BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	14,895	G→A	5.3%	E522E (GAG→GAA)	gyrB →	DNA gyrase subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	14,895	0	G	A	5.3%	904.4 / 23.4	282	E522E (GAG→GAA)	gyrB	DNA gyrase subunit B
Reads supporting (aligned to +/- strand): ref base G (139/128); new base A (8/7); total (147/135)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 282 total aligned reads displayed.

CACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGCCTGCACCTGGACG  >  NC_002947/14748‑15043

cACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACCTCGACAAACTGCGTGATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCGGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCt                                                                                                                                                    <  2:7849722/150‑1 (MQ=255)

  cTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTgg                                                                                                                                                  >  1:4210648/1‑150 (MQ=255)

  cTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTgg                                                                                                                                                  >  2:5313370/1‑150 (MQ=255)

  cTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGATgg                                                                                                                                                  <  2:4251337/150‑1 (MQ=255)

     cgcTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTc                                                                                                                                                >  1:5855778/1‑149 (MQ=255)

        tGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGc                                                                                                                                            <  1:4495833/150‑1 (MQ=255)

          ggCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGt                                                                                                                                          >  2:1788058/1‑150 (MQ=255)

           gCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTg                                                                                                                                         <  1:1656926/150‑1 (MQ=255)

           gCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTg                                                                                                                                         >  2:1447800/1‑150 (MQ=255)

            cTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTgg                                                                                                                                        <  2:4224826/150‑1 (MQ=255)

             tgtgGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTCCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGc                                                                                                                                       >  2:1105777/1‑150 (MQ=255)

                ggCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTAc                                                                                                                                    <  1:597973/150‑1 (MQ=255)

                ggCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTAc                                                                                                                                    <  2:1181980/150‑1 (MQ=255)

                ggCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTAc                                                                                                                                    <  2:1923065/150‑1 (MQ=255)

                      ggCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCtat                                                                                                                              <  1:692894/150‑1 (MQ=255)

                          gcgAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTg                                                                                                                          <  2:2447884/150‑1 (MQ=255)

                              agagTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCa                                                                                                                      <  2:6335653/150‑1 (MQ=255)

                                      acaTCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCcgccgc                                                                                                              <  1:7554178/150‑1 (MQ=255)

                                       tcctgACAAGCTGCGCTACCATAAGATCATCATCATGACGGATGCTGACGTCGACGGTGCGCATATTCGTACGCTGCTGTTGACCTTCTTCTTCCGTCAGATGCCGGAACTGATCGAGCGTGGCCATATCTATATCGCGCAGCCGCCGCt                                                                                                             >  2:4986176/5‑150 (MQ=255)

                                       tcctgACAAGCTGCGCTACCATAAGATCATCATCATGACGGATGCTGACGTCGACGGTGCGCATATTCGTACGCTGCTGTTGACCTTCTTCTTCCGTCAGATGCCGGAACTGATCGAGCGTGGCCATATCTATATCGCGCAGCCGCCGCt                                                                                                             >  1:2850627/5‑150 (MQ=255)

                                        aTCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTg                                                                                                            >  1:7303616/1‑150 (MQ=255)

                                        aTCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTg                                                                                                            >  2:3142616/1‑150 (MQ=255)

                                           gACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTAc                                                                                                         <  1:2856350/150‑1 (MQ=255)

                                             cAAGCTGCGCTACCATAAGATCATCATCATGACGGATGCTGACGTCGACGGTGCGCATATTCGTACGCTGCTGTTGACCTTCTTCTTCCGTCAGATGCCGGAACTGATCGAGCGTGGCCATATCTATATCGCGCAGCCGCCGCTCTATaa                                                                                                       <  1:2656823/150‑1 (MQ=255)

                                             cAAGCTGCGCTACCATAAGATCATCATCATGACGGATGCTGACGTCGACGGTGCGCATATTCGTACGCTGCTGTTGACCTTCTTCTTCCGTCAGATGCCGGAACTGATCGAGCGTGGCCATATCTATATCGCGCAGCCGCCGCTCTATaa                                                                                                       <  2:7299176/150‑1 (MQ=255)

                                             cAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACaa                                                                                                       <  1:5018793/150‑1 (MQ=255)

                                             cAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACaa                                                                                                       <  1:144965/150‑1 (MQ=255)

                                               aaCTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAgg                                                                                                     <  2:4048133/150‑1 (MQ=255)

                                                gcTGCGCTACCATAAGATCATCATCATGACGGATGCTGACGTCGACGGTGCGCATATTCGTACGCTGCTGTTGACCTTCTTCTTCCGTCAGATGCCGGAACTGATCGAGCGTGGCCATATCTATATCGCGCAGCCGCCGCTCTATAAGGt                                                                                                    >  2:5704951/2‑150 (MQ=255)

                                                  tGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGa                                                                                                  >  2:7213606/1‑150 (MQ=255)

                                                   gcgcTACCATAAGATCATCATCATGACGGATGCTGACGTCGACGGTGCGCATATTCGTACGCTGCTGTTGACCTTCTTCTTCCGTCAGATGCCGGAACTGATCGAGCGTGGCCATATCTATATCGCGCAGCCGCCGCTCTATAAGGTgtc                                                                                                 >  2:3890478/1‑148 (MQ=255)

                                                     gatATCACAACATCAAAATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCAGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAAc                                                                                               >  2:2260637/3‑150 (MQ=255)

                                                     gTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAAc                                                                                               >  1:6389589/1‑150 (MQ=255)

                                                     gTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAAc                                                                                               <  2:5566960/150‑1 (MQ=255)

                                                       tATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGccgccg                                                                                                               >  2:3697209/1‑132 (MQ=255)

                                                       tATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGa                                                                                             >  2:5928160/1‑150 (MQ=255)

                                                          cacaACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGc                                                                                          <  1:1265703/150‑1 (MQ=255)

                                                           acaacaTCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGTGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACCAGGTGAAACGAGGCa                                                                                         >  2:8302251/1‑150 (MQ=255)

                                                           acaacaTCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCa                                                                                         >  2:1577939/1‑150 (MQ=255)

                                                           acaacaTCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCa                                                                                         >  2:1395459/1‑150 (MQ=255)

                                                            caacaTCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGAGCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCaa                                                                                        >  1:3800824/1‑150 (MQ=255)

                                                            caacaTCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCaa                                                                                        <  2:8614852/150‑1 (MQ=255)

                                                             aacaTCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAg                                                                                       >  1:7373952/1‑150 (MQ=255)

                                                             aacaTCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAg                                                                                       >  1:8636946/1‑150 (MQ=255)

                                                                  catcatcatGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGa                                                                                  <  1:4304034/150‑1 (MQ=255)

                                                                    tcatcatGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGc                                                                                >  2:3948284/1‑150 (MQ=255)

                                                                    tcatcatGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGc                                                                                >  2:6253586/1‑150 (MQ=255)

                                                                     catcatGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCa                                                                               <  1:6200877/150‑1 (MQ=255)

                                                                       tcatGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGt                                                                             >  1:3615133/1‑150 (MQ=255)

                                                                            aCCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATc                                                                        >  2:5542899/1‑150 (MQ=255)

                                                                                  gCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGAc                                                                  <  2:8171438/150‑1 (MQ=255)

                                                                                    tGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGa                                                                >  1:8006767/1‑150 (MQ=255)

                                                                                    tGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGa                                                                >  1:5353113/1‑150 (MQ=255)

                                                                                    tGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGa                                                                >  1:2809359/1‑150 (MQ=255)

                                                                                     gACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGAt                                                               <  2:6455077/150‑1 (MQ=255)

                                                                                     gACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGAt                                                               >  2:3409119/1‑150 (MQ=255)

                                                                                      aCGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATg                                                              >  2:6901979/1‑150 (MQ=255)

                                                                                       cGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGa                                                             <  2:4348980/150‑1 (MQ=255)

                                                                                           gACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGcc                                                         >  1:3070832/1‑150 (MQ=255)

                                                                                              gggtCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATg                                                      <  2:2628063/147‑1 (MQ=255)

                                                                                                 tCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGaa                                                   <  1:5892875/150‑1 (MQ=255)

                                                                                                 tCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGaa                                                   <  1:549321/150‑1 (MQ=255)

                                                                                                   gCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAaga                                                 <  1:3279120/150‑1 (MQ=255)

                                                                                                   gCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAaga                                                 >  2:5372558/1‑150 (MQ=255)

                                                                                                    cacaTCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAagag                                                >  2:5857244/1‑150 (MQ=255)

                                                                                                       aTCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTAc                                             >  1:8159295/1‑150 (MQ=255)

                                                                                                        tCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACa                                            <  1:8680996/150‑1 (MQ=255)

                                                                                                        tCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACa                                            <  1:913972/150‑1 (MQ=255)

                                                                                                           gTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGa                                         <  1:8136991/150‑1 (MQ=255)

                                                                                                            tACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGAc                                        >  1:2159090/1‑150 (MQ=255)

                                                                                                               gctgctgctGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCa                                     >  2:8127783/1‑150 (MQ=255)

                                                                                                               gctgctgctGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCa                                     >  1:7939056/1‑150 (MQ=255)

                                                                                                                 tgctgctgACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGt                                   >  1:663900/1‑150 (MQ=255)

                                                                                                                 tgctgctgACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGt                                   <  1:4670773/150‑1 (MQ=255)

                                                                                                                  gctgctgACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTc                                  <  1:3148033/150‑1 (MQ=255)

                                                                                                                  gctgctgACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTc                                  <  2:2931466/150‑1 (MQ=255)

                                                                                                                   ctgctgACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCg                                 >  1:6093375/1‑150 (MQ=255)

                                                                                                                        gACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAaga                                                 >  2:4524665/1‑129 (MQ=255)

                                                                                                                        gACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGctct                            <  2:4098356/150‑1 (MQ=255)

                                                                                                                         aCCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTg                           <  1:5087762/150‑1 (MQ=255)

                                                                                                                         aCCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTg                           >  2:2822750/1‑150 (MQ=255)

                                                                                                                          ccTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTgg                          >  2:8817557/1‑150 (MQ=255)

                                                                                                                           cttcttcttCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGa                         >  2:7900507/1‑150 (MQ=255)

                                                                                                                           cttcttcttCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGa                         >  1:1001717/1‑150 (MQ=255)

                                                                                                                            ttcttcttcCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGaa                        >  2:5430664/1‑150 (MQ=255)

                                                                                                                            ttcttcttcCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGaa                        <  1:8995938/150‑1 (MQ=255)

                                                                                                                              ccagctcCGGCAGCTGACGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAaga                                                 <  2:6800148/118‑1 (MQ=255)

                                                                                                                              ccagctcCGGCAGCTGACGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAaga                                                 >  1:6800148/6‑123 (MQ=255)

                                                                                                                               ttcttcCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGAt                     <  1:1823353/150‑1 (MQ=255)

                                                                                                                               ttcttcCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGAt                     >  1:3983335/1‑150 (MQ=255)

                                                                                                                                  ttcCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGcc                  >  1:3386222/1‑150 (MQ=255)

                                                                                                                                      gTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGcc              <  2:8026198/150‑1 (MQ=255)

                                                                                                                                         aGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGCCTGc           >  1:4588927/1‑150 (MQ=255)

                                                                                                                                         aGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGCCTGc           >  1:7797572/1‑150 (MQ=255)

                                                                                                                                          gCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGCCTGCa          <  1:3704557/150‑1 (MQ=255)

                                                                                                                                           cTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGCCTGCAc         <  2:2035271/150‑1 (MQ=255)

                                                                                                                                            tGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGCCTGCAcc        >  2:8646903/1‑150 (MQ=255)

                                                                                                                                             gCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGCCTGCACCt       <  1:5313370/150‑1 (MQ=255)

                                                                                                                                              ccGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGCCTGCACCTg      <  2:1850596/150‑1 (MQ=255)

                                                                                                                                                ggAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGCCTGCACCTggt    <  1:1957563/150‑2 (MQ=255)

CACTGCGCTGGGCTGTGGCATCGGCCGCGAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGCCTGCACCTGGACG  >  NC_002947/14748‑15043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`