Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,296,317 G→T 5.0% L1212L (CTG→CTT)  PP_2893 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,296,3170GT 5.0% 745.5 / 19.0 219L1212L (CTG→CTT) PP_2893hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (89/119);  new base T (5/6);  total (94/125)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 222 total aligned reads displayed.

TCTGGCAACAGCACCAGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGGGACGATCAGGTTGGTGTA  >  NC_002947/3296168‑3296465
                                                                                                                                                     |                                                                                                                                                    
  tGGCAACAGCACCAGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGc                                                                                                                                                    >  1:2190573/1‑150 (MQ=255)
    gCAACAGCACCAGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCaa                                                                                                                                                  >  1:4693004/1‑150 (MQ=255)
    gCAACAGCACCAGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCaa                                                                                                                                                  >  2:7816814/1‑150 (MQ=255)
     cAACAGCACCAGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAg                                                                                                                                                 <  2:920037/150‑1 (MQ=255)
      aaCAGCACCAGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGc                                                                                                                                                <  1:5954751/150‑1 (MQ=255)
      aaCAGCACCAGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGc                                                                                                                                                >  1:7336579/1‑150 (MQ=255)
         aGCACCAGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGAGCTTGCCAGCCCa                                                                                                                                             <  1:410205/150‑1 (MQ=255)
          gCACCAGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCAc                                                                                                                                            >  1:9110990/1‑150 (MQ=255)
           cACCCGCGTGGTCGCCAGCGGGCCCAGCCCCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGCACCGGCCACCACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACt                                                                                                                                           <  2:629420/150‑1 (MQ=255)
           cACCAGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACt                                                                                                                                           <  2:8856346/150‑1 (MQ=255)
            aCCAGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTg                                                                                                                                          <  1:8787449/150‑1 (MQ=255)
            aCCAGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTg                                                                                                                                          <  1:2674014/150‑1 (MQ=255)
             cccgCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGTGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTgg                                                                                                                                         <  2:7505992/147‑1 (MQ=255)
              cAGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGAGCTTGCCAGCCCACTggc                                                                                                                                        <  2:4065351/150‑2 (MQ=255)
              cAGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGAGCTTGCCAGCCCACTggc                                                                                                                                        <  1:9054521/150‑2 (MQ=255)
               aGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGAc                                                                                                                                       >  2:94686/1‑150 (MQ=255)
                   tGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACAtct                                                                                                                                   <  1:7816814/150‑1 (MQ=255)
                      tCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACAtcttct                                                                                                                                <  2:5497440/150‑1 (MQ=255)
                       cGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTa                                                                                                                               <  2:6371579/150‑1 (MQ=255)
                               gTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGcgcg                                                                                                                       >  1:6966511/1‑150 (MQ=255)
                                  aCATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACt                                                                                                                    <  2:1933647/150‑1 (MQ=255)
                                   cATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACtt                                                                                                                   >  1:8363907/1‑150 (MQ=255)
                                    aTCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCTTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTc                                                                                                                  <  2:8455647/150‑1 (MQ=255)
                                      cACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAgcgc                                                                                                                        <  1:7027778/142‑1 (MQ=255)
                                       aCCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAg                                                                                                               <  2:7141013/150‑1 (MQ=255)
                                        ccGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAgg                                                                                                              <  1:258682/150‑1 (MQ=255)
                                         cGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGc                                                                                                             <  2:7382187/150‑1 (MQ=255)
                                         cGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGc                                                                                                             >  2:510425/1‑150 (MQ=255)
                                         cGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGc                                                                                                             >  1:291953/1‑150 (MQ=255)
                                                cTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCATCACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGc                                                                                                      >  2:390780/1‑150 (MQ=255)
                                                 tCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTATACAAGCGCGACTTCTAGGCCCGCAGCg                                                                                                     >  2:1375061/1‑150 (MQ=255)
                                                  cAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGt                                                                                                    >  1:5027461/1‑150 (MQ=255)
                                                   aGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTg                                                                                                   <  1:1356839/150‑1 (MQ=255)
                                                     ccTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGTCCCGCAGCGTggc                                                                                                 >  2:3925519/1‑150 (MQ=255)
                                                     ccTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTggc                                                                                                 >  2:8565453/1‑150 (MQ=255)
                                                     ccTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTggc                                                                                                 >  2:3317086/1‑150 (MQ=255)
                                                      cTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTggcg                                                                                                <  1:421745/150‑1 (MQ=255)
                                                       ttCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTggcgg                                                                                               >  1:3425448/1‑150 (MQ=255)
                                                        tCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTggcggc                                                                                              <  1:3754754/150‑1 (MQ=255)
                                                        tCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCATTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTggcggc                                                                                              <  2:2257133/150‑1 (MQ=255)
                                                          gTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCa                                                                                            <  2:38208/150‑1 (MQ=255)
                                                           ttACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTAGCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCAt                                                                                           <  1:2041215/150‑1 (MQ=255)
                                                           ttACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCAt                                                                                           >  1:7616902/1‑150 (MQ=255)
                                                              cGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCaa                                                                                        <  2:7026257/150‑1 (MQ=255)
                                                               ggggTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAac                                                                                       <  2:5167317/150‑1 (MQ=255)
                                                                 ggTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGccc                                                                                                           <  2:2095488/128‑1 (MQ=255)
                                                                    gggCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAacggacg                                                                                  <  2:3612069/150‑1 (MQ=255)
                                                                    gggCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAacggacg                                                                                  <  1:1910671/150‑1 (MQ=255)
                                                                         tgtTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGgc                                                                             >  2:309489/1‑150 (MQ=255)
                                                                              gAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCCTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCt                                                                        >  1:3091688/1‑150 (MQ=255)
                                                                               aaCCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTc                                                                       <  2:291953/150‑1 (MQ=255)
                                                                                 ccGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGa                                                                     <  1:1009027/150‑1 (MQ=255)
                                                                                 ccGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGa                                                                     <  2:5045569/150‑1 (MQ=255)
                                                                                     ccAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGaa                                                                 <  2:6373297/150‑1 (MQ=255)
                                                                                     ccAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGaa                                                                 <  1:5222133/150‑1 (MQ=255)
                                                                                            gATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTg                                                          >  1:2627736/1‑150 (MQ=255)
                                                                                             aTCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTgg                                                         >  2:6056300/1‑150 (MQ=255)
                                                                                                  gttgcgttACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCg                                                    >  2:3559905/1‑150 (MQ=255)
                                                                                                   ttgcgttACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCgg                                                   <  1:6964835/150‑1 (MQ=255)
                                                                                                   ttgcgttACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCgg                                                   <  1:1063767/150‑1 (MQ=255)
                                                                                                   ttgcgttACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCgg                                                   <  1:8106012/150‑1 (MQ=255)
                                                                                                       gttACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGa                                               <  1:1103661/150‑1 (MQ=255)
                                                                                                        ttACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAg                                              >  2:4843560/1‑150 (MQ=255)
                                                                                                        ttACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGACTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAg                                              >  2:3883724/1‑150 (MQ=255)
                                                                                                          aCAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAggg                                            <  2:6700218/150‑1 (MQ=255)
                                                                                                            aaGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCg                                          <  1:2467097/150‑1 (MQ=255)
                                                                                                              gCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCggt                                        >  2:4387820/1‑150 (MQ=255)
                                                                                                              gCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCggt                                        >  1:7058020/1‑150 (MQ=255)
                                                                                                               cgcagcaagcaGCTCCCCCTCGAGGCCGCCAGCGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGGGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCggtg                                       <  2:3425448/147‑1 (MQ=255)
                                                                                                                tGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCggtgg                                      >  2:5463053/1‑150 (MQ=255)
                                                                                                                  aGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCggtgggg                                    >  2:5573117/1‑150 (MQ=255)
                                                                                                                   gCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCggtggggt                                   >  1:5266681/1‑150 (MQ=255)
                                                                                                                   gCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCggtggggt                                   >  1:5494442/1‑150 (MQ=255)
                                                                                                                    cAAGAAGGTCTCCCTCGAGGCCGCCAGGGGGCGGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCACCGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCggtggggtg                                  <  1:6187021/150‑1 (MQ=255)
                                                                                                                      agcagGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCACCGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGggtggt                                <  2:516358/147‑1 (MQ=255)
                                                                                                                       gaagGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGgtggtg                               <  1:1248725/150‑1 (MQ=255)
                                                                                                                       gaagGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGgtggtg                               <  1:2653488/150‑1 (MQ=255)
                                                                                                                         agGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGgc                             <  2:1813735/150‑1 (MQ=255)
                                                                                                                           gTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGgcgc                           >  1:2050995/1‑150 (MQ=255)
                                                                                                                            tCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGcgcg                          >  1:3188300/1‑150 (MQ=255)
                                                                                                                             cTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGt                         >  1:8442386/1‑150 (MQ=255)
                                                                                                                                ccTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTAcc                      >  2:6824839/1‑150 (MQ=255)
                                                                                                                                 cTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCg                     <  2:2313767/150‑1 (MQ=255)
                                                                                                                                 cTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCg                     <  2:2190573/150‑1 (MQ=255)
                                                                                                                                  tCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCgg                    >  1:6378995/1‑150 (MQ=255)
                                                                                                                                  gcaaGGCCGCCCGTGGGCTGGCAAGCTCACTGGACATCGTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGGGGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCgg                    >  2:4455417/4‑150 (MQ=255)
                                                                                                                                  gcaaGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGGTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCgg                    >  2:1203978/4‑150 (MQ=255)
                                                                                                                                         cgccAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGGGACGATc             <  2:523114/150‑1 (MQ=255)
                                                                                                                                          tccAGTGAGCTTGCCAGCCCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGTACTCGTTGGGGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGGGACGATcg            >  2:351093/2‑149 (MQ=255)
                                                                                                                                          tccAGTGAGCTTGCCAGCCCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGGGACGATcc            >  2:783992/2‑149 (MQ=255)
                                                                                                                                          gccAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGGGACGATCa            >  2:2752781/1‑150 (MQ=255)
                                                                                                                                          gccAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGGGACGATCa            >  2:6593784/1‑150 (MQ=255)
                                                                                                                                           ccAGTGAGCTTGCCAGCCCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGGGACGATCAg           >  2:2581484/1‑150 (MQ=255)
                                                                                                                                            cAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGGGACGATCAgg          <  1:6769815/150‑1 (MQ=255)
                                                                                                                                              gTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGGGACGATCAGGtt        <  2:2703979/150‑1 (MQ=255)
                                                                                                                                               tGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGGGACGATCAGGTTg       <  2:456591/150‑1 (MQ=255)
                                                                                                                                               tGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGGGACGATCAGGTTg       <  1:4512578/150‑1 (MQ=255)
                                                                                                                                                 ggCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTCGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGGGACGATCAGGTTGgt     <  1:94686/150‑1 (MQ=255)
                                                                                                                                                 ggCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGGGACGATCAGGTTGgt     <  1:5156614/150‑1 (MQ=255)
                                                                                                                                                 ggCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGGGACGATCAGGTTGgt     <  2:1605978/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TCTGGCAACAGCACCAGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGGGACGATCAGGTTGGTGTA  >  NC_002947/3296168‑3296465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑