Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,296,324 T→C 5.0% S1215P (TCA→CCA)  PP_2893 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,296,3240TC 5.0% 715.5 / 15.3 218S1215P (TCA→CCA) PP_2893hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (85/122);  new base C (5/6);  total (90/128)
Fisher's exact test for biased strand distribution p-value = 7.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

Only 100 of 222 total aligned reads displayed.

AGCACCAGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGGGACGATCAGGTTGGTGTATTCCTCGA  >  NC_002947/3296177‑3296473
                                                                                                                                                   |                                                                                                                                                     
 gCACCAGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCAc                                                                                                                                                    >  1:9110990/1‑150 (MQ=255)
  cACCAGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACt                                                                                                                                                   <  2:8856346/150‑1 (MQ=255)
   aCCAGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTg                                                                                                                                                  <  1:41320/150‑1 (MQ=255)
   aCCAGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTg                                                                                                                                                  <  1:2674014/150‑1 (MQ=255)
    cccgCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGTGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTgg                                                                                                                                                 <  2:7505992/147‑1 (MQ=255)
     cAGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGAGCTTGCCAGCCCACTggc                                                                                                                                                <  1:9054521/150‑2 (MQ=255)
      aGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGAc                                                                                                                                               >  2:94686/1‑150 (MQ=255)
      aGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGAGCTTGCCAGCCCACTggcg                                                                                                                                               <  2:7263130/150‑3 (MQ=255)
           ggTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACAtctt                                                                                                                                          <  2:3676540/150‑1 (MQ=255)
             tCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACAtcttct                                                                                                                                        <  2:5497538/150‑1 (MQ=255)
                ccAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACa                                                                                                                                     >  2:1312067/1‑150 (MQ=255)
                      gTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGcgcg                                                                                                                               >  1:6966511/1‑150 (MQ=255)
                         aCATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACt                                                                                                                            <  2:1933642/150‑1 (MQ=255)
                          cATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACtt                                                                                                                           >  1:8363907/1‑150 (MQ=255)
                           aTCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTc                                                                                                                          >  2:6647974/1‑150 (MQ=255)
                            tCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCt                                                                                                                         >  1:7141013/1‑150 (MQ=255)
                              aCCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAg                                                                                                                       >  2:8486103/1‑150 (MQ=255)
                               ccGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAgg                                                                                                                      <  1:258682/150‑1 (MQ=255)
                                cGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGc                                                                                                                     <  2:7382187/150‑1 (MQ=255)
                                cGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGc                                                                                                                     >  1:291953/1‑150 (MQ=255)
                                       cTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCATCACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGc                                                                                                              >  2:390808/1‑150 (MQ=255)
                                        tCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGcctcgaatg                                                                                                             >  2:4526537/1‑143 (MQ=255)
                                         cAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGt                                                                                                            >  2:2705339/1‑150 (MQ=255)
                                          aGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTg                                                                                                           <  1:1356839/150‑1 (MQ=255)
                                            ccTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTggc                                                                                                         >  2:8565453/1‑150 (MQ=255)
                                             cTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTggcg                                                                                                        <  1:421886/150‑1 (MQ=255)
                                              ttCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTggcgg                                                                                                       <  2:1701618/150‑1 (MQ=255)
                                                  ttACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTAGCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCAt                                                                                                   <  1:2041215/150‑1 (MQ=255)
                                                     cGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCaa                                                                                                <  2:7026257/150‑1 (MQ=255)
                                                       gggTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAacg                                                                                              >  2:6347224/1‑150 (MQ=255)
                                                        ggTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGccc                                                                                                                   <  2:2095488/128‑1 (MQ=255)
                                                               gtgtTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTgg                                                                                      >  1:7041116/1‑150 (MQ=255)
                                                                tgtTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGgc                                                                                     >  2:309489/1‑150 (MQ=255)
                                                                 gtTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGgcc                                                                                    <  1:7555023/150‑1 (MQ=255)
                                                                 gtTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGgcc                                                                                    <  2:3862230/150‑1 (MQ=255)
                                                                     gAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCCTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCt                                                                                >  1:3091688/1‑150 (MQ=255)
                                                                      aaCCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTc                                                                               <  2:291953/150‑1 (MQ=255)
                                                                        ccGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGa                                                                             <  1:1009027/150‑1 (MQ=255)
                                                                        ccGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGa                                                                             <  2:1107066/150‑1 (MQ=255)
                                                                        ccGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGa                                                                             <  2:5045171/150‑1 (MQ=255)
                                                                           gCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGa                                                                          >  1:3646614/1‑150 (MQ=255)
                                                                            ccAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGaa                                                                         <  1:5222133/150‑1 (MQ=255)
                                                                            ccAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACAACTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGaa                                                                         <  2:6373027/150‑1 (MQ=255)
                                                                               acacGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTc                                                                      >  1:7761860/1‑150 (MQ=255)
                                                                                 acGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGt                                                                    <  1:3780679/150‑1 (MQ=255)
                                                                                  cGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTTGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGtt                                                                   <  2:5927555/150‑1 (MQ=255)
                                                                                   gATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTg                                                                  <  1:169013/150‑1 (MQ=255)
                                                                                   gATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTg                                                                  >  1:2627736/1‑150 (MQ=255)
                                                                                       gcgttgcgttACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGgtgt                                                              >  1:5759184/1‑150 (MQ=255)
                                                                                        cgttgcgttACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTc                                                             <  2:6213986/150‑1 (MQ=255)
                                                                                         gttgcgttACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCg                                                            <  2:5266317/150‑1 (MQ=255)
                                                                                         gttgcgttACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCg                                                            >  2:3560943/1‑150 (MQ=255)
                                                                                          ttgcgttACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCgg                                                           <  2:5464335/150‑1 (MQ=255)
                                                                                          ttgcgttACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCgg                                                           <  1:1063767/150‑1 (MQ=255)
                                                                                          ttgcgttACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCgg                                                           <  1:8106012/150‑1 (MQ=255)
                                                                                              gttACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGa                                                       <  1:1103661/150‑1 (MQ=255)
                                                                                               ttACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAg                                                      >  1:8437611/1‑150 (MQ=255)
                                                                                               ttACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAg                                                      >  1:6090624/1‑150 (MQ=255)
                                                                                                 aCAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAggg                                                    >  2:158541/1‑150 (MQ=255)
                                                                                                   aaGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCg                                                  <  1:2467097/150‑1 (MQ=255)
                                                                                                     gCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCggt                                                <  2:4451036/150‑1 (MQ=255)
                                                                                                      cgcagcaagcaGCTCCCCCTCGAGGCCGCCAGCGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGGGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCggtg                                               <  2:3425448/147‑1 (MQ=255)
                                                                                                          gCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCggtggggt                                           <  2:2259388/150‑1 (MQ=255)
                                                                                                           cAAGAAGGTCTCCCTCGAGGCCGCCAGGGGGCGGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCACCGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCggtggggtg                                          <  1:6187021/150‑1 (MQ=255)
                                                                                                            aagaagGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCggtggggtgg                                         >  1:6886931/1‑150 (MQ=255)
                                                                                                             cgacggTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACAGCTTCTACACGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGggtggt                                        <  2:516368/146‑1 (MQ=255)
                                                                                                              gaagGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGgtggtg                                       <  2:5167851/150‑1 (MQ=255)
                                                                                                              gaagGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGgtggtg                                       <  1:1248725/150‑1 (MQ=255)
                                                                                                              gaagGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGgtggtg                                       <  1:2653488/150‑1 (MQ=255)
                                                                                                                agGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGgc                                     <  2:1813735/150‑1 (MQ=255)
                                                                                                                  gTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGgcgc                                   >  1:2050995/1‑150 (MQ=255)
                                                                                                                   tCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGcgcg                                  >  2:3934016/1‑150 (MQ=255)
                                                                                                                   tCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGcgcg                                  >  1:3188300/1‑150 (MQ=255)
                                                                                                                      aCCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTAc                               >  2:3889547/1‑150 (MQ=255)
                                                                                                                       ccTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTAcc                              >  2:6824839/1‑150 (MQ=255)
                                                                                                                       ccTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTAcc                              <  1:8455589/150‑1 (MQ=255)
                                                                                                                        cTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCg                             >  2:4223582/1‑150 (MQ=255)
                                                                                                                        cTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCg                             <  2:2313781/150‑1 (MQ=255)
                                                                                                                        cTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCg                             <  2:2190573/150‑1 (MQ=255)
                                                                                                                         tCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCgg                            >  1:4952377/1‑150 (MQ=255)
                                                                                                                         tCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCgg                            >  2:3297463/1‑150 (MQ=255)
                                                                                                                         tCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCgg                            >  1:6378995/1‑150 (MQ=255)
                                                                                                                           gAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGGGa                          <  2:4693004/150‑1 (MQ=255)
                                                                                                                           gAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGGGa                          >  2:6490620/1‑150 (MQ=255)
                                                                                                                                cgccAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGGGACGATc                     <  2:5095677/150‑1 (MQ=255)
                                                                                                                                cgccAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGGGACGATc                     >  2:5120888/1‑150 (MQ=255)
                                                                                                                                 tccAGTGAGCTTGCCAGCCCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGTACTCGTTGGGGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGGGACGATcg                    >  2:351093/2‑149 (MQ=255)
                                                                                                                                 tccAGTGAGCTTGCCAGCCCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGGGACGATcc                    >  2:783992/2‑149 (MQ=255)
                                                                                                                                 gccAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGAGACGATCa                    >  2:3550304/1‑150 (MQ=255)
                                                                                                                                  ccAGTGAGCTTGCCAGCCCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGGGACGATCAg                   >  2:2814530/1‑150 (MQ=255)
                                                                                                                                   cAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGGGACGATCAgg                  <  1:6769815/150‑1 (MQ=255)
                                                                                                                                      tGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGGGACGATCAGGTTg               <  1:4512578/150‑1 (MQ=255)
                                                                                                                                        ggCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTCGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGGGACGATCAGGTTGgt             <  1:94686/150‑1 (MQ=255)
                                                                                                                                        ggCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGGGACGATCAGGTTGgt             <  1:5156614/150‑1 (MQ=255)
                                                                                                                                           tGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGGGACGATCAGGTTGGTGTa          <  1:5474345/150‑1 (MQ=255)
                                                                                                                                             gCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGGGACGATCAGGTTGGTGTAtt        >  2:7803244/1‑150 (MQ=255)
                                                                                                                                             gCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGGGACGATCAGGTTGGTGTAtt        >  2:2253065/1‑150 (MQ=255)
                                                                                                                                                 gCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGGGACGATCAGGTTGGTGTATTCCTc    <  2:6966511/150‑1 (MQ=255)
                                                                                                                                                 gCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGGGACGATCAGGTTGGTGTATTCCTc    <  1:2353898/150‑1 (MQ=255)
                                                                                                                                                   tCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGGGACGATCAGGTTGGTGTATTCCTCGa  <  2:6779136/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
AGCACCAGCGTGGTCGCCAGCGGTAACATCACCGAGAAGCTCAGCCTTCGTTACGGGGTGGGCGTGTTCGAACCGGCCAACACGATCGCGTTGCGTTACAAGCTGAGCAAGAAGGTCTACCTCGAGGCCGCCAGTGGGCTGGCAAGCTCACTGGACATCTTCTACAAGCGCGACTTCTAGGCCCGCAGCGTGGCGGCCATCAACGGACGGTGGCCGCCTCGATGAACTCGTTGGTGTCGGGTGAGGGCGGTGGGGTGGTGGCGCGTACCGGGACGATCAGGTTGGTGTATTCCTCGA  >  NC_002947/3296177‑3296473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑