BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,482,784	G→C	6.9%	L176L (CTC→CTG) ‡	PP_2175 ←	3‑oxoacyl‑ACP reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,482,784	0	G	C	6.9%	918.5 / 42.5	275	L176L (CTC→CTG) ‡	PP_2175	3‑oxoacyl‑ACP reductase
Reads supporting (aligned to +/- strand): ref base G (114/142); new base C (10/9); total (124/151)
Fisher's exact test for biased strand distribution p-value = 6.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 291 total aligned reads displayed.

ACCTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCC  >  NC_002947/2482635‑2482933

 ccTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGgga                                                                                                                                                      <  2:7415031/150‑1 (MQ=255)

    tGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTGTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGggagga                                                                                                                                                   <  2:2470439/150‑1 (MQ=255)

     gATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGAc                                                                                                                                                  >  2:5504420/1‑150 (MQ=255)

       tGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACgt                                                                                                                                                >  2:9022060/1‑150 (MQ=255)

         ttCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACgttg                                                                                                                                              <  2:3546943/150‑1 (MQ=255)

          tCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACgttgt                                                                                                                                             <  1:7975294/150‑1 (MQ=255)

          tCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACgttgt                                                                                                                                             <  1:2384446/150‑1 (MQ=255)

            tgacctgTGATGTAGGCCGCACCGTCTGATGCCAGGAAGGCAACTGTTGCGGCAATTTCTTCGCTTTTGCCATAACGCTGCATAGGCACGCTGTCACGGCGCTCTTCAGTGGCTGGCAGGCTGTCGATCCAGCCCGGCAAAACattgttc                                                                                                                                           >  2:1867199/7‑149 (MQ=255)

            tGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGttgttg                                                                                                                                           <  2:2247251/150‑1 (MQ=255)

               cctgTGATGTAGGCCGCACCGTCTGATGCCAGGAAGGCAACTGTTGCGGCAATTTCTTCGCTTTTGCCATAACGCTGCATAGGCACGCTGTCACGGCGCTCTTCAGTGGCTGGCAGGCTGTCGATCCAGCCCGGCAAAACATTGTTCAtg                                                                                                                                        <  1:5075170/147‑2 (MQ=255)

               ccGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATc                                                                                                                                        <  1:3286205/150‑1 (MQ=255)

                 tgTGATGTAGGCCGCACCGTCTGATGCCAGGAAGGCAACTGTTGCGGCAATTTCTTCGCTTTTGCCATAACGCTGCATAGGCACGCTGTCACGGCGCTCTTCAGTGGCTGGCAGGCTGTCGATCCAGCCCGGCAAAACATTGTTCAtgcg                                                                                                                                      >  1:7864353/2‑147 (MQ=255)

                    gATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCAc                                                                                                                                   <  2:6999540/150‑1 (MQ=255)

                        tAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTg                                                                                                                               >  2:7617044/1‑150 (MQ=255)

                         aGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGt                                                                                                                              >  2:3596587/1‑150 (MQ=255)

                         aGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCAGGGAGGACGTTGTTGATCCTCACGTTGt                                                                                                                              >  1:8792700/1‑150 (MQ=255)

                           gCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTcc                                                                                                                            <  2:1552286/150‑1 (MQ=255)

                           gCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTcc                                                                                                                            >  1:969078/1‑150 (MQ=255)

                           gCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTcc                                                                                                                            >  2:7986338/1‑150 (MQ=255)

                                  cTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGa                                                                                                                     >  1:4342451/1‑150 (MQ=255)

                                   ttCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGaa                                                                                                                    <  1:3013125/150‑1 (MQ=255)

                                       cTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTgg                                                                                                                >  1:5288317/1‑150 (MQ=255)

                                        tGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGt                                                                                                               >  2:3758740/1‑150 (MQ=255)

                                         ggCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTc                                                                                                              <  1:2177441/150‑1 (MQ=255)

                                         ggCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTc                                                                                                              <  2:7137756/150‑1 (MQ=255)

                                          gCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCTGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCg                                                                                                             <  2:3373437/150‑1 (MQ=255)

                                           cGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCgg                                                                                                            <  1:5708792/150‑1 (MQ=255)

                                            gagaAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGtt                                                                                                                                >  1:1528274/1‑129 (MQ=255)

                                            gagaAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGc                                                                                                           >  1:154459/1‑150 (MQ=255)

                                            gagaAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGc                                                                                                           >  2:4426354/1‑150 (MQ=255)

                                              gaAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGa                                                                                                         >  1:6469346/1‑150 (MQ=255)

                                              gaAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGa                                                                                                         <  2:8420/150‑1 (MQ=255)

                                                aaCGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAg                                                                                                       <  1:7095584/150‑1 (MQ=255)

                                                 aCGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGa                                                                                                      >  1:2901417/1‑150 (MQ=255)

                                                   gCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGAtt                                                                                                    >  2:3861647/1‑150 (MQ=255)

                                                     aaTGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGAtttt                                                                                                  <  2:6837844/150‑1 (MQ=255)

                                                     aaTGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGAtttt                                                                                                  <  1:1730904/150‑1 (MQ=255)

                                                     aaTGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGAtttt                                                                                                  >  2:5203458/1‑150 (MQ=255)

                                                     aaTGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGAtttt                                                                                                  >  1:3929754/1‑150 (MQ=255)

                                                      aTGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTg                                                                                                 <  1:1955508/150‑1 (MQ=255)

                                                      aTGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTg                                                                                                 <  2:7643770/150‑1 (MQ=255)

                                                      aTGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTg                                                                                                 <  2:4768858/150‑1 (MQ=255)

                                                      aTGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTg                                                                                                 <  1:2724512/150‑1 (MQ=255)

                                                        ggtggCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGt                                                                                               <  1:714260/150‑1 (MQ=255)

                                                        ggtggCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGt                                                                                               <  2:6108805/150‑1 (MQ=255)

                                                        ggtggCGGCGAGTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGt                                                                                               <  2:8724349/150‑1 (MQ=255)

                                                             cggcgATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATg                                                                                          <  1:909661/150‑1 (MQ=255)

                                                              ggcgATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGc                                                                                         >  1:5043580/1‑150 (MQ=255)

                                                               gcgATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCg                                                                                        <  2:6523597/150‑1 (MQ=255)

                                                                cgATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCgg                                                                                       <  2:2597236/150‑1 (MQ=255)

                                                                cgATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCgg                                                                                       >  1:8476395/1‑150 (MQ=255)

                                                                    ttCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAg                                                                                   <  2:4307405/150‑1 (MQ=255)

                                                                    ttCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAg                                                                                   <  2:3000991/150‑1 (MQ=255)

                                                                    ttCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAg                                                                                   >  1:2105597/1‑150 (MQ=255)

                                                                       cTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGcc                                                                                >  2:4468476/1‑150 (MQ=255)

                                                                       cTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGcc                                                                                >  2:324382/1‑150 (MQ=255)

                                                                        tCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGccc                                                                               >  2:1754778/1‑150 (MQ=255)

                                                                          gCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGa                                                                             >  1:1396039/1‑150 (MQ=255)

                                                                            aGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTTATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGc                                                                           <  1:3035920/150‑1 (MQ=255)

                                                                            aGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGc                                                                           <  2:4839651/150‑1 (MQ=255)

                                                                              gTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCgg                                                                         >  1:4793690/1‑150 (MQ=255)

                                                                                   aTAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAacac                                                                    >  1:6836085/1‑150 (MQ=255)

                                                                                    tAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACt                                                                   <  1:85691/150‑1 (MQ=255)

                                                                                    tAGCGTTTGAGAGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACt                                                                   >  2:8155826/1‑150 (MQ=255)

                                                                                       cGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCa                                                                <  1:6573193/150‑1 (MQ=255)

                                                                                         tttGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGa                                                              >  1:3712400/1‑150 (MQ=255)

                                                                                             aGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTc                                                          >  2:7259593/1‑150 (MQ=255)

                                                                                              gCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCg                                                         <  1:7412013/150‑1 (MQ=255)

                                                                                               cGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCgg                                                        <  2:6937975/150‑1 (MQ=255)

                                                                                               cGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCgg                                                        <  2:2860684/150‑1 (MQ=255)

                                                                                                ggCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCggg                                                       >  2:4969652/1‑150 (MQ=255)

                                                                                                ggCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCggg                                                       >  1:7633693/1‑150 (MQ=255)

                                                                                                 acACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGa                                                      >  2:5188777/2‑150 (MQ=255)

                                                                                                  cACGCTGTCACGGCGCTCTTCAGTGGCTGGCAGGCTGTCGATCCAGCCCGGCAAAACATTGTTCATGCGAATGTTGTCGGGTGCATAAGTGTCGGCAAATACCTTCGTGAAAGAGGCGAGGCCTGCGCGGAACACCGCCGAAGTCGGGaa                                                     <  1:7808634/150‑1 (MQ=255)

                                                                                                    cGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACa                                                   <  2:6036721/150‑1 (MQ=255)

                                                                                                           gcggcgCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACt                                            <  2:4503137/150‑1 (MQ=255)

                                                                                                           gcggcgCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACt                                            <  1:4596633/150‑1 (MQ=255)

                                                                                                            cggcgCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTg                                           <  2:8530269/150‑1 (MQ=255)

                                                                                                             ggcgCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTgg                                          >  2:5283725/1‑150 (MQ=255)

                                                                                                                    ttCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGaa                                   <  1:3758740/150‑1 (MQ=255)

                                                                                                                     tCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAAc                                  <  2:7904238/150‑1 (MQ=255)

                                                                                                                       ggTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGc                                >  2:1400282/1‑150 (MQ=255)

                                                                                                                         tggctggcAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGccc                              >  1:2218209/1‑150 (MQ=255)

                                                                                                                             tggcAGGCTGTCGATCCAGCCGGTGAGGACGTTGTTGATCCTCACTTTGTCCCCGGCGATTTGGTCGGCGAAGATTTTGTTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGc                          <  1:3985027/150‑1 (MQ=255)

                                                                                                                             tggcAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGc                          >  2:4236226/1‑150 (MQ=255)

                                                                                                                                   gCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGa                    >  1:3622925/1‑150 (MQ=255)

                                                                                                                                      gTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAAt                 <  2:4643187/150‑1 (MQ=255)

                                                                                                                                        cGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGt               <  2:4111366/150‑1 (MQ=255)

                                                                                                                                        cGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGt               <  1:1086999/150‑1 (MQ=255)

                                                                                                                                         gATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGtt              <  1:8941210/150‑1 (MQ=255)

                                                                                                                                         gATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGtt              <  2:5828157/150‑1 (MQ=255)

                                                                                                                                          aTCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTtg             <  2:2074868/150‑1 (MQ=255)

                                                                                                                                           tCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTtga            <  2:301804/150‑1 (MQ=255)

                                                                                                                                           tCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTtga            <  1:2890641/150‑1 (MQ=255)

                                                                                                                                           tCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTtga            <  2:8833952/150‑1 (MQ=255)

                                                                                                                                           tCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTtga            <  2:1107857/150‑1 (MQ=255)

                                                                                                                                            ccAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTtgat           <  1:4081653/150‑1 (MQ=255)

                                                                                                                                                 cGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCa      <  1:7048982/150‑1 (MQ=255)

                                                                                                                                                  ggggAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCAc     <  2:637876/150‑1 (MQ=255)

                                                                                                                                                    ggaggaCGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGc   >  2:2703648/1‑150 (MQ=255)

ACCTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACGCCCAGGCGGTGGAAATGTTGATGATCACGCC  >  NC_002947/2482635‑2482933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`