BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,296,320	A→C	5.1%	A1213A (GCA→GCC)	PP_2893 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,296,320	0	A	C	5.1%	736.9 / 20.4	215	A1213A (GCA→GCC)	PP_2893	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (85/119); new base C (5/6); total (90/125)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01

Only 100 of 222 total aligned reads displayed.

GCAACAGCACCAGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGGGACGATCAGGTTGGTGTATTC  >  NC_002947/3296172‑3296468

gCAACAGCACCAGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCaa                                                                                                                                                     >  1:4693004/1‑150 (MQ=255)

gCAACAGCACCAGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCaa                                                                                                                                                     >  2:7816814/1‑150 (MQ=255)

 cAACAGCACCAGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAg                                                                                                                                                    <  2:920037/150‑1 (MQ=255)

  aaCAGCACCAGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGc                                                                                                                                                   <  1:5954400/150‑1 (MQ=255)

      gCACCAGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGAGCTTGCCAGCCCAc                                                                                                                                               <  1:2045295/150‑1 (MQ=255)

       cACCAGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACt                                                                                                                                              <  2:8856346/150‑1 (MQ=255)

        aCCAGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTg                                                                                                                                             <  1:8079812/150‑1 (MQ=255)

        aCCAGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAACGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTg                                                                                                                                             <  1:2673913/150‑1 (MQ=255)

         ccAGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTgg                                                                                                                                            >  1:2545239/1‑150 (MQ=255)

          cAGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGAGCTTGCCAGCCCACTggc                                                                                                                                           <  1:9054823/150‑2 (MQ=255)

           aGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGAc                                                                                                                                          >  2:94686/1‑150 (MQ=255)

           aGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGAGCTTGCCAGCCCACTggcg                                                                                                                                          <  2:7263130/150‑3 (MQ=255)

                ggTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACAtctt                                                                                                                                     <  1:972460/150‑1 (MQ=255)

                ggTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACAtctt                                                                                                                                     <  2:3676540/150‑1 (MQ=255)

                  tCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACAtcttct                                                                                                                                   <  2:5497538/150‑1 (MQ=255)

                   cGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTa                                                                                                                                  <  1:8281145/150‑1 (MQ=255)

                          ggTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAgcgc                                                                                                                           <  2:7478782/150‑1 (MQ=255)

                           gTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGcgcg                                                                                                                          >  1:6966511/1‑150 (MQ=255)

                              aCATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACt                                                                                                                       <  2:1933642/150‑1 (MQ=255)

                                aTCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCTTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTc                                                                                                                     <  2:8455647/150‑1 (MQ=255)

                                aTCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTc                                                                                                                     >  2:6647974/1‑150 (MQ=255)

                                 tCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCt                                                                                                                    >  1:7141013/1‑150 (MQ=255)

                                  cACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAgcgc                                                                                                                           >  2:7027778/1‑142 (MQ=255)

                                            cTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCATCACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGc                                                                                                         >  2:390808/1‑150 (MQ=255)

                                             tCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGcctcgaatg                                                                                                        >  2:4526537/1‑143 (MQ=255)

                                               aGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTg                                                                                                      <  1:1356839/150‑1 (MQ=255)

                                                 ccTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTggc                                                                                                    >  2:8565453/1‑150 (MQ=255)

                                                  cTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTggcg                                                                                                   <  1:421745/150‑1 (MQ=255)

                                                   ttCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTggcgg                                                                                                  >  1:3425448/1‑150 (MQ=255)

                                                   ttCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTggcgg                                                                                                  <  2:1701618/150‑1 (MQ=255)

                                                    tCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTggcggc                                                                                                 <  1:3754754/150‑1 (MQ=255)

                                                    tCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTggcggc                                                                                                 <  1:8399702/150‑1 (MQ=255)

                                                      gTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCa                                                                                               <  1:7545158/150‑1 (MQ=255)

                                                       ttACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCAt                                                                                              >  1:7616902/1‑150 (MQ=255)

                                                       ttACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCAt                                                                                              <  2:7552387/150‑1 (MQ=255)

                                                            gggTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAacg                                                                                         >  2:6347224/1‑150 (MQ=255)

                                                             ggTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGccc                                                                                                              <  2:2095488/128‑1 (MQ=255)

                                                             ggTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGccc                                                                                                              >  1:2095488/1‑128 (MQ=255)

                                                               tGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCGGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAacggac                                                                                      <  1:2638757/150‑1 (MQ=255)

                                                                gggCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAacggacg                                                                                     <  1:1910671/150‑1 (MQ=255)

                                                                    gtgtTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTgg                                                                                 >  1:7041116/1‑150 (MQ=255)

                                                                     tgtTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGgc                                                                                >  2:309489/1‑150 (MQ=255)

                                                                      gtTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGgcc                                                                               <  2:3862230/150‑1 (MQ=255)

                                                                           aaCCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTc                                                                          <  2:291953/150‑1 (MQ=255)

                                                                             ccGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGa                                                                        <  2:5045171/150‑1 (MQ=255)

                                                                             ccGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGa                                                                        <  1:1009027/150‑1 (MQ=255)

                                                                                 ccAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGaa                                                                    <  1:5222133/150‑1 (MQ=255)

                                                                                 ccAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACAACTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGaa                                                                    <  2:6373027/150‑1 (MQ=255)

                                                                                  cAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAAc                                                                   <  1:8991825/150‑1 (MQ=255)

                                                                                       cGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTTGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGtt                                                              <  2:5927555/150‑1 (MQ=255)

                                                                                        gATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTg                                                             >  1:2627636/1‑150 (MQ=255)

                                                                                             cgttgcgttACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTc                                                        <  2:6213986/150‑1 (MQ=255)

                                                                                              gttgcgttACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTATGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCg                                                       >  1:2955730/1‑150 (MQ=255)

                                                                                              gttgcgttACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCg                                                       <  2:5266317/150‑1 (MQ=255)

                                                                                              gttgcgttACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCg                                                       >  2:3560943/1‑150 (MQ=255)

                                                                                               ttgcgttACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCgg                                                      <  2:5464335/150‑1 (MQ=255)

                                                                                               ttgcgttACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCgg                                                      <  1:1063767/150‑1 (MQ=255)

                                                                                                   gttACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGa                                                  <  1:1103661/150‑1 (MQ=255)

                                                                                                      aCAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAggg                                               >  2:158541/1‑150 (MQ=255)

                                                                                                        aaGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCg                                             <  1:2467097/150‑1 (MQ=255)

                                                                                                          gCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCggt                                           <  2:4451036/150‑1 (MQ=255)

                                                                                                           cgcagcaagcaGCTCCCCCTCGAGGCCGCCAGCGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGGGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCggtg                                          <  2:3425448/147‑1 (MQ=255)

                                                                                                               gCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCggtggggt                                      <  2:2259388/150‑1 (MQ=255)

                                                                                                               gCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCggtggggt                                      >  1:5266681/1‑150 (MQ=255)

                                                                                                               gCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCggtggggt                                      >  1:5494442/1‑150 (MQ=255)

                                                                                                                cAAGAAGGTCTCCCTCGAGGCCGCCAGGGGGCGGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCACCGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCggtggggtg                                     <  1:6187021/150‑1 (MQ=255)

                                                                                                                 aagaagGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCggtggggtgg                                    >  1:6886931/1‑150 (MQ=255)

                                                                                                                  cgacggTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACAGCTTCTACACGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGggtggt                                   <  2:516368/146‑1 (MQ=255)

                                                                                                                  agaagGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACACGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGggtggt                                   <  2:6839192/150‑1 (MQ=255)

                                                                                                                  agaagGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGggtggt                                   <  2:6781347/150‑1 (MQ=255)

                                                                                                                  agaagGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGggtggt                                   <  1:3814295/150‑1 (MQ=255)

                                                                                                                   gaagGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGgtggtg                                  <  1:1248725/150‑1 (MQ=255)

                                                                                                                   gaagGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGgtggtg                                  <  2:5167851/150‑1 (MQ=255)

                                                                                                                     agGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGgc                                <  2:1813735/150‑1 (MQ=255)

                                                                                                                        tCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGcgcg                             >  2:3934016/1‑150 (MQ=255)

                                                                                                                         cTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGt                            >  1:8442386/1‑150 (MQ=255)

                                                                                                                           aCCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTAc                          >  2:3889547/1‑150 (MQ=255)

                                                                                                                            ccTCGAGGCCGCCAGTGGGTTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTAcc                         <  1:2791519/150‑1 (MQ=255)

                                                                                                                             cTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCg                        >  2:4223582/1‑150 (MQ=255)

                                                                                                                             cTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCg                        <  2:2190573/150‑1 (MQ=255)

                                                                                                                             cTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCg                        <  2:2313781/150‑1 (MQ=255)

                                                                                                                              tCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCgg                       >  1:6378995/1‑150 (MQ=255)

                                                                                                                              tCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCgg                       >  2:3297463/1‑150 (MQ=255)

                                                                                                                              tCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCgg                       >  1:4952377/1‑150 (MQ=255)

                                                                                                                              gcaaGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGGTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCgg                       >  2:1203978/4‑150 (MQ=255)

                                                                                                                                gAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGGGa                     <  2:4693004/150‑1 (MQ=255)

                                                                                                                                gAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGGGa                     >  2:6490620/1‑150 (MQ=255)

                                                                                                                                 aGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCgggta                    <  1:3106468/150‑3 (MQ=255)

                                                                                                                                     cgccAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGGGACGATc                <  2:5095677/150‑1 (MQ=255)

                                                                                                                                     cgccAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGGGACGATc                >  2:5120888/1‑150 (MQ=255)

                                                                                                                                      tccAGTGAGCTTGCCAGCCCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGTACTCGTTGGGGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGGGACGATcg               >  2:351093/2‑149 (MQ=255)

                                                                                                                                      tccAGTGAGCTTGCCAGCCCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGGGACGATcc               >  2:783992/2‑149 (MQ=255)

                                                                                                                                      gccAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGGGACGATCa               >  2:2752781/1‑150 (MQ=255)

                                                                                                                                      gccAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGAGACGATCa               >  2:3550304/1‑150 (MQ=255)

                                                                                                                                        cAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGGGACGATCAgg             <  1:6769815/150‑1 (MQ=255)

                                                                                                                                          gTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGGGACGATCAGGtt           <  2:2703979/150‑1 (MQ=255)

                                                                                                                                           tGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGGGACGATCAGGTTg          <  1:4512578/150‑1 (MQ=255)

                                                                                                                                             ggctggctAGCTGTCTGGAGATCTTCTACAAGCGCGTCTTCTAGGCCCGCAGCGTGGCGGCAATCTACGGACGGTGGCCGCCTCGATGATGTCGTTGGTGTCGGTTGAGGGCGGTGGGGTGGTGGAGGGTACCGGGACGATCtggttggt        <  1:9155175/150‑1 (MQ=255)

                                                                                                                                             ggCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGGGACGATCAGGTTGgt        <  1:5156614/150‑1 (MQ=255)

                                                                                                                                                  gCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGGGACGATCAGGTTGGTGTAtt   >  2:2253065/1‑150 (MQ=255)

GCAACAGCACCAGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGGGACGATCAGGTTGGTGTATTC  >  NC_002947/3296172‑3296468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`