BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,294,286	G→C	8.2%	Q535H (CAG→CAC)	PP_2893 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,294,286	0	G	C	8.2%	907.7 / 17.5	305	Q535H (CAG→CAC)	PP_2893	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (118/157); new base C (27/3); total (145/160)
Fisher's exact test for biased strand distribution p-value = 4.25e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 308 total aligned reads displayed.

ATGCTCAAGGCCGAGACCCAGGGGGCAGGGCAAAACTGGACCCTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGA  >  NC_002947/3294137‑3294435

    tCAAGGCCGAGACCCAGGGGGCAGGGCAAAACTGGACCCTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCtgt                                                                                                                                                    >  2:8839550/1‑150 (MQ=255)

          ccGAGACCCAGGGGGCAGGGCAAAACTGGACCCTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGa                                                                                                                                              <  1:3684543/150‑1 (MQ=255)

           cGAGACCCAGGGGGCAGGGCAAAACTGGACCCTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATTGACCTTGACTTGCCGCGCCTCGGCCACAGCTGGCCGAg                                                                                                                                             <  1:8910603/150‑1 (MQ=255)

           cGAGACCCAGGGGGCAGGGCAAAACTGGACCCTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAg                                                                                                                                             >  1:6582203/1‑150 (MQ=255)

            gagaCCCAGGGGGCAGGGCAAAACTGGACCCTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAgg                                                                                                                                            <  2:4228715/150‑1 (MQ=255)

            gagaCCCAGGGGGCAGGGCAAAACTGGACCCTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAgg                                                                                                                                            <  1:4416105/150‑1 (MQ=255)

            gagaCCCAGGGGGCAGGGCAAAACTGGACCCTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAgg                                                                                                                                            <  1:8545815/150‑1 (MQ=255)

                 ccAGGGGGCAGGGCAAAACTGGACCCTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACa                                                                                                                                       >  2:7048901/1‑150 (MQ=255)

                  cAGGGGGCAGGGCAAAACTGGACCCTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACaa                                                                                                                                      <  2:570375/150‑1 (MQ=255)

                  cAGGGGGCAGGGCAAAACTGGACCCTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCGCGGCCAGCGGTGGCCGCGGCTACaa                                                                                                                                      >  2:6185437/1‑150 (MQ=255)

                   aGGGGGCAGGGCAAAACTGGACCCTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAg                                                                                                                                     >  2:7194918/1‑150 (MQ=255)

                          agggcaAAACTGGACCCTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGt                                                                                                                              <  2:8046256/150‑1 (MQ=255)

                            ggcaAAACTGGACCCTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCa                                                                                                                            >  2:6316948/1‑150 (MQ=255)

                            ggcaAAACTGGACCCTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCGCGGCCAGCGGTGGCCGAGGGGACAAGGCCggggga                                                                                                                            >  1:4900013/1‑147 (MQ=255)

                             gcaAAACTGGTCCCTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCATCGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCaa                                                                                                                           <  2:1771215/150‑1 (MQ=255)

                               aaaaCTGGACCCTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAgg                                                                                                                         <  2:6260509/150‑1 (MQ=255)

                               aaaaCTGGACCCTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCGCGGCCCCCGGTGGGCGAGGCGACAAGGCCGGGGCAAgg                                                                                                                         >  1:3299658/1‑150 (MQ=255)

                                aatcTGGACGCTGGGCACACGGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGACTCGTCCAGCTGTGGACGAGGGTACAAGGCCGGGTCAAgtt                                                                                                                        <  2:5345150/147‑3 (MQ=255)

                                aaaCTGGACCCTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCGGTGGCCGAGGCTACAATGCCGGGTCAAggg                                                                                                                        >  2:638916/1‑150 (MQ=255)

                                 aaCTGGACCCTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGc                                                                                                                       >  2:3512448/1‑150 (MQ=255)

                                 aaCTGGACCCTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGc                                                                                                                       >  2:7255169/1‑150 (MQ=255)

                                    tGGACCCTGGGCACACTGGCCATCCAGCTGGGTGACACCCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCgg                                                                                                                    <  1:5871557/150‑1 (MQ=255)

                                    tGGACCCTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCgg                                                                                                                    <  2:1030500/150‑1 (MQ=255)

                                     ggACCCTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGc                                                                                                                   <  1:445153/150‑1 (MQ=255)

                                         ccTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCCCCTGGGGCCGACGGGACAACGCCCGGGAAAAGGCCCGCGGGa                                                                                                               >  1:4602276/1‑150 (MQ=255)

                                         ccTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGa                                                                                                               <  2:8398391/150‑1 (MQ=255)

                                          cTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCTGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGAt                                                                                                              <  1:6137716/150‑1 (MQ=255)

                                          cTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGAt                                                                                                              <  1:1534580/150‑1 (MQ=255)

                                          cTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGAt                                                                                                              <  1:5746415/150‑1 (MQ=255)

                                          cTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCGCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGAt                                                                                                              >  2:4905760/1‑150 (MQ=255)

                                           tGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATg                                                                                                             >  2:2436909/1‑150 (MQ=255)

                                             ggCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCCCCTGTTGCCGAGGCTACAAGGCCCGGTCAAGGGCCGGCGGGATGTc                                                                                                           >  1:4717000/1‑150 (MQ=255)

                                             ggCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTc                                                                                                           <  2:4402631/150‑1 (MQ=255)

                                                 cacTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCg                                                                                                       <  1:831929/150‑1 (MQ=255)

                                                 cacTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCCCGGCCCCCGGTGGCCCAGGCGACAAGGCCCGGGCAACGGCCGGCGGGGTGTCGCCg                                                                                                       >  1:3165572/1‑150 (MQ=255)

                                                    tGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCa                                                                                                    >  1:8185549/1‑150 (MQ=255)

                                                    tGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCGCGGCCCGCGGTGGCCGCGGCTACAAGGCCCGGGCAAGGGCCCGCGGGGTGGGGCCGGcc                                                                                                    >  1:1781486/1‑149 (MQ=255)

                                                     ggCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCAc                                                                                                   >  2:5095387/1‑150 (MQ=255)

                                                      gCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGTTGTCGCCGGCAcc                                                                                                  >  2:2927517/1‑150 (MQ=255)

                                                       ccATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCt                                                                                                 >  2:1152014/1‑150 (MQ=255)

                                                         ctCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTg                                                                                               <  1:7431664/149‑1 (MQ=255)

                                                          tCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGc                                                                                              <  1:9128740/150‑1 (MQ=255)

                                                           ccAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCa                                                                                             >  2:7588982/1‑150 (MQ=255)

                                                            cAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAg                                                                                            >  2:1497830/1‑150 (MQ=255)

                                                             aGCTGGGTGACAACCGCATCACCGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCGGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGcccg                                                                                           <  2:2040853/150‑4 (MQ=255)

                                                                tGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGccc                                                                                        >  2:5803758/1‑150 (MQ=255)

                                                                tGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGccc                                                                                        <  1:25107/150‑1 (MQ=255)

                                                                tGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCGCGGCCAGCGGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCATGCAGGccc                                                                                        >  2:899841/1‑150 (MQ=255)

                                                                      aCAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCGCGGCCAGCAGTGGCCCAGGCGAAAAAGCCGGGGCAATGGCCGGCGGGGTGTCGCCCGCCCCATTCAGGCCCCCCAAg                                                                                  >  1:3212986/1‑150 (MQ=255)

                                                                          ccGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTAc                                                                              >  2:7402511/1‑150 (MQ=255)

                                                                          ccGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTAc                                                                              >  2:5784472/1‑150 (MQ=255)

                                                                              aTCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCCGGTCAAGGGCCGGCGGGATGTCGCCGGCACCTTTCAGGCCCCCCAAGGTACGCTc                                                                          >  2:3226478/1‑150 (MQ=255)

                                                                                 aaCGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGGCTCGGCCACCGGTGGCCGGGGCGACAACGCCGGGGGCACGGCCGGCTGGATGTCGCCGGCCCCAAGCAGGCCCCCCCAGGGACGCGCAcc                                                                       >  1:2625802/1‑150 (MQ=255)

                                                                                 aaCGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCAcc                                                                       <  2:3420335/150‑1 (MQ=255)

                                                                                  aCGGCAGTGACAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCAccc                                                                      >  2:3704376/1‑150 (MQ=255)

                                                                                      cAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCGCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCCAGGTACGCTCACCCAGCa                                                                  >  2:1639751/1‑150 (MQ=255)

                                                                                        gTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAgg                                                                <  1:3416942/150‑1 (MQ=255)

                                                                                         tGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAggg                                                               <  1:6413435/150‑1 (MQ=255)

                                                                                         tGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCGCGGCCAGCTGTGGCCGCGGGTACAAGGCCGGGGCCACGGCCGGCGGGATGTCGCCGGCACCTTGCAGGCCCCCCCAGGTACCCGCACCCAGCAggg                                                               >  1:7987917/1‑150 (MQ=255)

                                                                                           gCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGcc                                                             >  2:8665302/1‑150 (MQ=255)

                                                                                             aGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑Ag                                                          <  1:7639581/150‑1 (MQ=255)

                                                                                             aGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑Ag                                                          <  2:1879128/150‑1 (MQ=255)

                                                                                             aGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑Ag                                                          <  2:8212458/150‑1 (MQ=255)

                                                                                              gCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCGCGGCCCCCGGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCCGCACCGTGCAGGCCCCGCCCGGTACGCGCACCCAGCAGGGCC‑Agc                                                         >  2:240422/1‑150 (MQ=255)

                                                                                               cTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCCCCGTGCAGGCCCCGCAAGGTACGCTCACCCGGCAGGGCC‑Agcg                                                        >  2:1375539/1‑150 (MQ=255)

                                                                                               cTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTGGCCCTGCAGGGTG‑Agcg                                                        <  2:8479720/150‑1 (MQ=255)

                                                                                                ttGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCTGGCCCTGCAGGGTGAGCGTACCTTGCGGGGCC‑Tgcaa                                                       <  1:6316948/150‑3 (MQ=37)

                                                                                                ttGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCACGGCCAGCTGTGGCCGAGGCTACAATGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑Agcgc                                                       >  1:9015190/1‑150 (MQ=255)

                                                                                                  gCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCtt                                                     <  1:3945978/150‑1 (MQ=255)

                                                                                                   cAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCGCGGCCCGCTGTGGCCCAGGCGACCAGGCCGGGGCAAGGGCCGGCGGGATGTCGCCGGCCCCATGCCGGCCCCGCCAGGTACGCGCACCCAGCCGGGCC‑AGCGCGTg                                                    >  1:3633320/1‑150 (MQ=255)

                                                                                                      cAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGcc                                                 <  1:1020898/150‑1 (MQ=255)

                                                                                                           ccTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCCGCTGTGGCCGAGGCTAAAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCAACATGCAGGCCCCGCAAGGTACGCTCACCCAGCAGGGCC‑AGCGCTTGGCCCAGGc                                            >  2:4746890/1‑150 (MQ=255)

                                                                                                            ctggctggGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCCAAGCGC‑TGGccctgcag                                           <  1:8839550/150‑6 (MQ=255)

                                                                                                                   ggCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAAcc                                    <  1:3034260/150‑1 (MQ=255)

                                                                                                                    gCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCg                                   <  2:3144539/150‑1 (MQ=255)

                                                                                                                     cGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGc                                  <  1:7013011/150‑1 (MQ=255)

                                                                                                                     cGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGc                                  <  1:558999/150‑1 (MQ=255)

                                                                                                                          cGACCTGGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTgca                             <  2:6809467/150‑1 (MQ=255)

                                                                                                                           gACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCAGCAGGGCC‑AGCGCTTGGCCCAGGCCGCAAACCGCCTgcag                            >  2:2155944/1‑150 (MQ=255)

                                                                                                                            aCCTTGACTTGCCGCGCCGCGGCCACCGGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCAGGTTGTCGCCGGCACCATTCAGGCCCCCCAAGGTACGCTCACCCAGCAGGGCC‑AGCGCTTGGCCCAGGCCCAAAACCGCCAgccgc                           >  1:2908968/1‑150 (MQ=255)

                                                                                                                               ttGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGt                        <  2:6579200/150‑1 (MQ=255)

                                                                                                                                 gACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTg                      <  2:7746464/150‑1 (MQ=255)

                                                                                                                                   cTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGa                    <  2:5755471/150‑1 (MQ=255)

                                                                                                                                    ttGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGAc                   <  2:3959437/150‑1 (MQ=255)

                                                                                                                                    ttGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGAc                   <  1:8678495/150‑1 (MQ=255)

                                                                                                                                     tGCCGCGCCTCGGCCAGCTGTTGCCGAGGCTACAAGGCCGGGTCAAGGTCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGAcc                  <  1:2365987/150‑1 (MQ=255)

                                                                                                                                      ttgtagcCTCGGCCACAGCTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCt                 >  1:7277750/6‑150 (MQ=255)

                                                                                                                                      gCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCt                 <  1:5061565/150‑1 (MQ=255)

                                                                                                                                       ccGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTg                <  1:7174823/150‑1 (MQ=255)

                                                                                                                                       ccGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTg                <  2:8600864/150‑1 (MQ=255)

                                                                                                                                        cgcgCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTgg               >  1:1950644/1‑150 (MQ=255)

                                                                                                                                          cgcCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGAt             <  2:5616436/150‑1 (MQ=255)

                                                                                                                                            ccTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGc           <  1:1440984/150‑1 (MQ=255)

                                                                                                                                            ccTCGGCCACAGCTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGc           >  2:8294288/1‑150 (MQ=255)

                                                                                                                                                 gCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGcc      >  1:8065811/1‑150 (MQ=255)

                                                                                                                                                 gCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGcc      >  1:8243085/1‑150 (MQ=255)

                                                                                                                                                  ccAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCt     >  2:2633308/1‑150 (MQ=255)

                                                                                                                                                   cAGCTGTGGCCGAGGCTACACGGCCGGGTCAGGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCCCCTg    <  1:1750719/150‑1 (MQ=255)

                                                                                                                                                     gCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGa  <  1:6847667/150‑1 (MQ=255)

                                                                                                                                                     gCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGa  <  2:4045971/150‑1 (MQ=255)

ATGCTCAAGGCCGAGACCCAGGGGGCAGGGCAAAACTGGACCCTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGA  >  NC_002947/3294137‑3294435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`