BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,356,995	C→T	5.9%	R61Q (CGG→CAG) ‡	PP_2950 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,356,995	0	C	T	5.9%	954.9 / 30.9	303	R61Q (CGG→CAG) ‡	PP_2950	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (153/131); new base T (15/3); total (169/134)
Fisher's exact test for biased strand distribution p-value = 1.49e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 319 total aligned reads displayed.

TCGTTGCCATAGGAGCGAAACCAGTTGCCCGAATCGTCGTGCCACTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGA  >  NC_002947/3356848‑3357144

tagTTTCCATAGTAGCGAAACAATTTGACCGAATCGTCGTGCCACTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCAACACCTCTTTTATCAACAGATAGTCCAGTTCTTTATTCCACTTGCGAGTTAGGAAGTCCCGa                                                                                                                                                     <  2:1714619/148‑1 (MQ=255)

tCGTTGCCATAGGAGCGAAACCAGTTGCCCGAATCGTCGTGCCACTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCTCGa                                                                                                                                                     <  2:8631640/150‑1 (MQ=255)

tCGTTGCCATAGGAGCGAAACCAGTTGCCCGAATCGTCGTGCCACTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGa                                                                                                                                                     <  2:2715153/150‑1 (MQ=255)

tCGTTGCCATAGGAGCGAAACCAGTTGCCCGAATCGTCGTGCCACTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGa                                                                                                                                                     <  1:181575/150‑1 (MQ=255)

tCGTTGCCATAGGAGCGAAACCAGTTGCCCGAATCGTCGTGCCACTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGa                                                                                                                                                     <  1:8391777/150‑1 (MQ=255)

tCGTTGCCATAGGAGCGAAACCAGTTGCCCGAATCGTCGTGCCACTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGa                                                                                                                                                     <  2:3752631/150‑1 (MQ=255)

     gCCATAGGAGCGAAACCAGTTGCCCGAATCGTCGTGCCACTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGcttc                                                                                                                                                <  1:7122260/150‑1 (MQ=255)

      ccATAGGAGCGAAACCAGTTGCCCGAATCGTCGTGCCACTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGcttct                                                                                                                                               >  1:2565744/1‑150 (MQ=255)

        aTAGGAGCGAAACCAGTTGCCCGAATCGTCGTGCCACTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCttcttc                                                                                                                                             >  2:8494567/1‑150 (MQ=255)

         tAGGAGCGAAACCAGTTGCCCGAATCGTCGTGCCACTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCa                                                                                                                                            <  2:2327238/150‑1 (MQ=255)

          aGGAGCGAAACCAGTTGCCCGAATCGTCGTGCCACTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCAc                                                                                                                                           <  1:4119812/150‑1 (MQ=255)

            gAGCGAAACCAGTTGCCCGAATCGTCGTGCCACTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACgg                                                                                                                                         >  1:7766502/1‑150 (MQ=255)

              gCGAAACCAGTTGCCCGAATCGTCGTGCCACTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGgtt                                                                                                                                       <  2:4522452/150‑1 (MQ=255)

                  aaCCAGTTGCCCGAATCGTCGTGCCACTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGttt                                                                                                                                   >  2:5495868/1‑150 (MQ=255)

                  aaCCAGTTGCCCGAATCGTCGTGCCACTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGttt                                                                                                                                   <  1:3912343/150‑1 (MQ=255)

                   aCCAGTTGCCCGAATCGTCGTGCCACTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTg                                                                                                                                  <  1:3710391/150‑1 (MQ=255)

                          gCCCGAATCGTCGTGCCACTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTc                                                                                                                           <  2:6170312/150‑1 (MQ=255)

                           cccGAATCGTCGTGCCACTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCg                                                                                                                          <  1:6233908/150‑1 (MQ=255)

                             cGAATCGTCGTGCCACTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGc                                                                                                                        >  1:5893351/1‑150 (MQ=255)

                                 tcgtcgTGCCACTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACgg                                                                                                                    <  1:2354739/150‑1 (MQ=255)

                                   gtcgtGCCACTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGtt                                                                                                                  <  2:1478352/150‑1 (MQ=255)

                                   ctcgtGCCACTCGGAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGtt                                                                                                                  <  2:8411738/149‑1 (MQ=255)

                                    tcgtGCCACTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTa                                                                                                                 <  1:2826069/150‑1 (MQ=255)

                                    tcgtGCCACTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTa                                                                                                                 <  1:5998934/150‑1 (MQ=255)

                                     cgtGCCACTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTAc                                                                                                                <  2:5220844/150‑1 (MQ=255)

                                      gtGCCACTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACg                                                                                                               <  1:6123952/150‑1 (MQ=255)

                                           aCTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCAtt                                                                                                          >  1:1906008/1‑150 (MQ=255)

                                           aCTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCAtt                                                                                                          >  1:9079699/1‑150 (MQ=255)

                                           aCTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCAtt                                                                                                          >  2:8040426/1‑150 (MQ=255)

                                             tCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTgg                                                                                                        <  2:3369344/150‑1 (MQ=255)

                                             tCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTgg                                                                                                        <  1:8963658/150‑1 (MQ=255)

                                               gTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGgt                                                                                                      <  2:3535757/150‑1 (MQ=255)

                                                 aGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGgtgt                                                                                                    >  1:2750473/1‑150 (MQ=255)

                                                      gaaCGGACCGCGAGGCGGTGGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTGCTTCCACTTGCGAGTTAGTAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCAcc                                                                                               <  2:6912048/149‑1 (MQ=255)

                                                         cGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGta                                                                                            <  2:2410153/150‑1 (MQ=255)

                                                         cGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGta                                                                                            <  2:1155338/150‑1 (MQ=255)

                                                            aCCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAg                                                                                         <  1:2980798/150‑1 (MQ=255)

                                                              cgcgATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTgg                                                                                                        >  1:7263176/1‑133 (MQ=255)

                                                              cgcgATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGc                                                                                       <  2:7396662/150‑1 (MQ=255)

                                                               gcgATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGcc                                                                                      >  2:3884055/1‑150 (MQ=255)

                                                                cgATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCa                                                                                     <  1:6555529/150‑1 (MQ=255)

                                                                 gATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAg                                                                                    >  2:1280795/1‑150 (MQ=255)

                                                                        ttGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGGTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCCTTGGGTGTCCACCGTATAGGCCAGGGCAATc                                                                             >  1:5159066/1‑150 (MQ=255)

                                                                         tGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCt                                                                            <  2:2565744/150‑1 (MQ=255)

                                                                            cGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCtttt                                                                         <  2:2835405/150‑1 (MQ=255)

                                                                             ggTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTc                                                                        <  2:9032888/150‑1 (MQ=255)

                                                                             ggTGAAGGCCCAGAGCACATTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTc                                                                        <  1:4771186/150‑1 (MQ=255)

                                                                               tGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCgg                                                                      <  2:5523737/150‑1 (MQ=255)

                                                                                      ccAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTcgcg                                                               >  1:101337/1‑150 (MQ=255)

                                                                                      ccAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTcgcg                                                               >  2:1855301/1‑150 (MQ=255)

                                                                                      ccAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTcgcg                                                               >  2:4892600/1‑150 (MQ=255)

                                                                                      ccAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTcgcg                                                               >  1:7934174/1‑150 (MQ=255)

                                                                                       cAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCgg                                                              <  2:8742533/150‑1 (MQ=255)

                                                                                         gagCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGt                                                            >  1:2434523/1‑150 (MQ=255)

                                                                                             tctTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTc                                                        <  2:7648900/150‑1 (MQ=255)

                                                                                             tctTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTc                                                        >  1:7437472/1‑150 (MQ=255)

                                                                                               tttGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCa                                                      >  1:8149253/1‑150 (MQ=255)

                                                                                               tttGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCa                                                      >  1:4674835/1‑150 (MQ=255)

                                                                                               tttGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCa                                                      <  1:4584052/150‑1 (MQ=255)

                                                                                                 tGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGc                                                    >  2:2270646/1‑150 (MQ=255)

                                                                                                 tGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGc                                                    <  1:7580482/150‑1 (MQ=255)

                                                                                                  gATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGcc                                                   <  1:1230019/150‑1 (MQ=255)

                                                                                                      aaCCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATcc                                               >  1:2875830/1‑150 (MQ=255)

                                                                                                      aaCCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATcc                                               >  2:8329264/1‑150 (MQ=255)

                                                                                                       aCCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCt                                              >  2:3175923/1‑150 (MQ=255)

                                                                                                       aCCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCt                                              >  2:6850791/1‑150 (MQ=255)

                                                                                                       aCCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCATTTCGGGTTCGCGGGTATTCCAGCCATCCt                                              >  2:6152350/1‑150 (MQ=255)

                                                                                                          gATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAg                                           >  2:8904025/1‑150 (MQ=255)

                                                                                                             aGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGa                                        <  2:6587529/150‑1 (MQ=255)

                                                                                                              gTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAg                                       >  2:4383047/1‑150 (MQ=255)

                                                                                                              gTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAg                                       >  1:8265565/1‑150 (MQ=255)

                                                                                                               tCCAGTTCCTTATTCCACTTGCGGGTCAGGAAGGCTTCGGCTTCGGCACGGTTTGTAACGAATTCAACGCGGTTGCGCCACCGCGTGTCCAAGGTATAGGCGAGAGCGACTTTCGCGGGATCGCGGGTATTCCAGCCATCTTCCGCAAgg                                      >  1:534974/1‑149 (MQ=255)

                                                                                                                ccAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTc                                     >  2:5882960/1‑150 (MQ=255)

                                                                                                                 cAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCg                                    >  1:1934175/1‑150 (MQ=255)

                                                                                                                 cAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCg                                    >  1:884670/1‑150 (MQ=255)

                                                                                                                    ttctttctTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGAc                                 >  1:4389425/1‑150 (MQ=255)

                                                                                                                    ttctttctTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGAc                                 <  2:1819196/150‑1 (MQ=255)

                                                                                                                       cttattcCACTTGCGGGTCAGGAAGGCTTCGGCTTCGGCACGGTTTGTAACGAATTCAACGCGGTTGCGCCACCGCGTGTCCAAGGTATAGGCGAGAGCGACTTTCGCGGGATCGCGGGTATTCCAGCCATCTTCCGCAAGGCGGACCtt                              >  2:1425408/5‑150 (MQ=255)

                                                                                                                         tcttcCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCtttt                            <  2:7090428/150‑1 (MQ=255)

                                                                                                                         tcttcCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCtttt                            <  2:159913/150‑1 (MQ=255)

                                                                                                                         tattcCACTTGCGGGTCAGGAAGGCTTCGGCTTCGGCACGGTTTGTAACGAATTCAACGCGGTTGCGCCACCGCGTGTCCAAGGTATAGGCGAGAGCGACTTTCGCGGGATCGCGGGTATTCCAGCCATCTTCCGCAAGGCGGACCtttt                            <  2:77904/148‑1 (MQ=255)

                                                                                                                           ttcCACTTGCGGGTCAGGAAGGCTTCGGCTTCGGCACGGTTTGTAACGAATTCAACGCGGTTGCGCCACCGCGTGTCCAAGGTATAGGCGAGAGCGACTTTCGCGGGATCGCGGGTATTCCAGCCATCTTCCGCAAGGCGGACCTTTTcg                          >  2:4754690/1‑149 (MQ=255)

                                                                                                                           ttcCACTTGCGGGTCAGGAAGGCTTCGGCTTCGGCACGGTTTGTAACGAATTCAACGCGGTTGCGCCACCGCGTGTCCAAGGTATAGGCGAGAGCGACTTTCGCGGGATCGCGGGTATTCCAGCCATCTTCCGCAAGGCGGACCTTTTcg                          >  2:7205101/1‑149 (MQ=255)

                                                                                                                           ttcCACTTGCGGGTCAGGAAGGCTTCGGCTTCGGCACGGTTTGTAACGAATTCAACGCGGTTGCGCCACCGCGTGTCCAAGGTATAGGCGAGAGCGACTTTCGCGGGATCGCGGGTATTCCAGCCATCTTCCGCAAGGCGGACCTTTTcg                          >  1:5175005/1‑149 (MQ=255)

                                                                                                                           ttcCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCa                          >  2:9088814/1‑150 (MQ=255)

                                                                                                                           ttcCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCa                          >  2:3652628/1‑150 (MQ=255)

                                                                                                                              cACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATc                       >  1:5771161/1‑150 (MQ=255)

                                                                                                                                   gCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGa                  >  2:8825412/1‑150 (MQ=255)

                                                                                                                                     gAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGAtt                >  1:139307/1‑150 (MQ=255)

                                                                                                                                     gAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGAtt                >  1:5477866/1‑150 (MQ=255)

                                                                                                                                        ttAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTcgc             <  1:4952041/150‑1 (MQ=255)

                                                                                                                                            gAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCtttt                            >  1:6636007/1‑131 (MQ=255)

                                                                                                                                            gAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGt         >  1:4596407/1‑150 (MQ=255)

                                                                                                                                            gAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGt         >  2:6693899/1‑150 (MQ=255)

                                                                                                                                                tCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGaaa     >  2:2047827/1‑150 (MQ=255)

                                                                                                                                                tCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGaaa     <  1:1712646/150‑1 (MQ=255)

                                                                                                                                                 cccGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAag    >  2:6421660/1‑150 (MQ=255)

                                                                                                                                                  ccGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAggg                                                                                  >  2:4198699/1‑71 (MQ=255)

                                                                                                                                                  ccGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAagg   >  2:7271746/1‑150 (MQ=255)

                                                                                                                                                   cGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAagga  >  2:4207656/1‑150 (MQ=255)

TCGTTGCCATAGGAGCGAAACCAGTTGCCCGAATCGTCGTGCCACTCGTAGGCGTAACGGACCGCGATGCGGTTGTCGGTGAAGGCCCAGAGCTCTTTGATCAACCGATAGTCCAGTTCTTTCTTCCACTTGCGAGTTAGGAAGTCCCGAGCTTCTTCACGGTTGTTTGCAAACTCGGCACGGTTACGCCATTGGGTGTCCACCGTATAGGCCAGGGCAATCTTTTCGGGTTCGCGGGTATTCCAGCCATCCTCAGCGAGTCGGACCTTTTCAATCGCCGATTCGCGAGTGAAAGGA  >  NC_002947/3356848‑3357144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`