BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,842,848	T→G	14.5%	N663T (AAC→ACC)	gacS ←	sensor protein GacS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,842,848	0	T	G	14.5%	489.9 / 9.8	206	N663T (AAC→ACC)	gacS	sensor protein GacS
Reads supporting (aligned to +/- strand): ref base T (66/103); new base G (34/2); total (101/105)
Fisher's exact test for biased strand distribution p-value = 2.44e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 212 total aligned reads displayed.

GAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCGGTGGTCGGG  >  NC_002947/1842700‑1842997

gAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGtt                                                                                                                                                      >  1:1683987/1‑150 (MQ=255)

gAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGtt                                                                                                                                                      >  1:8980364/1‑150 (MQ=255)

 aaCAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTg                                                                                                                                                     >  1:5123211/1‑150 (MQ=255)

 aaCAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTg                                                                                                                                                     <  2:3051554/150‑1 (MQ=255)

 aaCAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTg                                                                                                                                                     >  2:5631379/1‑150 (MQ=255)

   cAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTggg                                                                                                                                                   <  2:9014910/150‑1 (MQ=255)

   cAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTggg                                                                                                                                                   <  2:8438106/150‑1 (MQ=255)

   cAATACTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGGTggg                                                                                                                                                   >  1:5478140/1‑150 (MQ=255)

    aaTTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTgggg                                                                                                                                                  >  2:8560546/1‑150 (MQ=255)

    aaTTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTgggg                                                                                                                                                  >  2:5952695/1‑150 (MQ=255)

     aTTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGt                                                                                                                                                 <  1:6343305/150‑1 (MQ=255)

     aTTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGt                                                                                                                                                 <  1:1744297/150‑1 (MQ=255)

         ttcCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCt                                                                                                                                             >  2:2796384/1‑150 (MQ=255)

            cTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTccc                                                                                                                                          <  1:3218036/150‑1 (MQ=255)

            cTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTccc                                                                                                                                          <  1:7835431/150‑1 (MQ=255)

            cTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGGTGGGGGTGGTccc                                                                                                                                          >  1:3472244/1‑150 (MQ=255)

             tGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑GCGTTGGGGTTTGTcccc                                                                                                                                         >  1:3353042/1‑150 (MQ=255)

             tGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTcccc                                                                                                                                         >  2:6749456/1‑150 (MQ=255)

                aCGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCTCg                                                                                                                                      <  1:2072844/150‑1 (MQ=255)

                aCGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGGTGGGGTTGCTCCCCTCg                                                                                                                                      >  1:7573610/1‑150 (MQ=255)

                  ggCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCTCGGc                                                                                                                                    <  1:4801940/150‑1 (MQ=255)

                  ggCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCTCGGc                                                                                                                                    <  1:4801960/150‑1 (MQ=255)

                     cTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGGTTTGGTTGCGCCCCGCGGCCAg                                                                                                                                 >  1:2709764/1‑150 (MQ=255)

                        tACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGGTGGGGGTGCTACCCACGGcccccgg                                                                                                                              >  1:4158040/1‑145 (MQ=255)

                         aCCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCTCGGCCAGCGGa                                                                                                                             <  2:5221164/150‑1 (MQ=255)

                            gccgcATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCTCGGCCAGCGGACgg                                                                                                                          <  2:260142/150‑1 (MQ=255)

                                 aTAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCTGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAgg                                                                                                                     <  1:5028330/150‑1 (MQ=255)

                                  tAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGt                                                                                                                    >  2:5679559/1‑150 (MQ=255)

                                    aCCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTg                                                                                                                  >  2:7549254/1‑150 (MQ=255)

                                      cATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCa                                                                                                                >  1:7436238/1‑150 (MQ=255)

                                      cATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGGTGGGGTTGCTCCCCACGGCCCCCGGACGGCGAGGGTGCa                                                                                                                >  2:1762614/1‑150 (MQ=255)

                                          gttgACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTGGCTTG‑GCGTTGGGGTTTGTACCCTCGGCCAGCGGACGGCGAGGTTGGACCAg                                                                                                            >  1:1104244/1‑150 (MQ=255)

                                              aCGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCACGGCCCGCGGACGGCGAGGTTGCACCAGCAcc                                                                                                        >  2:1171861/1‑150 (MQ=255)

                                                  ccAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTACCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCa                                                                                                    >  2:4427711/1‑150 (MQ=255)

                                                   cAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCaa                                                                                                   >  2:2122097/1‑150 (MQ=255)

                                                   cAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCGCCGCCAGCGGACGGCGAGGTTTCACCAGCGCCACCaa                                                                                                   >  1:8853087/1‑150 (MQ=255)

                                                   cAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGGTGGGGTTGCTCCCCACGGCCCGCGGACGGCGAGGGTGCACCAGCGCCCGcca                                                                                                   >  2:531873/1‑148 (MQ=255)

                                                   cAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCGGG‑CCGTTGGGGTTGCTCCCCTCGGCCCGCGGACGGCGAGGGTGCACCAGCGCCAGCac                                                                                                   >  2:5182878/1‑149 (MQ=255)

                                                    aGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑GCGTTGGGGTTTGTACCCACGGCCACCGGACGGCGAGGTTTGACCAGCACCAGCaat                                                                                                  >  1:1878198/1‑149 (MQ=255)

                                                     gTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAaga                                                                                                 <  1:4275293/150‑1 (MQ=255)

                                                      tACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAagag                                                                                                <  1:8310573/150‑1 (MQ=255)

                                                         cTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGgcg                                                                                             >  2:4480758/1‑150 (MQ=255)

                                                         cTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCAGTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCCAGAGgcg                                                                                             >  2:9082897/1‑150 (MQ=255)

                                                          tCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCCGTTGGGTTTG‑TCCCCACCGCCACCGGACGCCGAGGTTTCACCAGCAACAGCAACAGgcgc                                                                                            >  1:7165920/1‑150 (MQ=255)

                                                          tCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGgcgc                                                                                            <  1:636490/150‑1 (MQ=255)

                                                          tCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCGGG‑GCGGTGGGGTTTGTGCCCACGGGCCGCGGACGGGGGGGTTGGAACAGGAACAGCAAGAGgcgc                                                                                            >  1:4095079/1‑150 (MQ=255)

                                                             gCACCCAGGTCTTCGAGCTGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGcgccgc                                                                                         <  1:4222562/150‑1 (MQ=255)

                                                                 ccAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCt                                                                                                          >  2:7712106/1‑129 (MQ=255)

                                                                 ccAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCt                                                                                                          <  1:7712106/129‑1 (MQ=255)

                                                                 ccAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCGCGGCCAGCGGGCGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCt                                                                                     >  1:3386835/1‑150 (MQ=255)

                                                                 ccAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCACGGCCAGCGGACGGCGCGGTTGCACCAGCTCCAGCACGAGGCGCCGCAGCt                                                                                     >  2:515666/1‑150 (MQ=255)

                                                                  cAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCTCGGCCCGCGGACGGCGCGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCtt                                                                                    >  2:5490919/1‑150 (MQ=255)

                                                                  cAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCtt                                                                                    >  2:8923285/1‑150 (MQ=255)

                                                                   aGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTg                                                                                   <  2:8229968/150‑1 (MQ=255)

                                                                   aGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTg                                                                                   <  2:4940017/150‑1 (MQ=255)

                                                                   aGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCGCCGCCAGCGGACGGCCAGGTTGCACCCCCACCACCAAGAGGCGCCGCAGCATg                                                                                   >  1:5905572/1‑150 (MQ=255)

                                                                   aGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCAGG‑CCCGTGGGGTTGCTACCCACCGCCACCCGACCGCGAGGGTGGAACACCAACACCAAGAGGCACCCCaccatt                                                                                   >  1:2534978/1‑145 (MQ=255)

                                                                     gTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCg                                                                                 <  2:1934805/150‑1 (MQ=255)

                                                                      tCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCgg                                                                                <  2:4900164/150‑1 (MQ=255)

                                                                      tCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCgg                                                                                <  2:648178/150‑1 (MQ=255)

                                                                        ttCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCGGG‑GCGGTGGGGGTGCTCCCCCCCGCCCCCCGCCCGCCGGGTTTAACCACCGCCACCAACAGGCGCCCCCCCGTGCgggg                                                                              >  1:188611/1‑149 (MQ=255)

                                                                        ttCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCGGG‑CCGGTGGGGTTTCTCCCCCCCGCCCGCCGACCGCGCGGGTTCACCACCAACAACCACAGGCGCCCCACCATGCGGGt                                                                              >  1:7929426/1‑150 (MQ=255)

                                                                        ttCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCGGG‑CCCTTGGGGTTTCAACCCCCGGCCCGCCGGCGGCGAGGTTTCACCCCCTCCACCAACAGGCGCCGCCGCGTGCGGGt                                                                              >  1:2800625/1‑150 (MQ=255)

                                                                           gAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGAGct                                                                           <  2:793837/150‑2 (MQ=255)

                                                                            aGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAg                                                                          <  2:7370082/150‑1 (MQ=255)

                                                                               aGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTc                                                                       >  2:5102508/1‑150 (MQ=255)

                                                                                 ggTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCgg                                                                     >  2:6663442/1‑150 (MQ=255)

                                                                                  gTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGc                                                                    <  2:7290857/150‑1 (MQ=255)

                                                                                     tttACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTg                                                                 <  2:7415199/150‑1 (MQ=255)

                                                                                         ccAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACa                                                             <  2:3847674/150‑1 (MQ=255)

                                                                                          cagcagcagGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCCCTGG‑CCCGTGGGGTTGCTCACCTCGGCCCGCCGACCGCGAGGTTGCACCACCTCCAGCCAGAGGCGCCCCCGCGTGCGGGTGCAGGTCGGCTTGGACac                                                            >  2:6997648/1‑149 (MQ=255)

                                                                                            gcagcagGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑GCCTTGGGGTTGCTCCCCTCGGCCAGCCGCAGGCGAGGTTTCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGGCGGCTTGGACAGCa                                                          >  1:2857813/1‑150 (MQ=255)

                                                                                                cagGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTg                                                      <  1:8753127/150‑1 (MQ=255)

                                                                                                 agGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTgg                                                     <  1:8229529/150‑1 (MQ=255)

                                                                                                 agGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCACGGCCAGCGGACCGCGAGGTTGCACCCGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTgg                                                     >  2:1983870/1‑150 (MQ=255)

                                                                                                  ggTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGc                                                    <  1:3738756/150‑1 (MQ=255)

                                                                                                       gcggcATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTTG‑GCGTTGGGGTTGCTCCCCTCGGCCAGCGGAAGGCGAGGTTGCACCAGCAACAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACAGGCCAAgg                                               >  2:6335805/1‑150 (MQ=255)

                                                                                                       gcggcATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATgg                                               <  1:5700693/150‑1 (MQ=255)

                                                                                                        cggcATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGc                                              >  2:1476076/1‑150 (MQ=255)

                                                                                                            aTTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGtt                                          <  2:4105690/150‑1 (MQ=255)

                                                                                                             ttGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTg                                         <  2:6406195/150‑1 (MQ=255)

                                                                                                             ttGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTg                                         <  1:4357510/150‑1 (MQ=255)

                                                                                                                tcgtcgACGCAGAGTAGATTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGc                                      <  1:8948591/150‑1 (MQ=255)

                                                                                                                  gtcgACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAg                                    <  2:3097402/150‑1 (MQ=255)

                                                                                                                  gtcgACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAg                                    <  2:6571398/150‑1 (MQ=255)

                                                                                                                        gCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACgg                              <  1:2434995/150‑1 (MQ=255)

                                                                                                                         cAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACggg                             <  1:3133955/150‑1 (MQ=255)

                                                                                                                          agagTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCGCGGCCAGCGGCCGGCGCGGTTGCACCAGCTCCAGCCAGAGGCGCCCCAGCGTGCGGGTGCAGGTCGGCTTGGACAGCAACAGGCCAAGGCCGTTGGGCAGGTACcggg                            >  2:7317811/1‑149 (MQ=255)

                                                                                                                           gagTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGGGAGGTTGGACCAGCTCCAGGAAGAGGCGCCGCAGCTTGCGGGTGCAGGTGGGCTTTGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGggtg                           >  2:2992186/1‑150 (MQ=255)

                                                                                                                             gTATTTTCGGTCGTCGCTGG‑GCGTTTGGGTTGGTACCCTCGGCCAGCGGACGGGGAGGTTTCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGGTTGGTCAGCAACATGGCAAGGCCGTTGGGCAGGTACGggtggt                         >  2:3270586/1‑150 (MQ=255)

                                                                                                                                ttttCGGTCGTCGCTGG‑CCCTTGGGGTTTCTACCCACGGCCCCCGGACCGCGAGGTTTCACCACCGCCAGCCAGAGGCGCCCCAGCGTGCGGGTGCAGGGCGGCTTGGGCACCAACAGGCCAAGGCCCTTGGGCCGGTACGGGTGGTACa                      >  2:1005139/1‑150 (MQ=255)

                                                                                                                                         gtcgCTGG‑CCGTTGGGGTTGCTACCCACGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCt             >  2:2664384/1‑150 (MQ=255)

                                                                                                                                            gCTGG‑CCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCgg          <  1:443509/150‑1 (MQ=255)

                                                                                                                                            gCTGG‑CCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCgg          <  2:8980368/150‑1 (MQ=255)

                                                                                                                                                  ccGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCCCgaccacc     <  2:1882931/150‑7 (MQ=255)

                                                                                                                                                   cGTTGGGGTTGCTCCCCTCGGCCAGTGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGATGGGCAGGTACGGGTGGTACAGCGCCTGCTCGGTGGTCg    >  2:1398438/1‑150 (MQ=255)

                                                                                                                                                    gTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCGGTGGTCgg   >  1:3660902/1‑150 (MQ=255)

                                                                                                                                                    gTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCGGTGGTCgg   >  2:4860775/1‑150 (MQ=255)

                                                                                                                                                    gTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCGGTGGTCgg   <  1:3056041/150‑1 (MQ=255)

                                                                                                                                                     ttGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCGGTGGTCggg  >  2:2371558/1‑150 (MQ=255)

GAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGG‑CCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCGGTGGTCGGG  >  NC_002947/1842700‑1842997

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`