BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	14,887	C→A	5.3%	L520M (CTG→ATG)	gyrB →	DNA gyrase subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	14,887	0	C	A	5.3%	929.2 / 25.1	274	L520M (CTG→ATG)	gyrB	DNA gyrase subunit B
Reads supporting (aligned to +/- strand): ref base C (139/120); new base A (8/7); total (147/127)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 277 total aligned reads displayed.

GCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGCCTGCAC  >  NC_002947/14738‑15036

 cACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCt                                                                                                                                                      <  2:3863366/150‑1 (MQ=255)

    gCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGcc                                                                                                                                                   >  1:754299/1‑150 (MQ=255)

       gATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGa                                                                                                                                                <  2:8253458/150‑1 (MQ=255)

       gATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGa                                                                                                                                                >  1:4302789/1‑150 (MQ=255)

            cTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTgg                                                                                                                                           <  1:1703313/150‑1 (MQ=255)

            cTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTgg                                                                                                                                           >  2:5313370/1‑150 (MQ=255)

               cgcTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTc                                                                                                                                         <  2:5855778/149‑1 (MQ=255)

                gcTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGa                                                                                                                                       <  1:204404/150‑1 (MQ=255)

                      cTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTgg                                                                                                                                 <  1:4914488/150‑1 (MQ=255)

                      cTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTgg                                                                                                                                 <  2:4224900/150‑1 (MQ=255)

                       tgtgGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTCCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGc                                                                                                                                >  2:1105445/1‑150 (MQ=255)

                         tgGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTa                                                                                                                              >  2:6179323/1‑150 (MQ=255)

                          ggCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTAc                                                                                                                             <  2:1181980/150‑1 (MQ=255)

                          ggCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTAc                                                                                                                             <  2:1923065/150‑1 (MQ=255)

                              tCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCt                                                                                                                         <  2:6391846/150‑1 (MQ=255)

                                ggCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCtat                                                                                                                       <  2:6290788/150‑1 (MQ=255)

                                    gcgAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTg                                                                                                                   <  2:2447884/150‑1 (MQ=255)

                                     cgAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGc                                                                                                                  >  1:6039209/1‑150 (MQ=255)

                                      gAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGcc                                                                                                                 <  2:6484062/150‑1 (MQ=255)

                                            tACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAgccg                                                                                                           >  2:6911471/1‑150 (MQ=255)

                                            tACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAgccg                                                                                                           >  2:2483280/1‑150 (MQ=255)

                                               aatcctgACAAGCTGCGCTACCATAAGATCATCATCATGACGGATGCTGACGTCGACGGTGCGCATATTCGTACGCTGCTGTTGACCTTCTTCTTCCGTCAGATGCCGGAACTGATCGAGCGTGGCCATATCTATATCGCGCAGccgccg                                                                                                        <  2:8056282/144‑1 (MQ=255)

                                               aacaTCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGccgccg                                                                                                        >  1:5566960/1‑150 (MQ=255)

                                                acaTCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCcgccgc                                                                                                       <  1:6816554/150‑1 (MQ=255)

                                                 tcctgACAAGCTGCGCTACCATAAGATCATCATCATGACGGATGCTGACGTCGACGGTGCGCATATTCGTACGCTGCTGTTGACCTTCTTCTTCCGTCAGATGCCGGAACTGATCGAGCGTGGCCATATCTATATCGCGCAGCCGCCGCt                                                                                                      >  1:2850627/5‑150 (MQ=255)

                                                 caTCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCt                                                                                                      <  1:5275486/150‑1 (MQ=255)

                                                  aTCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTg                                                                                                     >  2:3142616/1‑150 (MQ=255)

                                                     gACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTAc                                                                                                  <  1:2856331/150‑1 (MQ=255)

                                                       cAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACaa                                                                                                <  2:5213467/150‑1 (MQ=255)

                                                       cAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACaa                                                                                                <  1:144965/150‑1 (MQ=255)

                                                       cAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACaa                                                                                                <  2:7263319/150‑1 (MQ=255)

                                                         aaCTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAgg                                                                                              <  2:6816451/150‑1 (MQ=255)

                                                         aaCTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAgg                                                                                              <  2:4048133/150‑1 (MQ=255)

                                                          gcTGCGCTACCATAAGATCATCATCATGACGGATGCTGACGTCGACGGTGCGCATATTCGTACGCTGCTGTTGACCTTCTTCTTCCGTCAGATGCCGGAACTGATCGAGCGTGGCCATATCTATATCGCGCAGCCGCCGCTCTATAAGGt                                                                                             >  2:4212119/2‑150 (MQ=255)

                                                          gcTGCGCTACCATAAGATCATCATCATGACGGATGCTGACGTCGACGGTGCGCATATTCGTACGCTGCTGTTGACCTTCTTCTTCCGTCAGATGCCGGAACTGATCGAGCGTGGCCATATCTATATCGCGCAGCCGCCGCTCTATAAGGt                                                                                             >  1:7631197/2‑150 (MQ=255)

                                                              cGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGaaa                                                                                         <  2:863151/150‑1 (MQ=255)

                                                              cGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGaaa                                                                                         <  1:7171441/150‑1 (MQ=255)

                                                               gatATCACAACATCAAAATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCAGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAAc                                                                                        >  2:2260637/3‑150 (MQ=255)

                                                               gTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAAc                                                                                        >  1:6389747/1‑150 (MQ=255)

                                                                 tATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGccgccg                                                                                                        <  1:3697209/132‑1 (MQ=255)

                                                                 tATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGa                                                                                      <  1:8407378/150‑1 (MQ=255)

                                                                  aTCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTTTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAg                                                                                     <  1:1883845/150‑1 (MQ=255)

                                                                    cacaACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGc                                                                                   <  1:1265703/150‑1 (MQ=255)

                                                                     acaacaTCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCa                                                                                  >  2:1395459/1‑150 (MQ=255)

                                                                     acaacaTCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCa                                                                                  >  2:8522073/1‑150 (MQ=255)

                                                                      caacaTCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGAGCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCaa                                                                                 >  1:3800824/1‑150 (MQ=255)

                                                                      caacaTCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCaa                                                                                 >  2:2964054/1‑150 (MQ=255)

                                                                       aacaTCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAg                                                                                >  1:7373952/1‑150 (MQ=255)

                                                                         catcatcatcatGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCa                                                                              >  2:7826326/1‑150 (MQ=255)

                                                                              tcatcatGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGc                                                                         >  2:3948284/1‑150 (MQ=255)

                                                                               catcatGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCa                                                                        <  1:6200877/150‑1 (MQ=255)

                                                                               catcatGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCa                                                                        <  2:4523111/150‑1 (MQ=255)

                                                                                   atGGCCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTAc                                                                    >  1:4446642/1‑150 (MQ=255)

                                                                                   atGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGccc                                                                                                                <  2:1571445/106‑1 (MQ=255)

                                                                                   atGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTAc                                                                    >  1:4551221/1‑150 (MQ=255)

                                                                                      aCCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATc                                                                 >  2:5542971/1‑150 (MQ=255)

                                                                                              tGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGa                                                         >  1:2809312/1‑150 (MQ=255)

                                                                                              tGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGa                                                         >  1:8006767/1‑150 (MQ=255)

                                                                                               gACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGAt                                                        >  2:3409119/1‑150 (MQ=255)

                                                                                                aCGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATg                                                       >  2:1746793/1‑150 (MQ=255)

                                                                                                 cGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGa                                                      <  2:3670686/150‑1 (MQ=255)

                                                                                                 cGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGa                                                      <  2:4348980/150‑1 (MQ=255)

                                                                                                     gACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGcc                                                  >  1:3070595/1‑150 (MQ=255)

                                                                                                     gACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGcc                                                  >  2:4093057/1‑150 (MQ=255)

                                                                                                        ggTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATg                                               <  2:8010877/150‑1 (MQ=255)

                                                                                                        ggTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATg                                               <  2:7871589/150‑1 (MQ=255)

                                                                                                        ggTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATg                                               <  2:5637692/150‑1 (MQ=255)

                                                                                                           tCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGaa                                            <  1:549321/150‑1 (MQ=255)

                                                                                                             gCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAaga                                          >  2:5372558/1‑150 (MQ=255)

                                                                                                             gCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAaga                                          <  1:3279120/150‑1 (MQ=255)

                                                                                                             gCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAAGACGATGAGGCCATGGAaga                                          >  2:4838791/1‑150 (MQ=255)

                                                                                                              cacaTCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAagag                                         >  2:5857303/1‑150 (MQ=255)

                                                                                                                 aTCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTAc                                      <  1:8253571/150‑1 (MQ=255)

                                                                                                                 aTCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTAc                                      >  1:5971216/1‑150 (MQ=255)

                                                                                                                  tCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACa                                     <  1:8680996/150‑1 (MQ=255)

                                                                                                                  tCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACa                                     <  1:5857303/150‑1 (MQ=255)

                                                                                                                     gTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGa                                  <  1:8136991/150‑1 (MQ=255)

                                                                                                                     gTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGa                                  >  1:4609390/1‑150 (MQ=255)

                                                                                                                      tACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGAc                                 >  2:1869314/1‑150 (MQ=255)

                                                                                                                      tACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGAc                                 <  1:3470967/150‑1 (MQ=255)

                                                                                                                         gctgctgctGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCa                              >  1:7939056/1‑150 (MQ=255)

                                                                                                                           tgctgctgACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGt                            >  1:663900/1‑150 (MQ=255)

                                                                                                                            gctgctgACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTc                           <  1:3148033/150‑1 (MQ=255)

                                                                                                                             ctgctgACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCg                          >  1:6093649/1‑150 (MQ=255)

                                                                                                                                 tgACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGctc                      <  2:2883471/150‑1 (MQ=255)

                                                                                                                                 tgACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGctc                      >  1:9100389/1‑150 (MQ=255)

                                                                                                                                    ccTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTgg                   >  2:8817557/1‑150 (MQ=255)

                                                                                                                                     cttcttcttCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGa                  >  2:1428794/1‑150 (MQ=255)

                                                                                                                                     cttcttcttCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGa                  >  1:1001717/1‑150 (MQ=255)

                                                                                                                                     cttcttcttCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGa                  >  1:893758/1‑150 (MQ=255)

                                                                                                                                      ttcttcttcCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGTGGCAAGAAGGAGCAGTACATCATGGACGATGATGAGAGGGAAGAGTACATGACCCAGTCGGCTCTGGaa                 >  1:504463/1‑150 (MQ=255)

                                                                                                                                      ttcttcttcCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGaa                 >  2:5430664/1‑150 (MQ=255)

                                                                                                                                         ttcttcCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGAt              >  1:3983335/1‑150 (MQ=255)

                                                                                                                                          agctcCGGCAGCTGACGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATg             >  1:2311412/4‑150 (MQ=255)

                                                                                                                                            ttcCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGcc           >  1:3386222/1‑150 (MQ=255)

                                                                                                                                            ttcCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGcc           >  1:6786138/1‑150 (MQ=255)

                                                                                                                                            ttcCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGcc           >  2:8149474/1‑150 (MQ=255)

                                                                                                                                             tcCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCa          <  1:4032189/150‑1 (MQ=255)

                                                                                                                                                   aGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGCCTGc    >  1:7797621/1‑150 (MQ=255)

                                                                                                                                                     cTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGCCTGCAc  <  2:2035271/150‑1 (MQ=255)

GCACGCTGATCACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGCCTGCAC  >  NC_002947/14738‑15036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`