Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,253,240 C→A 6.7% N202K (AAC→AAA) ‡ leuC → 3‑isopropylmalate dehydratase large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,253,2400CA 6.7% 1343.1 / 60.2 400N202K (AAC→AAA) ‡leuC3‑isopropylmalate dehydratase large subunit
Reads supporting (aligned to +/- strand):  ref base C (221/152);  new base A (14/13);  total (235/165)
Fisher's exact test for biased strand distribution p-value = 5.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 403 total aligned reads displayed.

GAGGTCGAGCACGTGCTCGCCACCCAGTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGAAGGGCGCCC  >  NC_002947/2253091‑2253389
                                                                                                                                                     |                                                                                                                                                     
   gTCGAGCACGTGCTCGCCACCCAGTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGt                                                                                                                                                    >  2:7803585/1‑150 (MQ=255)
   gTCGAGCACGTGCTCGCCACCCAGTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGt                                                                                                                                                    <  2:5231832/150‑1 (MQ=255)
      gAGCACGTGCTCGCCACCCAGTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACg                                                                                                                                                      <  2:4134757/145‑1 (MQ=255)
      gAGCACGTGCTCGCCACCCAGTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCAc                                                                                                                                                 >  2:1238625/1‑150 (MQ=255)
      gAGCACGTGCTCGCCACCCAGTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCAc                                                                                                                                                 >  2:8465703/1‑150 (MQ=255)
      gAGCACGTGCTCGCCACCCAGTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCAc                                                                                                                                                 >  2:4369509/1‑150 (MQ=255)
       ttccactGCTCGCCACCCAGTGCCTGGTCGCCAAGAAGATGCAGAACCTGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCCAGGATAGCGTGCTGGCCGTGCTCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACg                                                                                                                                                >  2:8245923/7‑150 (MQ=255)
           cGTGCTCGCCACCCAGTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCag                                                                                                                                            >  1:9008342/1‑149 (MQ=255)
                  gCCACCCAGTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTc                                                                                                                                     <  1:8300529/150‑1 (MQ=255)
                   ccACCCAGTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGAGCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCg                                                                                                                                    >  1:544742/1‑150 (MQ=255)
                    cACCCAGTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCgc                                                                                                                                   <  2:5495343/150‑1 (MQ=255)
                    cACCCAGTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCgc                                                                                                                                   >  1:2218650/1‑150 (MQ=255)
                     aCCCAGTGCCTGGTCGCCAAGAAGAGGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCgca                                                                                                                                  <  1:8667633/150‑1 (MQ=255)
                      cccAGTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCgcag                                                                                                                                 >  1:3697837/1‑150 (MQ=255)
                      cccAGTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCgcag                                                                                                                                 <  1:5173235/150‑1 (MQ=255)
                      cccAGTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCgcag                                                                                                                                 >  2:1064251/1‑150 (MQ=255)
                        cAGTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCACGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCgcaggc                                                                                                                               >  2:7402058/1‑150 (MQ=255)
                         aGTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCgcaggca                                                                                                                              <  1:501302/150‑1 (MQ=255)
                               tGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCa                                                                                                                        >  1:3917983/1‑150 (MQ=255)
                                 gTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCAtt                                                                                                                      >  1:6462536/1‑150 (MQ=255)
                                   cGCCAAGAAGATGAAGAACAAGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGCTCACGCCAAGGAATTCGCAGGCAGAGCCATTcg                                                                                                                    >  2:5838340/1‑150 (MQ=255)
                                    gCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTcgc                                                                                                                   <  1:7608529/150‑1 (MQ=255)
                                       aagaagATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGACTTCGCAGGCAGTGCCATTCGCGaa                                                                                                                >  2:2058023/1‑150 (MQ=255)
                                       aagaagATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGaa                                                                                                                >  2:1941001/1‑150 (MQ=255)
                                       aagaagATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGaa                                                                                                                >  2:708813/1‑150 (MQ=255)
                                         gaagaTGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAAtt                                                                                                              <  1:6598383/150‑1 (MQ=255)
                                         gaagaTGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAAtt                                                                                                              <  2:124267/150‑1 (MQ=255)
                                         gaagaTGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAAtt                                                                                                              <  2:3540846/150‑1 (MQ=255)
                                             aTGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCg                                                                                                          >  1:2564710/1‑150 (MQ=255)
                                               gaagaaCATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGAt                                                                                                        >  1:9050442/1‑150 (MQ=255)
                                                aagaaCATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATg                                                                                                       <  1:5890249/150‑1 (MQ=255)
                                                  gaaCATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGa                                                                                                     >  1:4505839/1‑150 (MQ=255)
                                                  gaaCATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGa                                                                                                     >  1:7312240/1‑150 (MQ=255)
                                                   taCATGCTGGTGCGAGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGaa                                                                                                    <  2:8763971/149‑1 (MQ=255)
                                                   aaCATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGaa                                                                                                    <  1:802769/150‑1 (MQ=255)
                                                        gCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCg                                                                                               >  1:1010383/1‑150 (MQ=255)
                                                         cTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGc                                                                                              <  1:3454218/150‑1 (MQ=255)
                                                             tGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGACTTGTCGATGGAAGGCCGCATGa                                                                                          >  2:8995837/1‑150 (MQ=255)
                                                                gcgTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCa                                                                                       >  1:8413204/1‑150 (MQ=255)
                                                                  gTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATc                                                                                     >  1:6153460/1‑150 (MQ=255)
                                                                      aaGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCCTTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCa                                                                                 >  1:3030443/1‑150 (MQ=255)
                                                                      aaGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCa                                                                                 <  2:4331427/150‑1 (MQ=255)
                                                                       aGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCaa                                                                                <  2:8210220/150‑1 (MQ=255)
                                                                       aGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCaa                                                                                <  1:8948902/150‑1 (MQ=255)
                                                                       aGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCaa                                                                                <  2:6166571/150‑1 (MQ=255)
                                                                        ggCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAAc                                                                               >  2:4805175/1‑150 (MQ=255)
                                                                           cAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATg                                                                            >  2:1857282/1‑150 (MQ=255)
                                                                           cAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATg                                                                            <  2:8319236/150‑1 (MQ=255)
                                                                           aattggccttccGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATg                                                                            >  1:7126557/6‑150 (MQ=255)
                                                                            aaTTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGt                                                                           >  1:2776828/1‑150 (MQ=255)
                                                                            aaTTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGt                                                                           <  2:2830340/150‑1 (MQ=255)
                                                                                ggctccaggCGTCACCGCCAAGGACATCACGCTTGCAATCATTGGCGAGATTGGCACGGCTGGCGGCACAGGCTATGTGATCGAATATGCTGGCAGCGCCATTCGCTCGCTGTCGATGGAAGGCCGCATGACGGTCTGCAACATGTCGAt                                                                       <  1:2858715/143‑1 (MQ=255)
                                                                                gcctgccGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCAt                                                                       >  2:2440975/1‑150 (MQ=255)
                                                                                 gctccaggCGTCACCGCCAAGGACATCACGCTTGCAATCATTGGCGAGATTGGCACGGCTGGCGGCACAGGCTATGTGATCGAATATGCTGGCAGCGCCATTCGCTCGCTGTCGATGGAAGGCCGCATGACGGTCTGCAACATGTCGAtt                                                                      <  1:2099398/144‑2 (MQ=255)
                                                                                 cctgccGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTc                                                                                                           <  2:2643853/113‑1 (MQ=255)
                                                                                 cctgccGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTc                                                                                                           >  1:2643853/1‑113 (MQ=255)
                                                                                   tgccGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCTGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGa                                                                    >  1:1350525/1‑150 (MQ=255)
                                                                                   tgccGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCAGTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGa                                                                    >  1:3168702/1‑150 (MQ=255)
                                                                                   tccaggCGTCACCGCCAAGGACATCACGCTTGCAATCATTGGCGAGATTGGCACGGCTGGCGGCACAGGCTATGTGATCGAATATGCTGGCAGCGCCATTCGCTCGCTGTCGATGGAAGGCCGCATGACGGTCTGCAACATGTCGATTGa                                                                    >  1:3783491/5‑150 (MQ=255)
                                                                                    gccGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGaa                                                                   >  1:4743734/1‑150 (MQ=255)
                                                                                     caggCGTCACCGCCAAGGACATCACGCTTGCAATCATTGGCGAGATTGGCACGGCTGGCGGCACAGGCTATGTGATCGAATATGCTGGCAGCGCCATTCGCTCGCTGTCGATGGAAGGCCGCATGACGGTCTGCAACATGTCGATTGAAg                                                                  >  1:7790802/3‑150 (MQ=255)
                                                                                      aggCGTCACCGCCAAGGACATCACGCTTGCAATCATTGGCGAGATTGGCACGGCTGGCGGCACAGGCTATGTGATCGAATATGCTGGCAGCGCCATTCGCTCGCTGTCGATGGAAGGCCGCATGACGGTCTGCAACATGTCGATTGAAgg                                                                 >  1:1615685/2‑149 (MQ=255)
                                                                                          gTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGgc                                                             >  1:6619881/1‑150 (MQ=255)
                                                                                          gTCACCGCCAAGGACATCACGCTTGCAATCATTGGCGAGATTGGCACGGCTGGCGGCACAGGCTATGTGATCGAATATGCTGGCAGCGCCATTCGATCGCTGTCGATGGAAGGCCGCATGACGGTCTGCAACATGTCGATTGAAggcggc                                                             <  1:5368776/150‑1 (MQ=255)
                                                                                             aCCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGcc                                                          >  2:453105/1‑150 (MQ=255)
                                                                                             aCCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGcc                                                          >  1:6946132/1‑150 (MQ=255)
                                                                                                gCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCcgc                                                       >  2:6386186/1‑150 (MQ=255)
                                                                                                gCCAAGGACATCACGCTTGCAATCATTGGCGAGATTGGCACGGCTGGCGGCACAGGCTATGTGATCGAATATGCTGGCAGCGCCATTCGCTCGCTGTCGATGGAAGGCCGCATGACGGTCTGCAACATGTCGATTGAAGGCGGCGCAcgc                                                       <  2:173686/150‑1 (MQ=255)
                                                                                                 ccAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCcgcg                                                      >  1:2350534/1‑150 (MQ=255)
                                                                                                 ccAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCcgcg                                                      >  2:5012636/1‑150 (MQ=255)
                                                                                                  cAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGt                                                     >  2:3027790/1‑150 (MQ=255)
                                                                                                  cAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGt                                                     >  2:2537372/1‑150 (MQ=255)
                                                                                                  cAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGt                                                     >  2:762333/1‑150 (MQ=255)
                                                                                                        tatCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCt                                               >  2:1497064/1‑150 (MQ=255)
                                                                                                          tCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCtgg                                             <  1:7486136/150‑1 (MQ=255)
                                                                                                          tCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCtgg                                             >  1:249397/1‑150 (MQ=255)
                                                                                                           tgTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCtggt                                            <  2:213899/149‑1 (MQ=255)
                                                                                                            gTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCtggtg                                           >  1:1135582/1‑150 (MQ=255)
                                                                                                             tGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCCTTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCtggtgg                                          >  1:7529696/1‑150 (MQ=255)
                                                                                                              gCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGc                                         >  2:575183/1‑150 (MQ=255)
                                                                                                                 ggCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAAc                                      >  1:1484064/1‑150 (MQ=255)
                                                                                                                 ggCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAAc                                      <  1:4117161/150‑1 (MQ=255)
                                                                                                                    cGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGa                                   >  2:407382/1‑150 (MQ=255)
                                                                                                                    cGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGa                                   <  2:8635516/150‑1 (MQ=255)
                                                                                                                     gTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGAt                                  <  1:3283149/150‑1 (MQ=255)
                                                                                                                      tGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATg                                 >  1:190506/1‑150 (MQ=255)
                                                                                                                          cGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCCTTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCACCCGATGCCAc                             >  2:8087276/1‑150 (MQ=255)
                                                                                                                            gCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCATCGGTTGCCAcca                           <  2:3364236/150‑3 (MQ=255)
                                                                                                                              aaGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTAcc                         <  1:6693106/150‑1 (MQ=255)
                                                                                                                               aGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCg                        <  2:402438/150‑1 (MQ=255)
                                                                                                                                   cGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGc                    <  2:8862289/150‑1 (MQ=255)
                                                                                                                                     gCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCCTTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCt                  >  2:653087/1‑150 (MQ=255)
                                                                                                                                         cgccgGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGt              <  2:7201390/150‑1 (MQ=255)
                                                                                                                                         cgccgGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGt              <  2:3777588/150‑1 (MQ=255)
                                                                                                                                             ggTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGaa          <  1:3062514/150‑1 (MQ=255)
                                                                                                                                                 gCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGAAGGgc      <  2:6722500/150‑1 (MQ=255)
                                                                                                                                                 gCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGAAGGgc      >  2:5607641/1‑150 (MQ=255)
                                                                                                                                                   aaCGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGAAGGgcgc    >  1:179932/1‑150 (MQ=255)
                                                                                                                                                    aCGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGAAGGGCGcc   <  1:5819949/150‑1 (MQ=255)
                                                                                                                                                     cGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGAAGGGCGccc  <  1:4369509/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GAGGTCGAGCACGTGCTCGCCACCCAGTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGAAGGGCGCCC  >  NC_002947/2253091‑2253389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑