Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,035,510 A→T 5.1% D29V (GAC→GTC)  PP_2646 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,035,5100AT 5.1% 951.2 / 26.7 285D29V (GAC→GTC) PP_2646hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (148/121);  new base T (12/3);  total (161/124)
Fisher's exact test for biased strand distribution p-value = 6.53e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01

Only 100 of 300 total aligned reads displayed.

CTGCGTGGTCGCCCAGGCCATGAAGACCCTCTATATCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATGGCGCCGATCTGGTGCGTTACCCGCCAAACTGGCAGCTGCACGGCAAGCAGGCCGAACGC  >  NC_002947/3035361‑3035658
                                                                                                                                                     |                                                                                                                                                     
 tGCGTGGTCGCCCAGGCCATGAAGACCCTCTATATCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGgacgac                                                                                                                                                      >  1:6667571/1‑150 (MQ=255)
 tGCGTGGTCGCCCAGGCCATGAAGACCCTCTATATCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGgacgac                                                                                                                                                      >  2:3386179/1‑150 (MQ=255)
 tGCGTGGTCGCCCAGGCCATGAAGACCCTCTATATCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGgacgac                                                                                                                                                      <  1:4044495/150‑1 (MQ=255)
    gTGGTCGCCCAGGCCATGAAGACCCTCTATATCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTc                                                                                                                                                   <  1:8078045/150‑1 (MQ=255)
    gTGGTCGCCCAGGCCATGAAGACCCTCTATATCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTc                                                                                                                                                   <  1:3648751/150‑1 (MQ=255)
          gCCCAGGCCATGAAGACCCTCTATATCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCCGGCCCGGGACGACGGcccgcgc                                                                                                                                             >  1:3900471/1‑150 (MQ=255)
          gCCCAGGCCATGAAGACCCTCTATATCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTccagcgc                                                                                                                                             >  2:7781342/1‑150 (MQ=255)
          gCCCAGGCCATGAAGACCCTCTATATCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTccagcgc                                                                                                                                             >  2:5400735/1‑150 (MQ=255)
          gCCCAGGCCATGAAGACCCTCTATATCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTccagcgc                                                                                                                                             >  1:4062230/1‑150 (MQ=255)
            ccAGGCCATGAAGACCCTCTATATCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTccagcgcc‑a                                                                                                                                          >  2:8295003/1‑150 (MQ=255)
             cAGGCCATGAAGACCCTCTATATCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTccagcgcc‑ag                                                                                                                                         >  2:8392593/1‑150 (MQ=255)
               ggCCATGAAGACCCTCTATATCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTacagcgcc‑agcg                                                                                                                                       >  1:771088/1‑150 (MQ=255)
                 ccATGAAGACCCTCTATATCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGc                                                                                                                                     <  1:8521817/150‑1 (MQ=255)
                  cATGAAGACCCTCTATATCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGcc                                                                                                                                    >  1:8361601/1‑150 (MQ=255)
                     gAAGACCCTCTATATCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGAt                                                                                                                                 >  1:5106446/1‑150 (MQ=255)
                       aGACCCTCTATATCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATcc                                                                                                                               <  2:3941149/150‑1 (MQ=255)
                       aGACCCTCTATATCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATcc                                                                                                                               <  2:8544677/150‑1 (MQ=255)
                         aCCCTCTATATCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCgg                                                                                                                             <  1:2071191/150‑1 (MQ=255)
                           ccTCTATATCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGgt                                                                                                                           <  2:7379706/150‑1 (MQ=255)
                           ccTCTATATCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGgt                                                                                                                           <  2:478780/150‑1 (MQ=255)
                            ctctATATCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑CGCGGCCGATCCGGgtg                                                                                                                          >  1:6076183/1‑150 (MQ=255)
                            ctctATATCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑ACCGGCCGAGCCGGgtg                                                                                                                          >  1:1054738/1‑150 (MQ=255)
                             tctATATCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGgtgt                                                                                                                         >  2:1986004/1‑150 (MQ=255)
                             tctATATCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGgtgt                                                                                                                         >  2:6858630/1‑150 (MQ=255)
                             tctATATCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGgtgt                                                                                                                         >  1:2192955/1‑150 (MQ=255)
                              ctATATCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGATCGGCCG‑CTGGCGCTGGACGTCGTCCAGGGCCTGGCGGCAGCGCCGGgtgtc                                                                                                                        <  2:9119630/150‑2 (MQ=18)
                               tataTCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTg                                                                                                                       <  2:8486282/150‑1 (MQ=255)
                                     cgccgTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCCGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCAc                                                                                                                 >  1:3288947/1‑150 (MQ=255)
                                     cgccgTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACAATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCAGGACGACTACAAGCCCC‑ACCGGCCGATCCGGGTGTTGATCCAc                                                                                                                 >  1:8775094/1‑150 (MQ=255)
                                      gccgTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACg                                                                                                                >  1:7478313/1‑150 (MQ=255)
                                      gccgTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACg                                                                                                                >  2:5108878/1‑150 (MQ=255)
                                      gccgTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACg                                                                                                                >  2:634806/1‑150 (MQ=255)
                                          tttCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑CGCGGCCGATCCGGGTGTTGATCCACGGTgg                                                                                                            >  1:9158131/1‑150 (MQ=255)
                                             cGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCAGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAg                                                                                                         >  1:4269704/1‑150 (MQ=255)
                                              ggCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGc                                                                                                        >  2:8955529/1‑150 (MQ=255)
                                               gCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGcc                                                                                                       >  1:275706/1‑150 (MQ=255)
                                                ccAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCa                                                                                                      >  2:8201014/1‑150 (MQ=255)
                                                  aGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCAGt                                                                                                    >  2:8814711/1‑150 (MQ=255)
                                                    tGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCAGttt                                                                                                  <  1:616077/150‑1 (MQ=255)
                                                    tGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCAGttt                                                                                                  <  1:6765136/150‑1 (MQ=255)
                                                        tCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTtg                                                                                              <  1:8719727/150‑1 (MQ=255)
                                                          tGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTtggt                                                                                            >  2:7022259/1‑150 (MQ=255)
                                                            aGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCgg                                                                                                                                      >  2:827310/1‑106 (MQ=255)
                                                            aGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTtggtgg                                                                                          >  2:8324589/1‑150 (MQ=255)
                                                             gCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTggg                                                                                         <  1:3484510/150‑1 (MQ=255)
                                                             gCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTACAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTggg                                                                                         <  1:645377/150‑1 (MQ=255)
                                                                  gCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAAt                                                                                    <  2:2875656/150‑1 (MQ=255)
                                                                    aaGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCa                                                                                                      <  2:5959140/130‑1 (MQ=255)
                                                                    aaGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCa                                                                                                      >  1:5959140/1‑130 (MQ=255)
                                                                    aaGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCg                                                                                  >  1:1659600/1‑150 (MQ=255)
                                                                    aaGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCAGGACCACGTCCAGCGCC‑CGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCg                                                                                  >  1:2600269/1‑150 (MQ=255)
                                                                        agagCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAg                                                                              <  1:4988195/150‑1 (MQ=255)
                                                                            cAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACTGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTgg                                                                          >  2:3779963/1‑150 (MQ=255)
                                                                              gACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCACGCCCAGGACCACGTCCAGCCCC‑CGCGGCCGATACCGGTGTTGATACACCGTGGCCGCCCGGTTATTTGTGGGGAAAACCAAAGCAGGgc                                                                        >  1:4012230/1‑150 (MQ=255)
                                                                               aCCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTTATACACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGgcg                                                                       >  1:4373842/1‑150 (MQ=255)
                                                                                 cATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGgcgcg                                                                     >  1:4541531/1‑150 (MQ=255)
                                                                                  aTGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCCCCAGGCCCAGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACCGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGgcgcgc                                                                    >  1:7929091/1‑150 (MQ=255)
                                                                                    gCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGa                                                                  >  2:4089029/1‑150 (MQ=255)
                                                                                      gAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGc                                                                >  2:8636440/1‑150 (MQ=255)
                                                                                       aGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCa                                                               <  1:944295/150‑1 (MQ=255)
                                                                                            tGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATGgcg                                                          >  2:1499647/1‑150 (MQ=255)
                                                                                              atatGTGCAGGGCGCGACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATGGCGcc                                                        <  1:8970205/150‑1 (MQ=255)
                                                                                                  gtgCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATGGCGCCGATc                                                    >  2:2578483/1‑150 (MQ=255)
                                                                                                     cAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATGGCGCCGATCTgg                                                 >  2:1205248/1‑150 (MQ=255)
                                                                                                      aGGGCGCACCTCCGCCGACACCCGGCGCTGCCGCCAGGCCCTGGGCGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCCCGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATGGCGCCGATCTGGt                                                <  2:5841167/150‑1 (MQ=255)
                                                                                                      aGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATGGCGCCGATCTGGt                                                <  2:2480962/150‑1 (MQ=255)
                                                                                                           gcAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCGGGACGACGTCCACCGCC‑CGCGGCCCATCCGGGTGTTGATCCACGGGGGCAGCCAGGTTCTTGGTGGGGAAATAGAAAGCTGGGCGCGCGAGCATGGCGCCGATCTGGTGCGtt                                           >  1:1844680/1‑150 (MQ=255)
                                                                                                             aaCTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATGGCGCCGATCTGGTGCGTTAc                                         >  1:7416295/1‑150 (MQ=255)
                                                                                                                tggatcaacacCCGGATCGGCCG‑CTGGCGCTGGACGTCGTCCAGGGCCTGGCGGCAGCGCCGGGTGTTGATCCACGGTGGCAGCCAGGTTCTTGGTGGGGAAATCGAAAGCGGGGCGCGCGAGCCTGGCGCCGATCTGGTGCGTTACCCg                                      >  1:1251913/8‑150 (MQ=12)
                                                                                                                   gCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATGGCGCCGATCTGGTGCGTTACCCGCCa                                   >  2:7262501/1‑150 (MQ=255)
                                                                                                                   gCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATGGCGCCGATCTGGTGCGTTACCCGCCa                                   <  2:4493852/150‑1 (MQ=255)
                                                                                                                    tcaacacCCGGATCGGCCG‑CTGGCGCTGGACGTCGTCCAGGGCCTGGCGGCAGCGCCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATGGCGCCGATCTGGTGCGTTACCCGCCaa                                  >  1:5649447/4‑150 (MQ=18)
                                                                                                                      aacacCCGGATCGGCCG‑CTGGCGCTGGACGTCGTCCAGGGCCTGGCGGCAGCGCCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCTCGCGAGCATGGCGCCGATCTGGTGCGTTACCCGCCAAAc                                >  1:4341992/2‑150 (MQ=18)
                                                                                                                       acacCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGGGGGGAAATCGAAAGCTGGGCGCGCGAGCATGGCGCCGATCTGGTGCGTTACCCGCCAAACt                               >  1:8201885/1‑150 (MQ=255)
                                                                                                                       acacCCGGATCGGCCG‑CTGGCGCTGGACGTCGTCCAGGGCCTGGCGGCAGCGCCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATGGCGCCGATCTGGTGCGTTACCCGCCAAACt                               >  2:133731/1‑150 (MQ=21)
                                                                                                                         acCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATGGCGCCGATCTGGTGCGTTACCCGCCAAACTgg                             <  2:3643965/150‑1 (MQ=255)
                                                                                                                         acCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATGGCGCCGATCTGGTGCGTTACCCGCCAAACTgg                             <  1:5251461/150‑1 (MQ=255)
                                                                                                                          cccGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATGGCGCCGATCTGGTGCGTTACCCGCCAAACTGGc                            <  1:3577770/150‑1 (MQ=255)
                                                                                                                           ccGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCCTGGCGCCGATCTGGTGCGTTACCCGCCAAACTGGCa                           >  1:6938204/1‑150 (MQ=255)
                                                                                                                           ccGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATGGCGCCGATCTGGTGCGTTACCCGCCAAACTGGCa                           <  2:2928539/150‑1 (MQ=255)
                                                                                                                           ccGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATGGCGCCGATCTGGTGCGTTACCCGCCAAACTGGCa                           >  1:5122703/1‑150 (MQ=255)
                                                                                                                           ccGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTGGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATGGCGCCGATCTGGGGCGTTACCCGCCAAACTGGcc                           >  2:5661810/1‑149 (MQ=255)
                                                                                                                             ggCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATGGCGCCGATCTGGTGCGTTACCCGCCAAACTGGCAGc                         <  2:1591029/150‑1 (MQ=255)
                                                                                                                                  tGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATGGCGCCGATCTGGTGCGTTACCCGCCAAACTGGCAGCTGCAc                    >  1:7043548/1‑150 (MQ=255)
                                                                                                                                   gccgccAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATGGCGCCGATCTGGTGCGTTCCCCGCCAAACTGGCAGCTGCACg                   >  2:8717656/1‑150 (MQ=255)
                                                                                                                                   gccgccAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATGGCGCCGATCTGGTGCGTTACCCGCCAAACTGGCAGCTGCACg                   <  1:175208/150‑1 (MQ=255)
                                                                                                                                   gccgccAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATGGCGCCGATCTGGTGCGTTACCCGCCAAACTGGCAGCTGCACg                   <  1:6870242/150‑1 (MQ=255)
                                                                                                                                     cgccAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATGGCGCCGATCTGGTGCGTTACCCGCCAAACTGGCAGCTGCACGGc                 <  1:4705695/150‑1 (MQ=255)
                                                                                                                                     cgccAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCAGGTGCTTGGTGGGGAACTCGAAAGCTGGGCGCGCGAGCATGGCGCCGATCTGGTGCGTTACCCGCCCAACTGGCAGCTGCACGGc                 >  2:3139965/1‑150 (MQ=255)
                                                                                                                                     cgacAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATGGCGCCGATCTGGTGCGTTACCCGCCAAACTGGCAGCTGCACGGc                 <  2:7675828/147‑1 (MQ=255)
                                                                                                                                     ccgctggCGCTGGACGTCGTCCAGGGCCTGGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATGGCGCCGATCTGGTGCGTTACCCGCCAAACTGGCAGCTGCACgg                  >  1:7812708/6‑150 (MQ=38)
                                                                                                                                      gccAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATGGCGCCGATCTGGTGCGTTACCCGCCAAACTGGCAGCTGCACGGCa                >  1:2516056/1‑150 (MQ=255)
                                                                                                                                      gccAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATGGCGCCGATCTGGTGCGTTACCCGCCAAACTGGCAGCTGCACGGCa                >  2:6640076/1‑150 (MQ=255)
                                                                                                                                      gccAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATGGCGCCGATCTGGTGCGTTACCCGCCAAACTGGCAGCTGCACGGCa                >  1:1448617/1‑150 (MQ=255)
                                                                                                                                           gCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATGGCGCCGATCTGGTGCGTTACCCGCCAAACTGGCAGCTGCACGGCAAGCAg           <  2:6563733/150‑1 (MQ=255)
                                                                                                                                             ccTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATGGCGCCGATCTGGTGCGTTACCCGCCAAACTGGCAGCTGCACGGCAAGCAGGc         <  2:3288947/150‑1 (MQ=255)
                                                                                                                                              cTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATGGCGCCGATCTGGTGCGTTACCCGCCAAACTGGCAGCTGCACGGCAAGCAGGcc        >  1:5383858/1‑150 (MQ=255)
                                                                                                                                                 gacgacGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATGGCGCCGATCTGGTGCGTTACCCGCCAAACTGGCAGCTGCACGGCAAGCAGGCCGaa     <  2:7573099/150‑1 (MQ=255)
                                                                                                                                                   cgacgTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATGGCGCCGATCTGGTGCGTTACCCGCCAAACTGGCAGCTGCACGGCAAGCAGGCCGAACg   >  2:3952507/1‑150 (MQ=255)
                                                                                                                                                    gacgTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATGGCGCCGATCTGGTGCGTTACCCGCCAAACTGGCAGCTGCACGGCAAGCAGGCCGAACGc  >  1:4858984/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTGCGTGGTCGCCCAGGCCATGAAGACCCTCTATATCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGGACGACGTCCAGCGCC‑AGCGGCCGATCCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATGGCGCCGATCTGGTGCGTTACCCGCCAAACTGGCAGCTGCACGGCAAGCAGGCCGAACGC  >  NC_002947/3035361‑3035658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑