Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,675,147 A→C 7.1% M166L (ATG→CTG)  PP_2340 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,675,1470AC 7.1% 672.2 / 26.4 247M166L (ATG→CTG) PP_2340hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (111/106);  new base C (7/13);  total (118/129)
Fisher's exact test for biased strand distribution p-value = 2.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 259 total aligned reads displayed.

GTGACCTGGAGGCCTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCACTTGGCGATCACTTCGCTGG  >  NC_002947/2674998‑2675296
                                                                                                                                                     |                                                                                                                                                     
gTGACCTGGAGGCCTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCa                                                                                                                                                       >  2:3239681/1‑150 (MQ=255)
 tGACCTGGAGGCCTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTAACATCGCTATATCGACCCCAGCTTCCTGCGCGATGCCAt                                                                                                                                                      >  1:1896538/1‑150 (MQ=255)
  gACCTGGAGGCCTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAAGTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATg                                                                                                                                                     <  2:8014464/150‑1 (MQ=255)
   aCCTGGAGGCCTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATgg                                                                                                                                                    >  2:1502314/1‑150 (MQ=255)
      tGGAGGCCTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCa                                                                                                                                                 <  2:4152886/150‑1 (MQ=255)
      tGGAGGCCTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCa                                                                                                                                                 <  1:3842320/150‑1 (MQ=255)
      tGGAGGCCTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCa                                                                                                                                                 <  1:363282/150‑1 (MQ=255)
            ccTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAgcgcgc                                                                                                                                           <  1:6394867/150‑1 (MQ=255)
             cTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGCTGCCCATGGCATCGCGCa                                                                                                                                          <  1:7983006/150‑1 (MQ=255)
             cTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCa                                                                                                                                          >  1:8060324/1‑150 (MQ=255)
             cTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCa                                                                                                                                          <  1:7808660/150‑1 (MQ=255)
                 ggCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAggga                                                                                                                                      <  2:1482184/150‑2 (MQ=255)
                    aTGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAg                                                                                                                                   >  2:2488962/1‑150 (MQ=255)
                     tGGTGTTGCTGCGGTTGTTCGGTGACTGGTAAGGGCCCCATTTGCGCGATGCGATCTTTGGCGAGTTCTTCCTGATTTCAGCGGCGCACTACCAGCGATATATCGCCCCCAGCTTCCTGCGCGTTGCCTTGGGCAGCGCGCAGGGCCAGt                                                                                                                                  <  1:4679084/150‑1 (MQ=255)
                     tGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGt                                                                                                                                  >  2:2311036/1‑150 (MQ=255)
                       gtgtTGCTGCCGGTGTTCGGTGACTGGGAACTGCCCCAATTGCGCGTTGCGATCGATCGCGTGTTCTTCCTGCTTTCAGAGGCGCACTACCCGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGa                                                                                                                                <  1:2156956/150‑1 (MQ=255)
                       gtgtTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGa                                                                                                                                <  2:2722289/150‑1 (MQ=255)
                       gtgtTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGa                                                                                                                                <  1:6796176/150‑1 (MQ=255)
                               gcCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCg                                                                                                                        >  2:5162299/1‑150 (MQ=255)
                                ccGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGa                                                                                                                       >  2:386366/1‑150 (MQ=255)
                                 cGGAGTTCCGTGACTGGTAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGaa                                                                                                                      <  2:8581897/150‑1 (MQ=255)
                                   gAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATc                                                                                                                    >  1:2875459/1‑150 (MQ=255)
                                      ttCCTTGAGTGGGAACTGCCCCAATTGCGCTATGCGATGGAGCGAGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCGTCCTGCGCGATGCAATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGt                                                                                                                 <  2:1891189/150‑1 (MQ=255)
                                      ttCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGt                                                                                                                 <  2:7735618/150‑1 (MQ=255)
                                      ttCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGt                                                                                                                 >  2:6359304/1‑150 (MQ=255)
                                        ccGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGACTCAGTCg                                                                                                               >  1:5457908/1‑150 (MQ=255)
                                        ccGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCg                                                                                                               >  2:4553120/1‑150 (MQ=255)
                                               tGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGgtgt                                                                                                        >  1:2009391/1‑150 (MQ=255)
                                               tGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGgtgt                                                                                                        >  1:3540137/1‑150 (MQ=255)
                                               tGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGgtgt                                                                                                        >  1:7469012/1‑150 (MQ=255)
                                               tGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGgtgt                                                                                                        >  1:6113547/1‑150 (MQ=255)
                                                   aaCTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATAGCGCCCCCCGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTc                                                                                                    <  1:7316971/150‑1 (MQ=255)
                                                   aaCTGCCCCAATTGCGCGATGCGATCGAGCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTc                                                                                                    <  1:5952248/150‑1 (MQ=255)
                                                       gCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCaa                                                                                                >  2:330077/1‑150 (MQ=255)
                                                        ccccAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAAc                                                                                               >  1:120425/1‑150 (MQ=255)
                                                         cccAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCCGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAAcc                                                                                              >  1:3796863/1‑150 (MQ=255)
                                                          ccAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCg                                                                                             >  2:3516673/1‑150 (MQ=255)
                                                           cAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGc                                                                                            >  1:8228262/1‑150 (MQ=255)
                                                                gcgcGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTg                                                                                       <  2:494934/150‑1 (MQ=255)
                                                                   cgatgcgatCGAGCGCGCTTCCTACCTGCTTTCCGAGGCGCACACCCAGCGCTAAAGCGCCCCCCGCTGCCGGCGCGTTGCCATGGCCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAg                                                                                    <  1:87589/150‑1 (MQ=255)
                                                                    gatgcgatCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAgg                                                                                   >  1:7905096/1‑150 (MQ=255)
                                                                      tgcgatCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGtt                                                                                 <  1:2972503/150‑1 (MQ=255)
                                                                          atcgatcgCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGt                                                                             >  2:2025915/1‑150 (MQ=255)
                                                                          atcgatcgCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGt                                                                             <  2:9101899/150‑1 (MQ=255)
                                                                          atcgatcgCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGt                                                                             <  2:3644437/150‑1 (MQ=255)
                                                                          atcgatcgCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGt                                                                             <  2:6481963/150‑1 (MQ=255)
                                                                          atcgatcgCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGt                                                                             <  2:7927817/150‑1 (MQ=255)
                                                                          atcgatcgCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGt                                                                             <  2:1465931/150‑1 (MQ=255)
                                                                          atcgatcgCGAGTTCTTCCTGCTTGCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGAGGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGt                                                                             <  2:5388349/150‑1 (MQ=255)
                                                                             gttcgCGATTGCTTCCTGCTTTCATCGGCGCACTCCCAGCGCTACATCGCCCCCAGCTTCGTGCGCTTTGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGa                                                                          <  1:101049/148‑1 (MQ=255)
                                                                              atcgCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGACTCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGAt                                                                         <  1:720802/150‑1 (MQ=255)
                                                                              atcgCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGAt                                                                         >  1:1542080/1‑150 (MQ=255)
                                                                               tcgCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATg                                                                        >  1:2190933/1‑150 (MQ=255)
                                                                                  cgAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGa                                                                     >  1:2732126/1‑150 (MQ=255)
                                                                                    aGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAAc                                                                   <  2:4398654/150‑1 (MQ=255)
                                                                                    aGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGAGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAAc                                                                   <  2:5619493/150‑1 (MQ=255)
                                                                                       tcttcCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCtt                                                                <  1:6720027/150‑1 (MQ=255)
                                                                                       tcttcCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCtt                                                                <  2:596967/150‑1 (MQ=255)
                                                                                        cttcCTGCTTTCAGAGGCGCACTACCTGCGCTATATCGCCCCCCGCTTCGTGCGCGCTGCCATGGGCTGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTg                                                               <  1:4835116/150‑1 (MQ=255)
                                                                                        cttcCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTg                                                               <  1:8267931/150‑1 (MQ=255)
                                                                                        cttcCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCGGCGCGTGGCCTTGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTg                                                               <  1:265566/150‑1 (MQ=255)
                                                                                         ttcCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCCGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTgg                                                              >  1:1795134/1‑150 (MQ=255)
                                                                                         ttcCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTgg                                                              >  2:8386002/1‑150 (MQ=255)
                                                                                          tcCTGCTTTCAGCGGCGCACTACCAGCGCTATAGCGCCCCCAGCTTCCTGCGCGATGCCAGGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGa                                                             <  2:3148330/150‑1 (MQ=255)
                                                                                          tcCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGa                                                             >  2:4370559/1‑150 (MQ=255)
                                                                                          tcCTGCTTGCCGAGGCGCACTCCCCGCGCTATAGCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGa                                                             <  1:4962127/150‑1 (MQ=255)
                                                                                          tcCTGATTTCAGAGGCGCACTACCAGCGATATATCCCCCCCAGCTACCTGCGCTATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGa                                                             <  1:946392/150‑1 (MQ=255)
                                                                                           ccTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGAc                                                            <  1:6325127/150‑1 (MQ=255)
                                                                                            cTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACa                                                           >  2:2938666/1‑150 (MQ=255)
                                                                                            cTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACa                                                           >  2:5245056/1‑150 (MQ=255)
                                                                                             tGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGa                                                             >  1:4424422/1‑147 (MQ=255)
                                                                                                 ttCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTcc                                                      <  1:5327839/150‑1 (MQ=255)
                                                                                                  tCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCg                                                     >  2:1521897/1‑150 (MQ=255)
                                                                                                   cAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCgg                                                    >  2:3566870/1‑150 (MQ=255)
                                                                                                          gcACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGc                                             >  1:3337308/1‑150 (MQ=255)
                                                                                                          gcACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGc                                             >  2:102614/1‑150 (MQ=255)
                                                                                                             ctcccAGCGCTATATCGCCCCCCGCTGCCGGCGCGCTGCCCGGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGc                                          <  1:4618409/147‑1 (MQ=255)
                                                                                                                ccAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTg                                       >  1:8905918/1‑150 (MQ=255)
                                                                                                                  aGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGAc                                     >  2:4812568/1‑150 (MQ=255)
                                                                                                                   gcgcTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGAcc                                    <  2:6832215/150‑1 (MQ=255)
                                                                                                                      cTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTgg                                 <  2:1630640/150‑1 (MQ=255)
                                                                                                                       tataTCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGt                                >  1:3012626/1‑150 (MQ=255)
                                                                                                                         tatCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTcc                              <  2:6305499/150‑1 (MQ=255)
                                                                                                                          atCGCCCCCAGCTTCCTGCGCTATGCCTTGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTccc                             <  1:418311/150‑1 (MQ=255)
                                                                                                                           tCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCa                            <  2:8427240/150‑1 (MQ=255)
                                                                                                                               ccccAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATc                        <  2:4479096/150‑1 (MQ=255)
                                                                                                                               ccccAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATc                        >  2:3256646/1‑150 (MQ=255)
                                                                                                                               ccccAGCTTCCTGCGCGAGGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATc                        <  1:7767589/150‑1 (MQ=255)
                                                                                                                                  cAGCTTCCTGCGCGATGCCTGGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCAc                     <  1:1807106/150‑1 (MQ=255)
                                                                                                                                  cAGCTTCCTGCGCGATGCCCTGGGCCGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGGCTTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCAc                     >  2:3763699/1‑150 (MQ=255)
                                                                                                                                      ttCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCACTTgg                 >  2:8924271/1‑150 (MQ=255)
                                                                                                                                       cccTGCGCGCTGCCCATGGCATCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGt                                                                             >  1:8547385/2‑89 (MQ=37)
                                                                                                                                         cTGCGCGCTGCCCATGGCATCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCACTTGGCGa              >  2:677549/1‑150 (MQ=255)
                                                                                                                                          tGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCACTTGGCGAt             >  1:7048911/1‑150 (MQ=255)
                                                                                                                                          tGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCACTTGGCGAt             >  2:5292603/1‑150 (MQ=255)
                                                                                                                                           gcgcGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTg                                                               >  2:2750939/1‑99 (MQ=255)
                                                                                                                                                    cATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCACTTGGCGATCACTTCGCtg   <  2:2190751/150‑1 (MQ=255)
                                                                                                                                                     aTGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCACTTGGCGATCACTTCGCtgg  >  2:6496682/1‑150 (MQ=255)
                                                                                                                                                     aTGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCACTTGGCGATCACTTCGCtgg  >  1:5524383/1‑150 (MQ=255)
                                                                                                                                                     aTGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCACTTGGCGATCACTTCGCtgg  >  2:709449/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTGACCTGGAGGCCTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCGATCGCGAGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCACTTGGCGATCACTTCGCTGG  >  NC_002947/2674998‑2675296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑