BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,273,632	A→G	5.3%	intergenic (+51/‑49)	PP_t32 → / → PP_t33	tRNA‑Asp/tRNA‑Asp

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,273,632	0	A	G	5.3%	648.3 / 14.6	204	intergenic (+51/‑49)	PP_t32/PP_t33	tRNA‑Asp/tRNA‑Asp
Reads supporting (aligned to +/- strand): ref base A (104/88); new base G (3/8); total (107/97)
Fisher's exact test for biased strand distribution p-value = 1.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 209 total aligned reads displayed.

AGCCAGAGAGGAACGCACTGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGTTTCAAGGTCCCTAG  >  NC_002947/2273484‑2273778

   cAGAGAGGAACGCACTGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGcc                                                                                                                                                >  1:2580838/1‑150 (MQ=255)

    agagagGAACGCACTGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCt                                                                                                                                               >  2:3256357/1‑150 (MQ=255)

    agagagGAACGCACTGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCt                                                                                                                                               >  2:4064234/1‑150 (MQ=255)

    agagagGAACGCACTGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTGCAGTAACAGATGGGTGACTGGCACCGGTCTGAAGCCACAAGGCACATGCCt                                                                                                                                               >  1:3208689/1‑150 (MQ=255)

    agagagGAACGCACTGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATGTTTCAGTCACAGATGGGTGCCAGGCACCGGTCGGAAGCCACAAGGCACATGCCt                                                                                                                                               >  2:4187925/1‑150 (MQ=255)

     gagagGAACGCACTGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCtt                                                                                                                                              <  1:7884705/150‑1 (MQ=255)

      agagGAACGCACTGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTg                                                                                                                                             <  1:7226812/150‑1 (MQ=255)

       gagGAACGCACTGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTgg                                                                                                                                            <  1:1698220/150‑1 (MQ=255)

        agGAACGCACTGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTggg                                                                                                                                           <  2:7305039/150‑1 (MQ=255)

           aaCGCACTGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTg                                                                                                                                        >  1:739912/1‑150 (MQ=255)

           aaCGCACTGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCC‑CAAGGCATGTGCCTTgtggctt                                                                                                                                       <  2:3870788/150‑7 (MQ=21)

            aCGCACTGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCC‑CAAGGCATGTGCCTTgtggcttc                                                                                                                                      <  1:4107613/150‑8 (MQ=21)

              gCACTGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATc                                                                                                                                     <  2:7057781/150‑1 (MQ=255)

                aCTGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGACTGGAACCGGTCTGAAGCCACAAGGCACATGACTTGGGCTGATccc                                                                                                                                   >  1:4459173/1‑150 (MQ=255)

                aCTGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTACAGTCACAGATGGGTGCCTGGCACCGGACGGACGCCACTAGGCACATGCCTTGGGCTGATccc                                                                                                                                   >  2:51348/1‑150 (MQ=21)

                     gcAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGttt                                                                                                                              <  1:3464579/150‑1 (MQ=255)

                        gCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTTCGCCATTTTTCAGTAACAGATGGGTGACTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCaa                                                                                                                           >  2:1903487/1‑150 (MQ=255)

                        gCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTGTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTcta                                                                                                                           <  2:7619139/150‑3 (MQ=255)

                           gTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCg                                                                                                                        <  1:8033264/150‑1 (MQ=255)

                            tAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCgg                                                                                                                       <  1:5234031/150‑1 (MQ=255)

                            tAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCgg                                                                                                                       <  1:7165950/150‑1 (MQ=255)

                                  aGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCaa                                                                                                                 <  1:369538/150‑1 (MQ=255)

                                  aGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCaa                                                                                                                 <  2:1093221/150‑1 (MQ=255)

                                  aGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCaa                                                                                                                 <  2:5678110/150‑1 (MQ=255)

                                  aGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCAAATTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCACATTCAATCGGGCGCaa                                                                                                                 <  2:8328283/150‑1 (MQ=255)

                                    tCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGc                                                                                                               <  2:6092895/150‑1 (MQ=255)

                                      ggTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCt                                                                                                             <  2:3735668/150‑1 (MQ=255)

                                      ggTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCt                                                                                                             <  2:2258687/150‑1 (MQ=255)

                                       gTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTg                                                                                                            <  1:4481488/150‑1 (MQ=255)

                                          aGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCAGGCACCGGTCTGAAGCCACAAGGCACATCCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAAc                                                                                                         >  2:5572105/1‑150 (MQ=255)

                                            acccccGGCGTGACAGGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGa                                                                                                       >  2:5476080/5‑150 (MQ=255)

                                              ccccGGCGTGACAGGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCAAGAACGAtc                                                                                                     >  2:1618721/3‑149 (MQ=38)

                                                   gCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAg                                                                                                <  2:8456907/150‑1 (MQ=255)

                                                   gCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAg                                                                                                >  1:8876781/1‑150 (MQ=255)

                                                    ccTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGc                                                                                               >  1:1562423/1‑150 (MQ=255)

                                                    ccTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGc                                                                                               <  1:3103230/150‑1 (MQ=255)

                                                         caggCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAg                                                                                          <  1:5476080/147‑1 (MQ=255)

                                                          aCGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGt                                                                                         <  1:4239110/150‑1 (MQ=255)

                                                          aCGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCAGAACGATACGCAGCGGGAGt                                                                                         >  2:8120241/1‑150 (MQ=255)

                                                             ccGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCa                                                                                      <  1:470614/150‑1 (MQ=255)

                                                               gggggTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCTGCGGTAGTTCAGt                                                                                    <  1:1266418/150‑1 (MQ=255)

                                                                     cgcgGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTa                                                                              <  1:2116592/150‑1 (MQ=255)

                                                                     cgcgGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTa                                                                              <  1:197930/150‑1 (MQ=255)

                                                                     cgcgGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTa                                                                              <  2:497119/150‑1 (MQ=255)

                                                                     cgcgGGTTCGAGTCCCGTCCGCTGCGCCATTTGTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTa                                                                              <  2:1524116/150‑1 (MQ=255)

                                                                      gcgGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGcc                                                                                                                                                >  1:5842949/1‑83 (MQ=255)

                                                                            tCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATAcc                                                                       <  1:105984/150‑1 (MQ=255)

                                                                              gAGTCCCGTCCGCTGCGCCATTTTTCATTAAAAGATGGTTGACTGGCACCTGTCTGAAGCAACAAGTCACATGCCTTTGTCTGATACCATTTTCATTCGGTCTCAAGCATGAACGATAAGCAGCGGTAGTTCATTCGGTTAGAAAAACgg                                                                     >  1:2932259/1‑150 (MQ=255)

                                                                               aGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGc                                                                    <  1:3161547/150‑1 (MQ=255)

                                                                               aGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGc                                                                    <  1:4923079/150‑1 (MQ=255)

                                                                                gTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGcc                                                                   >  1:3668193/1‑150 (MQ=255)

                                                                                 tCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCt                                                                  <  2:2407420/150‑1 (MQ=255)

                                                                                    cGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTc                                                               >  1:7670356/1‑150 (MQ=255)

                                                                                    cGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTc                                                               >  2:1464525/1‑150 (MQ=255)

                                                                                    cGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTc                                                               >  2:3557101/1‑150 (MQ=255)

                                                                                           cgcgcCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTTAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCTCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCgg                                                        <  1:3995668/149‑1 (MQ=255)

                                                                                           cgcgcCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCgg                                                        <  2:203909/149‑1 (MQ=255)

                                                                                             cgcCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCgggg                                                      <  2:2843523/150‑1 (MQ=255)

                                                                                             cgcCATCTTTCAGTAACAGATGGGTGCCTGGCACCGGTCAGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCgggg                                                      <  1:8584091/150‑1 (MQ=255)

                                                                                              gcCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCggggg                                                     <  1:7470231/150‑1 (MQ=255)

                                                                                               ccATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCtt                                                                                                                                              <  1:3248648/60‑1 (MQ=255)

                                                                                               ccATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGt                                                    >  1:3650478/1‑150 (MQ=255)

                                                                                                 aTTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTcg                                                  >  2:1627591/1‑150 (MQ=255)

                                                                                                        aGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTc                                           <  2:8876781/150‑1 (MQ=255)

                                                                                                        aGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTc                                           <  1:8200368/150‑1 (MQ=255)

                                                                                                          tAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCATTCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGa                                         <  1:1730547/150‑1 (MQ=255)

                                                                                                          tAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCgc                                         >  2:6319700/1‑149 (MQ=255)

                                                                                                          tAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGa                                         <  2:1769013/150‑1 (MQ=255)

                                                                                                          tAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGa                                         >  2:3818739/1‑150 (MQ=255)

                                                                                                          tAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGa                                         >  1:6267462/1‑150 (MQ=255)

                                                                                                          tAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAAAACCGGACTCTCACGCCGGGGGTCGCGGGTTCGa                                         >  1:5791378/1‑150 (MQ=255)

                                                                                                          tAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTACAATCGGGCGCAAGCCTGAACGATACGAAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGGCGCGGGTTCGa                                         >  1:3810709/1‑150 (MQ=255)

                                                                                                            aCAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGt                                       >  1:3043406/1‑150 (MQ=255)

                                                                                                               gaccggTGCCAGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTccc                                    >  1:6011113/5‑150 (MQ=255)

                                                                                                                 tGGGCGCCTGGCACCGGTCTGCAGCCACCCGGCACATTCCTTGGGCTGATGCCAGTTTCACTCGGGCGCACGGCTGAACGATCCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGt                                  <  2:8831932/150‑1 (MQ=18)

                                                                                                                      gCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCTCGCCGGGGGTCGCGGGTGCGAGTCCCGTCCGCt                             >  2:7373968/1‑150 (MQ=255)

                                                                                                                       ccTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGaa                                                                                                          >  2:4773999/1‑72 (MQ=255)

                                                                                                                        cTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTgc                           >  2:6886394/1‑150 (MQ=255)

                                                                                                                        cTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGCCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTgc                           >  2:8959435/1‑150 (MQ=255)

                                                                                                                        cTGGCACCGGTCTGAAGCCAAAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACTATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTgc                           <  2:2687253/150‑1 (MQ=255)

                                                                                                                         tGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCgg                                                        <  2:2878546/120‑1 (MQ=255)

                                                                                                                         tGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCgg                                                        >  1:2878546/1‑120 (MQ=255)

                                                                                                                         tGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTgcg                          >  2:6703790/1‑150 (MQ=255)

                                                                                                                             aCCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCAt                      >  1:2277324/1‑150 (MQ=255)

                                                                                                                                  tCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCg                 >  1:821827/1‑150 (MQ=255)

                                                                                                                                    tGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGtt               >  2:4241139/1‑150 (MQ=255)

                                                                                                                                    tGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGtt               <  1:2461499/150‑1 (MQ=255)

                                                                                                                                     gAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGttt              <  2:2277324/150‑1 (MQ=255)

                                                                                                                                      aaGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGACAACCGGACTGGCCCGCCGGGGGGACCGGGGACGAGCCCCGACCGCTGCGCCAGCGTCGttga             >  1:1599296/1‑148 (MQ=18)

                                                                                                                                      aaGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGTTTc             >  1:1368354/1‑150 (MQ=255)

                                                                                                                                      aaGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGTTTc             >  1:8661019/1‑150 (MQ=255)

                                                                                                                                      aaGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGTTTc             >  1:5636096/1‑150 (MQ=255)

                                                                                                                                        gCCACCAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGTTTCaa           <  1:4187925/150‑1 (MQ=255)

                                                                                                                                        gCCACAAGGCACATGCCTTTGGCTGATCCCAGTTTGAATCGGTCGCAAGCCTGACCGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGTTTCaa           <  1:1144975/150‑1 (MQ=255)

                                                                                                                                        cccacaagccacaagCCTTGGGCTGATCCCAGATTCAAACGGGCTCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGTTTCaa           <  2:4459173/149‑1 (MQ=17)

                                                                                                                                          cacaAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGTTTCAAgg         >  1:4782023/1‑150 (MQ=255)

                                                                                                                                           acaAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGTTTCAAGGt        >  1:5413121/1‑150 (MQ=255)

                                                                                                                                           acaAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGTTTCAAGGt        >  2:464733/1‑150 (MQ=255)

                                                                                                                                            caAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGTTTCAAGGTc       >  2:1683929/1‑150 (MQ=255)

                                                                                                                                                  acaTGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCgg                                                        >  1:4076594/1‑95 (MQ=255)

AGCCAGAGAGGAACGCACTGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGTTTCAAGGTCCCTAG  >  NC_002947/2273484‑2273778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`