BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,294,288	T→G	11.9%	L536R (CTG→CGG)	PP_2893 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,294,288	0	T	G	11.9%	743.0 / 19.3	298	L536R (CTG→CGG)	PP_2893	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (88/156); new base G (40/3); total (139/159)
Fisher's exact test for biased strand distribution p-value = 1.08e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 310 total aligned reads displayed.

GCTCAAGGCCGAGACCCAGGGGGCAGGGCAAAACTGGACCCTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACA  >  NC_002947/3294139‑3294437

  tCAAGGCCGAGACCCAGGGGGCAGGGCAAAACTGGACCCTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCtgt                                                                                                                                                      >  2:8839550/1‑150 (MQ=255)

        ccGAGACCCAGGGGGCAGGGCAAAACTGGACCCTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGa                                                                                                                                                <  1:3684543/150‑1 (MQ=255)

         cGAGACCCAGGGGGCAGGGCAAAACTGGACCCTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATTGACCTTGACTTGCCGCGCCTCGGCCACAGCTGGCCGAg                                                                                                                                               <  1:8910603/150‑1 (MQ=255)

         cGAGACCCAGGGGGCAGGGCAAAACTGGACCCTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAg                                                                                                                                               >  1:6582203/1‑150 (MQ=255)

          gagaCCCAGGGGGCAGGGCAAAACTGGACCCTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAgg                                                                                                                                              <  1:8545815/150‑1 (MQ=255)

          gagaCCCAGGGGGCAGGGCAAAACTGGACCCTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAgg                                                                                                                                              <  1:4416105/150‑1 (MQ=255)

          gagaCCCAGGGGGCAGGGCAAAACTGGACCCTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAgg                                                                                                                                              <  1:2382063/150‑1 (MQ=255)

               ccAGGGGGCATGGCAAAACTGGACCCTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACa                                                                                                                                         >  2:7491176/1‑150 (MQ=255)

               ccAGGGGGCAGGGCAAAACTGGACCCTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACa                                                                                                                                         >  2:7048901/1‑150 (MQ=255)

                cAGGGGGCAGGGCAAAACTGGACCCTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACaa                                                                                                                                        <  2:570320/150‑1 (MQ=255)

                 aGGGGGCAGGGCAAAACTGGACCCTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAg                                                                                                                                       >  2:7194918/1‑150 (MQ=255)

                     ggcagggcaAAACTGGACCCTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCg                                                                                                                                   <  1:8143002/150‑1 (MQ=255)

                        agggcaAAACTGGACCCTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGt                                                                                                                                <  2:8046256/150‑1 (MQ=255)

                          ggcaAAACTGGACCCTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCa                                                                                                                              >  2:6316948/1‑150 (MQ=255)

                          ggcaAAACTGGACCCTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCGCGGCCAGCGGTGGCCGAGGGGACAAGGCCggggga                                                                                                                              >  1:4900013/1‑147 (MQ=255)

                           gcaAAACTGGTCCCTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCATCGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCaa                                                                                                                             <  2:1771215/150‑1 (MQ=255)

                            caAAACTGGACCCTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAg                                                                                                                            <  2:4199695/150‑1 (MQ=255)

                            caAAACTGGACCCTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAg                                                                                                                            <  1:4647613/150‑1 (MQ=255)

                             aaaaCTGGACCCTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAgg                                                                                                                           <  2:6260509/150‑1 (MQ=255)

                             aaaaCTGGACCCTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCGCGGCCCCCGGTGGGCGAGGCGACAAGGCCGGGGCAAgg                                                                                                                           >  1:3299658/1‑150 (MQ=255)

                              aatcTGGACGCTGGGCACACGGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGACTCGTCCAGCTGTGGACGAGGGTACAAGGCCGGGTCAAgtt                                                                                                                          <  2:5345150/147‑3 (MQ=255)

                              aaaCTGGACCCTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCGGTGGCCGAGGCTACAATGCCGGGTCAAggg                                                                                                                          >  2:638916/1‑150 (MQ=255)

                               aaCTGGACCCTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGc                                                                                                                         >  2:7255169/1‑150 (MQ=255)

                                aCTGGACCCTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGcc                                                                                                                        <  1:2517455/150‑1 (MQ=255)

                                aCTCGACCCTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCGGGGCCACCGGTGGCCCCGGCGACAACGCCGGGTCAAggggc                                                                                                                        >  1:3224633/1‑148 (MQ=255)

                                  tGGACCCTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCgg                                                                                                                      <  2:1030500/150‑1 (MQ=255)

                                   ggACCCTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGc                                                                                                                     <  1:445153/150‑1 (MQ=255)

                                        cTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGAt                                                                                                                <  1:1534580/150‑1 (MQ=255)

                                        cTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCGCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGAt                                                                                                                >  2:4905760/1‑150 (MQ=255)

                                         tGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATg                                                                                                               >  2:2436909/1‑150 (MQ=255)

                                           ggCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGGCACGGCCAGCAGTGGCCGAGGCGACAAGGCCCGGTCAAGGGCCGGCGGGATGTc                                                                                                             >  1:4717474/1‑150 (MQ=255)

                                               cacTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCg                                                                                                         <  1:831929/150‑1 (MQ=255)

                                                acTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCGCGGCCCCCGGTGGCCGAGGCGACAATGCCGGGTCAAGGGCCGGCGGGGTGTCGCCgg                                                                                                        >  1:2051445/1‑150 (MQ=255)

                                                   ggCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCAc                                                                                                     >  2:5095387/1‑150 (MQ=255)

                                                    gCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGTTGTCGCCGGCAcc                                                                                                    >  2:2927517/1‑150 (MQ=255)

                                                     ccATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCt                                                                                                   >  2:1152014/1‑150 (MQ=255)

                                                     ccATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCGACAAGGCCGGGTCAAGGGCCGGCGGGATGGCGCCGGCCCCt                                                                                                   >  2:5741260/1‑150 (MQ=255)

                                                       ctCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTg                                                                                                 <  1:7431664/149‑1 (MQ=255)

                                                        tCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGc                                                                                                <  1:9128740/150‑1 (MQ=255)

                                                          cAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAg                                                                                              >  2:1497830/1‑150 (MQ=255)

                                                           aGCTGGGTGACAACCGCATCACCGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCGGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGcccg                                                                                             <  2:2040853/150‑4 (MQ=255)

                                                            gCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCGCGGGCCCCAGTGGCCCCGGCGCCCACGCCGGGGCAAGGGCCGGCGGGGGGGCGCCCGCCCCCTGCAGGc                                                                                            >  1:1751856/1‑150 (MQ=255)

                                                              tGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGccc                                                                                          >  2:5803396/1‑150 (MQ=255)

                                                              tGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCGCGGCCAGCGGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCATGCAGGccc                                                                                          >  2:899841/1‑150 (MQ=255)

                                                                        ccGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGGCAGCTGTGGCCGAGGCTACAATGCCGGGTCAAGGGCCGGGTGGATGTGGCCGGCACCTTGCAGGGCCCGCAAGGTAc                                                                                >  2:7402070/1‑150 (MQ=255)

                                                                            aTCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCCGGTCAAGGGCCGGCGGGATGTCGCCGGCACCTTTCAGGCCCCCCAAGGTACGCTc                                                                            >  2:3226478/1‑150 (MQ=255)

                                                                               aaCGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCAcc                                                                         <  2:4011059/150‑1 (MQ=255)

                                                                               aaCGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCGGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCAcc                                                                         >  2:3615289/1‑150 (MQ=255)

                                                                                aCGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCAccc                                                                        >  2:577876/1‑150 (MQ=255)

                                                                                    cAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCGCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCCAGGTACGCTCACCCAGCa                                                                    >  2:1639751/1‑150 (MQ=255)

                                                                                      gTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAgg                                                                  <  1:3416942/150‑1 (MQ=255)

                                                                                       tGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCGCGGCCAGCTGTGGCCGCGGGTACAAGGCCGGGGCCACGGCCGGCGGGATGTCGCCGGCACCTTGCAGGCCCCCCCAGGTACCCGCACCCAGCAggg                                                                 >  1:7987917/1‑150 (MQ=255)

                                                                                         gCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGcc                                                               >  2:8665302/1‑150 (MQ=255)

                                                                                          cAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCGCGGCCCGCTGTGGCCGAGGCTACCAGGCCGGGTGAAGGGCCGGCGGGGTGTCGCCGGCCCCTTGCAGGCCCCCCAAGGTACGCGCACCCAGCAGGGccc                                                              >  1:5864963/1‑149 (MQ=255)

                                                                                           aGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑Ag                                                            <  1:7639581/150‑1 (MQ=255)

                                                                                           aGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑Ag                                                            <  2:1879128/150‑1 (MQ=255)

                                                                                           aGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑Ag                                                            <  2:8212458/150‑1 (MQ=255)

                                                                                            gCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCGCGGCCCCCGGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCCGCACCGTGCAGGCCCCGCCCGGTACGCGCACCCAGCAGGGCC‑Agc                                                           >  2:240422/1‑150 (MQ=255)

                                                                                             cTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCCCCGTGCAGGCCCCGCAAGGTACGCTCACCCGGCAGGGCC‑Agcg                                                          >  2:1375539/1‑150 (MQ=255)

                                                                                              ttGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCACGGCCAGCTGTGGCCGAGGCTACAATGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑Agcgc                                                         >  1:9015190/1‑150 (MQ=255)

                                                                                                gCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCtt                                                       <  1:3945978/150‑1 (MQ=255)

                                                                                                gCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCCCAAGGTACGCGCACCCTGCAGGGCC‑AGCGCtt                                                       >  1:6106008/1‑150 (MQ=255)

                                                                                                 cAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCGCGGCCCGCTGTGGCCCAGGCGACCAGGCCGGGGCAAGGGCCGGCGGGATGTCGCCGGCCCCATGCCGGCCCCGCCAGGTACGCGCACCCAGCCGGGCC‑AGCGCGTg                                                      >  1:3633320/1‑150 (MQ=255)

                                                                                                  aaCAGCGCCTGGCTGGGCGAAGCGCCCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCACGGTACGCCCACCCTGCAGGGCC‑AGCGCTTgg                                                     <  1:6407611/150‑1 (MQ=255)

                                                                                                   aCAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCGGTGGCCGCGGCGACAAGGCCGGGGCAAGGGCCGGCGGGCTGGCGCCGGCCCCGTGCAGGCCCCCCCACGGACGCGCCCCCCGCCGGGCC‑CCCGCGTGGc                                                    >  2:3393306/1‑150 (MQ=255)

                                                                                                    cAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGcc                                                   <  1:1020898/150‑1 (MQ=255)

                                                                                                         ccTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCCGCTGTGGCCGAGGCTAAAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCAACATGCAGGCCCCGCAAGGTACGCTCACCCAGCAGGGCC‑AGCGCTTGGCCCAGGc                                              >  2:4746890/1‑150 (MQ=255)

                                                                                                          ctggctggGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCCAAGCGC‑TGGccctgcag                                             <  1:8839550/150‑6 (MQ=255)

                                                                                                                 ggCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAAcc                                      <  1:3034270/150‑1 (MQ=255)

                                                                                                                 ggCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAAcc                                      <  2:8325298/150‑1 (MQ=255)

                                                                                                                  gCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCg                                     <  2:3144539/150‑1 (MQ=255)

                                                                                                                  gCGAATCGACCTTGACTTGCCGCGCCACGGCCACCTGTGGCCCAGGCGACAAGGCCGGGGCAACGGCCGGCGGGCTGGCGCCGGCACCTTGCAGGCCCCCCCACGTCCGCTCCCCCAGCCGGGCC‑CGCGCGTGGCCCAGGCCGAAAAccc                                     >  2:344323/1‑149 (MQ=255)

                                                                                                                   cGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGc                                    <  1:7013011/150‑1 (MQ=255)

                                                                                                                   cGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGc                                    <  1:558999/150‑1 (MQ=255)

                                                                                                                        cGACCTGGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTgca                               <  2:6809467/150‑1 (MQ=255)

                                                                                                                         gACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCAGCAGGGCC‑AGCGCTTGGCCCAGGCCGCAAACCGCCTgcag                              >  2:2155944/1‑150 (MQ=255)

                                                                                                                         gACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAACGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAACACCGCCTgcag                              <  1:3201575/150‑1 (MQ=255)

                                                                                                                          aCCTTGACTTGCCGCGCCGCGGCCACCGGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCAGGTTGTCGCCGGCACCATTCAGGCCCCCCAAGGTACGCTCACCCAGCAGGGCC‑AGCGCTTGGCCCAGGCCCAAAACCGCCAgccgc                             >  1:2908968/1‑150 (MQ=255)

                                                                                                                             ttGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGt                          <  1:5741260/150‑1 (MQ=255)

                                                                                                                             ttGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGt                          <  2:6579200/150‑1 (MQ=255)

                                                                                                                               gACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTg                        <  2:7746464/150‑1 (MQ=255)

                                                                                                                                  ttGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGAc                     <  1:8678495/150‑1 (MQ=255)

                                                                                                                                    ttgtagcCTCGGCCACAGCTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCt                   >  1:7277750/6‑150 (MQ=255)

                                                                                                                                    gCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCt                   <  1:5061565/150‑1 (MQ=255)

                                                                                                                                     ccGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTg                  <  1:7174823/150‑1 (MQ=255)

                                                                                                                                     ccGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTg                  <  2:8600864/150‑1 (MQ=255)

                                                                                                                                     ccGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTg                  <  2:3893579/150‑1 (MQ=255)

                                                                                                                                        cgcCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGAt               <  2:5616436/150‑1 (MQ=255)

                                                                                                                                          ccTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGc             <  1:1853033/150‑1 (MQ=255)

                                                                                                                                          ccTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGc             <  1:1440984/150‑1 (MQ=255)

                                                                                                                                          ccTCGGCCACAGCTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGc             >  2:8294288/1‑150 (MQ=255)

                                                                                                                                          ccTCGGCCACAGCTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGc             >  2:8422493/1‑150 (MQ=255)

                                                                                                                                              ggCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGc         <  1:6182020/150‑1 (MQ=255)

                                                                                                                                               gCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGcc        >  1:8065584/1‑150 (MQ=255)

                                                                                                                                               gCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGcc        >  1:8243085/1‑150 (MQ=255)

                                                                                                                                                ccAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCt       >  2:2633308/1‑150 (MQ=255)

                                                                                                                                                 cAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTg      <  2:425622/150‑1 (MQ=255)

                                                                                                                                                  aGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTgg     >  2:6128132/1‑150 (MQ=255)

                                                                                                                                                   gCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGa    <  1:6847667/150‑1 (MQ=255)

                                                                                                                                                     tgtgGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCt                                  >  1:2666261/1‑118 (MQ=255)

GCTCAAGGCCGAGACCCAGGGGGCAGGGCAAAACTGGACCCTGGGCACACTGGCCATCCAGCTGGGTGACAACCGCATCAACGGCAGTGGCAGCTTGCAACAGCGCCTGGCTGGGCGAATCGACCTTGACTTGCCGCGCCTCGGCCAGCTGTGGCCGAGGCTACAAGGCCGGGTCAAGGGCCGGCTGGATGTCGCCGGCACCTTGCAGGCCCCGCAAGGTACGCTCACCCTGCAGGGCC‑AGCGCTTGGCCCAGGCCGAAAACCGCCTGCAGCAGTTGGACCTGGATGCCCGCCTGGACA  >  NC_002947/3294139‑3294437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`