‑1 (MQ=255)

                                                                                                                                      tatTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCAGGGACTTTTCGCAATACCTGGCCACTGAGCTGGGTATCCTGAc              <  2:7863655/150‑1 (MQ=255)

                                                                                                                                        acctcCAGGC‑CATGTGCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCAGGGACTTTTCGCAATACCTGGCCACTGAGCTGGGTATCCTGACGa            >  2:4644853/3‑150 (MQ=255)

                                                                                                                                         cctcCAGGC‑CATGTGCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCAGGGACTTTTCGCAATACCTGGCCACTGAGCTGGGTATCCTGACGAt           >  1:3709608/2‑150 (MQ=255)

                                                                                                                                           tcCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCAGGGACTTTTCGCAATACCTGGCCACTGAGCTGGGTATCCTGACGATTc         >  2:5963/1‑150 (MQ=255)

                                                                                                                                           tcCAGGC‑CATGTGCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCAGGGACTTTTCGCAATACCTGGCCACTGAGCTGGGTATCCTGACGATTc         >  2:6536152/1‑150 (MQ=255)

                                                                                                                                            ccAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCAGGGACTTTTCGCAATACCTGGCCACTGAGCTGGGTATCCTGACGATTcc        <  2:4263924/150‑1 (MQ=255)

                                                                                                                                             cAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCAGGGACTTTTCGCAATACCTGGCCACTGAGCTGGGTATCCTGACGATTccc       >  2:3074439/1‑150 (MQ=255)

                                                                                                                                               ggCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCAGGGACTTTTCGCAATACCTGGCCACTGAGCTGGGTATCCTGACGATTCCCTa     <  2:2649141/150‑1 (MQ=255)

                                                                                                                                                gCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCAGGGACTTTTCGCAATACCTGGCCACTGAGCTGGGTATCCTGACGATTCCCTAt    <  2:2240165/150‑1 (MQ=255)

                                                                                                                                                 cacaTG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCAGGGACTTTTCGCAATACCTGGCCACTGAGCTGGGTATCCTGACGATTCCCTATc   <  1:6050134/150‑1 (MQ=255)

                                                                                                                                                  acaTG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCAGGGACTTTTCGCAATACCTGGCCACTGAGCTGGGTATCCTGACGATTCCCTATCa  <  1:1017232/150‑1 (MQ=255)

                                                                                                                                                  acaTG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCAGGGACTTTTCGCAATACCTGGCCACTGAGCTGGGTATCCTGACGATTCCCTATCa  >  2:6669257/1‑150 (MQ=255)

                                                                                                                                                  acaTG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCAGGGACTTTTCGCAATACCTGGCCACTGAGCTGGGTATCCTGACGATTCCCTATCa  <  2:6034349/150‑1 (MQ=255)

                                                                                                                                                  acaTG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCAGGGACTTTTCGCAATACCTGGCCACTGAGCTGGGTATCCTGACGATTCCCTATCa  <  2:3973943/150‑1 (MQ=255)

GGCAAGCTCAGCCTGCTGCATGAAAGTGGGCAGCCGCGTATTTTACTCGATGCCGTGACCTGCATTCTCGACGACCGGGGCTTCGCCGAACGCCTTTGCCAGTGCATAGCTCAGCGACGTGCGGTAATGGTCGATATTCTCCAGGCACATG‑GCCTGGAGGTGTTAAACGCTGACGGCACATTCTTTGTTTACGTGCGATGTCCTGCGGCTGTGAGCAACGGCAGGACATTCGCCACTGCCAGGGACTTTTCGCAATACCTGGCCACTGAGCTGGGTATCCTGACGATTCCCTATCA  >  NC_002947/4316551‑4316846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`